FastQCFastQC Report
Mon 15 Aug 2016
SRR2921472_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921472_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3733893
Sequences flagged as poor quality0
Sequence length98
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT71470.19140880576920655No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40150.10752852317942693No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG120450.024.8787211
TACATGG123900.023.4568442
GAGTACA93850.021.973421
ACATGGG129050.021.6682343
AGTACAT90200.021.1416282
GTACCTG36950.021.0535891
TATATCG2059.620198E-720.1252045
CATGGGG97500.019.1355724
TACCTGG40800.018.3138262
GTATCAA143250.018.2197461
AGAGTAC102700.017.0394910-11
GTATACG2809.918549E-716.4397771
TATACCG3352.2639142E-816.4205655
GTACACG8750.016.3082561
GTATAGG8550.016.1513581
TCAACGC160450.016.1421134
TATCAAC163750.015.8727952
CAACGCA163150.015.8187795
ATCAACG165550.015.6725223
GTGTACG6350.015.2229721
Produced by FastQC (version 0.11.5)