##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921472_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3733893
Sequences flagged as poor quality	0
Sequence length	98
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.537517545360835	31.0	31.0	34.0	27.0	34.0
2	30.638338591920014	31.0	31.0	34.0	27.0	34.0
3	30.77939298206992	31.0	31.0	34.0	27.0	34.0
4	34.29351376699868	37.0	35.0	37.0	31.0	37.0
5	34.281887027828596	37.0	35.0	37.0	30.0	37.0
6	34.381788926463614	37.0	35.0	37.0	31.0	37.0
7	34.27942311148177	37.0	35.0	37.0	30.0	37.0
8	34.259277917176526	37.0	35.0	37.0	30.0	37.0
9	35.73508828453306	39.0	37.0	39.0	30.0	39.0
10-11	35.610612569776364	39.0	37.0	39.0	30.0	39.0
12-13	35.45932609745378	39.0	35.0	39.0	30.0	39.0
14-15	36.49015303866501	40.0	37.0	41.0	30.0	41.0
16-17	36.34566710936816	40.0	36.5	41.0	29.5	41.0
18-19	36.16721542368782	40.0	36.0	41.0	29.0	41.0
20-21	36.052826232567455	39.0	36.0	41.0	28.5	41.0
22-23	36.09591611221853	39.0	36.0	41.0	28.5	41.0
24-25	35.9676940394382	39.0	36.0	41.0	27.5	41.0
26-27	35.7030862962597	39.0	36.0	41.0	27.0	41.0
28-29	35.473446614565546	39.0	35.5	40.0	26.5	41.0
30-31	35.23101572541045	39.0	35.0	40.0	25.5	41.0
32-33	34.95751913619378	38.5	35.0	40.0	25.0	41.0
34-35	34.5347713499021	38.0	34.0	40.0	23.5	41.0
36-37	34.15293917099392	38.0	34.0	40.0	21.5	41.0
38-39	33.92004256683306	38.0	33.0	40.0	19.5	41.0
40-41	33.66011197964163	38.0	33.0	40.0	18.0	41.0
42-43	33.370700499451914	38.0	33.0	40.0	16.5	41.0
44-45	33.051944043388495	38.0	32.0	40.0	15.0	41.0
46-47	32.76313220544884	37.0	32.0	40.0	12.5	41.0
48-49	32.55034236385456	37.0	31.0	40.0	9.0	41.0
50-51	31.790731014520233	36.0	30.5	39.0	9.0	40.0
52-53	32.30634996771467	37.0	31.5	39.0	9.0	40.0
54-55	32.74344230003377	37.0	32.0	40.0	8.0	40.5
56-57	32.575345088892476	37.0	32.0	40.0	7.5	41.0
58-59	32.24021162363249	36.5	31.5	39.5	4.0	41.0
60-61	31.774355478317133	36.0	31.0	39.0	2.0	40.5
62-63	31.289178211587746	35.0	30.5	39.0	2.0	40.0
64-65	30.7490708758928	35.0	30.0	38.5	2.0	40.0
66-67	30.25060827934812	35.0	29.0	38.0	2.0	40.0
68-69	29.723027414015345	34.0	29.0	37.0	2.0	39.5
70-71	29.192615990870657	34.0	28.0	36.5	2.0	39.0
72-73	28.64029713759875	34.0	27.5	36.0	2.0	39.0
74-75	28.04146503394714	33.5	26.0	35.0	2.0	37.5
76-77	27.45691172725089	33.0	26.0	35.0	2.0	37.0
78-79	26.88413647632645	33.0	25.0	35.0	2.0	36.5
80-81	26.285907094820338	32.0	24.0	35.0	2.0	36.0
82-83	25.656946382769938	32.0	21.5	34.5	2.0	35.5
84-85	25.02812975626243	31.0	19.5	34.0	2.0	35.0
86-87	24.403086805111982	31.0	16.5	34.0	2.0	35.0
88-89	23.736421745347283	31.0	8.5	34.0	2.0	35.0
90-91	22.976012569187173	30.0	2.0	34.0	2.0	35.0
92-93	22.139374240236663	29.5	2.0	34.0	2.0	35.0
94-95	21.209207789296585	29.0	2.0	34.0	2.0	35.0
96-97	20.29801161950811	28.0	2.0	33.0	2.0	34.5
98	19.66301283941452	27.0	2.0	33.0	2.0	34.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	104807.0
3	18482.0
4	14387.0
5	12257.0
6	11574.0
7	12123.0
8	13795.0
9	16063.0
10	17990.0
11	19703.0
12	22109.0
13	25316.0
14	21488.0
15	21065.0
16	23180.0
17	26486.0
18	29996.0
19	30062.0
20	32107.0
21	34663.0
22	38306.0
23	42945.0
24	47697.0
25	53396.0
26	60920.0
27	70366.0
28	81479.0
29	95413.0
30	113898.0
31	138533.0
32	171689.0
33	221912.0
34	300234.0
35	417151.0
36	554678.0
37	569164.0
38	238618.0
39	9841.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.95164735916052	16.934350532021682	13.127579170822315	24.98642293799548
2	16.169061047053752	18.782167117890545	38.442211189923455	26.606560645132248
3	20.838702744824396	21.781816593497968	27.060869229674683	30.31861143200295
4	14.053454538828525	14.434526202059894	34.3267832203061	37.18523603880548
5	16.090179743646022	35.38597677436921	32.233877112063546	16.28996636992122
6	35.75674093091375	32.61381832214702	16.204845510981524	15.424595235957705
7	31.83218609645899	29.788326289867832	18.93665596638642	19.442831647286756
8	28.040909316164566	33.0666316648072	18.84839417808357	20.044064840944674
9	26.901235781495124	16.64923406832382	18.926096586326718	37.52343356385433
10-11	27.069237346156193	24.658964163915773	25.588736817406005	22.683061672522026
12-13	27.309710897675497	21.65309729914834	26.19701963628747	24.840172166888692
14-15	24.203898516011762	23.352926270947183	24.012620944693168	28.430554268347883
16-17	24.74796757549321	26.313211007060954	24.48435707457299	24.454464342872846
18-19	24.69472874677271	25.7953371589656	25.71060695178926	23.799327142472425
20-21	25.334971407633773	24.838201743940683	25.599646296032475	24.227180552393072
22-23	24.95540099960203	24.548303173743818	25.57638904992501	24.91990677672914
24-25	24.861972919597367	24.686486035955514	25.682993182329213	24.768547862117906
26-27	24.80584956178947	24.824920840122648	25.78438170862035	24.584847889467536
28-29	24.693332132488077	25.234047554812356	25.544592034938162	24.528028277761404
30-31	24.99031963039612	24.939686942876307	25.324262235250266	24.745731191477308
32-33	24.819659921718234	24.979170406170383	25.3745844127662	24.826585259345187
34-35	25.067585927606185	24.92717714618849	25.504153306006305	24.50108362019902
36-37	25.0273201362038	24.811804724601302	25.49484518993778	24.66602994925712
38-39	24.953241471198552	24.99208681098348	25.458405988755324	24.596265729062644
40-41	25.08908393951626	24.993915611040336	25.38541758841624	24.531582861027168
42-43	24.971841498030958	24.894189337430618	25.19985635629456	24.934112808243867
44-45	24.973626249353785	24.87202201447527	25.259065892641736	24.89528584352921
46-47	25.157505743329377	24.971401140831308	25.27132233747919	24.59977077836012
48-49	25.128262351358888	25.006851247438522	25.102607189044896	24.762279212157694
50-51	24.73010453615539	25.253976696540104	25.32097686979552	24.694941897508986
52-53	25.020532597249602	25.20606697830227	25.035056311052138	24.738344113395982
54-55	24.894541080793136	24.944443733580446	25.303823647661684	24.85719153796474
56-57	24.930523225208688	25.03861915095221	25.21824498745366	24.81261263638545
58-59	25.176963205329006	24.901160658954065	25.110401780642615	24.81147435507431
60-61	25.059909537641474	24.961474946498406	25.367086849006693	24.61152866685343
62-63	24.80376214445142	25.09944845254088	25.279429081196735	24.817360321810966
64-65	24.829985378648303	25.13416820560276	25.213603970630178	24.82224244511876
66-67	24.969878799968697	24.961689335633746	25.089811350031848	24.97862051436571
68-69	24.797257880436575	25.028384233324118	25.197902023806268	24.976455862433035
70-71	24.89456058860369	24.84857928954923	25.224342179451675	25.032517942395405
72-73	25.015855888340106	24.75585160987384	25.32965044392451	24.898642057861547
74-75	24.77111547359209	25.24518778202816	25.118312941260662	24.865383803119087
76-77	24.949932557277883	24.87348940007055	25.080714664143855	25.095863378507715
78-79	24.908076562954033	24.90478061065252	25.242272673461265	24.944870152932186
80-81	24.972937255192637	24.954843858018087	25.183789230890714	24.888429655898562
82-83	25.05303959105426	24.806207704222608	25.202174757783176	24.938577946939958
84-85	24.92744457346475	24.964097219765634	25.077458138306508	25.03100006846311
86-87	24.88696528939226	24.960299643201196	25.25022052764686	24.902514539759675
88-89	24.740381914134442	24.845690307483824	25.12885377323458	25.285074005147155
90-91	24.95200239342325	24.68015944675763	25.15285784852343	25.214980311295687
92-93	25.10720896196974	24.965631254977076	25.092102856035986	24.835056927017195
94-95	25.112373366395563	24.741620630313285	25.14355754732516	25.002448455965997
96-97	24.880921120600593	24.64459286107859	25.388365030305117	25.086120988015704
98	24.923850657432073	24.89756628799598	25.02341918484557	25.155163869726376
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	414.0
1	315.5
2	345.0
3	483.5
4	411.0
5	372.0
6	643.0
7	1390.0
8	2074.0
9	1974.5
10	1555.5
11	1469.5
12	1614.0
13	1850.0
14	2144.5
15	2410.0
16	2643.5
17	2727.5
18	2722.0
19	2665.0
20	2473.0
21	2294.0
22	2128.0
23	2050.0
24	2170.0
25	2365.5
26	2735.0
27	3293.5
28	4216.5
29	5808.5
30	8236.5
31	11932.0
32	17076.0
33	23176.5
34	30672.5
35	39475.5
36	49114.0
37	58819.0
38	68639.0
39	78501.0
40	88028.5
41	98223.0
42	109708.0
43	121910.0
44	133160.5
45	145483.5
46	162307.0
47	176924.5
48	181063.5
49	176522.5
50	172698.0
51	171956.0
52	171327.5
53	172210.0
54	170318.0
55	163124.0
56	152919.5
57	141943.0
58	130919.5
59	118194.5
60	104217.5
61	88850.0
62	72440.0
63	56348.0
64	42118.5
65	32077.5
66	24849.0
67	19019.5
68	14756.5
69	11489.5
70	8782.0
71	6872.0
72	5591.5
73	4374.5
74	3370.5
75	2549.5
76	1783.5
77	1156.5
78	686.5
79	417.5
80	253.0
81	158.0
82	85.5
83	51.0
84	35.5
85	22.0
86	18.5
87	12.0
88	6.0
89	8.0
90	9.5
91	8.5
92	9.5
93	7.0
94	3.0
95	4.0
96	6.0
97	6.0
98	3.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.48386496345771024
2	0.048769474647505966
3	0.056402258982782845
4	0.05487570211572747
5	0.06459745900592224
6	0.0642225152140139
7	0.04263646547986244
8	0.020300528161894302
9	0.012480271930663252
10-11	0.015024533375755544
12-13	0.011315268005805202
14-15	0.015399477167663884
16-17	0.014569244485581135
18-19	0.05484892041630545
20-21	0.08274206036434359
22-23	0.09931993230657656
24-25	0.06856115052038182
26-27	0.0844159165782201
28-29	0.08536666690770196
30-31	0.05661651257815904
32-33	0.032754018393135534
34-35	0.4046313057176518
36-37	0.6351949560418577
38-39	0.5821270186371168
40-41	0.5764359075099367
42-43	0.5500559335792429
44-45	0.5349108825560883
46-47	0.5244928014809209
48-49	0.5153066785791666
50-51	0.5282288485502933
52-53	0.6085069925678106
54-55	0.5802389088278641
56-57	0.5372944538046485
58-59	0.5163243831572035
60-61	0.52897873613411
62-63	0.5399190603480067
64-65	0.557862798960763
66-67	0.5840151284463695
68-69	0.5389415283191029
70-71	0.4888195778507847
72-73	0.4715721634230011
74-75	0.45111094506457466
76-77	0.46616226013975226
78-79	0.46099339215130164
80-81	0.4570966548853971
82-83	0.4767410314114518
84-85	0.44357189667727487
86-87	0.44650449276398657
88-89	0.4730987202900565
90-91	0.45638693985071344
92-93	0.45135198035937296
94-95	0.4623860405212468
96-97	0.45606555945764915
98	0.45140554375821695
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	3733893.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.6877481943872
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.29992628312132	42.685832601935
2	13.697987970720213	15.530161857078648
3	4.663353070054111	7.9306495373024966
4	2.0915780848246786	4.7426740720576
5	1.1689461171700983	3.3132461571472587
6	0.6816782360410985	2.31856825165751
7	0.4728251200963051	1.876237394360016
8	0.3282922377064809	1.488811816422152
9	0.2295864108524169	1.171326298252944
>10	1.2696381497668856	12.730315111256177
>50	0.07009872549154174	2.7005506152073897
>100	0.02466767116513258	2.266860699326297
>500	7.109615085403054E-4	0.2663209464487209
>1k	6.635640491506035E-4	0.7864152079706791
>5k	4.739743208218591E-5	0.1920294335770419
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	7147	0.19140880576920655	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4015	0.10752852317942693	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.6781699422024146E-5
2	0.0	0.0	0.0	0.0	2.6781699422024146E-5
3	0.0	0.0	0.0	0.0	2.6781699422024146E-5
4	0.0	0.0	0.0	0.0	2.6781699422024146E-5
5	0.0	0.0	0.0	0.0	2.6781699422024146E-5
6	0.0	0.0	0.0	0.0	8.034509826607243E-5
7	0.0	0.0	0.0	0.0	1.0712679768809658E-4
8	0.0	0.0	0.0	0.0	1.0712679768809658E-4
9	0.0	2.6781699422024146E-5	0.0	0.0	1.0712679768809658E-4
10-11	2.6781699422024146E-5	2.6781699422024146E-5	0.0	0.0	1.0712679768809658E-4
12-13	2.6781699422024146E-5	2.6781699422024146E-5	0.0	0.0	3.4816209248631386E-4
14-15	4.017254913303622E-5	2.6781699422024146E-5	0.0	0.0	4.0172549133036216E-4
16-17	6.695424855506036E-5	2.6781699422024146E-5	0.0	2.6781699422024146E-5	5.088522890184588E-4
18-19	8.034509826607243E-5	2.6781699422024146E-5	0.0	6.695424855506036E-5	5.49024838151495E-4
20-21	8.034509826607243E-5	2.6781699422024146E-5	0.0	1.0712679768809657E-4	6.427607861285795E-4
22-23	8.034509826607243E-5	2.6781699422024146E-5	0.0	1.7408104624315696E-4	7.231058843946518E-4
24-25	1.0712679768809658E-4	2.6781699422024146E-5	0.0	2.1425359537619317E-4	7.498875838166761E-4
26-27	1.0712679768809658E-4	2.6781699422024146E-5	0.0	3.079895433532777E-4	7.900601329497122E-4
28-29	1.2051764739910866E-4	2.6781699422024146E-5	0.0	0.0010444862774589417	8.034509826607243E-4
30-31	1.3390849711012072E-4	2.6781699422024146E-5	0.0	0.0031870222312208735	8.034509826607243E-4
32-33	1.3390849711012072E-4	2.6781699422024146E-5	0.0	0.008476407867070643	8.971869306378089E-4
34-35	1.6069019653214487E-4	2.6781699422024146E-5	0.0	0.015935111156104367	9.641411791928692E-4
36-37	1.6069019653214487E-4	2.6781699422024146E-5	0.0	0.026460319028959855	9.641411791928692E-4
38-39	1.8747189595416902E-4	2.6781699422024146E-5	0.0	0.0430381909711928	9.641411791928692E-4
40-41	1.8747189595416902E-4	2.6781699422024146E-5	0.0	0.061142619780481126	9.641411791928692E-4
42-43	1.8747189595416902E-4	2.6781699422024146E-5	0.0	0.07999693617358612	9.909228786148933E-4
44-45	1.8747189595416902E-4	2.6781699422024146E-5	0.0	0.09984217544530602	0.0010444862774589415
46-47	1.8747189595416902E-4	2.6781699422024146E-5	0.0	0.12257983825460451	0.00109804967630299
48-49	1.8747189595416902E-4	2.6781699422024146E-5	0.0	0.14446048668239825	0.0011516130751470382
50-51	1.8747189595416902E-4	2.6781699422024146E-5	0.0	0.166408089358747	0.0013123032716791832
52-53	1.8747189595416902E-4	2.6781699422024146E-5	0.0	0.18763258615070116	0.001446211768789304
54-55	1.8747189595416902E-4	2.6781699422024146E-5	0.0	0.20982122412184817	0.00148638431792234
56-57	1.8747189595416902E-4	2.6781699422024146E-5	0.0	0.23400509869993597	0.001513166017344364
58-59	1.8747189595416902E-4	2.6781699422024146E-5	0.0	0.2587112164167532	0.0015265568670553762
60-61	1.8747189595416902E-4	2.6781699422024146E-5	0.0	0.2824264112549556	0.0015399477167663883
62-63	1.8747189595416902E-4	2.6781699422024146E-5	0.0	0.3063558596885342	0.0016604653641654969
64-65	2.0086274566518108E-4	2.6781699422024146E-5	0.0	0.3307673787117092	0.0017274196127205575
66-67	2.1425359537619317E-4	2.6781699422024146E-5	0.0	0.35891494480425656	0.0017675921618535936
68-69	2.1425359537619317E-4	2.6781699422024146E-5	0.0	0.38572342592570275	0.0018211555606976418
70-71	2.1425359537619317E-4	2.6781699422024146E-5	0.0	0.41346926652691973	0.0018613281098306782
72-73	2.1425359537619317E-4	2.6781699422024146E-5	0.0	0.4390457894749528	0.002008627456651811
74-75	2.1425359537619317E-4	2.6781699422024146E-5	0.0	0.46726030981605526	0.0021693176531839557
76-77	2.1425359537619317E-4	2.6781699422024146E-5	0.0	0.4951266680646714	0.002249662751450028
78-79	2.1425359537619317E-4	2.6781699422024146E-5	0.0	0.5266621191341048	0.002289835300583064
80-81	2.1425359537619317E-4	2.6781699422024146E-5	0.0	0.5592688381804192	0.0023300078497161007
82-83	2.4103529479821732E-4	2.6781699422024146E-5	0.0	0.5909381977469628	0.002396962098271161
84-85	2.6781699422024144E-4	2.6781699422024146E-5	0.0	0.6223665220187081	0.002437134647404197
86	2.6781699422024144E-4	2.6781699422024146E-5	0.0	0.6469922946372593	0.002437134647404197
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACATG	12045	0.0	24.878721	1
TACATGG	12390	0.0	23.456844	2
GAGTACA	9385	0.0	21.97342	1
ACATGGG	12905	0.0	21.668234	3
AGTACAT	9020	0.0	21.141628	2
GTACCTG	3695	0.0	21.053589	1
TATATCG	205	9.620198E-7	20.125204	5
CATGGGG	9750	0.0	19.135572	4
TACCTGG	4080	0.0	18.313826	2
GTATCAA	14325	0.0	18.219746	1
AGAGTAC	10270	0.0	17.03949	10-11
GTATACG	280	9.918549E-7	16.439777	1
TATACCG	335	2.2639142E-8	16.420565	5
GTACACG	875	0.0	16.308256	1
GTATAGG	855	0.0	16.151358	1
TCAACGC	16045	0.0	16.142113	4
TATCAAC	16375	0.0	15.872795	2
CAACGCA	16315	0.0	15.818779	5
ATCAACG	16555	0.0	15.672522	3
GTGTACG	635	0.0	15.222972	1
>>END_MODULE