FastQCFastQC Report
Mon 15 Aug 2016
SRR2921472_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921472_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3733893
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT72260.19352456002354648No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG123900.026.2958321
TACATGG129050.024.2874262
ACATGGG129550.023.5705173
GTACCTG38050.021.7819481
CATGGGG94650.021.7753264
TACCTGG39350.020.8783862
GAGTACA96800.019.9288311
AGTACAT90200.019.4800822
GTGTACG5800.018.067451
GTATCAA145600.017.5349921
AGAGTAC104400.017.5077610-11
GTGTAGC20750.016.5278571
TATACTG12750.016.0095525
TCAACGC161700.015.5360734
CAACGCA163500.015.3298195
ACCTGGG50150.015.24581053
GTACGCT4753.074092E-1014.9966143
ATCAACG168050.014.920763
TATCAAC168550.014.9046752
ATGGGGC43200.014.8344315
Produced by FastQC (version 0.11.5)