##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921472_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3733893
Sequences flagged as poor quality	0
Sequence length	101
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.97205838517601	33.0	31.0	34.0	30.0	34.0
2	32.09020799471222	34.0	31.0	34.0	30.0	34.0
3	32.21878345201643	34.0	31.0	34.0	30.0	34.0
4	35.7436680697599	37.0	35.0	37.0	35.0	37.0
5	35.553612275445495	37.0	35.0	37.0	33.0	37.0
6	35.59021777003251	37.0	35.0	37.0	35.0	37.0
7	35.521828022388426	37.0	35.0	37.0	33.0	37.0
8	35.47369595218717	37.0	35.0	37.0	33.0	37.0
9	37.10127338946242	39.0	37.0	39.0	34.0	39.0
10-11	37.01599831596674	39.0	37.0	39.0	33.5	39.0
12-13	36.902861169294354	39.0	37.0	39.0	33.0	39.0
14-15	38.21694435271712	40.0	38.0	41.0	33.0	41.0
16-17	38.12894611602421	40.0	38.0	41.0	33.0	41.0
18-19	38.02132626724976	40.0	38.0	41.0	33.0	41.0
20-21	37.90370050775424	40.0	38.0	41.0	33.0	41.0
22-23	37.8726565008692	40.0	38.0	41.0	33.0	41.0
24-25	37.93017702971135	40.0	38.0	41.0	33.0	41.0
26-27	37.82891529028818	40.0	38.0	41.0	33.0	41.0
28-29	37.69872784249576	40.0	38.0	41.0	33.0	41.0
30-31	37.569798331125185	40.0	38.0	41.0	32.0	41.0
32-33	37.416303975502245	40.0	37.0	41.0	32.0	41.0
34-35	37.27748371471812	40.0	37.0	41.0	32.0	41.0
36-37	37.188622169944345	40.0	37.0	41.0	31.5	41.0
38-39	37.15836969618572	40.0	37.0	41.0	31.0	41.0
40-41	37.00664079018868	40.0	37.0	41.0	31.0	41.0
42-43	36.774419218761764	40.0	36.0	41.0	30.5	41.0
44-45	36.582095550140295	39.0	36.0	41.0	30.0	41.0
46-47	36.33612666458305	39.0	35.5	41.0	30.0	41.0
48-49	36.022440252037214	39.0	35.0	40.0	29.5	41.0
50-51	35.752759117628706	39.0	35.0	40.0	28.5	41.0
52-53	35.44693661548416	38.0	35.0	40.0	28.0	41.0
54-55	35.125549526995016	38.0	34.0	40.0	27.5	41.0
56-57	34.74614912639436	38.0	34.0	40.0	26.5	41.0
58-59	34.36460217258502	37.0	34.0	40.0	26.0	41.0
60-61	33.93479151652176	36.5	33.0	39.5	26.0	41.0
62-63	33.50382107896504	36.0	33.0	39.0	24.5	40.0
64-65	33.000381907033756	35.5	32.0	39.0	23.0	40.0
66-67	32.47797272176787	35.0	31.5	38.0	22.0	40.0
68-69	31.980857512521116	35.0	31.0	37.5	20.5	39.5
70-71	31.43422548530448	34.5	31.0	37.0	19.5	39.0
72-73	30.901921399461635	34.0	30.0	36.0	18.0	39.0
74-75	30.390290241311146	34.0	29.5	36.0	16.0	38.0
76-77	29.087172155174237	32.5	28.0	34.5	13.0	36.0
78-79	30.031700961971865	34.0	30.0	35.0	14.0	37.0
80-81	30.067770956478935	34.0	30.0	35.0	14.0	36.5
82-83	29.743476044975043	34.0	30.0	35.0	8.5	36.0
84-85	29.267055456597177	34.0	29.0	35.0	6.0	35.5
86-87	28.78022187566703	34.0	29.0	35.0	2.0	35.0
88-89	28.244657653553546	33.0	28.0	35.0	2.0	35.0
90-91	27.78849085927208	33.0	27.0	35.0	2.0	35.0
92-93	27.36993039168503	33.0	26.5	34.5	2.0	35.0
94-95	26.89072289966531	32.5	25.5	34.0	2.0	35.0
96-97	26.144093577400316	32.0	24.5	34.0	2.0	35.0
98-99	25.16541355095071	32.0	21.0	34.0	2.0	35.0
100-101	21.560328857843544	28.0	2.0	32.5	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	24566.0
3	12282.0
4	7238.0
5	5457.0
6	4793.0
7	4830.0
8	5677.0
9	6704.0
10	7846.0
11	9336.0
12	11106.0
13	12660.0
14	14112.0
15	15798.0
16	17585.0
17	19890.0
18	20611.0
19	19320.0
20	19672.0
21	21147.0
22	22304.0
23	25366.0
24	28900.0
25	33980.0
26	40420.0
27	49129.0
28	59986.0
29	73292.0
30	90882.0
31	113537.0
32	146558.0
33	198642.0
34	283720.0
35	431863.0
36	675058.0
37	831461.0
38	357717.0
39	10448.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.28165982100707	17.3660058525083	13.490566316560459	24.86176800992417
2	16.311260124486697	19.00504379745215	38.119330146846735	26.564365931214418
3	20.819533208951427	21.92386455978072	27.317095120018898	29.939507111248957
4	14.330997606212431	14.63905740561448	34.28612313334546	36.74382185482762
5	16.178567449553707	35.30635222632163	32.32871935620717	16.186360967917487
6	35.892997736641064	32.690315364203556	16.151153573432676	15.265533325722702
7	31.91828248166027	29.729227035272952	18.976512927055964	19.37597755601081
8	28.14524147889116	33.02578282355067	18.84846033062216	19.980515366936014
9	26.82674139298572	16.699418403143092	18.986214248627096	37.487625955244084
10-11	27.11208663832568	24.591461864256274	25.716299666698344	22.580151830719696
12-13	27.191876317058572	21.651095854386835	26.32837110029362	24.828656728260977
14-15	24.164449228218867	23.368154452967907	24.05512601781195	28.412270301001275
16-17	24.717329615476118	26.29273946208236	24.599289077687068	24.390641844754455
18-19	24.667790130220858	25.81561176743233	25.77704517475529	23.73955292759152
20-21	25.2973346136696	24.822927966886635	25.625781318332912	24.253956101110845
22-23	24.958384741205737	24.55292231148549	25.64901183017384	24.83968111713493
24-25	24.860951746150302	24.68400194283115	25.701443219205842	24.753603091812703
26-27	24.800175294463358	24.79677333895504	25.85539760421971	24.54765376236189
28-29	24.747957130444835	25.206979127363972	25.61992614341293	24.425137598778264
30-31	24.909367840688805	24.91596974000033	25.42679337313935	24.747869046171513
32-33	24.737798830590418	24.94828827009499	25.515985707394773	24.79792719191982
34-35	25.04002505893443	24.89062495605781	25.592976855974204	24.476373129033558
36-37	24.92368154725276	24.8413332838717	25.584663965115084	24.65032120376046
38-39	24.856047710437394	25.012609301821016	25.587347037718082	24.543995950023504
40-41	25.036021462603976	24.980840787412163	25.449729199497163	24.5334085504867
42-43	24.908768568848647	24.909491697876156	25.283496709160318	24.89824302411488
44-45	24.88883454460667	24.790867517776697	25.37900449429595	24.941293443320685
46-47	25.005528356147483	24.9753383749084	25.402565451919507	24.616567817024603
48-49	25.04548977638367	24.987318569845353	25.276219492924913	24.690972160846066
50-51	24.70467710367889	25.267836517311732	25.4053018915048	24.62218448750458
52-53	25.02955419894596	25.122140393559622	25.08656019120375	24.76174521629067
54-55	24.935175857122942	24.863746434387576	25.318784264261545	24.882293444227937
56-57	24.933043689260582	25.026702176722882	25.265776379849044	24.774477754167496
58-59	25.201142787509685	24.907116711225203	25.157260497033562	24.734480004231553
60-61	25.03065652571874	24.981898019595803	25.400342688868903	24.587102765816553
62-63	24.79513677257935	25.113880611147117	25.320675720866646	24.770306895406886
64-65	24.89680389365096	25.10836528582616	25.197044836337884	24.79778598418499
66-67	24.995157428451172	24.97030827154087	25.052236812599315	24.982297487408644
68-69	24.837523543258968	25.032100028552712	25.2194009986616	24.910975429526722
70-71	24.934638675958094	24.855664036500023	25.227448836567092	24.98224845097479
72-73	25.076019415642058	24.862290364765943	25.272469043377647	24.78922117621435
74-75	24.770435094826986	25.354469113572662	25.070658028005983	24.80443776359437
76-77	24.935134222495154	24.899109991969095	25.106887330525268	25.05886845501048
78-79	24.92888759643694	24.93616433913641	25.270572879325343	24.864375185101306
80-81	25.012179542735026	25.042934813694185	25.179190308553327	24.765695335017462
82-83	25.04265449311407	24.927409004417743	25.213111732671184	24.816824769797
84-85	24.89093122646955	25.112618012185273	25.085041097504952	24.911409663840224
86-87	24.861464175486443	25.117426816631568	25.221745133767026	24.799363874114967
88-89	24.753257820688095	25.013789236385275	25.100664454734485	25.132288488192145
90-91	24.895720829889093	24.936320955524224	25.106243476057255	25.061714738529428
92-93	25.06961899992471	25.147242878605308	25.16877231820485	24.614365803265134
94-95	25.05778602004177	25.001178486619622	25.08829275321794	24.852742740120668
96-97	24.79248731984131	24.944910360066288	25.352121729523425	24.910480590568977
98-99	24.818703989650558	25.05601259931941	25.134410150165998	24.99087326086403
100-101	25.272750209179467	24.490309178505886	25.24786829782014	24.989072314494504
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	152.0
1	119.0
2	212.5
3	344.0
4	263.5
5	213.0
6	454.0
7	1170.5
8	1717.0
9	1561.0
10	1089.5
11	720.5
12	655.0
13	694.5
14	756.0
15	787.0
16	844.5
17	930.0
18	985.5
19	1035.5
20	1046.0
21	1107.5
22	1191.0
23	1265.5
24	1392.0
25	1605.0
26	1892.0
27	2353.5
28	3061.5
29	4235.5
30	6283.0
31	9386.0
32	13686.0
33	19078.0
34	25466.5
35	33126.0
36	42145.0
37	51862.0
38	61399.5
39	70316.5
40	79866.5
41	89689.0
42	100343.0
43	111538.5
44	122944.5
45	133991.5
46	145569.5
47	161276.0
48	180370.0
49	184441.0
50	173769.5
51	169748.0
52	169846.0
53	169247.0
54	168402.5
55	162981.5
56	152303.5
57	140394.0
58	129701.5
59	117096.5
60	102353.0
61	89030.0
62	74176.5
63	56838.5
64	42229.5
65	32212.0
66	24922.0
67	19470.5
68	14923.0
69	11430.5
70	8896.5
71	7026.5
72	5486.5
73	4442.0
74	3363.0
75	2409.5
76	1826.0
77	1202.0
78	722.5
79	392.0
80	214.0
81	120.5
82	65.5
83	39.0
84	25.5
85	12.5
86	5.0
87	4.5
88	3.0
89	2.0
90	1.5
91	0.0
92	0.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.32325511202383145
2	0.0
3	0.006186572566487577
4	0.05758065375735191
5	5.62415687862507E-4
6	0.060821239387416834
7	0.07094472176894197
8	0.10107413361871913
9	0.08843317149152373
10-11	0.09342795843373122
12-13	0.09416445516783689
14-15	0.06153095442210047
16-17	0.03420023016192483
18-19	0.03717299879776951
20-21	0.02730394256075362
22-23	0.03985116873997193
24-25	0.03203091250874088
26-27	0.019979147768830013
28-29	0.022697490260165463
30-31	0.0031870222312208735
32-33	0.005731283676313167
34-35	0.00788721047978611
36-37	0.00962802094221768
38-39	0.01440855428904899
40-41	0.018800752994260952
42-43	0.0032004130809318854
44-45	0.013230159514479927
46-47	0.023206342549183922
48-49	0.0023300078497161007
50-51	0.006039273219666444
52-53	0.002008627456651811
54-55	0.0036824836705283202
56-57	0.003079895433532777
58-59	0.0010846588265919779
60-61	0.0049680052427854785
62-63	0.013056078468236771
64-65	0.006025882369955432
66-67	0.03674449160701712
68-69	0.05887956617932008
70-71	0.011141186959562044
72-73	0.03024992949717627
74-75	0.04909085504057025
76-77	0.3052712008619422
78-79	0.07573864596548428
80-81	0.03212464845671796
82-83	0.026567445826647954
84-85	0.018693626196572853
86-87	0.11897769968234227
88-89	0.5341743858219825
90-91	0.12963681605230787
92-93	0.048046368763111316
94-95	0.007793474531809026
96-97	0.005503639231225961
98-99	0.009587848393084644
100-101	0.006882896751460206
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3733893.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.94944031300916
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.96045679006944	38.10265911702657
2	15.285612691062148	16.187292736663412
3	5.361042349134981	8.515925756431113
4	2.420229255466045	5.125991380243918
5	1.3232368698306232	3.5032325829534834
6	0.8382214824519852	2.663001501250407
7	0.5465411007979395	2.0257331776714778
8	0.3664595347331941	1.5523061809190697
9	0.29464707065616763	1.4041257733000567
>10	1.486599724570574	13.955068541650123
>50	0.08390832195484381	3.036418320759899
>100	0.03137485722357624	2.66601964862478
>500	9.108829553399426E-4	0.3147055930344096
>1k	7.08464486983393E-4	0.7539000617319679
>5k	5.060460621309947E-5	0.1936196277393801
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	7226	0.19352456002354648	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0712679768809658E-4
2	0.0	0.0	0.0	2.6781699422024146E-5	1.0712679768809658E-4
3	0.0	0.0	0.0	2.6781699422024146E-5	1.0712679768809658E-4
4	0.0	2.6781699422024146E-5	0.0	2.6781699422024146E-5	1.0712679768809658E-4
5	2.6781699422024146E-5	2.6781699422024146E-5	0.0	2.6781699422024146E-5	1.3390849711012072E-4
6	2.6781699422024146E-5	2.6781699422024146E-5	0.0	2.6781699422024146E-5	1.3390849711012072E-4
7	2.6781699422024146E-5	2.6781699422024146E-5	0.0	2.6781699422024146E-5	1.3390849711012072E-4
8	2.6781699422024146E-5	2.6781699422024146E-5	0.0	2.6781699422024146E-5	1.8747189595416902E-4
9	2.6781699422024146E-5	2.6781699422024146E-5	0.0	2.6781699422024146E-5	1.8747189595416902E-4
10-11	8.034509826607243E-5	2.6781699422024146E-5	0.0	2.6781699422024146E-5	2.410352947982173E-4
12-13	1.0712679768809657E-4	2.6781699422024146E-5	0.0	2.6781699422024146E-5	5.624156878625071E-4
14-15	1.3390849711012072E-4	2.6781699422024146E-5	0.0	4.017254913303622E-5	6.159790867065554E-4
16-17	1.3390849711012072E-4	2.6781699422024146E-5	0.0	6.695424855506036E-5	6.829333352616157E-4
18-19	1.3390849711012072E-4	2.6781699422024146E-5	0.0	9.373594797708451E-5	7.23105884394652E-4
20-21	1.3390849711012072E-4	2.6781699422024146E-5	0.0	1.3390849711012072E-4	7.36496734105664E-4
22-23	1.3390849711012072E-4	2.6781699422024146E-5	0.0	2.0086274566518108E-4	7.900601329497122E-4
24-25	1.4729934682113278E-4	2.6781699422024146E-5	0.0	2.410352947982173E-4	8.570143815047727E-4
26-27	1.6069019653214487E-4	4.017254913303622E-5	0.0	3.347712427753018E-4	8.837960809267968E-4
28-29	2.2764444508720523E-4	5.356339884404829E-5	0.0	0.0010712679768809658	9.105777803488209E-4
30-31	2.6781699422024144E-4	5.356339884404829E-5	0.0	0.003133458832376825	9.641411791928692E-4
32-33	2.6781699422024144E-4	5.356339884404829E-5	0.0	0.008369281069382546	9.909228786148933E-4
34-35	2.6781699422024144E-4	5.356339884404829E-5	0.0	0.01577442095957222	0.0010177045780369175
36-37	2.6781699422024144E-4	5.356339884404829E-5	0.0	0.026138938635895566	0.0010177045780369175
38-39	2.6781699422024144E-4	5.356339884404829E-5	0.0	0.042462384433619285	0.0010444862774589417
40-41	2.6781699422024144E-4	5.356339884404829E-5	0.0	0.06048646814464153	0.0010444862774589417
42-43	2.8120784393125355E-4	5.356339884404829E-5	0.0	0.07943452048572361	0.0011248313757250142
44-45	2.945986936422656E-4	5.356339884404829E-5	0.0	0.09921280550888845	0.0012453490231241228
46-47	2.945986936422656E-4	5.356339884404829E-5	0.0	0.12187012321992088	0.0013256941213901953
48-49	2.945986936422656E-4	5.356339884404829E-5	0.0	0.14365703569973753	0.0014060392196562676
50-51	2.945986936422656E-4	5.356339884404829E-5	0.0	0.16544394817955416	0.001446211768789304
52-53	2.945986936422656E-4	5.356339884404829E-5	0.0	0.18605246588480173	0.0015935111156104367
54-55	2.945986936422656E-4	5.356339884404829E-5	0.0	0.20755817052068712	0.0016604653641654969
56-57	2.945986936422656E-4	5.356339884404829E-5	0.0	0.23123319280975646	0.001673856213876509
58-59	2.945986936422656E-4	5.356339884404829E-5	0.0	0.2555375850352434	0.001687247063587521
60-61	3.2138039306428974E-4	5.356339884404829E-5	0.0	0.27878410013356036	0.0017140287630095453
62-63	3.2138039306428974E-4	5.356339884404829E-5	0.0	0.3019636609833222	0.0017943738612756176
64-65	3.2138039306428974E-4	5.356339884404829E-5	0.0	0.3258127643186347	0.0018211555606976418
66-67	3.347712427753018E-4	5.356339884404829E-5	0.0	0.35331756962505356	0.001847937260119666
68-69	3.4816209248631386E-4	5.356339884404829E-5	0.0	0.37942972656152707	0.001847937260119666
70-71	3.4816209248631386E-4	5.356339884404829E-5	0.0	0.4063319436309504	0.0019684549075187747
72-73	3.4816209248631386E-4	5.356339884404829E-5	0.0	0.43119875154429976	0.002236271901739016
74-75	3.615529421973259E-4	5.356339884404829E-5	0.0	0.4589981555443608	0.002396962098271161
76-77	3.7494379190833804E-4	5.356339884404829E-5	0.0	0.4859137634634951	0.002423743797693185
78-79	3.7494379190833804E-4	5.356339884404829E-5	0.0	0.5172215700878413	0.0025040888959592575
80-81	4.5528889017441046E-4	5.356339884404829E-5	0.0	0.5495738629896465	0.0025576522948033056
82-83	4.686797398854225E-4	5.356339884404829E-5	0.0	0.5808013245157266	0.002758515040468487
84-85	4.820705895964346E-4	8.034509826607243E-5	0.0	0.6119752226429627	0.002812078439312535
86-87	4.820705895964346E-4	8.034509826607243E-5	0.0	0.6442739521459238	0.0028924235375786074
88-89	4.820705895964346E-4	8.034509826607243E-5	0.0	0.6778046398222981	0.00297276863584468
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACATG	12390	0.0	26.295832	1
TACATGG	12905	0.0	24.287426	2
ACATGGG	12955	0.0	23.570517	3
GTACCTG	3805	0.0	21.781948	1
CATGGGG	9465	0.0	21.775326	4
TACCTGG	3935	0.0	20.878386	2
GAGTACA	9680	0.0	19.928831	1
AGTACAT	9020	0.0	19.480082	2
GTGTACG	580	0.0	18.06745	1
GTATCAA	14560	0.0	17.534992	1
AGAGTAC	10440	0.0	17.50776	10-11
GTGTAGC	2075	0.0	16.527857	1
TATACTG	1275	0.0	16.009552	5
TCAACGC	16170	0.0	15.536073	4
CAACGCA	16350	0.0	15.329819	5
ACCTGGG	5015	0.0	15.2458105	3
GTACGCT	475	3.074092E-10	14.996614	3
ATCAACG	16805	0.0	14.92076	3
TATCAAC	16855	0.0	14.904675	2
ATGGGGC	4320	0.0	14.834431	5
>>END_MODULE