FastQCFastQC Report
Mon 15 Aug 2016
SRR2921471_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921471_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5093669
Sequences flagged as poor quality0
Sequence length98
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55890.1097244442071128No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA90400.039.0068471
GGTATCA52950.031.9066071
TCAACGC110750.031.2533344
CAACGCA111600.031.0152955
TATCAAC111700.030.9464862
ATCAACG114550.030.0164493
AACGCAG120000.028.8739176
GTACATG174100.025.8330881
TACATGG182750.024.3085772
ACGCAGA143500.024.0122577
ACATGGG181600.023.4274643
CGCAGAG154900.022.0340928
GAGTACA132200.020.6144051
CATGGGG140200.020.0776794
AGTACAT125800.020.0435732
GCAGAGT177800.018.886619
GTATAGG10500.017.9752561
AGAGTAC142900.017.66440410-11
GTATATA9500.017.4445361
GTACCTG42400.016.8285371
Produced by FastQC (version 0.11.5)