##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921471_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5093669
Sequences flagged as poor quality	0
Sequence length	98
%GC	50
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.78165856477914	31.0	31.0	34.0	28.0	34.0
2	30.888494913980473	31.0	31.0	34.0	27.0	34.0
3	31.029560420985344	33.0	31.0	34.0	28.0	34.0
4	34.54202128171265	37.0	35.0	37.0	32.0	37.0
5	34.5367796768891	37.0	35.0	37.0	32.0	37.0
6	34.637285814998975	37.0	35.0	37.0	32.0	37.0
7	34.5337755554984	37.0	35.0	37.0	32.0	37.0
8	34.51463395049816	37.0	35.0	37.0	32.0	37.0
9	36.019624557465356	39.0	37.0	39.0	32.0	39.0
10-11	35.9072962927116	39.0	37.0	39.0	31.0	39.0
12-13	35.76583873039257	39.0	37.0	39.0	30.0	39.0
14-15	36.86424746091668	40.0	37.0	41.0	31.0	41.0
16-17	36.732857690595914	40.0	37.0	41.0	30.5	41.0
18-19	36.55574174529205	40.0	37.0	41.0	30.0	41.0
20-21	36.441088633753	40.0	37.0	41.0	30.0	41.0
22-23	36.490796025419	40.0	37.0	41.0	30.0	41.0
24-25	36.37926168740057	40.0	37.0	41.0	30.0	41.0
26-27	36.13692075397911	39.5	36.0	41.0	29.0	41.0
28-29	35.92124998699366	39.0	36.0	41.0	27.5	41.0
30-31	35.68360998722139	39.0	36.0	40.0	27.0	41.0
32-33	35.431020252788315	39.0	35.0	40.0	26.5	41.0
34-35	35.030172553418765	38.5	35.0	40.0	25.0	41.0
36-37	34.67137077419047	38.0	34.0	40.0	24.0	41.0
38-39	34.46097891323524	38.0	34.0	40.0	23.5	41.0
40-41	34.203264876457425	38.0	33.5	40.0	22.5	41.0
42-43	33.92398249670326	38.0	33.0	40.0	20.5	41.0
44-45	33.61929652280115	38.0	33.0	40.0	19.0	41.0
46-47	33.350669625372205	38.0	33.0	40.0	18.0	41.0
48-49	33.16153366070705	38.0	32.5	40.0	15.5	41.0
50-51	32.37770603076093	36.5	31.0	39.0	15.0	40.0
52-53	32.83923602024396	37.0	32.5	39.0	17.5	40.0
54-55	33.269110831504754	38.0	33.0	40.0	16.5	41.0
56-57	33.11867359265001	37.0	33.0	40.0	14.5	41.0
58-59	32.79270345206962	37.0	32.5	40.0	11.0	41.0
60-61	32.34515061736442	36.0	32.0	39.0	8.0	41.0
62-63	31.85978633476184	35.5	31.0	39.0	6.5	40.0
64-65	31.323517193598562	35.0	31.0	38.5	2.0	40.0
66-67	30.845632195574545	35.0	30.0	38.0	2.0	40.0
68-69	30.334813373228613	35.0	30.0	37.0	2.0	39.5
70-71	29.831118001581963	34.0	29.0	36.5	2.0	39.0
72-73	29.289207740039643	34.0	29.0	36.0	2.0	39.0
74-75	28.708465253631516	34.0	28.0	35.5	2.0	38.0
76-77	28.144758031980484	33.5	27.0	35.0	2.0	37.0
78-79	27.605758246167937	33.0	26.0	35.0	2.0	36.5
80-81	27.053029260440752	33.0	25.5	35.0	2.0	36.0
82-83	26.450970017879058	32.0	24.5	35.0	2.0	35.5
84-85	25.86254309025577	32.0	23.5	34.0	2.0	35.0
86-87	25.262694434993715	31.5	20.5	34.0	2.0	35.0
88-89	24.631613184916414	31.0	18.5	34.0	2.0	35.0
90-91	23.913033905422594	31.0	11.0	34.0	2.0	35.0
92-93	23.12087662154726	30.0	2.0	34.0	2.0	35.0
94-95	22.225631956061534	29.5	2.0	34.0	2.0	35.0
96-97	21.33495767000172	29.0	2.0	34.0	2.0	35.0
98	20.705134157716177	29.0	2.0	33.0	2.0	34.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	122858.0
3	22681.0
4	17623.0
5	15141.0
6	14277.0
7	14960.0
8	16730.0
9	19690.0
10	22005.0
11	24373.0
12	27473.0
13	33010.0
14	26647.0
15	25765.0
16	28949.0
17	32621.0
18	37454.0
19	37024.0
20	39169.0
21	42513.0
22	46608.0
23	51591.0
24	58200.0
25	66306.0
26	75704.0
27	87460.0
28	101579.0
29	119189.0
30	143509.0
31	175272.0
32	221382.0
33	290153.0
34	399210.0
35	575643.0
36	802648.0
37	869112.0
38	374637.0
39	14503.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.34686981248761	16.637086441525547	12.963062692520513	25.052981053466333
2	16.464836157939075	18.206838411652527	38.372388337180205	26.955937093228194
3	20.826286177202142	21.288049241107228	26.964413187483228	30.921251394207406
4	14.255399309661518	14.075782445162249	33.57582133857779	38.09299690659845
5	16.283622414871214	35.27676174195045	32.12025891511276	16.31935692806558
6	36.59415293184393	32.08373261856856	15.961115413372687	15.360999036214826
7	32.45596117072343	29.566882768958575	18.378384323732337	19.598771736585658
8	28.60668694441554	33.255344509004225	18.29837745281889	19.839591093761353
9	27.12046510805987	16.389698822503785	18.395094452985564	38.09474161645078
10-11	27.37826200797994	24.496069965713996	25.32695307157845	22.79871495472761
12-13	27.307443592539983	21.42548391710861	26.14052133490055	25.126551155450848
14-15	24.389890225156584	23.16713279552532	23.479935089179726	28.96304189013837
16-17	25.00553713987483	26.220311014470788	24.137855540340418	24.636296305313966
18-19	25.00172123700024	25.509105564717483	25.496101754227784	23.993071444054497
20-21	25.45965950565542	24.709251547098702	25.495793679565132	24.335295267680742
22-23	25.265142437263666	24.351923445209266	25.39062050920908	24.992313608317986
24-25	24.97049140262497	24.599855954037313	25.465860596042234	24.96379204729548
26-27	24.904406496978538	24.873950190774373	25.433422711129992	24.788220601117096
28-29	24.96545597523205	24.877789213854236	25.415206208964236	24.74154860194948
30-31	25.1481124435668	24.87491510158099	25.1785899324103	24.798382522441912
32-33	24.967428865900015	24.838735081301163	25.187747990350225	25.006088062448594
34-35	25.105711037312467	24.78308836283702	25.37003790853384	24.741162691316674
36-37	25.102618300225675	24.763593158766813	25.286755349319073	24.847033191688435
38-39	25.089990678822797	24.84441749751124	25.261239294986876	24.804352528679082
40-41	25.242447766033056	24.78250511966081	25.270584551088454	24.70446256321768
42-43	25.21112435321376	24.674399725617054	25.16672958721652	24.94774633395267
44-45	25.045169937550078	24.82126678323828	25.195178833874003	24.93838444533764
46-47	25.15139991265409	24.719939517000384	25.24151855587563	24.887142014469898
48-49	25.167983324948512	24.878068224568963	25.01071247346301	24.943235977019512
50-51	24.968640084878995	25.09359622244265	25.013686658990824	24.924077033687535
52-53	25.14700304005279	24.941518264561722	24.957200959014543	24.954277736370944
54-55	25.14624172827166	24.851477641231746	25.095593960100615	24.906686670395974
56-57	25.011928628560515	24.94031244865262	25.134496212197842	24.91326271058902
58-59	25.128192327117834	24.882271043832002	25.093452554261287	24.89608407478888
60-61	25.199199531086258	24.83539667221232	25.11534370232661	24.85006009437481
62-63	25.050213050182258	24.98717040777576	25.09703119288364	24.86558534915834
64-65	25.08360520654138	24.824320488072786	25.106455980230084	24.98561832515575
66-67	25.08733001008664	24.914832259241678	25.03605756889049	24.96178016178119
68-69	24.954426006506285	24.883104384380022	25.127001239123132	25.03546836999056
70-71	24.91980292262395	24.76060227950859	25.227038463625018	25.09255633424244
72-73	25.083970637052566	24.84025904120513	25.02557729134956	25.050193030392748
74-75	24.946072116496257	25.025900272979555	25.01926436026794	25.008763250256244
76-77	25.121319956391975	24.804417211068472	24.988888434159225	25.085374398380328
78-79	24.995942191563664	24.799540318838233	25.119471149721594	25.08504633987651
80-81	25.00566745457186	24.88411423576828	25.113997434435266	24.99622087522459
82-83	25.168882567168875	24.81540627976662	24.997075733280838	25.018635419783664
84-85	25.06613714880661	24.923017463046804	24.89478001609061	25.116065372055967
86-87	25.028351339301295	24.855423451975465	25.15440658985496	24.96181861886828
88-89	25.004366523680073	24.683240882693624	25.096637998667603	25.2157545949587
90-91	25.030119192921983	24.745986096164092	25.07714437905554	25.146750331858385
92-93	25.090769901713227	24.817713291644043	25.095394303190943	24.996122503451783
94-95	25.16002987151953	24.737832843544417	25.0122895091819	25.089847775754155
96-97	25.126608259417093	24.621841671316787	25.050148792253	25.201401277013126
98	25.098719369584337	24.760991058856938	24.93610652068453	25.204183050874196
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	670.0
1	499.5
2	480.5
3	680.0
4	605.0
5	545.5
6	962.0
7	1997.5
8	2968.0
9	2962.5
10	2335.0
11	1979.5
12	2138.0
13	2521.0
14	2918.0
15	3359.5
16	3686.0
17	3802.0
18	3822.5
19	3684.0
20	3439.0
21	3111.0
22	2814.5
23	2692.0
24	2710.0
25	2855.0
26	3210.5
27	3833.5
28	4838.5
29	6382.0
30	8917.5
31	12685.5
32	18218.5
33	25487.0
34	34476.0
35	45697.0
36	58989.0
37	73692.5
38	88363.0
39	102391.0
40	117420.0
41	133507.0
42	149802.0
43	166841.0
44	182740.5
45	198015.0
46	213482.0
47	226539.0
48	234450.0
49	237612.5
50	238502.0
51	239232.0
52	242130.0
53	244780.0
54	242277.0
55	233406.0
56	219697.5
57	205207.5
58	189879.0
59	169661.5
60	147729.0
61	125480.5
62	102043.0
63	78467.0
64	58311.0
65	44592.0
66	34216.5
67	26161.0
68	20216.0
69	15591.0
70	11769.0
71	8934.5
72	7049.5
73	5485.0
74	4397.5
75	3365.0
76	2294.0
77	1444.5
78	885.0
79	530.5
80	316.5
81	200.0
82	121.0
83	67.0
84	46.5
85	40.0
86	27.0
87	22.5
88	21.5
89	15.5
90	11.5
91	10.0
92	9.0
93	8.5
94	7.0
95	7.5
96	7.0
97	4.5
98	1.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.47910455115948836
2	0.049394650496528136
3	0.0581702501674137
4	0.05561806234366623
5	0.06580718142462731
6	0.06523784721779134
7	0.044231378206946705
8	0.020790514656527544
9	0.012937629044996839
10-11	0.01520514976532633
12-13	0.01120017810344567
14-15	0.01640271482108476
16-17	0.015450552440686664
18-19	0.05602052273125718
20-21	0.08449704918007041
22-23	0.10072307407489572
24-25	0.07120604028255467
26-27	0.08659769608115486
28-29	0.08856091748403754
30-31	0.0594168957582442
32-33	0.034101155768072086
34-35	0.4005953272582101
36-37	0.6272492382210152
38-39	0.5770791152703484
40-41	0.5717784174825652
42-43	0.5449215486911301
44-45	0.5294709962504435
46-47	0.5194683832027561
48-49	0.5100154721478761
50-51	0.5240034246434152
52-53	0.6039556162758122
54-55	0.5748312267640476
56-57	0.5315716431515279
58-59	0.510251058716222
60-61	0.5229825495139162
62-63	0.5351152577837311
64-65	0.5535302745427706
66-67	0.5800337634816868
68-69	0.533662473945598
70-71	0.4828346718249655
72-73	0.46610802547240504
74-75	0.4471531228275728
76-77	0.4613864779984722
78-79	0.4554281010407233
80-81	0.4525323494714713
82-83	0.47160504540047654
84-85	0.4395456398914024
86-87	0.44133217136802566
88-89	0.46815959183841743
90-91	0.4511875428104968
92-93	0.4464856275505927
94-95	0.45718518419630333
96-97	0.4517077964822606
98	0.44622059266120356
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	5093669.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.415389638271016
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.79219903694673	35.69016297797704
2	14.855490094960258	14.978906428097943
3	5.639590972008906	8.529665287629138
4	2.7805679792428566	5.607336723569141
5	1.6063202687386697	4.049163111615613
6	0.9641144175841512	2.9163722411027826
7	0.6518519870217364	2.3004360338527414
8	0.47103284494153136	1.899784352812048
9	0.3482186128790402	1.5800019342835565
>10	1.757656222337836	15.659301650604348
>50	0.09600333004439279	3.2989991358803676
>100	0.036133529837280284	3.0128841436857816
>500	6.252989594114825E-4	0.1995313222570409
>1k	1.5632359768645907E-4	0.1673357760825631
>5k	3.908089942042727E-5	0.11011888055002912
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5589	0.1097244442071128	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.88966420864803E-5
2	0.0	0.0	0.0	1.9632214028826764E-5	5.88966420864803E-5
3	0.0	0.0	0.0	1.9632214028826764E-5	5.88966420864803E-5
4	0.0	0.0	0.0	3.926442805765353E-5	5.88966420864803E-5
5	0.0	0.0	0.0	5.88966420864803E-5	5.88966420864803E-5
6	0.0	0.0	0.0	5.88966420864803E-5	5.88966420864803E-5
7	0.0	0.0	0.0	5.88966420864803E-5	5.88966420864803E-5
8	0.0	0.0	0.0	5.88966420864803E-5	5.88966420864803E-5
9	0.0	0.0	0.0	1.177932841729606E-4	5.88966420864803E-5
10-11	0.0	3.926442805765353E-5	0.0	1.3742549820178735E-4	5.88966420864803E-5
12-13	0.0	3.926442805765353E-5	0.0	1.3742549820178735E-4	3.141154244612282E-4
14-15	0.0	4.908053507206691E-5	0.0	1.3742549820178735E-4	3.239315314756416E-4
16-17	0.0	5.88966420864803E-5	0.0	1.570577122306141E-4	3.730120665477085E-4
18-19	0.0	5.88966420864803E-5	0.0	2.159543543170944E-4	4.122764946053621E-4
20-21	0.0	5.88966420864803E-5	0.0	2.552187823747479E-4	4.122764946053621E-4
22-23	0.0	5.88966420864803E-5	0.0	2.748509964035747E-4	4.122764946053621E-4
24-25	0.0	6.871274910089368E-5	0.0	3.33747638490055E-4	4.220926016197755E-4
26-27	0.0	7.852885611530706E-5	0.0	4.1227649460536203E-4	4.61357029677429E-4
28-29	0.0	7.852885611530706E-5	0.0	0.0011975650557584326	4.908053507206692E-4
30-31	0.0	7.852885611530706E-5	0.0	0.0038871783777076996	5.10437564749496E-4
32-33	0.0	7.852885611530706E-5	0.0	0.00949217548293774	5.88966420864803E-4
34-35	0.0	9.816107014413383E-5	0.0	0.016677565817488336	5.88966420864803E-4
36-37	0.0	1.177932841729606E-4	0.0	0.02710227146679535	5.88966420864803E-4
38-39	0.0	1.3742549820178735E-4	0.0	0.04465347080856648	5.88966420864803E-4
40-41	9.816107014413382E-6	1.4724160521620072E-4	0.0	0.063520028490269	5.88966420864803E-4
42-43	1.9632214028826764E-5	1.766899262594409E-4	0.0	0.08101233118995364	5.88966420864803E-4
44-45	1.9632214028826764E-5	2.159543543170944E-4	0.0	0.1001242915470165	6.184147419080431E-4
46-47	1.9632214028826764E-5	2.650348893891613E-4	0.0	0.12071848406325578	6.576791699656967E-4
48-49	1.9632214028826764E-5	3.141154244612282E-4	0.0	0.13985007663434745	7.067597050377636E-4
50-51	1.9632214028826764E-5	3.33747638490055E-4	0.0	0.1598945671577796	7.263919190665904E-4
52-53	1.9632214028826764E-5	3.533798525188818E-4	0.0	0.18064581738624946	7.852885611530706E-4
54-55	1.9632214028826764E-5	3.533798525188818E-4	0.0	0.2012007454744311	8.147368821963107E-4
56-57	1.9632214028826764E-5	3.533798525188818E-4	0.0	0.22220721448527575	8.343690962251375E-4
58-59	1.9632214028826764E-5	3.631959595332951E-4	0.0	0.24466646733425357	8.441852032395508E-4
60-61	1.9632214028826764E-5	3.730120665477085E-4	0.0	0.2685294234862925	8.441852032395508E-4
62-63	1.9632214028826764E-5	3.730120665477085E-4	0.0	0.29148929779300536	9.619784874125114E-4
64-65	1.9632214028826764E-5	3.828281735621219E-4	0.0	0.31505777073461194	9.816107014413383E-4
66-67	1.9632214028826764E-5	4.122764946053621E-4	0.0	0.33937226780931384	0.001020875129498992
68-69	1.9632214028826764E-5	4.220926016197755E-4	0.0	0.3649923071169328	0.001050323450542232
70-71	1.9632214028826764E-5	4.417248156486022E-4	0.0	0.3909460940630418	0.001099403985614299
72-73	1.9632214028826764E-5	4.515409226630156E-4	0.0	0.4159477186287527	0.0012270133768016729
74-75	1.9632214028826764E-5	4.515409226630156E-4	0.0	0.44268679413601475	0.0012760939118737398
76-77	1.9632214028826764E-5	4.515409226630156E-4	0.0	0.4698675944589254	0.0013251744469458067
78-79	1.9632214028826764E-5	4.515409226630156E-4	0.0	0.4980790860183495	0.0013742549820178734
80-81	1.9632214028826764E-5	4.61357029677429E-4	0.0	0.5272034755301139	0.0014331516241043538
82-83	1.9632214028826764E-5	4.711731366918424E-4	0.0	0.5562395200787487	0.001511680480219661
84-85	1.9632214028826764E-5	4.711731366918424E-4	0.0	0.5861295659376375	0.001511680480219661
86	1.9632214028826764E-5	4.711731366918424E-4	1.9632214028826764E-5	0.609246497956581	0.001511680480219661
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	9040	0.0	39.006847	1
GGTATCA	5295	0.0	31.906607	1
TCAACGC	11075	0.0	31.253334	4
CAACGCA	11160	0.0	31.015295	5
TATCAAC	11170	0.0	30.946486	2
ATCAACG	11455	0.0	30.016449	3
AACGCAG	12000	0.0	28.873917	6
GTACATG	17410	0.0	25.833088	1
TACATGG	18275	0.0	24.308577	2
ACGCAGA	14350	0.0	24.012257	7
ACATGGG	18160	0.0	23.427464	3
CGCAGAG	15490	0.0	22.034092	8
GAGTACA	13220	0.0	20.614405	1
CATGGGG	14020	0.0	20.077679	4
AGTACAT	12580	0.0	20.043573	2
GCAGAGT	17780	0.0	18.88661	9
GTATAGG	1050	0.0	17.975256	1
AGAGTAC	14290	0.0	17.664404	10-11
GTATATA	950	0.0	17.444536	1
GTACCTG	4240	0.0	16.828537	1
>>END_MODULE