FastQCFastQC Report
Mon 15 Aug 2016
SRR2921471_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921471_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5093669
Sequences flagged as poor quality0
Sequence length101
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA85350.039.6857531
GGTATCA52800.031.0379521
TCAACGC107500.030.5770824
CAACGCA109450.030.0588635
TATCAAC112800.029.2649972
ATCAACG113600.028.9343263
AACGCAG116950.028.2952336
GTACATG173800.026.3416021
TACATGG180600.025.1427612
ACATGGG182950.024.0416453
ACGCAGA140900.023.3520097
CGCAGAG152450.021.5205148
CATGGGG140550.021.4605734
GAGTACA133750.019.9107041
AGTACAT125650.019.1654172
GTACCTG46100.018.7045061
GCAGAGT173600.018.3233879
AGAGTAC141850.017.18658610-11
TACCTGG48800.016.5463392
ATGGGGG70800.016.4951745
Produced by FastQC (version 0.11.5)