##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921471_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5093669
Sequences flagged as poor quality	0
Sequence length	101
%GC	50
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.0409463198335	33.0	31.0	34.0	30.0	34.0
2	32.15847103531855	34.0	31.0	34.0	30.0	34.0
3	32.28300641443329	34.0	31.0	34.0	30.0	34.0
4	35.8051269134292	37.0	35.0	37.0	35.0	37.0
5	35.62145773508251	37.0	35.0	37.0	35.0	37.0
6	35.656565630785984	37.0	35.0	37.0	35.0	37.0
7	35.588216273966765	37.0	35.0	37.0	35.0	37.0
8	35.54205583440934	37.0	35.0	37.0	33.0	37.0
9	37.184517486314874	39.0	37.0	39.0	34.0	39.0
10-11	37.09915897558322	39.0	37.0	39.0	34.0	39.0
12-13	36.98613828264067	39.0	37.0	39.0	33.0	39.0
14-15	38.319270254898775	40.0	38.0	41.0	34.0	41.0
16-17	38.22978681967753	40.0	38.0	41.0	33.5	41.0
18-19	38.12002703748516	40.0	38.0	41.0	33.5	41.0
20-21	38.00478525400845	40.0	38.0	41.0	33.0	41.0
22-23	37.973962285338914	40.0	38.0	41.0	33.0	41.0
24-25	38.03524414326883	40.0	38.0	41.0	33.0	41.0
26-27	37.93697696100787	40.0	38.0	41.0	33.0	41.0
28-29	37.810546582434	40.0	38.0	41.0	33.0	41.0
30-31	37.678393806115004	40.0	38.0	41.0	33.0	41.0
32-33	37.52894917200156	40.0	38.0	41.0	32.0	41.0
34-35	37.39592943711105	40.0	37.0	41.0	32.0	41.0
36-37	37.315566441400094	40.0	37.0	41.0	32.0	41.0
38-39	37.29598497664453	40.0	37.0	41.0	32.0	41.0
40-41	37.140786631404595	40.0	37.0	41.0	31.5	41.0
42-43	36.9195612239429	40.0	36.5	41.0	31.0	41.0
44-45	36.729656167293165	39.5	36.0	41.0	31.0	41.0
46-47	36.48066403608087	39.0	36.0	41.0	30.0	41.0
48-49	36.17553054585997	39.0	35.0	40.0	30.0	41.0
50-51	35.90875751840177	39.0	35.0	40.0	29.0	41.0
52-53	35.60087061016333	38.0	35.0	40.0	28.0	41.0
54-55	35.276305645302045	38.0	34.5	40.0	28.0	41.0
56-57	34.899259453254615	38.0	34.0	40.0	27.0	41.0
58-59	34.5170608847964	37.0	34.0	40.0	26.0	41.0
60-61	34.095189636389804	37.0	33.0	39.5	26.0	41.0
62-63	33.648390089736885	36.0	33.0	39.0	25.5	40.0
64-65	33.14166831413662	35.5	32.0	39.0	24.0	40.0
66-67	32.619927600321105	35.0	32.0	38.0	22.5	40.0
68-69	32.12618262003283	35.0	31.0	37.5	22.0	39.5
70-71	31.58594757531359	35.0	31.0	37.0	20.0	39.0
72-73	31.052784544892887	34.0	30.0	36.0	18.5	39.0
74-75	30.547680463728604	34.0	30.0	35.5	18.0	38.0
76-77	29.242918120513917	32.5	28.5	34.5	17.0	36.0
78-79	30.17653139613116	34.0	30.0	35.0	17.5	37.0
80-81	30.228403435715983	34.0	30.0	35.0	17.5	36.5
82-83	29.91052382869794	34.0	30.0	35.0	13.0	36.0
84-85	29.452015236953955	34.0	29.5	35.0	7.0	35.5
86-87	28.95886599620038	34.0	29.0	35.0	4.0	35.0
88-89	28.43897021969822	33.0	29.0	35.0	2.0	35.0
90-91	27.997421603170523	33.0	27.0	35.0	2.0	35.0
92-93	27.589114644080723	33.0	27.0	35.0	2.0	35.0
94-95	27.121200749400877	33.0	26.5	34.0	2.0	35.0
96-97	26.391827776795076	32.0	25.0	34.0	2.0	35.0
98-99	25.422656438806683	32.0	22.0	34.0	2.0	35.0
100-101	21.817214860251028	28.0	2.0	32.5	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	29140.0
3	15834.0
4	9262.0
5	7053.0
6	6130.0
7	6255.0
8	7456.0
9	8853.0
10	10161.0
11	12252.0
12	14054.0
13	16101.0
14	18221.0
15	20167.0
16	22759.0
17	25967.0
18	26826.0
19	25493.0
20	26173.0
21	27323.0
22	30173.0
23	33584.0
24	38340.0
25	45029.0
26	54027.0
27	66161.0
28	79835.0
29	98617.0
30	121312.0
31	151634.0
32	197028.0
33	264594.0
34	381421.0
35	585538.0
36	928716.0
37	1165064.0
38	503617.0
39	13498.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.5949554808482	17.0130317729474	13.281359857727782	25.11065288847662
2	16.559124670252427	18.41717630258268	38.0396920176792	26.984007009485694
3	20.847802275148865	21.316488988355673	27.167808573519242	30.667900162976224
4	14.447587894369606	14.295191032354179	33.53477430020807	37.72244677306815
5	16.350694757334082	35.2097680857083	32.16460341103205	16.274933745925562
6	36.78834904384372	32.10774784891114	15.784421792776065	15.319481314469066
7	32.537228509983336	29.52214849280382	18.36394957583645	19.576673421376398
8	28.612242700006917	33.26677441873628	18.25684167992604	19.86414120133076
9	26.997984433150563	16.358947362008735	18.463276183357042	38.179792021483664
10-11	27.356042266001314	24.46965577565195	25.431889414614428	22.74241254373231
12-13	27.29406784798704	21.378314965292113	26.193282369932742	25.13433481678811
14-15	24.34820780105577	23.123479095874497	23.510832638713037	29.017480464356698
16-17	24.97456720052633	26.25476005773933	24.161071123462	24.609601618272336
18-19	24.99952864336096	25.507729659684436	25.480410614480153	24.01233108247445
20-21	25.446863362511362	24.71631124546357	25.485530005481866	24.351295386543203
22-23	25.245217471232944	24.321187406228535	25.447937651391996	24.985657471146524
24-25	24.979057521190175	24.558941233111614	25.482776793484817	24.97922445221339
26-27	24.900630521481126	24.79065744284365	25.500038290778203	24.808673744897018
28-29	24.940799673242083	24.894299507801247	25.44796173947536	24.71693907948131
30-31	25.139888442635872	24.81456351527224	25.234852183842104	24.810695858249783
32-33	24.939512473530726	24.811228950846345	25.200693327872674	25.048565247750254
34-35	25.061765541763343	24.75884515837375	25.42330269312193	24.756086606740975
36-37	25.041156030662947	24.75394490702404	25.343456144493032	24.86144291781998
38-39	25.035775688397287	24.844310958670636	25.305801630680225	24.814111722251848
40-41	25.190903699863725	24.74706536124824	25.325893155193203	24.736137783694836
42-43	25.155281637118147	24.681156543640927	25.204373742389052	24.95918807685187
44-45	24.982532063003365	24.722503523285592	25.278375150515842	25.0165892631952
46-47	25.03559988119463	24.739519231005254	25.329795355299233	24.895085532500875
48-49	25.099491423383334	24.870602987574724	25.11253738689449	24.91736820214745
50-51	24.941266184440085	25.040906536760517	25.08138113504792	24.936446143751482
52-53	25.099388136797966	24.8982706626167	25.038399125323537	24.963942075261794
54-55	25.147750989677633	24.77257317557652	25.142126120176073	24.937549714569776
56-57	25.018126124845562	24.89073770235931	25.131398453355143	24.959737719439982
58-59	25.103146585602854	24.894787096082624	25.071126013907058	24.93094030440746
60-61	25.192020989563236	24.817319550629215	25.116570236101172	24.87408922370638
62-63	25.06848247239446	24.928856965635944	25.077671719639994	24.9249888423296
64-65	25.082016763726834	24.787886325977848	25.105459240375406	25.024637669919912
66-67	25.08263046618529	24.89611903422175	25.01817192675452	25.003078572838444
68-69	24.977538829316025	24.87152662195092	25.128134508304424	25.022800040428635
70-71	24.950643301125243	24.743890028464406	25.230938300543077	25.07452836986727
72-73	25.129554055180627	24.823750023689183	24.99102265883855	25.055673262291645
74-75	24.908544741774428	25.043881238590192	24.97210771763739	25.075466301997988
76-77	25.09349765565392	24.815478562370714	24.999111374728404	25.09191240724697
78-79	24.944642022670124	24.81184771229432	25.183176676061553	25.060333588974004
80-81	24.963378016833445	24.911354081502594	25.15329778528078	24.97197011638318
82-83	25.11615110341216	24.862517174613668	25.019895696962095	25.00143602501207
84-85	25.023776911453083	24.96747027898341	24.92144316598989	25.08730964357362
86-87	25.010198847802922	24.913591115783927	25.11775480092763	24.958455235485523
88-89	24.95401581407971	24.765549483760346	25.0997323201796	25.180702381980336
90-91	24.987841112334756	24.8501812726001	25.08151467696676	25.080462938098385
92-93	25.096439400092375	24.940109313209263	25.077367090208785	24.886084196489573
94-95	25.025752294391257	24.876289344955676	24.983323468942288	25.11463489171078
96-97	24.98448718171481	24.794632471614804	25.07850158495306	25.14237876171732
98-99	24.939761922152297	24.828445174317373	25.055673099653575	25.176119803876755
100-101	25.473164620361455	24.384309499241695	25.10089018178934	25.04163569860751
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	203.0
1	183.5
2	290.0
3	433.5
4	368.5
5	330.5
6	698.5
7	1730.5
8	2555.0
9	2318.5
10	1578.0
11	1100.5
12	970.5
13	970.5
14	1055.0
15	1087.0
16	1143.0
17	1209.0
18	1283.5
19	1395.5
20	1373.5
21	1385.5
22	1471.5
23	1590.0
24	1728.0
25	1894.0
26	2190.5
27	2646.5
28	3329.5
29	4469.0
30	6362.0
31	9250.5
32	13673.5
33	19678.0
34	27129.5
35	36893.5
36	48899.5
37	62258.0
38	76413.0
39	90538.0
40	105561.5
41	120458.0
42	136008.5
43	151384.5
44	167368.0
45	183939.0
46	198552.5
47	212353.5
48	226551.5
49	235781.0
50	236896.5
51	238215.5
52	239995.0
53	241543.0
54	242059.0
55	234235.5
56	219920.5
57	203952.0
58	188308.5
59	169813.0
60	148180.0
61	127252.5
62	103976.5
63	79139.0
64	58905.5
65	44972.5
66	34669.0
67	26797.0
68	20411.0
69	15544.5
70	12180.0
71	9354.5
72	6972.0
73	5435.0
74	4138.5
75	3205.0
76	2346.0
77	1463.5
78	877.0
79	448.0
80	240.0
81	131.0
82	71.0
83	38.0
84	21.0
85	10.5
86	6.5
87	2.0
88	1.5
89	1.5
90	2.5
91	3.0
92	1.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.31566636936950554
2	0.0
3	0.005654077640302109
4	0.05915186086885505
5	3.926442805765353E-4
6	0.0655715948562814
7	0.07631041593004963
8	0.10689740538696174
9	0.09380271862973429
10-11	0.09894635870528688
12-13	0.097856770826687
14-15	0.06592497470880028
16-17	0.03571099731843589
18-19	0.03893068041916348
20-21	0.027966088884063725
22-23	0.04256264001449643
24-25	0.03426802958731712
26-27	0.020790514656527544
28-29	0.023833507830995693
30-31	0.0028663032482087077
32-33	0.005173088396595853
34-35	0.008176817143006348
36-37	0.010827166036897961
38-39	0.01619657657378208
40-41	0.020594192516239278
42-43	0.003573062953246471
44-45	0.015048092053095716
46-47	0.025649487628662166
48-49	0.0027386938570213336
50-51	0.006890907124118194
52-53	0.0025227395027042393
54-55	0.004270006551269821
56-57	0.003769385093534739
58-59	0.0013448066609746336
60-61	0.0055068360350859075
62-63	0.014920482661908341
64-65	0.006881091017103781
66-67	0.039735601194345375
68-69	0.06316664863775011
70-71	0.012603881406506782
72-73	0.03303120010350103
74-75	0.05212352824653506
76-77	0.30627235495671196
78-79	0.07543678240576684
80-81	0.034984605399369297
82-83	0.029202918367879815
84-85	0.01999540998836006
86-87	0.11945220625839645
88-89	0.5297065828187894
90-91	0.13457882716760747
92-93	0.049443731031600206
94-95	0.008510564781496404
96-97	0.005899480315662443
98-99	0.010699556645710588
100-101	0.007528954080055064
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	5093669.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.97261466512272
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.3490097940952	31.635590851356092
2	16.39742754137017	15.404600908001035
3	6.318177181262509	8.90343906364245
4	3.15306363340618	5.92430572266401
5	1.7639232202475343	4.1428042861774905
6	1.1290287976522804	3.1820060794768428
7	0.7269892568083952	2.390401035802128
8	0.5466967477254827	2.0543820535667923
9	0.40491949008629363	1.7118114460399303
>10	2.0502498130273157	17.033458675509063
>50	0.11503604652034471	3.690644492688633
>100	0.04460056070743057	3.46932410043024
>500	6.688897692009719E-4	0.2074667595869403
>1k	2.0902732164368893E-4	0.24976452505829244
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.926442805765353E-5
2	0.0	0.0	0.0	0.0	3.926442805765353E-5
3	0.0	0.0	0.0	0.0	3.926442805765353E-5
4	0.0	0.0	0.0	0.0	3.926442805765353E-5
5	0.0	0.0	0.0	0.0	3.926442805765353E-5
6	0.0	0.0	0.0	0.0	5.88966420864803E-5
7	0.0	0.0	0.0	1.9632214028826764E-5	5.88966420864803E-5
8	0.0	0.0	0.0	1.9632214028826764E-5	7.852885611530706E-5
9	0.0	0.0	0.0	1.9632214028826764E-5	7.852885611530706E-5
10-11	0.0	0.0	0.0	2.9448321043240146E-5	7.852885611530706E-5
12-13	0.0	0.0	0.0	3.926442805765353E-5	1.668738192450275E-4
14-15	0.0	0.0	0.0	5.88966420864803E-5	2.0613824730268102E-4
16-17	0.0	0.0	0.0	1.079771771585472E-4	2.257704613315078E-4
18-19	0.0	0.0	0.0	2.355865683459212E-4	2.748509964035747E-4
20-21	0.0	0.0	0.0	2.552187823747479E-4	3.631959595332951E-4
22-23	0.0	0.0	0.0	2.552187823747479E-4	4.220926016197755E-4
24-25	0.0	0.0	0.0	3.239315314756416E-4	4.711731366918424E-4
26-27	0.0	0.0	0.0	4.515409226630156E-4	4.711731366918424E-4
28-29	0.0	0.0	0.0	0.0012270133768016729	4.908053507206692E-4
30-31	0.0	0.0	0.0	0.0038871783777076996	4.908053507206692E-4
32-33	0.0	0.0	0.0	0.00943327884085126	5.202536717639093E-4
34-35	0.0	0.0	0.0	0.016579404747344203	5.693342068359762E-4
36-37	0.0	1.9632214028826764E-5	0.0	0.026827420470391775	5.693342068359762E-4
38-39	0.0	1.9632214028826764E-5	0.0	0.0441528493508314	5.693342068359762E-4
40-41	0.0	1.9632214028826764E-5	0.0	0.06273473992911593	5.693342068359762E-4
42-43	0.0	5.88966420864803E-5	0.0	0.08006016880955555	5.791503138503895E-4
44-45	0.0	9.816107014413383E-5	0.0	0.0990936003105031	5.987825278792164E-4
46-47	0.0	9.816107014413383E-5	0.0	0.11958963175659824	6.478630629512833E-4
48-49	0.0	1.27609391187374E-4	0.0	0.13847582165232958	7.165758120521769E-4
50-51	0.0	1.3742549820178735E-4	0.0	0.15826509339338696	7.46024133095417E-4
52-53	0.0	1.3742549820178735E-4	0.0	0.178829837588583	8.343690962251375E-4
54-55	0.0	1.3742549820178735E-4	0.0	0.1989921213961881	9.030818453260312E-4
56-57	0.0	1.3742549820178735E-4	0.0	0.21956668169839855	9.128979523404445E-4
58-59	0.0	1.3742549820178735E-4	0.0	0.24138788759143948	9.22714059354858E-4
60-61	0.0	1.3742549820178735E-4	0.0	0.26459316457351273	9.22714059354858E-4
62-63	0.0	1.570577122306141E-4	0.0	0.28684627917518785	0.0010405073435278186
64-65	0.0	1.766899262594409E-4	0.0	0.3100122917292034	0.0011092200926287122
66-67	0.0	1.8650603327385429E-4	0.0	0.3338261673461703	0.0011975650557584326
68-69	0.0	1.9632214028826765E-4	0.0	0.3583566187751894	0.0012466455908304995
70-71	0.0	2.0613824730268102E-4	0.0	0.3833386111268714	0.0012859100188881531
72-73	0.0	2.159543543170944E-4	0.0	0.407525498810386	0.001492048266190834
74-75	0.0	2.159543543170944E-4	0.0	0.4335970790406679	0.0016000254433493812
76-77	0.0	2.159543543170944E-4	0.0	0.46030670622688674	0.0016196576573782081
78-79	0.0	2.159543543170944E-4	0.0	0.4878507025093307	0.0016491059784214483
80-81	0.0	2.355865683459212E-4	0.0	0.5157775269653367	0.001688370406479102
82-83	0.0	2.355865683459212E-4	0.0	0.5438712252405878	0.001796347583637649
84-85	0.0	2.355865683459212E-4	0.0	0.5728778214681794	0.0018356120116953026
86-87	0.0	2.355865683459212E-4	0.0	0.6026893384709529	0.0019043247607961962
88-89	0.0	2.355865683459212E-4	0.0	0.6344149963415369	0.0019730375098970902
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	8535	0.0	39.685753	1
GGTATCA	5280	0.0	31.037952	1
TCAACGC	10750	0.0	30.577082	4
CAACGCA	10945	0.0	30.058863	5
TATCAAC	11280	0.0	29.264997	2
ATCAACG	11360	0.0	28.934326	3
AACGCAG	11695	0.0	28.295233	6
GTACATG	17380	0.0	26.341602	1
TACATGG	18060	0.0	25.142761	2
ACATGGG	18295	0.0	24.041645	3
ACGCAGA	14090	0.0	23.352009	7
CGCAGAG	15245	0.0	21.520514	8
CATGGGG	14055	0.0	21.460573	4
GAGTACA	13375	0.0	19.910704	1
AGTACAT	12565	0.0	19.165417	2
GTACCTG	4610	0.0	18.704506	1
GCAGAGT	17360	0.0	18.323387	9
AGAGTAC	14185	0.0	17.186586	10-11
TACCTGG	4880	0.0	16.546339	2
ATGGGGG	7080	0.0	16.495174	5
>>END_MODULE