FastQCFastQC Report
Mon 15 Aug 2016
SRR2921470_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921470_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4010423
Sequences flagged as poor quality0
Sequence length98
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53750.13402576236970512No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA71400.042.356721
GGTATCA41150.035.6842041
TCAACGC92900.031.9770744
TATCAAC95050.031.3984572
ATCAACG95050.031.3502253
CAACGCA94850.031.3196645
AACGCAG101150.029.542516
GTACATG139550.026.685411
TACATGG145700.024.85692
ACGCAGA119050.024.7491667
ACATGGG147500.023.5900693
CGCAGAG125950.023.353448
GTACCTG39700.022.0301911
TACCTGG40900.020.3998782
CATGGGG119400.020.1957914
GTGTAGC18450.019.9594611
GCAGAGT144900.019.7296499
GAGTACA110800.019.069011
GTATAGG9200.019.0130121
AGTACAT100700.018.9839252
Produced by FastQC (version 0.11.5)