##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921470_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4010423
Sequences flagged as poor quality	0
Sequence length	98
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.593013505059194	31.0	31.0	34.0	27.0	34.0
2	30.697216228811772	31.0	31.0	34.0	27.0	34.0
3	30.83117042765813	31.0	31.0	34.0	27.0	34.0
4	34.341693133118376	37.0	35.0	37.0	31.0	37.0
5	34.331508172579305	37.0	35.0	37.0	31.0	37.0
6	34.43188561406116	37.0	35.0	37.0	32.0	37.0
7	34.32511533072696	37.0	35.0	37.0	31.0	37.0
8	34.30418337417275	37.0	35.0	37.0	31.0	37.0
9	35.79159729534765	39.0	37.0	39.0	30.0	39.0
10-11	35.674355174005335	39.0	37.0	39.0	30.0	39.0
12-13	35.52429681357802	39.0	36.5	39.0	30.0	39.0
14-15	36.57940134993241	40.0	37.0	41.0	30.0	41.0
16-17	36.44034108122759	40.0	36.5	41.0	30.0	41.0
18-19	36.262572052873224	40.0	36.0	41.0	29.0	41.0
20-21	36.150936696702566	39.0	36.0	41.0	29.0	41.0
22-23	36.203881984518844	40.0	36.5	41.0	29.0	41.0
24-25	36.08085481257214	40.0	36.0	41.0	28.5	41.0
26-27	35.82519873838745	39.0	36.0	41.0	27.0	41.0
28-29	35.59676385757812	39.0	36.0	40.5	27.0	41.0
30-31	35.349214534227436	39.0	35.0	40.0	26.0	41.0
32-33	35.093126710075225	39.0	35.0	40.0	25.0	41.0
34-35	34.67910729117602	38.0	34.5	40.0	24.0	41.0
36-37	34.311525492448055	38.0	34.0	40.0	22.5	41.0
38-39	34.08646008164226	38.0	33.5	40.0	21.0	41.0
40-41	33.82532353819036	38.0	33.0	40.0	18.5	41.0
42-43	33.54816960704644	38.0	33.0	40.0	17.5	41.0
44-45	33.23902952381831	38.0	33.0	40.0	15.0	41.0
46-47	32.95601025128771	38.0	32.0	40.0	14.0	41.0
48-49	32.75264516984866	37.0	32.0	40.0	10.5	41.0
50-51	31.990290051697787	36.0	31.0	39.0	10.5	40.0
52-53	32.48104227409428	37.0	31.5	39.0	11.5	40.0
54-55	32.91262779512286	37.0	33.0	40.0	8.5	41.0
56-57	32.76406391545231	37.0	32.5	40.0	8.0	41.0
58-59	32.43895531718225	37.0	32.0	40.0	6.5	41.0
60-61	31.986808623429496	36.0	31.0	39.0	2.0	41.0
62-63	31.501732734925966	35.5	31.0	39.0	2.0	40.0
64-65	30.96273211080228	35.0	30.0	38.5	2.0	40.0
66-67	30.47925717561464	35.0	30.0	38.0	2.0	40.0
68-69	29.96693802623813	34.5	29.0	37.0	2.0	39.5
70-71	29.45469019103471	34.0	29.0	36.5	2.0	39.0
72-73	28.904404099018983	34.0	28.0	36.0	2.0	39.0
74-75	28.313321687014064	34.0	27.0	35.5	2.0	38.0
76-77	27.730746607028735	33.0	26.0	35.0	2.0	37.0
78-79	27.17634810592299	33.0	25.5	35.0	2.0	36.5
80-81	26.61034758677576	32.5	24.5	35.0	2.0	36.0
82-83	25.98985705497899	32.0	23.5	34.5	2.0	35.5
84-85	25.382382107822544	31.5	20.5	34.0	2.0	35.0
86-87	24.766175538091616	31.0	18.5	34.0	2.0	35.0
88-89	24.11965346298882	31.0	13.0	34.0	2.0	35.0
90-91	23.38683699948858	30.5	4.5	34.0	2.0	35.0
92-93	22.571773351589094	30.0	2.0	34.0	2.0	35.0
94-95	21.667186478832782	29.0	2.0	34.0	2.0	35.0
96-97	20.765409908131886	29.0	2.0	33.5	2.0	34.5
98	20.138854430068847	27.0	2.0	33.0	2.0	34.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	110581.0
3	19659.0
4	15064.0
5	12908.0
6	12290.0
7	12492.0
8	14420.0
9	16874.0
10	18732.0
11	20514.0
12	23083.0
13	26740.0
14	22183.0
15	22135.0
16	24397.0
17	27626.0
18	31608.0
19	31274.0
20	32524.0
21	35761.0
22	39076.0
23	42848.0
24	49064.0
25	54719.0
26	62301.0
27	71922.0
28	83232.0
29	97720.0
30	116679.0
31	141668.0
32	177663.0
33	230487.0
34	314585.0
35	445852.0
36	609123.0
37	647997.0
38	282541.0
39	12080.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.073382772961864	16.810116751014682	13.291301297790248	24.825199178233202
2	16.216931579912412	18.34521004504128	38.87630050305036	26.56155787199595
3	20.635241801444337	21.604129129091667	27.035386903766923	30.72524216569707
4	14.003220295345093	14.255395267053995	34.053475663668245	37.68790877393267
5	16.0464453053559	35.41231224255513	32.229346213238514	16.311896238850448
6	36.10955099282989	32.47352033627695	16.09716042393411	15.31976824695906
7	32.106517912178674	29.931277881176516	18.51982399514263	19.44238021150218
8	28.304309245327158	33.53799750502412	18.427257387038996	19.73043586260973
9	27.058764265126236	16.614055921838506	18.53861665246035	37.78856316057491
10-11	27.09749017417154	24.719315483909583	25.466789198571515	22.716405143347366
12-13	27.046138745413955	21.730654325494914	26.308045003581054	24.915161925510077
14-15	24.143766245182945	23.337737080695813	23.77437517807744	28.744121496043796
16-17	24.62858623485335	26.51475193403495	24.389402079513083	24.467259751598615
18-19	24.66339637939906	25.688827904510937	26.004284845555333	23.64349087053467
20-21	25.151236481925366	24.905637069876608	25.786503324694575	24.15662312350345
22-23	24.90783378795051	24.571616338463308	25.664245050742117	24.856304822844066
24-25	24.57172171898677	24.89167816319101	25.82680782377469	24.709792294047535
26-27	24.697150058645903	24.983691447680368	25.794726859823815	24.524431633849915
28-29	24.658007216818245	25.15436000088844	25.71105674553391	24.476576036759404
30-31	24.80523260129258	25.06470208350252	25.61261929826728	24.51744601693762
32-33	24.66374161248133	25.038184465984305	25.534248912458267	24.763825009076097
34-35	24.72255730323408	25.08738636017988	25.711786547088124	24.478269789497915
36-37	24.82208091125085	25.101213711418662	25.51442409868672	24.562281278643763
38-39	24.863535813988133	25.016417333443847	25.566512426701955	24.55353442586607
40-41	24.89813825762159	24.99992790213649	25.57747567449432	24.524458165747596
42-43	24.863113500969988	24.873680837845658	25.56537132593879	24.697834335245567
44-45	24.78831953213155	25.098509735916576	25.488024106324715	24.62514662562716
46-47	24.857331590370695	25.047969695408884	25.46144083658196	24.63325787763846
48-49	24.869891326740056	25.12782804352544	25.313047758391587	24.68923287134291
50-51	24.817617503706554	25.233691476868973	25.191443657249994	24.75724736217448
52-53	24.871649568327133	25.280130806311135	25.318925674401054	24.529293950960678
54-55	24.789466134267187	25.022846498115854	25.409556707716803	24.778130659900157
56-57	24.79828467871003	25.121091861611667	25.402374672664997	24.678248787013313
58-59	24.827824899677303	25.117941196814936	25.463770048249128	24.590463855258633
60-61	24.90667603770506	24.95963878055854	25.400723431464385	24.73296175027202
62-63	24.746814936348862	25.161103524517582	25.412248668411824	24.67983287072173
64-65	24.80450263851548	24.95446188299769	25.46031420485872	24.78072127362811
66-67	24.77603671542001	25.20443260523713	25.32564194490204	24.69388873444082
68-69	24.802934851245737	25.14478601150166	25.317428743824205	24.734850393428395
70-71	24.681429300348977	25.00432511267529	25.437763413768526	24.8764821732072
72-73	24.83691558940543	25.028166591910644	25.276408364040503	24.858509454643425
74-75	24.703433023851087	25.179177446783996	25.281564559231995	24.83582497013292
76-77	24.82147587458504	24.99845006678803	25.333911975065803	24.846162083561126
78-79	24.79864179411223	24.86401722504841	25.428938597316275	24.908402383523086
80-81	24.654929152365685	25.145543791379758	25.327938471726945	24.87158858452761
82-83	24.882327012221555	25.140814893190864	25.180234420299342	24.796623674288234
84-85	24.822212279908925	25.072160032676905	25.214007041511916	24.891620645902258
86-87	24.8146012866399	25.0223209757566	25.348050693221257	24.81502704438224
88-89	24.814992911104284	24.821656560757642	25.328419601501224	25.03493092663685
90-91	24.785861632976932	24.866287189618966	25.309642150104995	25.038209027299104
92-93	24.765043855474797	25.127546547915426	25.325334488786922	24.782075107822855
94-95	24.822445436734693	24.922402265304033	25.313850790167436	24.94130150779384
96-97	24.843161589836907	24.984990550997686	25.22960011120893	24.942247747956472
98	24.8619037755777	24.94167461661783	25.263087239245152	24.93333436855932
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	601.0
1	467.5
2	524.5
3	699.5
4	559.0
5	520.5
6	938.5
7	2141.5
8	3206.5
9	3007.0
10	2264.0
11	1831.5
12	1895.0
13	2185.0
14	2474.0
15	2707.0
16	2939.0
17	3089.0
18	3105.5
19	3073.5
20	2894.0
21	2621.5
22	2475.5
23	2423.5
24	2471.0
25	2682.0
26	3083.5
27	3852.5
28	5044.0
29	6831.0
30	9704.0
31	13772.5
32	19500.5
33	26135.0
34	33725.5
35	43365.5
36	53739.0
37	64187.0
38	74533.5
39	84349.0
40	95116.0
41	107505.0
42	120673.0
43	134935.5
44	147682.5
45	158685.0
46	170074.0
47	182719.0
48	189294.5
49	188566.0
50	188270.0
51	186380.5
52	185021.0
53	185217.0
54	182379.0
55	174096.0
56	161551.0
57	150907.5
58	140925.5
59	128538.0
60	112761.5
61	93948.0
62	75729.5
63	58334.5
64	43696.5
65	33262.0
66	25574.0
67	19597.0
68	14988.5
69	11670.5
70	8909.5
71	6773.5
72	5497.0
73	4194.5
74	3172.5
75	2424.0
76	1647.0
77	1039.0
78	626.5
79	361.0
80	204.0
81	116.5
82	82.5
83	64.5
84	34.5
85	21.5
86	20.0
87	17.5
88	11.0
89	8.5
90	9.0
91	8.0
92	8.0
93	5.5
94	6.0
95	7.5
96	3.5
97	2.0
98	2.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.474338991173749
2	0.04682797799633605
3	0.05528095166021141
4	0.05223887854223856
5	0.06298587455737213
6	0.06323522481294366
7	0.04164149268044792
8	0.01907529455122315
9	0.011694526986305435
10-11	0.014537119899821042
12-13	0.010746996015133566
14-15	0.014474782335928155
16-17	0.01351478385197771
18-19	0.05262537143837445
20-21	0.08198636403192382
22-23	0.09820659815685279
24-25	0.06707521874874545
26-27	0.08285908992642423
28-29	0.08462947674098219
30-31	0.05596666486303315
32-33	0.0315802098681361
34-35	0.39770119012383476
36-37	0.6244727800533759
38-39	0.574041690864031
40-41	0.5682068948836569
42-43	0.5415139500247231
44-45	0.5260417666665087
46-47	0.515269835625818
48-49	0.5061062137335638
50-51	0.5203441133266989
52-53	0.6002110001862646
54-55	0.57203442130668
56-57	0.5286100742988956
58-59	0.506156083784678
60-61	0.5192968422532984
62-63	0.5310038367523825
64-65	0.5487201724107408
66-67	0.5761611680363892
68-69	0.5301186433451035
70-71	0.4786901531334724
72-73	0.46250732154687924
74-75	0.4421728082050198
76-77	0.45669746059206223
78-79	0.4512616250206026
80-81	0.44838162956875127
82-83	0.4674444566071958
84-85	0.43358269190058
86-87	0.4374102183236033
88-89	0.46420290328476577
90-91	0.4466112427541933
92-93	0.4429208589717344
94-95	0.4538049976274323
96-97	0.4472844884442364
98	0.44224761328169127
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	4010423.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.339529217130476
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.69024390435071	39.30602917753585
2	14.052407170721914	14.990975657074706
3	5.104666916494127	8.168415904081733
4	2.356811584867416	5.0284488156122835
5	1.3134625703378418	3.502973757307129
6	0.7605482109733653	2.434037011215008
7	0.5323498507552914	1.987670329266784
8	0.36740020848414334	1.5677563323855832
9	0.2757152001875636	1.3235867078410375
>10	1.4152725960107133	13.589579666277343
>50	0.08870332734330402	3.2393672505083217
>100	0.040491476303363805	3.7794917734569173
>500	0.0014569873176586887	0.5239245514561779
>1k	4.2299626738635186E-4	0.4229952693410583
>5k	4.6999585265149585E-5	0.13474779664008604
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5375	0.13402576236970512	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.4935025557154443E-5
2	0.0	0.0	0.0	0.0	2.4935025557154443E-5
3	0.0	0.0	0.0	0.0	2.4935025557154443E-5
4	0.0	2.4935025557154443E-5	0.0	2.4935025557154443E-5	2.4935025557154443E-5
5	0.0	4.987005111430889E-5	0.0	2.4935025557154443E-5	2.4935025557154443E-5
6	0.0	4.987005111430889E-5	0.0	2.4935025557154443E-5	2.4935025557154443E-5
7	0.0	4.987005111430889E-5	0.0	2.4935025557154443E-5	2.4935025557154443E-5
8	0.0	4.987005111430889E-5	0.0	2.4935025557154443E-5	2.4935025557154443E-5
9	0.0	4.987005111430889E-5	0.0	2.4935025557154443E-5	2.4935025557154443E-5
10-11	0.0	4.987005111430889E-5	0.0	4.987005111430889E-5	2.4935025557154443E-5
12-13	0.0	4.987005111430889E-5	0.0	4.987005111430889E-5	9.974010222861777E-5
14-15	0.0	6.233756389288612E-5	0.0	4.987005111430889E-5	1.7454517890008113E-4
16-17	0.0	7.480507667146334E-5	0.0	4.987005111430889E-5	3.116878194644306E-4
18-19	0.0	7.480507667146334E-5	0.0	4.987005111430889E-5	3.6155787057873945E-4
20-21	0.0	7.480507667146334E-5	0.0	6.233756389288612E-5	4.987005111430889E-4
22-23	0.0	7.480507667146334E-5	0.0	1.4961015334292665E-4	5.485705622573978E-4
24-25	0.0	8.727258945004056E-5	0.0	2.4935025557154447E-4	5.485705622573978E-4
26-27	0.0	9.974010222861777E-5	0.0	4.2389543447162554E-4	5.735055878145522E-4
28-29	0.0	9.974010222861777E-5	0.0	8.477908689432512E-4	5.735055878145522E-4
30-31	0.0	9.974010222861777E-5	0.0	0.003029605605194265	5.735055878145522E-4
32-33	0.0	9.974010222861777E-5	0.0	0.007343365026581984	6.483106644860156E-4
34-35	0.0	9.974010222861777E-5	0.0	0.013477381313641977	7.480507667146333E-4
36-37	0.0	1.4961015334292668E-4	0.0	0.023401521485389445	7.480507667146333E-4
38-39	0.0	1.9948020445723557E-4	0.0	0.03730279823350305	8.103883306075194E-4
40-41	1.2467512778577222E-5	2.4935025557154447E-4	0.0	0.05319887702618901	8.228558433860967E-4
42-43	2.4935025557154443E-5	2.4935025557154447E-4	0.0	0.0681474248477031	8.353233561646739E-4
44-45	2.4935025557154443E-5	2.4935025557154447E-4	0.0	0.08495363207322519	9.101284328361372E-4
46-47	2.4935025557154443E-5	2.4935025557154447E-4	0.0	0.10194685199042594	9.849335095076005E-4
48-49	2.4935025557154443E-5	2.4935025557154447E-4	0.0	0.11900240947151958	0.0010846736117362185
50-51	2.4935025557154443E-5	2.4935025557154447E-4	0.0	0.13708030300045654	0.0010971411245147957
52-53	2.4935025557154443E-5	2.4935025557154447E-4	0.0	0.1545098858649075	0.0011968812267434134
54-55	2.4935025557154443E-5	2.4935025557154447E-4	0.0	0.17321115503277335	0.0011968812267434134
56-57	2.4935025557154443E-5	2.4935025557154447E-4	0.0	0.19177528156007484	0.0012592187906362994
58-59	2.4935025557154443E-5	2.4935025557154447E-4	0.0	0.2106760309323979	0.0013215563545291855
60-61	2.4935025557154443E-5	2.618177683501217E-4	0.0	0.22875392446133488	0.0014212964567578033
62-63	2.4935025557154443E-5	2.742852811286989E-4	0.0	0.24826558195980825	0.0014711665078721123
64-65	2.4935025557154443E-5	2.742852811286989E-4	0.0	0.2679891871755174	0.001521036558986421
66-67	2.4935025557154443E-5	2.742852811286989E-4	0.0	0.2892213614374344	0.0015584390973221527
68-69	4.987005111430889E-5	2.742852811286989E-4	0.0	0.31128885905551607	0.0015958416356578844
70-71	4.987005111430889E-5	2.742852811286989E-4	0.0	0.334441030285334	0.0016457116867721934
72-73	4.987005111430889E-5	2.9922030668585336E-4	0.0	0.35761813654070906	0.0018202568656722744
74-75	4.987005111430889E-5	2.9922030668585336E-4	0.0	0.37956095903100495	0.0019199969679008922
76-77	4.987005111430889E-5	3.7402538335731665E-4	0.0	0.40268819523526567	0.001957399506236624
78-79	4.987005111430889E-5	3.7402538335731665E-4	0.0	0.4265634822062411	0.001969867019015201
80-81	4.987005111430889E-5	3.7402538335731665E-4	0.0	0.4509624047139167	0.0019823345317937784
82-83	4.987005111430889E-5	3.7402538335731665E-4	0.0	0.4765457409355571	0.0022192172745867456
84-85	4.987005111430889E-5	3.7402538335731665E-4	0.0	0.503238685794491	0.0022192172745867456
86	4.987005111430889E-5	3.7402538335731665E-4	0.0	0.5229124209590859	0.0022441523001439
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	7140	0.0	42.35672	1
GGTATCA	4115	0.0	35.684204	1
TCAACGC	9290	0.0	31.977074	4
TATCAAC	9505	0.0	31.398457	2
ATCAACG	9505	0.0	31.350225	3
CAACGCA	9485	0.0	31.319664	5
AACGCAG	10115	0.0	29.54251	6
GTACATG	13955	0.0	26.68541	1
TACATGG	14570	0.0	24.8569	2
ACGCAGA	11905	0.0	24.749166	7
ACATGGG	14750	0.0	23.590069	3
CGCAGAG	12595	0.0	23.35344	8
GTACCTG	3970	0.0	22.030191	1
TACCTGG	4090	0.0	20.399878	2
CATGGGG	11940	0.0	20.195791	4
GTGTAGC	1845	0.0	19.959461	1
GCAGAGT	14490	0.0	19.729649	9
GAGTACA	11080	0.0	19.06901	1
GTATAGG	920	0.0	19.013012	1
AGTACAT	10070	0.0	18.983925	2
>>END_MODULE