FastQCFastQC Report
Mon 15 Aug 2016
SRR2921470_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921470_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4010423
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49970.12460032270910075No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA72600.042.1921231
GGTATCA43100.033.490561
TCAACGC93600.031.96784
TATCAAC96350.031.3503632
ATCAACG97150.030.7996523
CAACGCA97100.030.751245
AACGCAG104050.028.8804286
GTACATG141650.026.0304341
ACGCAGA123300.024.2569247
TACATGG146800.024.0057372
ACATGGG149400.022.6666323
CGCAGAG131550.022.483068
GTACCTG38300.021.5181221
CATGGGG118900.020.2522894
GAGTACA115250.020.1713941
AGTACAT102450.019.7948912
GCAGAGT149950.019.3438179
TACCTGG41900.018.9295232
GTATAGG10250.018.5905881
GTGTAGC21350.017.8504491
Produced by FastQC (version 0.11.5)