##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921470_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4010423
Sequences flagged as poor quality	0
Sequence length	101
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.97823346814039	33.0	31.0	34.0	30.0	34.0
2	32.0927735054382	34.0	31.0	34.0	30.0	34.0
3	32.21803061672048	34.0	31.0	34.0	30.0	34.0
4	35.751423976972006	37.0	35.0	37.0	35.0	37.0
5	35.55718361878535	37.0	35.0	37.0	33.0	37.0
6	35.59318705283707	37.0	35.0	37.0	35.0	37.0
7	35.524594288432915	37.0	35.0	37.0	33.0	37.0
8	35.474498575337314	37.0	35.0	37.0	33.0	37.0
9	37.101774301613574	39.0	37.0	39.0	34.0	39.0
10-11	37.01748369186991	39.0	37.0	39.0	33.5	39.0
12-13	36.90049528441264	39.0	37.0	39.0	33.0	39.0
14-15	38.21699805731216	40.0	38.0	41.0	33.0	41.0
16-17	38.12776333070103	40.0	38.0	41.0	33.0	41.0
18-19	38.02070429977087	40.0	38.0	41.0	33.0	41.0
20-21	37.90928463157128	40.0	38.0	41.0	33.0	41.0
22-23	37.8778388214909	40.0	38.0	41.0	33.0	41.0
24-25	37.935033287012374	40.0	38.0	41.0	33.0	41.0
26-27	37.831736328063144	40.0	38.0	41.0	33.0	41.0
28-29	37.70064192729794	40.0	38.0	41.0	33.0	41.0
30-31	37.5609484336191	40.0	38.0	41.0	32.0	41.0
32-33	37.40595717708581	40.0	37.0	41.0	32.0	41.0
34-35	37.27094099550097	40.0	37.0	41.0	32.0	41.0
36-37	37.18115021781992	40.0	37.0	41.0	31.5	41.0
38-39	37.15342957588264	40.0	37.0	41.0	31.0	41.0
40-41	36.99089460139243	40.0	37.0	41.0	31.0	41.0
42-43	36.76235249997319	40.0	36.0	41.0	30.5	41.0
44-45	36.567376433857476	39.0	36.0	41.0	30.0	41.0
46-47	36.3073375302306	39.0	35.5	41.0	30.0	41.0
48-49	35.99339895068425	39.0	35.0	40.0	29.0	41.0
50-51	35.725106428922835	39.0	35.0	40.0	28.5	41.0
52-53	35.414426109166044	38.0	35.0	40.0	28.0	41.0
54-55	35.09430987703791	38.0	34.0	40.0	27.5	41.0
56-57	34.72060740226156	38.0	34.0	40.0	26.5	41.0
58-59	34.33699063664855	37.0	34.0	40.0	26.0	41.0
60-61	33.91988613171229	37.0	33.0	39.5	25.5	41.0
62-63	33.48376655031153	36.0	33.0	39.0	24.5	40.0
64-65	32.9771045348583	35.5	32.0	39.0	23.0	40.0
66-67	32.451588149180274	35.0	31.5	38.0	22.0	40.0
68-69	31.956080942085162	35.0	31.0	37.5	20.5	39.5
70-71	31.410535995828866	35.0	31.0	37.0	19.0	39.0
72-73	30.864426395918834	34.0	30.0	36.0	18.0	39.0
74-75	30.350941534097526	34.0	29.5	36.0	14.5	38.0
76-77	29.05115146207769	32.5	28.0	34.5	12.5	36.0
78-79	29.98952591783959	34.0	30.0	35.0	13.0	37.0
80-81	30.030883899279452	34.0	30.0	35.0	12.0	36.5
82-83	29.70943264588299	34.0	30.0	35.0	7.5	36.0
84-85	29.231845867630422	34.0	29.0	35.0	4.5	36.0
86-87	28.721339868637298	34.0	29.0	35.0	2.0	35.0
88-89	28.193774696584377	33.0	28.0	35.0	2.0	35.0
90-91	27.738775435907883	33.0	27.0	35.0	2.0	35.0
92-93	27.31504369987904	33.0	26.0	34.5	2.0	35.0
94-95	26.834592011865084	32.5	25.5	34.0	2.0	35.0
96-97	26.085213205689275	32.0	24.5	34.0	2.0	35.0
98-99	25.091215939066778	32.0	20.5	34.0	2.0	35.0
100-101	21.504656117322288	28.0	2.0	32.5	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	25372.0
3	13258.0
4	7747.0
5	6016.0
6	5096.0
7	5296.0
8	6160.0
9	7281.0
10	8587.0
11	10039.0
12	11959.0
13	13952.0
14	15644.0
15	17268.0
16	19634.0
17	22555.0
18	23288.0
19	21669.0
20	21535.0
21	22741.0
22	24513.0
23	27465.0
24	31294.0
25	36742.0
26	43687.0
27	53003.0
28	64328.0
29	79062.0
30	97429.0
31	121722.0
32	156981.0
33	211682.0
34	302678.0
35	460813.0
36	720292.0
37	895197.0
38	387324.0
39	11114.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.24565077529114	17.233831049867373	13.668402320920602	24.852115853920882
2	16.287434018805495	18.655438590891784	38.405524803742644	26.65160258656007
3	20.574686703979992	21.832883021606236	27.281110472459503	30.311319801954273
4	14.20524970110914	14.558105580826275	34.083562699218994	37.15308201884559
5	16.091607796826302	35.40017569315388	32.33903542314098	16.169181086878844
6	36.08564255369689	32.636121697480945	15.945360192465943	15.332875556356221
7	32.08684175525112	29.94120099885686	18.62795177199273	19.344005473899287
8	28.32693521698139	33.67806362604081	18.3677152521625	19.6272859048153
9	26.972039074263716	16.61155380822822	18.541930656385034	37.87447646112304
10-11	27.03573285384356	24.818625122645035	25.535800619046988	22.60984140446442
12-13	27.05061151719737	21.70691677548026	26.409498437464705	24.832973269857664
14-15	24.072526380414175	23.3578495494548	23.85804223835511	28.711581831775916
16-17	24.558307368532382	26.56955569197244	24.53498457078444	24.337152368710733
18-19	24.646665993988048	25.791620711034604	26.02956933892613	23.532143956051222
20-21	25.087781457189024	24.88887874566954	25.94711998875599	24.076219808385442
22-23	24.847999692669138	24.583812394464154	25.853348297419444	24.714839615447268
24-25	24.50503989763059	24.96753562600243	25.92974325332189	24.59768122304509
26-27	24.60932833429229	25.020232847969442	25.94522004734175	24.42521877039652
28-29	24.588341807053578	25.201579765828523	25.872568715698268	24.33750971141963
30-31	24.75163199368423	25.047035380830113	25.74126388712606	24.4600687383596
32-33	24.523446105016784	24.971890408376446	25.776875799368458	24.727787687238308
34-35	24.719834527218936	25.012858204644267	25.885881984311805	24.381425283824996
36-37	24.642259573608	25.196985881904354	25.66784675942903	24.492907785058613
38-39	24.755039368272154	25.037994148377436	25.73831096994684	24.46865551340357
40-41	24.755861012893263	25.033154369362432	25.722603143289845	24.488381474454457
42-43	24.78442890991597	24.95941677858742	25.7184664005837	24.537687910912908
44-45	24.706416772094524	25.00331059682766	25.65069126009919	24.639581370978622
46-47	24.724603027430643	25.09258463683016	25.65020621959753	24.532606116141668
48-49	24.7435198434631	25.181068102003827	25.53068030687476	24.54473174765831
50-51	24.754298345098487	25.241312789453158	25.36113380354042	24.64325506190794
52-53	24.783979451039436	25.305844950950977	25.44593336524264	24.46424223276695
54-55	24.81771436928567	25.004173671098755	25.51920281448191	24.658909145133663
56-57	24.727206851004908	25.20148877644988	25.471520268071522	24.599784104473684
58-59	24.769472872106192	25.171355589279205	25.497472305575492	24.561699233039114
60-61	24.901311191830146	25.09268537667574	25.409813684158163	24.596189747335952
62-63	24.661963074364756	25.19083664871667	25.526672740054913	24.620527536863662
64-65	24.81747920500419	24.984243145440974	25.500930823325106	24.69734682622973
66-67	24.75438414219135	25.270379808281753	25.386460436361887	24.58877561316502
68-69	24.85312047034384	25.198196140357137	25.343581768437645	24.605101620861376
70-71	24.679830356754316	25.07973614098649	25.506325586301752	24.73410791595744
72-73	24.944555003942853	25.063956767931185	25.262814207537826	24.728674020588137
74-75	24.68952604533414	25.201794505092185	25.26877971533157	24.839899734242106
76-77	24.771522263284833	25.042745591917498	25.439420183072308	24.746311961725358
78-79	24.72695472419233	24.998814691043606	25.509071481366696	24.76515910339737
80-81	24.652833077890982	25.2620284787563	25.381632620227418	24.703505823125305
82-83	24.850017721267044	25.213222634235795	25.221852554568656	24.71490708992851
84-85	24.745042540607454	25.215970163901268	25.308634354784264	24.730352940707014
86-87	24.74426717718572	25.173591498794252	25.403345044864107	24.678796279155925
88-89	24.736899036638665	25.01691168609103	25.359532356313963	24.88665692095634
90-91	24.696341061789447	25.07388761591873	25.40818897252457	24.821582349767258
92-93	24.733976104095685	25.347234046268767	25.380464034836603	24.538325814798945
94-95	24.74399538762779	25.14994698365421	25.328796105019247	24.777261523698755
96-97	24.69236548728155	25.197614980012204	25.328207068131565	24.78181246457468
98-99	24.76748600851241	25.13785753339597	25.350649091558598	24.744007366533022
100-101	25.18286863806286	24.661163421833614	25.43434496582271	24.721622974280812
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	334.0
1	267.0
2	362.0
3	533.0
4	416.0
5	372.0
6	757.0
7	1911.5
8	2843.5
9	2584.5
10	1777.5
11	1163.5
12	1048.0
13	1092.5
14	1075.5
15	1105.0
16	1187.0
17	1258.0
18	1358.0
19	1402.5
20	1396.0
21	1459.5
22	1582.0
23	1716.0
24	1886.0
25	2117.5
26	2474.0
27	3050.5
28	3958.0
29	5380.5
30	7768.0
31	11185.5
32	15913.5
33	21708.0
34	28227.5
35	36161.0
36	46051.0
37	56550.0
38	66862.0
39	77159.5
40	87557.5
41	97781.5
42	109161.5
43	123793.0
44	139043.0
45	153107.0
46	162707.0
47	172009.5
48	184382.0
49	189558.5
50	186913.0
51	183626.5
52	182282.0
53	180709.0
54	178513.5
55	172591.5
56	159821.5
57	147006.0
58	137231.5
59	126770.5
60	110916.0
61	93367.5
62	76786.0
63	58490.0
64	43043.0
65	32560.0
66	25059.5
67	19562.0
68	14975.0
69	11297.5
70	8771.5
71	6928.0
72	5335.5
73	4161.5
74	3126.5
75	2252.5
76	1620.0
77	989.5
78	595.0
79	338.5
80	183.5
81	96.0
82	57.5
83	26.5
84	13.5
85	7.0
86	4.5
87	2.5
88	1.0
89	0.5
90	1.0
91	1.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3166498895503043
2	0.0
3	0.005934536082602758
4	0.056627443040297744
5	5.485705622573977E-4
6	0.06605288270090212
7	0.07585234774486382
8	0.10712086979353551
9	0.09465335701495828
10-11	0.09829387074630283
12-13	0.09858062354021009
14-15	0.06651418067370948
16-17	0.036654487569017034
18-19	0.039260197739739676
20-21	0.0293360575679922
22-23	0.04273863380496272
24-25	0.03523319111225923
26-27	0.021294511825809895
28-29	0.024137104739325503
30-31	0.0035906436802302403
32-33	0.005635315775916905
34-35	0.008415571125539626
36-37	0.010659723425683526
38-39	0.014836340206506895
40-41	0.0192124371917875
42-43	0.0036155787057873946
44-45	0.014262834618692342
46-47	0.024523597635461396
48-49	0.0030545406307514195
50-51	0.006857132028217473
52-53	0.002456100017379713
54-55	0.004101811704151906
56-57	0.003740253833573167
58-59	0.0012218162523005677
60-61	0.005398433033123938
62-63	0.014462314823149578
64-65	0.006570379234310197
66-67	0.03918539266306821
68-69	0.061377565408935665
70-71	0.011931409729098402
72-73	0.03165501494480757
74-75	0.05175264554387404
76-77	0.30065407065538974
78-79	0.07552819241262082
80-81	0.03402384237273724
82-83	0.02782748852178436
84-85	0.01953659252403051
86-87	0.12034890085160592
88-89	0.5315524073146399
90-91	0.13350212683300489
92-93	0.04975784349930169
94-95	0.008452973663875357
96-97	0.005635315775916905
98-99	0.009861802607854582
100-101	0.007580247769374951
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4010423.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.66371109841134
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.27935837977641	34.903337507549296
2	15.647480749622074	15.542239267343675
3	5.801552941670387	8.643799476517696
4	2.7257999962874346	5.414933741106793
5	1.4580556600694754	3.620622753354694
6	0.917295598403031	2.733378215455949
7	0.6213636874519756	2.160145866246093
8	0.42485259053917906	1.687980505275955
9	0.3166458175818745	1.4153225764415827
>10	1.6480449014387066	14.768457950838746
>50	0.10781721876448956	3.6572240904529276
>100	0.04941707627300443	4.262356739072604
>500	0.001711369433711655	0.5670030057489339
>1k	6.040126883340317E-4	0.6231983045951457
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4997	0.12460032270910075	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.4961015334292668E-4
2	0.0	0.0	0.0	0.0	1.4961015334292668E-4
3	0.0	0.0	0.0	0.0	1.4961015334292668E-4
4	0.0	0.0	0.0	0.0	1.4961015334292668E-4
5	0.0	0.0	0.0	0.0	1.4961015334292668E-4
6	0.0	0.0	0.0	0.0	1.4961015334292668E-4
7	0.0	0.0	0.0	0.0	1.4961015334292668E-4
8	0.0	0.0	0.0	2.4935025557154443E-5	1.7454517890008113E-4
9	0.0	0.0	0.0	2.4935025557154443E-5	1.7454517890008113E-4
10-11	0.0	0.0	0.0	7.480507667146334E-5	1.7454517890008113E-4
12-13	0.0	0.0	0.0	8.727258945004056E-5	5.735055878145522E-4
14-15	0.0	0.0	0.0	1.2467512778577223E-4	6.607781772645929E-4
16-17	0.0	0.0	0.0	1.2467512778577223E-4	7.480507667146333E-4
18-19	0.0	0.0	0.0	1.2467512778577223E-4	7.480507667146333E-4
20-21	0.0	0.0	0.0	1.3714264056434946E-4	7.979208178289423E-4
22-23	0.0	0.0	0.0	2.742852811286989E-4	8.9766092005756E-4
24-25	0.0	0.0	0.0	3.4909035780016225E-4	0.0010472710734004867
26-27	0.0	0.0	0.0	5.361030494788206E-4	0.00112207615007195
28-29	0.0	0.0	0.0	9.974010222861779E-4	0.00112207615007195
30-31	0.0	0.0	0.0	0.003229085809651501	0.0011594786884076818
32-33	0.0	0.0	0.0	0.007492975179924911	0.0012218162523005677
34-35	0.0	2.4935025557154443E-5	0.0	0.013626991466984904	0.0012218162523005677
36-37	0.0	3.740253833573166E-5	0.0	0.02368827427929672	0.0012218162523005677
38-39	0.0	6.233756389288612E-5	0.0	0.03760201854018891	0.0013215563545291855
40-41	0.0	9.974010222861779E-5	0.0	0.05346069479453913	0.00134649138008634
42-43	0.0	1.4961015334292668E-4	0.0	0.0684217101288318	0.0013838939184220717
44-45	0.0	1.4961015334292668E-4	0.0	0.08515311227768244	0.0014462314823149578
46-47	0.0	1.620776661215039E-4	0.0	0.10202165706709741	0.001508569046207844
48-49	0.0	1.7454517890008113E-4	0.0	0.11874059170316947	0.0015459715845435756
50-51	0.0	1.7454517890008113E-4	0.0	0.13640705731041342	0.0016457116867721934
52-53	0.0	1.7454517890008113E-4	0.0	0.15346261479150702	0.0017080492506650795
54-55	0.0	1.7454517890008113E-4	0.0	0.1719893387804728	0.0017454517890008112
56-57	0.0	1.7454517890008113E-4	0.0	0.19022930997553128	0.001807789352893697
58-59	0.0	1.7454517890008113E-4	0.0	0.20898044919451142	0.0018202568656722744
60-61	0.0	2.2441523001439E-4	0.0	0.2268214599806554	0.0018202568656722744
62-63	0.0	2.2441523001439E-4	0.0	0.24579701442964993	0.0019199969679008922
64-65	0.0	2.2441523001439E-4	0.0	0.2653710094920162	0.0019324644806794694
66-67	0.0	2.2441523001439E-4	0.0	0.28622915837057583	0.001957399506236624
68-69	0.0	2.2441523001439E-4	0.0	0.3079475656308574	0.001969867019015201
70-71	0.0	2.2441523001439E-4	0.0	0.3306384388878679	0.0020072695573509327
72-73	0.0	2.2441523001439E-4	0.0	0.3534539872726643	0.0022316847873653225
74-75	0.0	2.2441523001439E-4	0.0	0.3752970696607315	0.002368827427929672
76-77	0.0	2.4935025557154447E-4	0.0	0.3982871632244279	0.002368827427929672
78-79	0.0	2.4935025557154447E-4	0.0	0.42172608724815314	0.002393762453486827
80-81	0.0	2.4935025557154447E-4	0.0	0.4457260493469143	0.002418697479043981
82-83	0.0	2.4935025557154447E-4	0.0	0.4707358799807402	0.0025433726068297535
84-85	0.0	2.4935025557154447E-4	0.0	0.4969425918413095	0.0025558401196083304
86-87	0.0	2.4935025557154447E-4	0.0	0.5231493037018788	0.0027303852985084117
88-89	0.0	2.4935025557154447E-4	0.0	0.5512884800431276	0.0028675279390727613
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	7260	0.0	42.192123	1
GGTATCA	4310	0.0	33.49056	1
TCAACGC	9360	0.0	31.9678	4
TATCAAC	9635	0.0	31.350363	2
ATCAACG	9715	0.0	30.799652	3
CAACGCA	9710	0.0	30.75124	5
AACGCAG	10405	0.0	28.880428	6
GTACATG	14165	0.0	26.030434	1
ACGCAGA	12330	0.0	24.256924	7
TACATGG	14680	0.0	24.005737	2
ACATGGG	14940	0.0	22.666632	3
CGCAGAG	13155	0.0	22.48306	8
GTACCTG	3830	0.0	21.518122	1
CATGGGG	11890	0.0	20.252289	4
GAGTACA	11525	0.0	20.171394	1
AGTACAT	10245	0.0	19.794891	2
GCAGAGT	14995	0.0	19.343817	9
TACCTGG	4190	0.0	18.929523	2
GTATAGG	1025	0.0	18.590588	1
GTGTAGC	2135	0.0	17.850449	1
>>END_MODULE