FastQCFastQC Report
Mon 15 Aug 2016
SRR2921469_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921469_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4457262
Sequences flagged as poor quality0
Sequence length98
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT206930.46425361578475755No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT88020.19747549055900235No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC75920.1703287803140134No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT74590.16734488571683692No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT62890.1410955873807732No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52560.11791992483277851No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC47520.10661253478032029No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG55950.035.130921
TACCTGG59100.032.6563722
ACCTGGG69800.026.3367563
GTACATG124950.025.3831041
TACATGG129150.024.0307662
ACATGGG132350.022.86093
GTACACG10900.020.6933781
CATGGGG104950.019.6563744
GTGTAGC23900.019.452941
GAGTACA99750.019.2435381
AGTACAT90350.018.7735712
CCTGGGG77400.017.8870754
AGAGTAC128900.017.8579810-11
TACACGG10450.017.5475712
GTATAGG8450.016.8875541
GTACCGG7650.016.2466621
GTATTAG9200.016.0112021
GTATAGC10550.015.7076731
AGTACTT79600.015.42861212-13
GAGTACT68950.015.25293912-13
Produced by FastQC (version 0.11.5)