##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2921469_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4457262 Sequences flagged as poor quality 0 Sequence length 98 %GC 49 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.032655024541974 31.0 31.0 34.0 26.0 34.0 2 30.120154480486 31.0 31.0 34.0 26.0 34.0 3 30.257710675297975 31.0 31.0 34.0 26.0 34.0 4 33.73194104362723 37.0 35.0 37.0 28.0 37.0 5 33.71050501406469 37.0 35.0 37.0 28.0 37.0 6 33.805915380338874 37.0 35.0 37.0 30.0 37.0 7 33.68313709178415 37.0 35.0 37.0 29.0 37.0 8 33.65805689681244 37.0 35.0 37.0 29.0 37.0 9 35.073113494338 39.0 35.0 39.0 29.0 39.0 10-11 34.9367242715371 39.0 35.0 39.0 28.0 39.0 12-13 34.76862971034684 39.0 35.0 39.0 27.0 39.0 14-15 35.73192589980127 39.5 36.0 41.0 27.0 41.0 16-17 35.584281785544576 39.0 36.0 41.0 27.0 41.0 18-19 35.396168208195974 39.0 36.0 41.0 26.5 41.0 20-21 35.27020107859937 39.0 36.0 40.0 26.0 41.0 22-23 35.30592872934102 39.0 36.0 41.0 25.5 41.0 24-25 35.15920737887968 39.0 35.5 41.0 25.0 41.0 26-27 34.8701229588927 39.0 35.0 40.0 24.0 41.0 28-29 34.625987097011574 39.0 34.0 40.0 22.5 41.0 30-31 34.36598196381546 38.5 34.0 40.0 20.0 41.0 32-33 34.07308859115753 38.0 34.0 40.0 18.5 41.0 34-35 33.629484423397145 38.0 33.0 40.0 15.5 41.0 36-37 33.227701669769466 38.0 33.0 40.0 9.5 41.0 38-39 32.97437630994094 38.0 32.5 40.0 9.0 41.0 40-41 32.70856458516461 38.0 32.0 40.0 8.5 41.0 42-43 32.391033778135544 38.0 31.0 40.0 8.0 41.0 44-45 32.05732162480015 37.0 31.0 40.0 4.5 41.0 46-47 31.74098280513912 37.0 30.0 40.0 2.0 41.0 48-49 31.498430650924266 37.0 30.0 40.0 2.0 41.0 50-51 30.759851451406714 36.0 29.0 39.0 2.0 40.0 52-53 31.29414167262324 36.5 30.5 39.0 2.0 40.0 54-55 31.715429674091403 37.0 31.0 40.0 2.0 40.5 56-57 31.534366389052295 37.0 31.0 40.0 2.0 41.0 58-59 31.192724255383688 36.0 30.5 39.0 2.0 41.0 60-61 30.705535371266038 35.5 29.5 39.0 2.0 40.0 62-63 30.224848909487484 35.0 29.0 39.0 2.0 40.0 64-65 29.677161450235594 35.0 28.0 38.0 2.0 40.0 66-67 29.162200023242967 34.5 28.0 38.0 2.0 40.0 68-69 28.62619204345627 34.0 26.5 37.0 2.0 39.0 70-71 28.07182716654305 34.0 26.0 36.0 2.0 39.0 72-73 27.507619139283264 33.5 25.5 36.0 2.0 39.0 74-75 26.901801711454254 33.0 24.5 35.0 2.0 37.5 76-77 26.313992648401644 32.5 22.5 35.0 2.0 37.0 78-79 25.724100131425974 32.0 20.5 35.0 2.0 36.5 80-81 25.10279931940281 31.5 18.0 35.0 2.0 36.0 82-83 24.447356920010535 31.0 12.5 34.0 2.0 35.5 84-85 23.805936245165753 31.0 7.0 34.0 2.0 35.0 86-87 23.181713908673082 30.0 2.0 34.0 2.0 35.0 88-89 22.49753402425076 30.0 2.0 34.0 2.0 35.0 90-91 21.72166769644683 29.0 2.0 34.0 2.0 35.0 92-93 20.88539444169986 29.0 2.0 34.0 2.0 35.0 94-95 19.961525371405138 27.5 2.0 33.0 2.0 34.5 96-97 19.06942602880423 26.0 2.0 33.0 2.0 34.0 98 18.456976278262307 25.0 2.0 33.0 2.0 34.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 176432.0 3 29066.0 4 21878.0 5 18146.0 6 17199.0 7 17845.0 8 19902.0 9 23305.0 10 25989.0 11 27998.0 12 30733.0 13 34829.0 14 29828.0 15 30074.0 16 33369.0 17 38269.0 18 42651.0 19 41222.0 20 43292.0 21 46602.0 22 50498.0 23 55994.0 24 62079.0 25 69037.0 26 78138.0 27 88698.0 28 100803.0 29 117185.0 30 138425.0 31 166237.0 32 204317.0 33 259523.0 34 343484.0 35 466116.0 36 608053.0 37 620168.0 38 267200.0 39 12678.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.831598476557176 16.797478812580238 12.862834915650456 24.508087795212134 2 16.06422214186486 18.873407751432683 38.62744688726333 26.43492321943912 3 21.426180707532207 21.94773951152518 27.2037341042305 29.42234567671211 4 13.730584893788626 14.175449056229366 34.87824374506576 37.21572230491624 5 15.414304819347164 35.680891144478494 32.60919687574997 16.295607160424368 6 35.263873114426325 32.97474946012611 16.38841375297158 15.372963672475985 7 31.668310411435975 29.97348760143596 18.948329552795183 19.409872434332883 8 28.12465218062552 33.087092176173435 18.472844058437246 20.315411584763808 9 26.583832674588066 17.152223447591886 19.081608497757873 37.18233538006218 10-11 27.031296455237484 24.646135204725603 25.701429909740103 22.62113843029681 12-13 27.406229841084638 21.71535471276876 26.284499441860987 24.59391600428561 14-15 23.931307486241334 23.499482952697438 24.43652951253496 28.132680048526264 16-17 24.302702889489098 26.618223170528392 24.776348611868105 24.30272532811441 18-19 24.216316940757963 26.28139331259966 26.013387281734406 23.48890246490797 20-21 24.918158790023266 24.99154024943858 25.86809839851926 24.222202562018897 22-23 24.47129234259596 24.84065275494829 25.90613059905042 24.78192430340533 24-25 24.60896543889403 25.007139518311515 25.974903470569245 24.408991572225208 26-27 24.638700741819903 24.82902843059147 26.44191861807188 24.090352209516745 28-29 24.14063262247409 25.782991766029813 25.91527511107164 24.16110050042446 30-31 24.55430121551357 25.133099027562206 25.769511724791887 24.54308803213234 32-33 24.390736827552743 25.2630741468767 25.883511132461894 24.462677893108662 34-35 24.828848895063967 25.08586838799897 25.977327321645138 24.107955395291924 36-37 24.679922225864686 24.994344397092412 26.14347034116042 24.182263035882485 38-39 24.469636495440326 25.32631917432254 26.054095526076033 24.14994880416111 40-41 24.599446628686135 25.368218471103027 25.830113382000402 24.202221518210436 42-43 24.39553409311109 25.313712295816593 25.60164612246933 24.689107488602996 44-45 24.62717209701493 24.9810342387391 25.680548577666585 24.711245086579392 46-47 24.973187795488197 25.341787245002152 25.62563408002311 24.059390879486543 48-49 24.798539469644464 25.2861082857609 25.56713996121594 24.348212283378697 50-51 24.104693975360153 25.555129542443122 26.000857038143838 24.339319444052887 52-53 24.545478554470115 25.81580685456984 25.34276460818292 24.295949982777124 54-55 24.30195810744487 25.213981485137904 25.705082278873764 24.778978128543464 56-57 24.568445334078262 25.20537507940899 25.680616672769773 24.545562913742973 58-59 25.09770180195711 25.193675690564937 25.423767225435697 24.28485528204225 60-61 24.685051308244944 25.25002331449717 25.988847607537906 24.076077769719976 62-63 24.290453139857 25.47621069603187 25.8468734172033 24.386462746907828 64-65 24.28052552908067 25.70700414786295 25.656784447956117 24.355685875100267 66-67 24.55118346029431 25.31237387445379 25.39250749517895 24.743935170072948 68-69 24.31979851261856 25.39594070413668 25.59279735843294 24.69146342481181 70-71 24.69394513646449 25.132388567038323 25.583997586909796 24.58966870958739 72-73 24.71561325386042 24.963056996360248 25.875102894943026 24.44622685483631 74-75 24.238421460820526 25.847410598600163 25.53316441478874 24.381003525790575 76-77 24.470923798511986 25.191117902808113 25.45608424108421 24.88187405759569 78-79 24.519239708360974 25.16768703063661 25.699525620622033 24.613547640380382 80-81 24.74128315980372 25.22093389347801 25.50672306576216 24.53105988095611 82-83 24.666416084024455 25.104424774771473 25.755650366209437 24.47350877499463 84-85 24.515897010419692 25.345150474993844 25.648518301780122 24.490434212806345 86-87 24.505455813515905 25.426507777963685 25.59945782694742 24.468578581572988 88-89 24.32074040597455 25.338956403549208 25.308481827392633 25.031821363083612 90-91 24.56072958800553 24.93811473968977 25.44847657690224 25.052679095402464 92-93 24.857309264275244 25.34989723337438 25.529019106896563 24.26377439545382 94-95 24.752903021147866 25.096673463718787 25.56948459788741 24.580938917245938 96-97 24.2937656389762 25.108913644567977 25.989417724702633 24.60790299175319 98 24.65451143035107 25.48894824184155 25.145059677961655 24.711480649845726 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 636.0 1 476.0 2 568.5 3 804.0 4 618.5 5 527.5 6 853.5 7 1607.0 8 2379.5 9 2405.0 10 1943.5 11 1669.5 12 1786.0 13 2162.5 14 2527.5 15 2931.5 16 3323.5 17 3406.5 18 3412.0 19 3440.5 20 3392.0 21 3305.0 22 3343.5 23 3648.5 24 4133.5 25 4980.5 26 6326.0 27 8389.0 28 11087.0 29 14448.5 30 18778.0 31 24012.0 32 30281.5 33 37172.5 34 44960.5 35 53805.5 36 63369.5 37 73714.0 38 84640.5 39 95841.5 40 107222.0 41 118764.5 42 131116.5 43 144538.5 44 157203.5 45 172204.0 46 199191.5 47 226508.5 48 230101.5 49 214314.0 50 204398.0 51 200682.5 52 196986.0 53 195932.0 54 192164.5 55 184365.0 56 172494.5 57 160429.0 58 147828.5 59 132374.5 60 115315.5 61 97242.5 62 79115.0 63 61240.5 64 45999.0 65 35228.5 66 26945.0 67 20552.5 68 15796.5 69 12158.5 70 9234.0 71 7008.5 72 5572.0 73 4296.5 74 3249.5 75 2472.5 76 1740.0 77 1081.5 78 668.0 79 399.0 80 245.5 81 148.0 82 92.0 83 54.5 84 40.5 85 30.5 86 19.5 87 16.0 88 14.0 89 11.0 90 10.0 91 10.5 92 8.5 93 4.0 94 3.0 95 6.5 96 9.0 97 8.0 98 4.5 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4769071237006036 2 0.048527548975133165 3 0.05696322091903056 4 0.054270985192254796 5 0.0660719517946219 6 0.06687962251265464 7 0.04460137187358518 8 0.020775085691619653 9 0.012496460831784177 10-11 0.015413049535791255 12-13 0.01092598999116498 14-15 0.015850537841392315 16-17 0.014829731794989838 18-19 0.05634625023164445 20-21 0.08572527260008499 22-23 0.10241713410609472 24-25 0.07134424675955778 26-27 0.08727330814298105 28-29 0.08845116127344545 30-31 0.06021633908888461 32-33 0.0343596584629757 34-35 0.4018161822212829 36-37 0.6287716539884799 38-39 0.5776976987217713 40-41 0.5725039272988665 42-43 0.5451552993743692 44-45 0.5297983380828859 46-47 0.5200614188710468 48-49 0.5094719583457289 50-51 0.5246943078508735 52-53 0.6081760506786453 54-55 0.5762954926140756 56-57 0.5323335267255997 58-59 0.5097860525138527 60-61 0.5233145370409009 62-63 0.535115503643268 64-65 0.5550156127236855 66-67 0.5813770875483649 68-69 0.5347004506353901 70-71 0.48137174794750676 72-73 0.4643657922733732 74-75 0.444611512628156 76-77 0.4592954149879455 78-79 0.4532266669538385 80-81 0.45038860179186235 82-83 0.4696717401848937 84-85 0.43558130529459566 86-87 0.43893538230420376 88-89 0.4661381807935006 90-91 0.4491097898216439 92-93 0.44378140661240023 94-95 0.455200973153474 96-97 0.44940144869204457 98 0.4437253183680923 >>END_MODULE >>Sequence Length Distribution pass #Length Count 98 4457262.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.832286888046134 #Duplication Level Percentage of deduplicated Percentage of total 1 76.68021848570238 43.57912175617496 2 13.002031881694624 14.778704120559825 3 4.406078715090856 7.512225887620717 4 1.9781722880525396 4.496962199543383 5 1.0588083012459935 3.008724856792853 6 0.6380623461060615 2.1757525383815683 7 0.4240492860031003 1.6869783473759523 8 0.3160472655027776 1.4369351090589075 9 0.22378113828093346 1.1446194465823982 >10 1.1714442351136025 11.95651783793042 >50 0.07035525767125972 2.7172135876363113 >100 0.02900723154877165 2.7359280134308372 >500 8.726227094199869E-4 0.32575927998665444 >1k 8.329574381282792E-4 1.1801356385823887 >5k 1.9832319955424556E-4 0.7979531122885507 >10k+ 3.9664639910849114E-5 0.46646826805432456 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 20693 0.46425361578475755 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 8802 0.19747549055900235 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 7592 0.1703287803140134 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 7459 0.16734488571683692 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 6289 0.1410955873807732 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5256 0.11791992483277851 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 4752 0.10661253478032029 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 4.487059544626275E-5 0.0 0.0 0.0 6 0.0 6.730589316939412E-5 0.0 0.0 0.0 7 0.0 6.730589316939412E-5 0.0 2.2435297723131374E-5 0.0 8 0.0 6.730589316939412E-5 0.0 2.2435297723131374E-5 0.0 9 0.0 6.730589316939412E-5 0.0 2.2435297723131374E-5 0.0 10-11 0.0 6.730589316939412E-5 0.0 6.730589316939412E-5 0.0 12-13 0.0 7.852354203095981E-5 0.0 6.730589316939412E-5 8.97411908925255E-5 14-15 0.0 1.0095883975409119E-4 0.0 8.97411908925255E-5 1.0095883975409119E-4 16-17 0.0 1.1217648861565688E-4 0.0 1.4582943520035393E-4 1.3461178633878825E-4 18-19 0.0 1.1217648861565688E-4 0.0 2.1313532836974807E-4 1.79482381785051E-4 20-21 0.0 1.1217648861565688E-4 0.0 2.2435297723131376E-4 2.2435297723131376E-4 22-23 0.0 1.1217648861565688E-4 0.0 2.692235726775765E-4 2.2435297723131376E-4 24-25 0.0 1.1217648861565688E-4 0.0 3.5896476357010203E-4 2.692235726775765E-4 26-27 0.0 1.1217648861565688E-4 0.0 4.2627065673949614E-4 3.028765192622736E-4 28-29 0.0 1.2339413747722256E-4 0.0 8.637589623405579E-4 3.365294658469706E-4 30-31 0.0 1.3461178633878825E-4 0.0 0.0028941534062839475 3.365294658469706E-4 32-33 0.0 1.3461178633878825E-4 0.0 0.0069773775918938585 3.58964763570102E-4 34-35 0.0 1.9070003064661667E-4 0.0 0.012204801961383467 4.0383535901636477E-4 36-37 0.0 2.0191767950818239E-4 0.0 0.01924948544644672 4.487059544626275E-4 38-39 0.0 2.2435297723131376E-4 0.0 0.029782857727456903 4.8235890104732454E-4 40-41 0.0 2.467882749544451E-4 0.0 0.04076493596292971 4.935765499088903E-4 42-43 0.0 2.467882749544451E-4 0.0 0.05153387887003277 4.935765499088903E-4 44-45 2.2435297723131374E-5 2.467882749544451E-4 0.0 0.0637835514268625 5.272294964935873E-4 46-47 2.2435297723131374E-5 2.467882749544451E-4 0.0 0.07703159473237159 5.945353896629814E-4 48-49 2.2435297723131374E-5 2.692235726775765E-4 0.0 0.08981971443455647 6.506236339708099E-4 50-51 2.2435297723131374E-5 2.9165887040070787E-4 0.0 0.10332576366388155 6.506236339708099E-4 52-53 2.2435297723131374E-5 2.9165887040070787E-4 0.0 0.11684303054206821 6.954942294170726E-4 54-55 2.2435297723131374E-5 3.1409416812383924E-4 0.0 0.13065195629065557 7.17929527140204E-4 56-57 2.2435297723131374E-5 3.1409416812383924E-4 0.0 0.14642397059001694 7.17929527140204E-4 58-59 2.2435297723131374E-5 3.253118169854049E-4 0.0 0.1613097906293146 7.17929527140204E-4 60-61 2.2435297723131374E-5 3.365294658469706E-4 0.0 0.17656579308104392 7.628001225864668E-4 62-63 2.2435297723131374E-5 3.365294658469706E-4 0.0 0.1918666661282195 8.188883668942951E-4 64-65 2.2435297723131374E-5 3.365294658469706E-4 0.0 0.20694318619816382 8.301060157558609E-4 66-67 2.2435297723131374E-5 3.365294658469706E-4 0.0 0.22363504770417356 8.413236646174266E-4 68-69 4.487059544626275E-5 3.701824124316677E-4 0.0 0.2399006385534438 8.525413134789923E-4 70-71 4.487059544626275E-5 3.814000612932334E-4 0.0 0.25718703544911653 8.749766112021236E-4 72-73 4.487059544626275E-5 3.814000612932334E-4 0.0 0.27344140864952515 9.983707486793461E-4 74-75 4.487059544626275E-5 3.814000612932334E-4 0.0 0.2911989467973837 0.0010993295884334374 76-77 4.487059544626275E-5 4.0383535901636477E-4 0.0 0.30842925544874855 0.0011666354816028315 78-79 4.487059544626275E-5 4.0383535901636477E-4 0.0 0.32772361149064155 0.0012002884281875287 80-81 4.487059544626275E-5 4.2627065673949614E-4 0.0 0.34854356777770745 0.0012339413747722255 82-83 4.487059544626275E-5 4.935765499088903E-4 0.0 0.367075123697014 0.0013461178633878824 84-85 4.487059544626275E-5 5.160118476320217E-4 0.0 0.3869079268842621 0.0013461178633878824 86 6.730589316939412E-5 5.160118476320217E-4 0.0 0.40233219406891496 0.0013461178633878824 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACCTG 5595 0.0 35.13092 1 TACCTGG 5910 0.0 32.656372 2 ACCTGGG 6980 0.0 26.336756 3 GTACATG 12495 0.0 25.383104 1 TACATGG 12915 0.0 24.030766 2 ACATGGG 13235 0.0 22.8609 3 GTACACG 1090 0.0 20.693378 1 CATGGGG 10495 0.0 19.656374 4 GTGTAGC 2390 0.0 19.45294 1 GAGTACA 9975 0.0 19.243538 1 AGTACAT 9035 0.0 18.773571 2 CCTGGGG 7740 0.0 17.887075 4 AGAGTAC 12890 0.0 17.85798 10-11 TACACGG 1045 0.0 17.547571 2 GTATAGG 845 0.0 16.887554 1 GTACCGG 765 0.0 16.246662 1 GTATTAG 920 0.0 16.011202 1 GTATAGC 1055 0.0 15.707673 1 AGTACTT 7960 0.0 15.428612 12-13 GAGTACT 6895 0.0 15.252939 12-13 >>END_MODULE