FastQCFastQC Report
Mon 15 Aug 2016
SRR2921469_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921469_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4457262
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT209230.46941373426107774No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT86550.19417750179370205No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC74340.16678400327375864No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT73510.16492187356273874No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT63470.14239683464871483No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56950.1277690205332332No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC48990.10991052354562061No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG45430.10192355755618585No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT44790.10048769850190542No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG56400.036.572151
TACCTGG56400.035.4586942
TATCACG4300.028.722662
ACCTGGG69600.027.7100013
GTACATG131200.024.326671
TACATGG136450.022.3501912
TATAACG4200.021.489392
ACATGGG137350.020.8203283
CCTGGGG78300.018.7469714
GAGTACA109250.018.7058531
AGTACAT98750.018.5682072
GTATACG2854.141839E-818.3860971
AGAGTAC144150.018.37963310-11
CATGGGG105200.017.4303464
GTGTAGC25200.017.2021431
GTATAAC9650.016.7839181
AGTACTT86950.016.40633812-13
GTACTTT75300.016.29286614-15
GAGTACT76400.016.217512-13
TAACGCA4701.4551915E-1116.1717324
Produced by FastQC (version 0.11.5)