##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921469_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4457262
Sequences flagged as poor quality	0
Sequence length	101
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.7877845188369	33.0	31.0	34.0	30.0	34.0
2	31.897728246623153	34.0	31.0	34.0	30.0	34.0
3	32.027989828733425	34.0	31.0	34.0	30.0	34.0
4	35.57568009239753	37.0	35.0	37.0	33.0	37.0
5	35.357441406854704	37.0	35.0	37.0	33.0	37.0
6	35.39918564356324	37.0	35.0	37.0	33.0	37.0
7	35.324871187738125	37.0	35.0	37.0	33.0	37.0
8	35.274995501722806	37.0	35.0	37.0	33.0	37.0
9	36.86988895873745	39.0	37.0	39.0	33.0	39.0
10-11	36.76898788987499	39.0	37.0	39.0	33.0	39.0
12-13	36.6444967785156	39.0	37.0	39.0	33.0	39.0
14-15	37.88712050133019	40.0	38.0	41.0	33.0	41.0
16-17	37.7927191625711	40.0	38.0	41.0	33.0	41.0
18-19	37.688833862582	40.0	38.0	41.0	32.0	41.0
20-21	37.564257609267756	40.0	38.0	41.0	32.0	41.0
22-23	37.52493200983025	40.0	38.0	41.0	32.0	41.0
24-25	37.57241497134339	40.0	38.0	41.0	32.0	41.0
26-27	37.44487164990525	40.0	38.0	41.0	32.0	41.0
28-29	37.305024923372244	40.0	37.0	41.0	32.0	41.0
30-31	37.164526787969834	40.0	37.0	41.0	31.0	41.0
32-33	36.987510045404555	40.0	37.0	41.0	31.0	41.0
34-35	36.81420735420085	40.0	36.5	41.0	30.0	41.0
36-37	36.69726358917201	40.0	36.0	41.0	30.0	41.0
38-39	36.64772589091689	40.0	36.0	41.0	30.0	41.0
40-41	36.48624166584778	39.5	36.0	41.0	30.0	41.0
42-43	36.22621095641226	39.0	35.5	41.0	30.0	41.0
44-45	35.99175704726355	39.0	35.0	40.0	29.0	41.0
46-47	35.69367147365355	39.0	35.0	40.0	28.0	41.0
48-49	35.321921058264024	38.0	35.0	40.0	27.0	41.0
50-51	35.01572837764529	38.0	34.0	40.0	26.0	41.0
52-53	34.697063690669296	38.0	34.0	40.0	26.0	41.0
54-55	34.35294683148534	38.0	34.0	40.0	24.0	41.0
56-57	33.93828980661222	37.5	33.0	40.0	23.5	41.0
58-59	33.52491484682749	37.0	33.0	39.5	22.5	41.0
60-61	33.04864914828879	36.0	32.0	39.0	21.5	40.5
62-63	32.60383784933441	36.0	31.5	39.0	19.5	40.0
64-65	32.06705439348192	35.0	31.0	38.5	18.0	40.0
66-67	31.5003960951813	35.0	30.5	38.0	14.5	40.0
68-69	30.965234038295257	34.5	30.0	37.0	10.0	39.5
70-71	30.373785296893026	34.0	29.0	36.5	7.5	39.0
72-73	29.79266778573932	34.0	29.0	36.0	7.0	39.0
74-75	29.255295739851057	34.0	28.0	35.5	4.0	38.0
76-77	28.047080360095503	31.5	26.5	34.5	2.0	36.0
78-79	28.969762266611205	33.5	29.0	35.0	2.0	37.0
80-81	28.96828479007965	34.0	29.0	35.0	2.0	36.0
82-83	28.58918804862716	34.0	29.0	35.0	2.0	36.0
84-85	28.05528023706033	33.0	27.5	35.0	2.0	35.5
86-87	27.513872305464652	33.0	26.0	35.0	2.0	35.0
88-89	26.92858541858208	32.5	25.5	35.0	2.0	35.0
90-91	26.38665788549114	32.0	24.5	34.0	2.0	35.0
92-93	25.90048509600737	32.0	24.0	34.0	2.0	35.0
94-95	25.335792354140278	32.0	21.5	34.0	2.0	35.0
96-97	24.48625692185023	31.0	16.5	34.0	2.0	35.0
98-99	23.4050341891502	31.0	2.0	34.0	2.0	35.0
100-101	19.870071133354962	25.5	2.0	32.0	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	35905.0
3	18079.0
4	10724.0
5	8003.0
6	7156.0
7	7092.0
8	8533.0
9	10024.0
10	12038.0
11	14210.0
12	16783.0
13	19778.0
14	22045.0
15	25037.0
16	27979.0
17	33683.0
18	33988.0
19	30239.0
20	30654.0
21	32090.0
22	34415.0
23	37662.0
24	42780.0
25	49360.0
26	57886.0
27	69553.0
28	83704.0
29	100916.0
30	122115.0
31	151976.0
32	193292.0
33	254184.0
34	353120.0
35	515441.0
36	760938.0
37	869240.0
38	346750.0
39	9890.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.06006904902301	17.337021349381754	13.26497450047488	24.337935101120358
2	16.242706845592654	19.22155350078142	38.19169705527744	26.344042598348494
3	21.427577801630523	22.091413550353423	27.492115730486322	28.988892917529736
4	13.969728588130288	14.547307162522067	34.78219974175196	36.70076450759569
5	15.423798059287508	35.68943843809686	32.70298654163831	16.183776960977326
6	35.35922678399967	33.11020218299897	16.188289554593048	15.342281478408317
7	31.691325928631876	30.023034071520804	18.91645338614772	19.369186613699604
8	28.184080440021468	33.227078659003574	18.33381620703828	20.25502469393668
9	26.505557925939232	17.158069659338437	19.091733848330378	37.244638566391956
10-11	27.046123926119975	24.756332677954454	25.69865222512545	22.49889117080012
12-13	27.41829898765145	21.72961834197075	26.353389343110905	24.498693327266892
14-15	23.856413659625584	23.57830096898296	24.490215599073213	28.075069772318244
16-17	24.208312666503577	26.701517861751817	24.899744367213465	24.19042510453114
18-19	24.15325155398346	26.35296718539059	26.12532642349823	23.368454837127718
20-21	24.808455569692704	25.020946709349666	26.01807403381164	24.152523687145987
22-23	24.33518829817833	24.831008987977462	26.142019897244666	24.691782816599538
24-25	24.559272604121816	24.99787633026728	26.146363654624082	24.296487410986817
26-27	24.524657683181868	24.841591831408582	26.62227770935387	24.01147277605568
28-29	24.0939413721424	25.799879312616003	26.140734774774856	23.96544454046674
30-31	24.39379590547351	25.15058487306043	25.966829260412723	24.488789961053335
32-33	24.25684244771582	25.21539932858613	26.119715841048237	24.408042382649814
34-35	24.7251518750657	25.105351389500775	26.18573952283334	23.98375721260019
36-37	24.500348123398357	25.07105509324838	26.375053766722097	24.053543016631167
38-39	24.315577944248147	25.344588165350263	26.33015637441405	24.009677515987544
40-41	24.442590902114112	25.406690973652772	25.98809962991985	24.16261849431326
42-43	24.25585537438689	25.419363639494506	25.76074181294235	24.56403917317625
44-45	24.448230860746396	25.01148864576242	25.860436739073556	24.679843754417632
46-47	24.714142267470493	25.430743183539263	25.880586561384924	23.974527987605324
48-49	24.64707646876564	25.36548767849783	25.812131895725653	24.175303957010872
50-51	24.01533210759938	25.626971442059276	26.122178728074974	24.235517722266366
52-53	24.53568138111589	25.847181029041096	25.440598301672402	24.176539288170613
54-55	24.287298931268115	25.262213325471645	25.771057948987874	24.679429794272366
56-57	24.50272628196391	25.314379209246418	25.694000160419016	24.488894348370653
58-59	25.059591844243762	25.26127696216152	25.459731349006343	24.219399844588374
60-61	24.66491144759354	25.33541949298333	26.05196754269442	23.94770151672871
62-63	24.2193483097408	25.502130003713624	25.971953735668613	24.306567950876964
64-65	24.275575510476987	25.79088833367419	25.666329626338463	24.267206529510364
66-67	24.538216558722585	25.441622831977007	25.383873599881312	24.636287009419096
68-69	24.297411085623512	25.507469602335103	25.638046772960276	24.55707253908111
70-71	24.716498413504052	25.286115641766276	25.59068112856283	24.406704816166844
72-73	24.784125099996892	25.053876849612866	25.856242145706343	24.305755904683902
74-75	24.20611547207251	25.9866597230484	25.48674165475103	24.320483150128062
76-77	24.40201492640595	25.315750678680132	25.54301443008928	24.73921996482464
78-79	24.44814702638041	25.341904453777985	25.77278051455012	24.437168005291486
80-81	24.696498612060115	25.35935675267514	25.587614708291362	24.356529926973387
82-83	24.59666546789431	25.288560962623553	25.795815592531106	24.31895797695103
84-85	24.395816120037466	25.55122564650559	25.750884918617107	24.302073314839838
86-87	24.409266828141988	25.65521436247627	25.614693917339864	24.320824892041877
88-89	24.21919736685122	25.537451981097586	25.39380969167089	24.849540960380303
90-91	24.500382126799604	25.19742094737793	25.492597613066625	24.809599312755847
92-93	24.775866964605374	25.638718264982142	25.602545872048715	23.98286889836377
94-95	24.658847286495583	25.397559949516197	25.574836628803816	24.368756135184405
96-97	24.125998233340415	25.41274969996628	26.087273073204635	24.373978993488667
98-99	24.463952995787107	25.65925221549491	25.40210537085868	24.474689417859302
100-101	24.809658415567785	24.764313214850205	25.75324694410705	24.67278142547496
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	254.0
1	212.5
2	397.0
3	606.5
4	439.5
5	336.0
6	626.5
7	1327.0
8	2028.0
9	2031.0
10	1460.5
11	1044.0
12	974.0
13	1081.5
14	1229.5
15	1331.0
16	1415.5
17	1569.5
18	1765.5
19	1947.5
20	2107.0
21	2315.5
22	2696.0
23	3149.5
24	3710.0
25	4551.0
26	5723.0
27	7405.0
28	9599.5
29	12665.0
30	16543.0
31	20791.5
32	26409.0
33	32716.5
34	39433.5
35	47445.5
36	56633.5
37	66542.0
38	76161.5
39	86578.0
40	98404.0
41	109336.0
42	120655.5
43	133778.0
44	146868.5
45	160396.5
46	171879.0
47	193884.5
48	232081.0
49	236727.0
50	210289.0
51	197693.0
52	194649.0
53	192481.5
54	189888.0
55	183077.5
56	170151.5
57	155884.0
58	144048.0
59	130488.0
60	113380.0
61	96685.5
62	79373.0
63	60778.0
64	45113.0
65	34308.0
66	26160.5
67	20015.0
68	15530.5
69	12038.0
70	9290.0
71	7110.5
72	5375.0
73	4211.0
74	3256.5
75	2429.0
76	1770.0
77	1113.0
78	674.5
79	385.0
80	231.0
81	120.0
82	58.0
83	30.5
84	16.5
85	12.0
86	7.5
87	2.0
88	1.5
89	1.0
90	0.5
91	0.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.31503645062821073
2	0.0
3	0.006416495148815574
4	0.05900483301183552
5	7.403648248633354E-4
6	0.06575785762649806
7	0.07684089470172496
8	0.10661253478032029
9	0.09337570912367278
10-11	0.09797494515691471
12-13	0.09757110979789835
14-15	0.06624021652754539
16-17	0.037063111838613036
18-19	0.039867524054004455
20-21	0.0294238929638868
22-23	0.04310942457499694
24-25	0.0343596584629757
26-27	0.021840762333468395
28-29	0.02514996874763027
30-31	0.0031633769789615237
32-33	0.005597606781921278
34-35	0.008783419058605933
36-37	0.01141956654107387
38-39	0.016747949750317574
40-41	0.02086482688251218
42-43	0.004072006536748345
44-45	0.015435484833514385
46-47	0.025923986519078304
48-49	0.0030287651926227356
50-51	0.007639218874726234
52-53	0.00252397099385228
54-55	0.0045768007355188
56-57	0.004139312429917739
58-59	0.0014919472985882364
60-61	0.006023877438660775
62-63	0.015637402513022567
64-65	0.007347560004325526
66-67	0.04019283587098986
68-69	0.06299831600655291
70-71	0.013124649168031854
72-73	0.03361929363811236
74-75	0.05231911429034237
76-77	0.3034710546519365
78-79	0.07432814135673424
80-81	0.034942976203777114
82-83	0.029491198857056193
84-85	0.020640473905280867
86-87	0.11640554223646714
88-89	0.5234379311783781
90-91	0.13147084465754985
92-93	0.04951470207495095
94-95	0.008682460218851842
96-97	0.005900483301183552
98-99	0.010544589929871746
100-101	0.007639218874726234
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4457262.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.25679742689267
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.44824062882151	37.85913034475101
2	15.2189814965754	15.90590466220337
3	5.218811345614996	8.181551018909158
4	2.37105955800475	4.956159160390076
5	1.3072539798677552	3.4156453205724246
6	0.7675525350260459	2.4065902402412407
7	0.5017198755153661	1.8352791729896605
8	0.3581001079217256	1.4970531838571213
9	0.26140669753532386	1.2294249155222778
>10	1.416430785620791	13.279213183601321
>50	0.09107659295916692	3.2664664974228588
>100	0.03699920627455468	3.243857454445778
>500	0.0012051153028534338	0.42962758270132784
>1k	9.038360798697593E-4	1.2254795434834747
>5k	2.1519906663565198E-4	0.7980337541409915
>10k+	4.3039813327130395E-5	0.47058396476782505
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	20923	0.46941373426107774	No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT	8655	0.19417750179370205	No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC	7434	0.16678400327375864	No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT	7351	0.16492187356273874	No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT	6347	0.14239683464871483	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5695	0.1277690205332332	No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC	4899	0.10991052354562061	No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG	4543	0.10192355755618585	No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT	4479	0.10048769850190542	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	2.2435297723131374E-5	0.0
3	0.0	0.0	0.0	2.2435297723131374E-5	0.0
4	0.0	0.0	0.0	2.2435297723131374E-5	0.0
5	0.0	0.0	0.0	2.2435297723131374E-5	0.0
6	0.0	0.0	0.0	2.2435297723131374E-5	0.0
7	0.0	0.0	0.0	4.487059544626275E-5	0.0
8	0.0	0.0	0.0	4.487059544626275E-5	0.0
9	0.0	0.0	0.0	4.487059544626275E-5	2.2435297723131374E-5
10-11	0.0	0.0	0.0	6.730589316939412E-5	2.2435297723131374E-5
12-13	0.0	3.365294658469706E-5	0.0	1.0095883975409119E-4	1.4582943520035393E-4
14-15	0.0	4.487059544626275E-5	0.0	1.3461178633878825E-4	1.9070003064661667E-4
16-17	0.0	4.487059544626275E-5	0.0	1.7948238178505102E-4	2.467882749544451E-4
18-19	0.0	6.730589316939412E-5	0.0	2.5800592381601084E-4	2.692235726775765E-4
20-21	0.0	8.97411908925255E-5	0.0	2.692235726775765E-4	3.1409416812383924E-4
22-23	0.0	8.97411908925255E-5	0.0	3.253118169854049E-4	3.701824124316677E-4
24-25	0.0	8.97411908925255E-5	0.0	4.2627065673949614E-4	3.814000612932334E-4
26-27	0.0	8.97411908925255E-5	0.0	5.272294964935873E-4	4.0383535901636477E-4
28-29	0.0	8.97411908925255E-5	0.0	0.001009588397540912	4.0383535901636477E-4
30-31	0.0	8.97411908925255E-5	0.0	0.0030512004903458668	4.0383535901636477E-4
32-33	0.0	8.97411908925255E-5	0.0	0.007123207027094212	4.2627065673949614E-4
34-35	0.0	8.97411908925255E-5	0.0	0.012395501992030086	4.935765499088903E-4
36-37	0.0	1.1217648861565688E-4	0.0	0.019328008988477682	4.935765499088903E-4
38-39	0.0	1.3461178633878825E-4	0.0	0.029771640078595334	4.935765499088903E-4
40-41	0.0	1.3461178633878825E-4	0.0	0.04055180063455996	5.160118476320217E-4
42-43	0.0	1.5704708406191962E-4	0.0	0.05124221999963206	5.160118476320217E-4
44-45	0.0	1.5704708406191962E-4	0.0	0.0633909337167077	5.833177408014157E-4
46-47	0.0	1.682647329234853E-4	0.0	0.07626879460978511	6.618412828323756E-4
48-49	0.0	1.79482381785051E-4	0.0	0.08881012603701555	6.954942294170726E-4
50-51	0.0	2.1313532836974804E-4	0.0	0.10182259871643175	7.852354203095981E-4
52-53	0.0	2.467882749544451E-4	0.0	0.11496968318218673	8.076707180327295E-4
54-55	0.0	2.692235726775765E-4	0.0	0.1282513794342805	8.076707180327295E-4
56-57	0.0	2.9165887040070787E-4	0.0	0.14324937596219384	8.525413134789923E-4
58-59	0.0	3.1409416812383924E-4	0.0	0.1577201429936136	9.086295577868206E-4
60-61	0.0	3.1409416812383924E-4	0.0	0.17250500419315715	9.198472066483863E-4
62-63	0.0	3.1409416812383924E-4	0.0	0.18717768890408507	9.871530998177805E-4
64-65	0.0	3.365294658469706E-4	0.0	0.20167089123322796	0.0010320236952640434
66-67	0.0	3.365294658469706E-4	0.0	0.21748777612803555	0.0010320236952640434
68-69	0.0	3.365294658469706E-4	0.0	0.23292326096154994	0.0010320236952640434
70-71	2.2435297723131374E-5	3.365294658469706E-4	0.0	0.24974973425389846	0.001043241344125609
72-73	2.2435297723131374E-5	3.365294658469706E-4	0.0	0.26549931325553666	0.0011442001838797003
74-75	2.2435297723131374E-5	3.365294658469706E-4	0.0	0.2825277042273934	0.0011666354816028315
76-77	2.2435297723131374E-5	3.365294658469706E-4	0.0	0.2989503421607256	0.001177853130464397
78-79	2.2435297723131374E-5	3.365294658469706E-4	0.0	0.3172911980493855	0.0012115060770490943
80-81	2.2435297723131374E-5	3.701824124316677E-4	0.0	0.33697817180143325	0.0012675943213569228
82-83	2.2435297723131374E-5	3.814000612932334E-4	0.0	0.3543879628345832	0.0013685531611110139
84-85	2.2435297723131374E-5	3.814000612932334E-4	0.0	0.3730653481890901	0.0013685531611110139
86-87	2.2435297723131374E-5	3.814000612932334E-4	0.0	0.39222509244464426	0.0014134237565572765
88-89	2.2435297723131374E-5	3.814000612932334E-4	0.0	0.41203546033416927	0.0014582943520035393
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCTG	5640	0.0	36.57215	1
TACCTGG	5640	0.0	35.458694	2
TATCACG	430	0.0	28.72266	2
ACCTGGG	6960	0.0	27.710001	3
GTACATG	13120	0.0	24.32667	1
TACATGG	13645	0.0	22.350191	2
TATAACG	420	0.0	21.48939	2
ACATGGG	13735	0.0	20.820328	3
CCTGGGG	7830	0.0	18.746971	4
GAGTACA	10925	0.0	18.705853	1
AGTACAT	9875	0.0	18.568207	2
GTATACG	285	4.141839E-8	18.386097	1
AGAGTAC	14415	0.0	18.379633	10-11
CATGGGG	10520	0.0	17.430346	4
GTGTAGC	2520	0.0	17.202143	1
GTATAAC	965	0.0	16.783918	1
AGTACTT	8695	0.0	16.406338	12-13
GTACTTT	7530	0.0	16.292866	14-15
GAGTACT	7640	0.0	16.2175	12-13
TAACGCA	470	1.4551915E-11	16.171732	4
>>END_MODULE