FastQCFastQC Report
Mon 15 Aug 2016
SRR2921468_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921468_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4022167
Sequences flagged as poor quality0
Sequence length98
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46920.11665353527091243No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG52850.034.2259451
GTATCAA93600.033.9297871
TACCTGG54000.033.2900052
TCAACGC113400.027.4582524
TATCAAC114450.027.246752
CAACGCA116150.026.729185
ATCAACG117700.026.3385473
ACCTGGG68200.025.4845183
AACGCAG124200.025.137546
GTACATG128800.023.8708841
TACATGG133450.022.30222
ACGCAGA141650.021.9070037
CGCAGAG148650.020.8992088
AGTACAT93550.020.8827362
ACATGGG137400.020.4928913
GAGTACA106100.020.3453581
GGTATCA61350.019.7311
TATACCG3355.638867E-1119.1645835
TATAACG3501.05501385E-1018.3434732
GCAGAGT161900.018.339979
Produced by FastQC (version 0.11.5)