##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921468_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4022167
Sequences flagged as poor quality	0
Sequence length	98
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.622802335159133	31.0	31.0	34.0	28.0	34.0
2	30.723017467947006	31.0	31.0	34.0	27.0	34.0
3	30.858147361857426	31.0	31.0	34.0	27.0	34.0
4	34.37972863881584	37.0	35.0	37.0	31.0	37.0
5	34.37147264148903	37.0	35.0	37.0	31.0	37.0
6	34.47018758793456	37.0	35.0	37.0	32.0	37.0
7	34.36247997658973	37.0	35.0	37.0	31.0	37.0
8	34.34830751681867	37.0	35.0	37.0	31.0	37.0
9	35.828360433567276	39.0	37.0	39.0	31.0	39.0
10-11	35.70815495726558	39.0	37.0	39.0	30.0	39.0
12-13	35.5511474287368	39.0	35.0	39.0	30.0	39.0
14-15	36.6026556082828	40.0	37.0	41.0	30.0	41.0
16-17	36.46801040334725	40.0	36.5	41.0	30.0	41.0
18-19	36.28912461864462	40.0	36.0	41.0	29.5	41.0
20-21	36.17521313759473	39.0	36.0	41.0	29.0	41.0
22-23	36.21338994626528	39.5	36.0	41.0	29.0	41.0
24-25	36.08886913447403	39.0	36.0	41.0	29.0	41.0
26-27	35.82847678875591	39.0	36.0	41.0	27.0	41.0
28-29	35.60048687685021	39.0	35.5	40.0	27.0	41.0
30-31	35.35177231576909	39.0	35.0	40.0	26.5	41.0
32-33	35.0925199028285	38.5	35.0	40.0	25.0	41.0
34-35	34.681193868877145	38.0	34.5	40.0	24.5	41.0
36-37	34.30379394987826	38.0	34.0	40.0	23.0	41.0
38-39	34.071965062614254	38.0	33.5	40.0	21.5	41.0
40-41	33.80449854518721	38.0	33.0	40.0	19.5	41.0
42-43	33.52135242022521	38.0	33.0	40.0	18.0	41.0
44-45	33.198213052814566	38.0	32.5	40.0	15.5	41.0
46-47	32.91629872653224	37.5	32.0	40.0	14.5	41.0
48-49	32.706271892738414	37.0	32.0	40.0	12.0	41.0
50-51	31.943228612834822	36.0	30.5	39.0	11.0	40.0
52-53	32.45229598870459	37.0	31.5	39.0	13.0	40.0
54-55	32.889323963922934	37.0	33.0	40.0	10.5	41.0
56-57	32.730847575448756	37.0	32.0	40.0	8.0	41.0
58-59	32.39571019801018	36.5	32.0	39.5	7.5	41.0
60-61	31.93710790725497	36.0	31.0	39.0	4.0	40.5
62-63	31.44651403086943	35.5	31.0	39.0	2.0	40.0
64-65	30.901751966042184	35.0	30.0	38.5	2.0	40.0
66-67	30.409400703650544	35.0	29.5	38.0	2.0	40.0
68-69	29.882912121749296	34.5	29.0	37.0	2.0	39.5
70-71	29.356978961838234	34.0	28.5	36.5	2.0	39.0
72-73	28.793038802217808	34.0	28.0	36.0	2.0	39.0
74-75	28.194438097672226	33.5	26.5	35.5	2.0	38.0
76-77	27.606154344163233	33.0	26.0	35.0	2.0	37.0
78-79	27.03937678370888	33.0	25.5	35.0	2.0	36.5
80-81	26.452084411214155	32.0	24.5	35.0	2.0	36.0
82-83	25.820573834950164	32.0	22.5	34.5	2.0	35.5
84-85	25.189586981346125	31.5	20.0	34.0	2.0	35.0
86-87	24.558371022386687	31.0	17.5	34.0	2.0	35.0
88-89	23.89284594597887	31.0	9.5	34.0	2.0	35.0
90-91	23.135647401015422	30.0	2.0	34.0	2.0	35.0
92-93	22.29688635504195	29.5	2.0	34.0	2.0	35.0
94-95	21.361496427174707	29.0	2.0	34.0	2.0	35.0
96-97	20.44848423747696	29.0	2.0	33.5	2.0	34.0
98	19.80533379146117	27.0	2.0	33.0	2.0	34.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	105177.0
3	18877.0
4	14698.0
5	12657.0
6	11908.0
7	12345.0
8	14337.0
9	16992.0
10	18793.0
11	20574.0
12	23220.0
13	27334.0
14	23340.0
15	22587.0
16	25124.0
17	28398.0
18	32393.0
19	32251.0
20	34166.0
21	37233.0
22	40946.0
23	45378.0
24	50713.0
25	57049.0
26	65218.0
27	74590.0
28	86367.0
29	101625.0
30	121640.0
31	147185.0
32	184030.0
33	238376.0
34	321743.0
35	450583.0
36	606856.0
37	627630.0
38	259706.0
39	10128.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.4157181301082	16.682646619921453	13.025081325432852	24.87655392453749
2	16.270264704082713	18.55950655827982	38.63293680700545	26.53729193063202
3	20.66489855232529	22.060037282636113	26.958320958025446	30.316743207013154
4	14.103007379275246	14.317102377058927	33.90989155206561	37.66999869160022
5	16.05803226259054	35.18112008565677	32.12442322484604	16.636424426906654
6	35.854175814470715	32.293031581225655	16.511698201453616	15.341094402850011
7	32.04561593324255	29.62332033751811	18.85206533972553	19.47899838951381
8	28.413140529181103	33.113842097109455	18.592905343658373	19.88011203005107
9	27.167021366797727	16.659368770524555	18.64059216646066	37.53301769621705
10-11	27.03246420063385	24.636349406438633	25.630353182782684	22.70083321014483
12-13	27.14493725509795	21.67562915847063	26.3416697312446	24.83776385518682
14-15	24.184735681332366	23.302523414531226	23.9007065481117	28.612034356024708
16-17	24.71547606318831	26.358553621292856	24.44552333257908	24.480446982939753
18-19	24.720508544164346	25.63529152620836	25.881347240545427	23.76285268908187
20-21	25.19956316437595	24.860117300666378	25.792983806775133	24.147335728182544
22-23	24.970690334392735	24.509885899481347	25.666183626454874	24.85324013967104
24-25	24.66489546440197	24.81136919789864	25.803484619449424	24.720250718249968
26-27	24.747859420849892	24.962147498972975	25.7469788292237	24.543014250953433
28-29	24.692584645362423	25.106021704356984	25.677637535222892	24.5237561150577
30-31	24.87795965255566	25.036505138580225	25.5361342413971	24.54940096746702
32-33	24.694455546058045	25.059793277039656	25.500424403565635	24.74532677333666
34-35	24.818153650759424	25.03404452161161	25.66923861438017	24.478563213248794
36-37	24.859146149227733	24.95691503523644	25.600541443040242	24.58339737249558
38-39	24.824731660196914	25.022899129833064	25.57221575580128	24.58015345416874
40-41	24.961628606910953	25.000974908358614	25.531787511973874	24.50560897275656
42-43	24.912405482891952	24.89482410121278	25.489292226305242	24.703478189590022
44-45	24.814028718913374	25.01650440458955	25.486298970355044	24.683167906142035
46-47	24.970355331268244	25.00236107981049	25.435263189191705	24.592020399729563
48-49	24.918127967576215	25.115442782263802	25.28781218767492	24.678617062485063
50-51	24.78449288239333	25.274549821247778	25.286255918012845	24.654701378346044
52-53	24.87313274962979	25.20396032869708	25.2863671530974	24.63653976857573
54-55	24.84693211765653	25.017974164201362	25.36853286322186	24.766560854920247
56-57	24.801290789940406	25.16726782004655	25.353667223028715	24.677774166984328
58-59	24.929879230136777	25.084860684076922	25.344183242616243	24.64107684317006
60-61	24.945199808008812	25.036672965596747	25.424678063707397	24.593449162687048
62-63	24.752946203670383	25.213006277238016	25.382331774019427	24.651715745072167
64-65	24.829005676066792	25.048700203198692	25.400823791992522	24.721470328741994
66-67	24.828552057375312	25.178710355475737	25.294058971292344	24.698678615856608
68-69	24.720289641580166	25.17605006880074	25.373724924711503	24.729935364907586
70-71	24.743555752981802	24.993084600407293	25.41792429114188	24.84543535546902
72-73	24.839077577892795	25.067525485626614	25.29179050917313	24.801606427307465
74-75	24.647020218835667	25.29287593740212	25.311926402784014	24.7481774409782
76-77	24.846277472657814	25.065374525110762	25.226875572151954	24.861472430079473
78-79	24.826341160122702	24.95925549991448	25.409348949457346	24.805054390505475
80-81	24.740472441220135	25.14527438303098	25.304778133603868	24.80947504214502
82-83	24.919596601954357	25.05115898769887	25.242958365549633	24.786286044797144
84-85	24.871820221510035	25.11695772844736	25.191692337574246	24.81952971246836
86-87	24.782332707298394	25.097971659279704	25.39516036917961	24.724535264242295
88-89	24.81063599982519	24.9339027176865	25.30726152347774	24.94819975901057
90-91	24.8360675794581	24.979862691772592	25.27757775023446	24.906491978534852
92-93	24.878185068312614	25.07780288570713	25.318842336504705	24.725169709475555
94-95	24.962965894663455	24.93141589578088	25.253433138036304	24.852185071519365
96-97	24.895277320188043	24.94440362723183	25.279161644276847	24.88115740830328
98	24.899418005138333	25.000087386615867	25.18000394488152	24.920490663364284
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	535.0
1	413.0
2	515.5
3	756.5
4	609.0
5	507.0
6	893.0
7	1826.5
8	2628.5
9	2559.5
10	1980.5
11	1636.5
12	1765.0
13	2032.0
14	2325.0
15	2620.5
16	2879.5
17	3087.5
18	3084.0
19	2955.5
20	2823.0
21	2651.5
22	2467.0
23	2496.5
24	2647.5
25	2861.5
26	3381.5
27	4209.5
28	5481.5
29	7347.0
30	9960.5
31	13814.0
32	19392.0
33	26001.5
34	33645.5
35	43226.5
36	53272.0
37	64040.5
38	75718.5
39	86429.5
40	97111.0
41	108965.0
42	121374.0
43	133722.5
44	145495.0
45	157062.5
46	169984.5
47	182781.5
48	188592.0
49	187141.0
50	185704.0
51	185096.0
52	185258.5
53	185992.5
54	183537.5
55	176898.0
56	166431.5
57	154785.5
58	142207.0
59	127915.0
60	111801.5
61	94036.5
62	76315.0
63	58706.0
64	43886.5
65	33470.0
66	25555.5
67	19512.5
68	15175.5
69	11748.0
70	8802.5
71	6649.5
72	5250.0
73	3973.5
74	3091.5
75	2345.5
76	1599.5
77	1002.0
78	599.5
79	374.0
80	235.0
81	156.5
82	99.0
83	55.0
84	32.5
85	26.5
86	21.5
87	18.0
88	12.0
89	9.0
90	11.0
91	8.0
92	5.0
93	4.0
94	4.0
95	4.5
96	5.0
97	4.0
98	2.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.45229350248261696
2	0.04358347129793467
3	0.05134048387349406
4	0.04945095516919113
5	0.0597439141636834
6	0.06295114051703969
7	0.040823764900860655
8	0.018547215965920856
9	0.011436621104991414
10-11	0.013736376435886426
12-13	0.010094061236144595
14-15	0.014233620831755616
16-17	0.013351012029087802
18-19	0.053267305907487184
20-21	0.08115028540585212
22-23	0.09616706616110171
24-25	0.06650643794750442
26-27	0.08149835648296055
28-29	0.08195830754913956
30-31	0.054323950248709216
32-33	0.030580530345955304
34-35	0.3799320117737528
36-37	0.5957609418007755
38-39	0.547341768753013
40-41	0.541772631519278
42-43	0.5165126162091231
44-45	0.5023411509268512
46-47	0.491302325338555
48-49	0.48298591281764286
50-51	0.49672228925352924
52-53	0.574441588327884
54-55	0.5464964532800354
56-57	0.5041187996420835
58-59	0.48307293058691997
60-61	0.49475817388984594
62-63	0.50736331932513
64-65	0.5260099841702247
66-67	0.5499274396115328
68-69	0.5070276793579183
70-71	0.45799938192521594
72-73	0.44137898799328823
74-75	0.42304310089561176
76-77	0.43630709515542243
78-79	0.4309119934602417
80-81	0.4287614114481074
82-83	0.4470972985457839
84-85	0.41420458175903685
86-87	0.4176728614202245
88-89	0.4435917255549061
90-91	0.426710278315147
92-93	0.42299337645602486
94-95	0.43291340215361523
96-97	0.4272448160407064
98	0.42208590543356356
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	4022167.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.5197251469677
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.71539085374755	41.48177964448329
2	13.817614844264966	15.343003566805042
3	4.821191865274416	8.030137417224966
4	2.1215050449271127	4.7114150796903465
5	1.194029898291784	3.314610588521083
6	0.7411444114256077	2.4688880409936536
7	0.503406993660744	1.9564312547574076
8	0.34216036609628003	1.519731958548105
9	0.2586227085453617	1.2922795525682536
>10	1.3749392073954536	13.653001949894467
>50	0.08006838869209022	3.044855856000099
>100	0.028934906134347217	2.5803677340969853
>500	7.65395314610145E-4	0.2785891786904041
>1k	2.2511622977969612E-4	0.3249081777259353
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4692	0.11665353527091243	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	2.4862219793459597E-5
9	0.0	0.0	0.0	0.0	4.972443958691919E-5
10-11	0.0	0.0	0.0	0.0	6.215554948364899E-5
12-13	0.0	0.0	0.0	0.0	1.9889775834767677E-4
14-15	0.0	0.0	0.0	0.0	2.2375997814113636E-4
16-17	0.0	0.0	0.0	1.2431109896729798E-5	2.4862219793459595E-4
18-19	0.0	2.4862219793459597E-5	0.0	2.4862219793459597E-5	2.7348441772805554E-4
20-21	0.0	2.4862219793459597E-5	0.0	2.4862219793459597E-5	3.232088573149747E-4
22-23	0.0	2.4862219793459597E-5	0.0	4.972443958691919E-5	3.356399672117045E-4
24-25	2.4862219793459597E-5	2.4862219793459597E-5	0.0	9.944887917383839E-5	3.480710771084343E-4
26-27	2.4862219793459597E-5	4.972443958691919E-5	0.0	1.6160442865748736E-4	4.475199562822727E-4
28-29	2.4862219793459597E-5	4.972443958691919E-5	0.0	4.599510661790025E-4	4.8481328597246214E-4
30-31	2.4862219793459597E-5	4.972443958691919E-5	0.0	0.001964115363683308	4.972443958691919E-4
32-33	2.4862219793459597E-5	4.972443958691919E-5	0.0	0.005867483871256464	5.469688354561111E-4
34-35	2.4862219793459597E-5	4.972443958691919E-5	0.0	0.010728047840877816	5.718310552495707E-4
36-37	2.4862219793459597E-5	4.972443958691919E-5	0.0	0.018348318207573183	5.718310552495707E-4
38-39	2.4862219793459597E-5	4.972443958691919E-5	0.0	0.029436868235456163	5.718310552495707E-4
40-41	2.4862219793459597E-5	4.972443958691919E-5	0.0	0.042327929198364964	5.718310552495707E-4
42-43	2.4862219793459597E-5	4.972443958691919E-5	0.0	0.0547341768753013	5.966932750430304E-4
44-45	2.4862219793459597E-5	4.972443958691919E-5	0.0	0.06735175342048204	6.464177146299494E-4
46-47	2.4862219793459597E-5	4.972443958691919E-5	0.0	0.08123730317512923	7.210043740103283E-4
48-49	2.4862219793459597E-5	7.45866593803788E-5	0.0	0.09477478185266797	7.707288135972474E-4
50-51	2.4862219793459597E-5	9.944887917383839E-5	0.0	0.10781501613433753	7.955910333907071E-4
52-53	2.4862219793459597E-5	9.944887917383839E-5	0.0	0.1216135481197076	8.204532531841666E-4
54-55	2.4862219793459597E-5	9.944887917383839E-5	0.0	0.13640656889681607	8.204532531841666E-4
56-57	2.4862219793459597E-5	9.944887917383839E-5	0.0	0.1514855052015493	8.701776927710858E-4
58-59	2.4862219793459597E-5	1.1187998907056818E-4	0.0	0.16768324139698826	8.701776927710858E-4
60-61	2.4862219793459597E-5	1.2431109896729798E-4	0.0	0.18359506206480236	8.701776927710858E-4
62-63	2.4862219793459597E-5	1.2431109896729798E-4	0.0	0.20094889148063716	9.696265719449242E-4
64-65	2.4862219793459597E-5	1.2431109896729798E-4	0.0	0.21771845873132567	9.82057681841654E-4
66-67	2.4862219793459597E-5	1.2431109896729798E-4	0.0	0.2350847192570572	0.0010193510115318433
68-69	2.4862219793459597E-5	1.2431109896729798E-4	0.0	0.2528860686291743	0.0010193510115318433
70-71	2.4862219793459597E-5	1.2431109896729798E-4	0.0	0.27160732013364935	0.001044213231325303
72-73	2.4862219793459597E-5	1.2431109896729798E-4	0.0	0.2899929316709127	0.0011187998907056817
74-75	2.4862219793459597E-5	1.2431109896729798E-4	0.0	0.3092735831207406	0.0011436621104991415
76-77	2.4862219793459597E-5	1.2431109896729798E-4	0.0	0.32946170559302984	0.0011436621104991415
78-79	2.4862219793459597E-5	1.2431109896729798E-4	0.0	0.3509053701648887	0.0011436621104991415
80-81	2.4862219793459597E-5	1.2431109896729798E-4	0.0	0.3721128436487098	0.001168524330292601
82-83	3.72933296901894E-5	1.2431109896729798E-4	0.0	0.3937926993086065	0.0013176976490533586
84-85	4.972443958691919E-5	1.2431109896729798E-4	0.0	0.4154352616388131	0.0013176976490533586
86	4.972443958691919E-5	1.2431109896729798E-4	0.0	0.432876108823925	0.0013176976490533586
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCTG	5285	0.0	34.225945	1
GTATCAA	9360	0.0	33.929787	1
TACCTGG	5400	0.0	33.290005	2
TCAACGC	11340	0.0	27.458252	4
TATCAAC	11445	0.0	27.24675	2
CAACGCA	11615	0.0	26.72918	5
ATCAACG	11770	0.0	26.338547	3
ACCTGGG	6820	0.0	25.484518	3
AACGCAG	12420	0.0	25.13754	6
GTACATG	12880	0.0	23.870884	1
TACATGG	13345	0.0	22.3022	2
ACGCAGA	14165	0.0	21.907003	7
CGCAGAG	14865	0.0	20.899208	8
AGTACAT	9355	0.0	20.882736	2
ACATGGG	13740	0.0	20.492891	3
GAGTACA	10610	0.0	20.345358	1
GGTATCA	6135	0.0	19.731	1
TATACCG	335	5.638867E-11	19.164583	5
TATAACG	350	1.05501385E-10	18.343473	2
GCAGAGT	16190	0.0	18.33997	9
>>END_MODULE