FastQCFastQC Report
Mon 15 Aug 2016
SRR2921468_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921468_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4022167
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49790.12378899235163533No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA90800.034.560021
GTACCTG52350.033.200961
TACCTGG55800.030.7274592
TCAACGC109450.028.2948324
TATCAAC115500.027.181482
CAACGCA114350.027.1532765
ATCAACG113900.027.1052933
GTACATG128900.026.1180631
AACGCAG121250.025.6879676
TACATGG132500.024.5184972
ACCTGGG70000.023.4769943
CGTATAC2303.6361598E-922.7158663
ACATGGG139650.022.5154953
GTATAGG10400.022.4355741
GGTATCA59300.022.3236141
ACGCAGA142700.021.5610317
CGCAGAG150350.020.4323988
CATGGGG107300.019.7871424
GAGTACA104250.019.1386991
AGTACAT94450.018.4048712
Produced by FastQC (version 0.11.5)