##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921468_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4022167
Sequences flagged as poor quality	0
Sequence length	101
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.999928148184797	33.0	31.0	34.0	30.0	34.0
2	32.11227455249869	34.0	31.0	34.0	30.0	34.0
3	32.239100216375896	34.0	31.0	34.0	30.0	34.0
4	35.78127089203407	37.0	35.0	37.0	35.0	37.0
5	35.59192569577544	37.0	35.0	37.0	33.0	37.0
6	35.6297756905668	37.0	35.0	37.0	35.0	37.0
7	35.565158035457	37.0	35.0	37.0	33.0	37.0
8	35.51663841904128	37.0	35.0	37.0	33.0	37.0
9	37.14814775219428	39.0	37.0	39.0	34.0	39.0
10-11	37.06000459453822	39.0	37.0	39.0	34.0	39.0
12-13	36.94462301043194	39.0	37.0	39.0	33.0	39.0
14-15	38.256386171931695	40.0	38.0	41.0	33.5	41.0
16-17	38.163048799316385	40.0	38.0	41.0	33.0	41.0
18-19	38.056268175836564	40.0	38.0	41.0	33.0	41.0
20-21	37.94071268050283	40.0	38.0	41.0	33.0	41.0
22-23	37.90785464651269	40.0	38.0	41.0	33.0	41.0
24-25	37.96644271607818	40.0	38.0	41.0	33.0	41.0
26-27	37.862059432141926	40.0	38.0	41.0	33.0	41.0
28-29	37.72554707449989	40.0	38.0	41.0	33.0	41.0
30-31	37.585395633746685	40.0	38.0	41.0	32.5	41.0
32-33	37.42495189782026	40.0	37.0	41.0	32.0	41.0
34-35	37.28225904095976	40.0	37.0	41.0	32.0	41.0
36-37	37.188268910763774	40.0	37.0	41.0	31.5	41.0
38-39	37.157988591721825	40.0	37.0	41.0	31.0	41.0
40-41	36.993708242347964	40.0	37.0	41.0	31.0	41.0
42-43	36.75645317561404	39.5	36.0	41.0	30.5	41.0
44-45	36.55203463705013	39.0	36.0	41.0	30.0	41.0
46-47	36.28030946999466	39.0	35.5	40.5	30.0	41.0
48-49	35.951646711834684	39.0	35.0	40.0	29.0	41.0
50-51	35.673195568458496	38.5	35.0	40.0	28.5	41.0
52-53	35.36300108871661	38.0	35.0	40.0	28.0	41.0
54-55	35.03306936285838	38.0	34.0	40.0	27.0	41.0
56-57	34.64940739656011	38.0	34.0	40.0	26.0	41.0
58-59	34.25953832846821	37.0	33.5	40.0	26.0	41.0
60-61	33.82610294401003	36.5	33.0	39.0	25.5	41.0
62-63	33.37794228832368	36.0	32.5	39.0	24.0	40.0
64-65	32.85793193072291	35.5	32.0	39.0	22.5	40.0
66-67	32.32612768191873	35.0	31.0	38.0	21.5	40.0
68-69	31.81785415672696	35.0	31.0	37.5	20.0	39.5
70-71	31.26584027963036	34.5	30.5	37.0	18.5	39.0
72-73	30.711936625207258	34.0	30.0	36.0	17.5	39.0
74-75	30.187932773552166	34.0	29.0	35.5	13.5	38.0
76-77	28.9162191674289	32.5	28.0	34.5	12.0	36.0
78-79	29.86448934119344	34.0	29.5	35.0	12.0	37.0
80-81	29.90112270325921	34.0	30.0	35.0	10.5	36.5
82-83	29.561240122550856	34.0	29.5	35.0	7.0	36.0
84-85	29.063125673299993	34.0	29.0	35.0	3.5	35.5
86-87	28.5352215609148	33.0	29.0	35.0	2.0	35.0
88-89	27.986650976948496	33.0	27.0	35.0	2.0	35.0
90-91	27.503945385659023	33.0	26.5	35.0	2.0	35.0
92-93	27.062074001402728	32.5	25.5	34.0	2.0	35.0
94-95	26.553554464546103	32.0	25.0	34.0	2.0	35.0
96-97	25.75788175876337	32.0	23.5	34.0	2.0	35.0
98-99	24.717272927752624	32.0	17.5	34.0	2.0	35.0
100-101	21.099440301708007	27.0	2.0	32.5	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	23187.0
3	12401.0
4	7313.0
5	5449.0
6	4926.0
7	5122.0
8	5935.0
9	7139.0
10	8596.0
11	10113.0
12	11950.0
13	14002.0
14	15963.0
15	17428.0
16	20189.0
17	23596.0
18	24281.0
19	22183.0
20	22456.0
21	23370.0
22	25510.0
23	28266.0
24	32636.0
25	37880.0
26	45558.0
27	55258.0
28	67591.0
29	82880.0
30	102262.0
31	127722.0
32	164397.0
33	221383.0
34	314940.0
35	473506.0
36	727823.0
37	867856.0
38	351810.0
39	9290.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.711516160551604	17.185783123756494	13.401479266182623	24.70122144950928
2	16.41930829823824	18.83146572481948	38.303755164815385	26.4454708121269
3	20.699183996089427	22.24001639019403	27.177935298018337	29.88286431569821
4	14.291734204181704	14.645491479079043	33.9313201929956	37.13145412374365
5	16.074528373659234	35.15503912587957	32.24611936016246	16.524313140298734
6	35.778040550542386	32.5002917009887	16.403820648548948	15.317847099919964
7	32.114921354330725	29.65065389187707	18.819725286290737	19.414699467501467
8	28.4491072524091	33.19411775393114	18.58633759325872	19.770437400401047
9	27.083614848260655	16.64537729470438	18.692491632940865	37.578516224094095
10-11	27.007431421762895	24.739431131588663	25.64389873218001	22.609238714468432
12-13	27.141168433797002	21.70992102352123	26.41804899353829	24.73086154914348
14-15	24.080965403040743	23.3364779686452	24.028123588148308	28.55443304016575
16-17	24.67794891105135	26.4126412404964	24.558902861258687	24.350506987193565
18-19	24.678632484797326	25.677341256877252	25.989248099440093	23.65477815888533
20-21	25.194723965667855	24.819171788200112	25.97953873557966	24.00656551055238
22-23	24.92505840121936	24.460970176576634	25.92553993767884	24.688431484525168
24-25	24.616318035962195	24.790649968737394	25.95944118277607	24.633590812524343
26-27	24.649928207617503	24.94618679746292	26.00830125844313	24.395583736476446
28-29	24.604642429939545	25.127525073548245	25.85614385862821	24.411688637883998
30-31	24.828227843411916	24.965023994024925	25.76046551466506	24.446282647898094
32-33	24.576738852530816	24.98707407379321	25.75682401259785	24.679363061078124
34-35	24.722551950179945	24.980469109366524	25.897600447954837	24.3993784924987
36-37	24.767016845900418	25.018499409461054	25.791309753216886	24.423173991421645
38-39	24.757574996524976	25.037976622464008	25.804110998910122	24.400337382100894
40-41	24.892132934299998	24.93138557984376	25.741317978756072	24.435163507100167
42-43	24.80823444160771	24.96115460003006	25.652558587590086	24.57805237077215
44-45	24.70406086725595	24.98421028027623	25.68097206985074	24.630756782617087
46-47	24.803096590008327	25.016702114233386	25.685358652490958	24.494842643267333
48-49	24.804894609897374	25.088891082349157	25.503467504314337	24.602746803439135
50-51	24.69297540048768	25.30773321823045	25.452316300542854	24.546975080739024
52-53	24.802039936237076	25.24367638537858	25.389807006885608	24.564476671498735
54-55	24.818858956878213	25.040054659319082	25.45555648378271	24.68552990001999
56-57	24.770548109965848	25.250566846770557	25.41993403580604	24.55895100745755
58-59	24.87303328073251	25.133033176309887	25.405688099078173	24.58824544387943
60-61	24.908399817253393	25.07018650720886	25.495302355504307	24.526111320033444
62-63	24.713612796070827	25.215886823422313	25.483988437962463	24.586511942544398
64-65	24.78809871901977	25.0880798225723	25.458400465449337	24.665420992958587
66-67	24.795464191730225	25.20339792646846	25.353138197174896	24.64799968462642
68-69	24.691008206652864	25.230174241954025	25.417699438009027	24.66111811338408
70-71	24.731055395209854	25.03274429857579	25.4998486333801	24.73635167283426
72-73	24.915538366515925	25.02711762776994	25.365473996734817	24.691870008979315
74-75	24.620852995099344	25.30643251768486	25.305362912639012	24.767351574576782
76-77	24.788221200208902	25.13054814825158	25.31394203730788	24.76728861423164
78-79	24.704574835568057	25.058244088759913	25.51444383737235	24.72273723829968
80-81	24.672117030490575	25.23566947889523	25.37285499955544	24.719358491058756
82-83	24.805032316178032	25.145751695265737	25.327408182587092	24.72180780596914
84-85	24.762008729009427	25.203556904112318	25.278530448895086	24.75590391798317
86-87	24.739775332359258	25.19268249578717	25.376478846244794	24.691063325608773
88-89	24.743818619008596	25.05903899585346	25.359538674822286	24.837603710315662
90-91	24.729436293196	25.142064072443247	25.325537178509748	24.802962455851006
92-93	24.821990807646596	25.251262468603073	25.370780971957803	24.55596575179253
94-95	24.840231729726725	25.076479288524745	25.302619534514108	24.78066944723442
96-97	24.71191220380358	25.114689363445752	25.413884952849997	24.759513479900676
98-99	24.71401545267009	25.161542899888843	25.315629495461504	24.80881215197956
100-101	25.254617096887085	24.59421575398027	25.41201890412986	24.73914824500279
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	277.0
1	234.0
2	398.5
3	582.0
4	431.0
5	354.5
6	694.0
7	1612.5
8	2414.0
9	2293.5
10	1624.5
11	1134.5
12	1081.0
13	1182.0
14	1221.0
15	1225.0
16	1264.0
17	1401.0
18	1539.0
19	1575.0
20	1601.0
21	1667.0
22	1778.0
23	1925.5
24	2121.5
25	2467.0
26	2918.5
27	3578.0
28	4714.5
29	6305.0
30	8692.5
31	12085.5
32	16745.0
33	22636.5
34	29185.5
35	36782.5
36	46611.5
37	57011.5
38	67275.0
39	77577.5
40	88067.5
41	98878.0
42	109629.0
43	121822.0
44	134711.5
45	147086.0
46	157932.0
47	169077.0
48	182211.0
49	187802.5
50	184872.5
51	183292.5
52	183263.5
53	182559.0
54	180910.0
55	174947.0
56	163974.0
57	151630.5
58	139705.0
59	126874.5
60	111143.5
61	94060.5
62	77188.0
63	59627.0
64	44379.5
65	33791.5
66	25792.0
67	19825.0
68	15334.0
69	11623.5
70	8783.0
71	6826.0
72	5188.0
73	3977.5
74	3053.0
75	2259.0
76	1641.0
77	1025.5
78	587.5
79	319.0
80	192.0
81	114.0
82	57.0
83	21.5
84	11.0
85	8.5
86	5.0
87	4.0
88	3.0
89	1.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.5
95	0.5
96	0.5
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.30682465447108487
2	0.0
3	0.006215554948364899
4	0.05417477692994845
5	5.221066156626515E-4
6	0.06563626025473333
7	0.07461152160017225
8	0.10387435429707419
9	0.09273607982960429
10-11	0.09675132832624801
12-13	0.09651513723821015
14-15	0.06521360251824453
16-17	0.03614966757969025
18-19	0.03917042728459559
20-21	0.028703432751549105
22-23	0.042340360308261694
24-25	0.03453362329311538
26-27	0.021108024604647196
28-29	0.024787633134079216
30-31	0.0030829152543889898
32-33	0.0052583594863167046
34-35	0.008279119191222045
36-37	0.010367545653872651
38-39	0.01526540295318419
40-41	0.019442255878485405
42-43	0.0033191063424268556
44-45	0.013997429743717753
46-47	0.024103922089759075
48-49	0.0027348441772805554
50-51	0.006414452706712576
52-53	0.0020014086933734976
54-55	0.004052541826333914
56-57	0.0033936930018072346
58-59	0.0012431109896729798
60-61	0.005084323947762487
62-63	0.013860687534853724
64-65	0.00614096828898452
66-67	0.03825052515223759
68-69	0.06067624740593815
70-71	0.01179712329199658
72-73	0.0312891036100689
74-75	0.049513110718674785
76-77	0.2902291227589506
78-79	0.07147888190619633
80-81	0.033178632314371834
82-83	0.02774623728950091
84-85	0.018609371515404507
86-87	0.11628060197401054
88-89	0.5220568912230646
90-91	0.13001697840989695
92-93	0.04646748879397598
94-95	0.008117514762564558
96-97	0.005370239475387273
98-99	0.010007043466867488
100-101	0.007321923729173851
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4022167.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.86503745379074
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.34732597936208	37.00431734147432
2	15.489508654054909	16.067278929667474
3	5.485796998248054	8.535632003340845
4	2.4910380205281677	5.1679112093404065
5	1.3453273491252873	3.488772667499602
6	0.8267353861506066	2.5727197060245217
7	0.57903020230934	2.1021996190754964
8	0.40505999705920437	1.6806761534806405
9	0.3018516449570167	1.4089992184065616
>10	1.5950428353231911	14.87437890199293
>50	0.0954784019617442	3.376781445728045
>100	0.036507740835497786	3.0189806779152004
>500	0.0010086145469939455	0.3284933819966272
>1k	2.88175537848248E-4	0.37285874405740577
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4979	0.12378899235163533	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.4862219793459597E-5
2	0.0	0.0	0.0	2.4862219793459597E-5	2.4862219793459597E-5
3	0.0	0.0	0.0	2.4862219793459597E-5	2.4862219793459597E-5
4	0.0	0.0	0.0	2.4862219793459597E-5	2.4862219793459597E-5
5	0.0	0.0	0.0	2.4862219793459597E-5	2.4862219793459597E-5
6	0.0	0.0	0.0	2.4862219793459597E-5	4.972443958691919E-5
7	0.0	0.0	0.0	4.972443958691919E-5	4.972443958691919E-5
8	0.0	0.0	0.0	4.972443958691919E-5	4.972443958691919E-5
9	0.0	0.0	0.0	4.972443958691919E-5	4.972443958691919E-5
10-11	0.0	0.0	0.0	4.972443958691919E-5	4.972443958691919E-5
12-13	0.0	0.0	0.0	4.972443958691919E-5	1.2431109896729798E-4
14-15	0.0	0.0	0.0	4.972443958691919E-5	1.4917331876075757E-4
16-17	0.0	0.0	0.0	8.701776927710859E-5	1.7403553855421716E-4
18-19	0.0	0.0	0.0	1.2431109896729798E-4	1.9889775834767677E-4
20-21	0.0	0.0	0.0	1.2431109896729798E-4	2.7348441772805554E-4
22-23	0.0	0.0	0.0	1.4917331876075757E-4	3.3563996721170454E-4
24-25	0.0	0.0	0.0	1.9889775834767677E-4	3.9779551669535354E-4
26-27	0.0	0.0	0.0	2.4862219793459595E-4	4.3508884638554296E-4
28-29	0.0	2.4862219793459597E-5	0.0	5.469688354561111E-4	4.475199562822727E-4
30-31	2.4862219793459597E-5	2.4862219793459597E-5	0.0	0.002075995352753876	4.475199562822727E-4
32-33	2.4862219793459597E-5	3.72933296901894E-5	0.0	0.0061036749592943305	4.723821760757323E-4
34-35	2.4862219793459597E-5	4.972443958691919E-5	0.0	0.010989101148709142	4.972443958691919E-4
36-37	2.4862219793459597E-5	4.972443958691919E-5	0.0	0.01841047375705683	5.221066156626515E-4
38-39	2.4862219793459597E-5	4.972443958691919E-5	0.0	0.029412006015662698	5.469688354561111E-4
40-41	2.4862219793459597E-5	4.972443958691919E-5	0.0	0.042129031440017284	5.469688354561111E-4
42-43	2.4862219793459597E-5	4.972443958691919E-5	0.0	0.05424936358932883	5.593999453528409E-4
44-45	2.4862219793459597E-5	4.972443958691919E-5	0.0	0.06676749125533574	5.966932750430304E-4
46-47	2.4862219793459597E-5	4.972443958691919E-5	0.0	0.0805162988011189	6.837110443201389E-4
48-49	2.4862219793459597E-5	4.972443958691919E-5	0.0	0.09390460415989689	7.210043740103283E-4
50-51	2.4862219793459597E-5	4.972443958691919E-5	0.0	0.10680809623270243	7.210043740103283E-4
52-53	2.4862219793459597E-5	4.972443958691919E-5	0.0	0.12044502378941502	7.210043740103283E-4
54-55	2.4862219793459597E-5	4.972443958691919E-5	0.0	0.13486511126962158	7.210043740103283E-4
56-57	2.4862219793459597E-5	4.972443958691919E-5	0.0	0.14979487425559407	7.707288135972476E-4
58-59	2.4862219793459597E-5	4.972443958691919E-5	0.0	0.1657937126926853	8.080221432874369E-4
60-61	2.4862219793459597E-5	4.972443958691919E-5	0.0	0.18133260006359758	8.204532531841666E-4
62-63	2.4862219793459597E-5	4.972443958691919E-5	0.0	0.19820161619345988	8.950399125645454E-4
64-65	2.4862219793459597E-5	6.215554948364899E-5	0.0	0.21458581903734977	9.074710224612752E-4
66-67	2.4862219793459597E-5	7.458665938037878E-5	0.0	0.23160400848597285	9.19902132358005E-4
68-69	2.4862219793459597E-5	7.458665938037878E-5	0.0	0.24894540679191093	9.19902132358005E-4
70-71	2.4862219793459597E-5	7.458665938037878E-5	0.0	0.26710725835103316	9.19902132358005E-4
72-73	2.4862219793459597E-5	7.458665938037878E-5	0.0	0.2850080566023241	0.0010193510115318433
74-75	2.4862219793459597E-5	7.458665938037878E-5	0.0	0.30376660143648937	0.0010939376709122222
76-77	2.4862219793459597E-5	7.458665938037878E-5	0.0	0.3233455995238388	0.0011312310006024117
78-79	2.4862219793459597E-5	7.458665938037878E-5	0.0	0.3441552774909644	0.0011436621104991415
80-81	2.4862219793459597E-5	7.458665938037878E-5	0.0	0.36487793768881305	0.0011809554401893308
82-83	2.4862219793459597E-5	7.458665938037878E-5	0.0	0.38602325562315043	0.0013176976490533586
84-85	2.4862219793459597E-5	7.458665938037878E-5	0.0	0.40687022691996627	0.0013176976490533586
86-87	2.4862219793459597E-5	7.458665938037878E-5	0.0	0.4289851714262486	0.0013798531985370075
88-89	2.4862219793459597E-5	7.458665938037878E-5	0.0	0.4510628226028407	0.0014420087480206565
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	9080	0.0	34.56002	1
GTACCTG	5235	0.0	33.20096	1
TACCTGG	5580	0.0	30.727459	2
TCAACGC	10945	0.0	28.294832	4
TATCAAC	11550	0.0	27.18148	2
CAACGCA	11435	0.0	27.153276	5
ATCAACG	11390	0.0	27.105293	3
GTACATG	12890	0.0	26.118063	1
AACGCAG	12125	0.0	25.687967	6
TACATGG	13250	0.0	24.518497	2
ACCTGGG	7000	0.0	23.476994	3
CGTATAC	230	3.6361598E-9	22.715866	3
ACATGGG	13965	0.0	22.515495	3
GTATAGG	1040	0.0	22.435574	1
GGTATCA	5930	0.0	22.323614	1
ACGCAGA	14270	0.0	21.561031	7
CGCAGAG	15035	0.0	20.432398	8
CATGGGG	10730	0.0	19.787142	4
GAGTACA	10425	0.0	19.138699	1
AGTACAT	9445	0.0	18.404871	2
>>END_MODULE