FastQCFastQC Report
Mon 15 Aug 2016
SRR2921467_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921467_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3624174
Sequences flagged as poor quality0
Sequence length98
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39890.11006645928148041No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA87800.031.5087011
GTACCTG44400.030.8946861
TACCTGG44450.029.2943952
TATCAAC107550.025.2376732
TCAACGC107400.025.1875384
CAACGCA109600.024.4727785
ATCAACG110400.024.4200343
GTACATG122250.024.2862991
AACGCAG115400.023.5957986
TACATGG126150.022.970362
ACATGGG128700.022.0164813
ACCTGGG61800.021.0701623
ACGCAGA132450.020.3104847
AGAGTAC107100.019.16579210-11
CGCAGAG139850.019.1332448
GAGTACA95700.018.9992161
CATGGGG102150.018.761864
AGTACAT89400.018.7194612
GTATACG2252.2548193E-618.4124051
GGTATCA60650.018.139181
Produced by FastQC (version 0.11.5)