##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921467_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3624174
Sequences flagged as poor quality	0
Sequence length	98
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.571569135477493	31.0	31.0	34.0	27.0	34.0
2	30.67154226038816	31.0	31.0	34.0	27.0	34.0
3	30.804826423896866	31.0	31.0	34.0	27.0	34.0
4	34.31957212871126	37.0	35.0	37.0	31.0	37.0
5	34.315460295228654	37.0	35.0	37.0	30.0	37.0
6	34.41227132030637	37.0	35.0	37.0	31.0	37.0
7	34.30502260653048	37.0	35.0	37.0	30.0	37.0
8	34.28790394721666	37.0	35.0	37.0	30.0	37.0
9	35.76077279953998	39.0	37.0	39.0	30.0	39.0
10-11	35.643943419935134	39.0	37.0	39.0	30.0	39.0
12-13	35.49008436129171	39.0	35.0	39.0	30.0	39.0
14-15	36.53457491279392	40.0	37.0	41.0	30.0	41.0
16-17	36.39438945260355	40.0	36.5	41.0	30.0	41.0
18-19	36.21286353800893	40.0	36.0	41.0	29.0	41.0
20-21	36.10064417436911	39.0	36.0	41.0	28.5	41.0
22-23	36.14666707503558	39.5	36.0	41.0	29.0	41.0
24-25	36.0140089852198	39.0	36.0	41.0	28.0	41.0
26-27	35.75496568321499	39.0	36.0	41.0	27.0	41.0
28-29	35.52875013727265	39.0	35.5	40.0	27.0	41.0
30-31	35.28602144930127	39.0	35.0	40.0	25.5	41.0
32-33	35.020790116589325	38.5	35.0	40.0	25.0	41.0
34-35	34.60650895900693	38.0	34.0	40.0	23.5	41.0
36-37	34.23068001150055	38.0	34.0	40.0	22.0	41.0
38-39	34.00080252769321	38.0	33.0	40.0	20.0	41.0
40-41	33.738281191797086	38.0	33.0	40.0	18.5	41.0
42-43	33.45354638049939	38.0	33.0	40.0	17.0	41.0
44-45	33.141796034075625	38.0	32.5	40.0	15.0	41.0
46-47	32.86409510139414	38.0	32.0	40.0	13.5	41.0
48-49	32.65596257243719	37.0	32.0	40.0	9.5	41.0
50-51	31.891056279306678	36.0	30.5	39.0	10.0	40.0
52-53	32.39617937770096	37.0	31.5	39.0	9.5	40.0
54-55	32.8325854387786	37.0	32.0	40.0	8.5	41.0
56-57	32.678700857078056	37.0	32.0	40.0	8.0	41.0
58-59	32.33863647275214	36.5	32.0	39.5	6.5	41.0
60-61	31.87926697228113	36.0	31.0	39.0	2.0	40.5
62-63	31.395919456405792	35.5	31.0	39.0	2.0	40.0
64-65	30.850352107818225	35.0	30.0	38.5	2.0	40.0
66-67	30.358383731023952	35.0	29.5	38.0	2.0	40.0
68-69	29.8366565733323	34.5	29.0	37.0	2.0	39.5
70-71	29.314457446027703	34.0	28.5	36.5	2.0	39.0
72-73	28.759937298816226	34.0	28.0	36.0	2.0	39.0
74-75	28.161786658146106	34.0	26.5	35.5	2.0	38.0
76-77	27.573383479932254	33.0	26.0	35.0	2.0	37.0
78-79	27.007048226713177	33.0	25.5	35.0	2.0	36.5
80-81	26.426001621334958	32.5	24.5	35.0	2.0	36.0
82-83	25.801279408770107	32.0	22.5	34.5	2.0	35.5
84-85	25.18238749022536	31.5	20.0	34.0	2.0	35.0
86-87	24.55778544297266	31.0	17.5	34.0	2.0	35.0
88-89	23.902635055601635	31.0	9.5	34.0	2.0	35.0
90-91	23.148303309940417	30.0	2.0	34.0	2.0	35.0
92-93	22.321220780238477	29.5	2.0	34.0	2.0	35.0
94-95	21.403330248492484	29.0	2.0	34.0	2.0	35.0
96-97	20.494676993985387	29.0	2.0	33.5	2.0	34.5
98	19.857425443701104	27.0	2.0	33.0	2.0	34.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	98168.0
3	17673.0
4	13661.0
5	12047.0
6	11232.0
7	11518.0
8	13559.0
9	15534.0
10	17468.0
11	19282.0
12	21170.0
13	24406.0
14	20985.0
15	20416.0
16	22530.0
17	25913.0
18	29042.0
19	29054.0
20	30441.0
21	33431.0
22	36963.0
23	40669.0
24	45700.0
25	51208.0
26	58079.0
27	66723.0
28	77130.0
29	90762.0
30	108307.0
31	131277.0
32	164435.0
33	211926.0
34	285701.0
35	402409.0
36	544769.0
37	568957.0
38	241563.0
39	10066.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.19718962647734	16.79163806877465	13.16554548145914	24.845626823288868
2	16.22023542506506	18.531007442713797	38.62887439478433	26.61988273743681
3	20.61754915648328	22.085376199872066	26.992259032394234	30.304815611250426
4	14.100624527573686	14.40336386861455	33.94587022790756	37.550141375904204
5	16.085306418605885	35.22748231042865	32.173042495833684	16.514168775131782
6	35.8303286724852	32.36535350632118	16.434342120775632	15.369975700417987
7	31.959258621298847	29.66445061303088	18.88677809872094	19.48951266694933
8	28.323790031914037	33.14848361411283	18.64244467479365	19.885281679179485
9	27.160102037549905	16.68317804360328	18.694815666803912	37.461904252042906
10-11	27.04454098243937	24.66426339676251	25.603186158578282	22.688009462219835
12-13	27.12262924126752	21.706346090934314	26.3315013314013	24.839523336396866
14-15	24.14776913766335	23.31916076150506	23.909791906870005	28.623278193961582
16-17	24.67181352108976	26.407165931414887	24.417556962465454	24.503463585029895
18-19	24.71242210346358	25.666857849217013	25.85532105209759	23.76539899522182
20-21	25.21477326937668	24.8952937594625	25.690250683302025	24.1996822878588
22-23	24.95315364458362	24.545639722560754	25.609382390410463	24.891824242445164
24-25	24.713990212568156	24.83207059254593	25.689751360243733	24.764187834642186
26-27	24.756387202853002	24.9624395895632	25.702228825987426	24.578944381596372
28-29	24.692967582369636	25.166606096021226	25.583756499377543	24.5566698222316
30-31	24.903990470896353	25.015746791319504	25.455304166719007	24.624958571065132
32-33	24.73133267357094	25.05044477378895	25.428885807053586	24.78933674558652
34-35	24.87589703019883	25.052830844071934	25.53857821942674	24.532693906302498
36-37	24.92741021812053	25.037776669162437	25.447566427388978	24.587246685328047
38-39	24.924310805872445	25.04536079556412	25.46365961381099	24.566668784752448
40-41	25.004723767138398	25.012645267978407	25.423758675846912	24.55887228903628
42-43	24.92773707654884	24.916308709594517	25.38681346755377	24.769140746302874
44-45	24.868835431698326	24.96764047997759	25.39292514252195	24.77059894580214
46-47	25.01840013400067	25.001331132527554	25.36384289086488	24.61642584260689
48-49	24.934413333122592	25.106944880025132	25.228024757805585	24.73061702904669
50-51	24.78695292310282	25.256816417947082	25.225519917788798	24.730710741161303
52-53	24.905839740209277	25.233117766243858	25.184545782564044	24.67649671098282
54-55	24.807067196637046	24.997801039130405	25.32926837727787	24.865863386954683
56-57	24.846660351273957	25.11396205189522	25.297987658496734	24.741389938334088
58-59	24.96528651536232	25.079960596815443	25.260186492693705	24.694566395128536
60-61	24.99623872849194	24.96205795699934	25.32734155271112	24.7143617617976
62-63	24.763139946508	25.16187528758953	25.354405989461913	24.720578776440554
64-65	24.7936502520361	25.05074219607703	25.35782385505313	24.79778369683374
66-67	24.816255302842478	25.099827392711482	25.264679076836117	24.819238227609926
68-69	24.759728294627344	25.08755149669965	25.316441126134205	24.836279082538805
70-71	24.75390826306596	24.97462778876016	25.38881648926217	24.882647458911713
72-73	24.923179304831088	24.999857951179276	25.24925410510698	24.827708638882655
74-75	24.69259134968837	25.21539319736864	25.259717460877845	24.83229799206514
76-77	24.898461228049243	24.951633191333567	25.21305854639188	24.936847034225313
78-79	24.82544556623147	24.94580595455515	25.352046514592626	24.876701964620754
80-81	24.79573701918907	25.07291889811197	25.266380875392585	24.864963207306385
82-83	24.953464215156025	25.0234948370953	25.140023131032656	24.883017816716016
84-85	24.857291931344957	25.092610269882492	25.12087340857055	24.929224390202002
86-87	24.797161416714637	25.067566678235476	25.331584850267085	24.8036870547828
88-89	24.807914979658896	24.895169342896757	25.231810978444873	25.06510469899947
90-91	24.826455620886904	24.903261600640498	25.201104742405516	25.06917803606708
92-93	24.86433602903919	25.071865706766854	25.26167402931935	24.8021242348746
94-95	24.957178309367368	24.87278898942473	25.247510826844895	24.92252187436301
96-97	24.84434439524815	24.91592643594322	25.262405128854603	24.97732403995403
98	24.958038545513595	25.002348511320932	25.014375106167954	25.025237836997523
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	450.0
1	351.0
2	441.5
3	612.0
4	485.5
5	455.5
6	769.5
7	1544.0
8	2241.5
9	2154.5
10	1687.5
11	1429.5
12	1470.0
13	1720.5
14	1987.5
15	2206.5
16	2458.0
17	2641.5
18	2645.5
19	2550.0
20	2379.5
21	2186.0
22	2075.0
23	2056.5
24	2163.5
25	2396.5
26	2791.0
27	3447.0
28	4513.5
29	6155.5
30	8674.5
31	12095.5
32	16798.0
33	22711.0
34	29754.5
35	37903.0
36	47141.0
37	57114.0
38	67012.0
39	76997.5
40	87239.5
41	97809.0
42	109202.0
43	120825.5
44	131186.0
45	141714.5
46	154339.5
47	166370.5
48	172139.5
49	171200.0
50	169653.0
51	168449.5
52	168136.5
53	168274.0
54	165915.5
55	160018.0
56	149835.5
57	139536.5
58	128673.0
59	115736.5
60	101324.0
61	84942.5
62	68432.5
63	52238.5
64	38725.0
65	29676.5
66	23027.0
67	17600.5
68	13424.0
69	10525.0
70	8049.5
71	6122.0
72	4816.5
73	3594.5
74	2734.5
75	2115.0
76	1439.5
77	870.0
78	550.0
79	348.0
80	208.5
81	113.0
82	74.5
83	45.0
84	22.0
85	17.5
86	12.0
87	10.5
88	9.5
89	8.5
90	9.0
91	8.0
92	8.0
93	5.5
94	7.0
95	6.0
96	3.0
97	4.5
98	3.5
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.4602979879001394
2	0.047045202575814515
3	0.05565406075977588
4	0.052867218847660186
5	0.06252459181043735
6	0.06349032910671508
7	0.04188540616427357
8	0.019038820983760713
9	0.011312922613538974
10-11	0.013796247089681676
12-13	0.009947094151660488
14-15	0.014016987043116583
16-17	0.013023657252659502
18-19	0.05467452721640848
20-21	0.0819910964539782
22-23	0.09827066801980258
24-25	0.0686639217653457
26-27	0.08298442624443529
28-29	0.08437784720049313
30-31	0.05539193206507193
32-33	0.03180034954171627
34-35	0.38814361562110433
36-37	0.609325048962881
38-39	0.5598792993934618
40-41	0.5534778407438495
42-43	0.527278767520544
44-45	0.5129168743001854
46-47	0.5027904289363591
48-49	0.4932710184444787
50-51	0.5060325470024343
52-53	0.587002721171776
54-55	0.5573407899289604
56-57	0.5159106599186463
58-59	0.49298129725559536
60-61	0.5061981019675104
62-63	0.518049078217547
64-65	0.5361911431404784
66-67	0.5609140179251879
68-69	0.5166556572614891
70-71	0.46578889424183273
72-73	0.4486401591093585
74-75	0.42881495204148584
76-77	0.44339758521527944
78-79	0.437175477777833
80-81	0.43356086104033636
82-83	0.45435180540448666
84-85	0.4203164638342419
86-87	0.4230343245109093
88-89	0.4506268186902726
90-91	0.4340575259355649
92-93	0.4286080083351407
94-95	0.4386516762164289
96-97	0.4339471559588475
98	0.42782162225102877
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	3624174.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.62637089783134
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.69506355424073	42.86336743957352
2	13.436984541564955	15.217753407981672
3	4.552201891877916	7.733240181938653
4	2.0412060999217414	4.623443747723373
5	1.1386048771188446	3.223753103890569
6	0.7061649870891542	2.3992536284383634
7	0.4448492217904984	1.763313791870428
8	0.3305750286087057	1.497541134364622
9	0.24344346640149084	1.2406788018994108
>10	1.3059357425324902	13.16853327161377
>50	0.07552656502895025	2.8994556641014206
>100	0.02831983540757251	2.678193848978241
>500	8.309218768477485E-4	0.32142113160988234
>1k	2.932665400021797E-4	0.37005084601597366
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3989	0.11006645928148041	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.1036997671745342E-4	0.0
5	0.0	8.277748253809006E-5	0.0	1.1036997671745342E-4	0.0
6	0.0	8.277748253809006E-5	0.0	1.1036997671745342E-4	2.7592494179363354E-5
7	0.0	8.277748253809006E-5	0.0	1.9314745925554347E-4	2.7592494179363354E-5
8	0.0	8.277748253809006E-5	0.0	1.9314745925554347E-4	2.7592494179363354E-5
9	0.0	8.277748253809006E-5	0.0	1.9314745925554347E-4	2.7592494179363354E-5
10-11	0.0	8.277748253809006E-5	0.0	2.2073995343490683E-4	5.518498835872671E-5
12-13	0.0	1.3796247089681676E-4	0.0	2.2073995343490683E-4	1.6555496507618012E-4
14-15	0.0	1.3796247089681676E-4	0.0	2.483324476142702E-4	1.6555496507618012E-4
16-17	0.0	1.3796247089681676E-4	0.0	3.173136830626785E-4	1.6555496507618012E-4
18-19	0.0	1.3796247089681676E-4	0.0	3.587024243317236E-4	1.6555496507618012E-4
20-21	0.0	1.3796247089681676E-4	0.0	3.587024243317236E-4	1.793512121658618E-4
22-23	0.0	1.3796247089681676E-4	0.0	4.1388741269045027E-4	2.345362005245885E-4
24-25	0.0	1.655549650761801E-4	0.0	4.4147990686981367E-4	2.483324476142702E-4
26-27	0.0	1.9314745925554347E-4	0.0	5.10461142318222E-4	2.483324476142702E-4
28-29	0.0	1.9314745925554347E-4	0.0	9.933297904570807E-4	2.483324476142702E-4
30-31	0.0	1.9314745925554347E-4	0.0	0.002952396877191879	2.483324476142702E-4
32-33	0.0	1.9314745925554347E-4	0.0	0.006622198603047205	2.897211888833152E-4
34-35	0.0	2.0694370634522516E-4	0.0	0.011588847555332609	3.0351743597299685E-4
36-37	0.0	3.0351743597299685E-4	0.0	0.018818081030325805	3.173136830626785E-4
38-39	0.0	3.8629491851108693E-4	0.0	0.029979244925878282	3.3110993015236025E-4
40-41	0.0	3.8629491851108693E-4	0.0	0.04196818364681166	3.3110993015236025E-4
42-43	0.0	3.8629491851108693E-4	0.0	0.05432962103916644	3.3110993015236025E-4
44-45	0.0	4.000911656007686E-4	0.0	0.06735327829182594	3.3110993015236025E-4
46-47	0.0	4.1388741269045027E-4	0.0	0.08183933773599171	3.449061772420419E-4
48-49	0.0	4.1388741269045027E-4	0.0	0.0954700298605972	3.7249867142140526E-4
50-51	0.0	4.1388741269045027E-4	0.0	0.11002507054021138	3.8629491851108693E-4
52-53	0.0	4.1388741269045027E-4	0.0	0.12498020238542631	4.1388741269045027E-4
54-55	0.0	4.27683659780132E-4	0.0	0.14032162914915233	4.1388741269045027E-4
56-57	1.3796247089681677E-5	4.4147990686981367E-4	0.0	0.1556492596657887	4.1388741269045027E-4
58-59	2.7592494179363354E-5	4.4147990686981367E-4	0.0	0.17213577493795829	4.1388741269045027E-4
60-61	2.7592494179363354E-5	4.4147990686981367E-4	0.0	0.18818081030325806	4.27683659780132E-4
62-63	2.7592494179363354E-5	4.4147990686981367E-4	0.0	0.20425343816273722	4.4147990686981367E-4
64-65	2.7592494179363354E-5	4.4147990686981367E-4	0.0	0.2217884682137226	4.5527615395949534E-4
66-67	2.7592494179363354E-5	5.242573894079037E-4	0.0	0.23914414705254217	4.828686481388587E-4
68-69	2.7592494179363354E-5	5.93238624856312E-4	0.0	0.25723102698711486	4.966648952285403E-4
70-71	2.7592494179363354E-5	6.484236132150388E-4	0.0	0.2755938318634812	4.966648952285403E-4
72-73	2.7592494179363354E-5	6.898123544840838E-4	0.0	0.294149784199103	6.34627366125357E-4
74-75	2.7592494179363354E-5	6.898123544840838E-4	0.0	0.3141957312204105	6.898123544840838E-4
76-77	2.7592494179363354E-5	7.449973428428105E-4	0.0	0.3355109329739687	7.449973428428105E-4
78-79	2.7592494179363354E-5	7.449973428428105E-4	0.0	0.35728141088148635	7.587935899324922E-4
80-81	2.7592494179363354E-5	8.277748253809007E-4	0.0	0.37947957244878416	7.863860841118555E-4
82-83	2.7592494179363354E-5	8.55367319560264E-4	0.0	0.40196745520496535	9.105523079189907E-4
84-85	2.7592494179363354E-5	8.55367319560264E-4	0.0	0.42515894656272024	9.105523079189907E-4
86	2.7592494179363354E-5	8.55367319560264E-4	0.0	0.4424456441660914	9.105523079189907E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	8780	0.0	31.508701	1
GTACCTG	4440	0.0	30.894686	1
TACCTGG	4445	0.0	29.294395	2
TATCAAC	10755	0.0	25.237673	2
TCAACGC	10740	0.0	25.187538	4
CAACGCA	10960	0.0	24.472778	5
ATCAACG	11040	0.0	24.420034	3
GTACATG	12225	0.0	24.286299	1
AACGCAG	11540	0.0	23.595798	6
TACATGG	12615	0.0	22.97036	2
ACATGGG	12870	0.0	22.016481	3
ACCTGGG	6180	0.0	21.070162	3
ACGCAGA	13245	0.0	20.310484	7
AGAGTAC	10710	0.0	19.165792	10-11
CGCAGAG	13985	0.0	19.133244	8
GAGTACA	9570	0.0	18.999216	1
CATGGGG	10215	0.0	18.76186	4
AGTACAT	8940	0.0	18.719461	2
GTATACG	225	2.2548193E-6	18.412405	1
GGTATCA	6065	0.0	18.13918	1
>>END_MODULE