FastQCFastQC Report
Mon 15 Aug 2016
SRR2921467_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921467_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3624174
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42080.11610921550676098No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG46000.031.056831
GTATCAA93650.028.221421
TACCTGG50100.027.9629172
GTACATG116150.023.9024871
TCAACGC112650.023.0185344
TATCAAC114300.022.8099462
TACATGG119000.022.5874732
CAACGCA116100.022.4073545
ATCAACG116500.022.216763
ACCTGGG62150.021.9306223
AACGCAG123900.021.1888836
ACATGGG121200.021.0417483
GAGTACA89050.020.3743951
GGTATCA66500.019.6210671
GTATACG1707.806448E-519.608431
AGTACAT84750.019.3880712
CATGGGG97500.018.8504854
ACGCAGA139900.018.6311157
AGAGTAC109900.018.03549210-11
CGCAGAG147600.017.5948338
Produced by FastQC (version 0.11.5)