##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921467_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3624174
Sequences flagged as poor quality	0
Sequence length	101
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.978351204991814	33.0	31.0	34.0	30.0	34.0
2	32.09281645969537	34.0	31.0	34.0	30.0	34.0
3	32.21643221324362	34.0	31.0	34.0	30.0	34.0
4	35.760776662489164	37.0	35.0	37.0	35.0	37.0
5	35.57044970798864	37.0	35.0	37.0	33.0	37.0
6	35.60662346785778	37.0	35.0	37.0	33.0	37.0
7	35.53910104757664	37.0	35.0	37.0	33.0	37.0
8	35.48835182858218	37.0	35.0	37.0	33.0	37.0
9	37.112634216789814	39.0	37.0	39.0	34.0	39.0
10-11	37.028318314738755	39.0	37.0	39.0	33.5	39.0
12-13	36.911822666351014	39.0	37.0	39.0	33.0	39.0
14-15	38.218795786295026	40.0	38.0	41.0	33.0	41.0
16-17	38.127279484925396	40.0	38.0	41.0	33.0	41.0
18-19	38.01826305800991	40.0	38.0	41.0	33.0	41.0
20-21	37.89954455829107	40.0	38.0	41.0	33.0	41.0
22-23	37.865649938441145	40.0	38.0	41.0	33.0	41.0
24-25	37.92131958896013	40.0	38.0	41.0	33.0	41.0
26-27	37.81590715567188	40.0	38.0	41.0	33.0	41.0
28-29	37.684766790998445	40.0	38.0	41.0	32.5	41.0
30-31	37.542230864191396	40.0	37.5	41.0	32.0	41.0
32-33	37.38482589412098	40.0	37.0	41.0	32.0	41.0
34-35	37.24312464026286	40.0	37.0	41.0	31.5	41.0
36-37	37.150555961165225	40.0	37.0	41.0	31.0	41.0
38-39	37.12062210589227	40.0	37.0	41.0	31.0	41.0
40-41	36.95668806188665	40.0	37.0	41.0	31.0	41.0
42-43	36.7175577110812	39.5	36.0	41.0	30.0	41.0
44-45	36.51269047788544	39.0	36.0	41.0	30.0	41.0
46-47	36.24841757045881	39.0	35.0	40.5	30.0	41.0
48-49	35.918018698881454	39.0	35.0	40.0	29.0	41.0
50-51	35.63750043458178	38.5	35.0	40.0	28.0	41.0
52-53	35.320666860917825	38.0	34.5	40.0	28.0	41.0
54-55	34.99029489202229	38.0	34.0	40.0	27.0	41.0
56-57	34.60555687999528	38.0	34.0	40.0	26.0	41.0
58-59	34.21716451803914	37.0	33.5	40.0	26.0	41.0
60-61	33.78357675431698	36.5	33.0	39.0	25.0	41.0
62-63	33.33643817874086	36.0	32.5	39.0	23.5	40.0
64-65	32.816669260361124	35.5	32.0	39.0	22.5	40.0
66-67	32.28280168115549	35.0	31.0	38.0	21.5	40.0
68-69	31.774080383557745	35.0	31.0	37.5	20.0	39.5
70-71	31.220931859231925	34.5	30.5	37.0	18.0	39.0
72-73	30.66895325665931	34.0	30.0	36.0	16.5	39.0
74-75	30.139721630363223	34.0	29.0	35.5	12.5	38.0
76-77	28.871106354165114	32.5	28.0	34.5	11.5	36.0
78-79	29.812990766999597	34.0	29.5	35.0	11.0	37.0
80-81	29.844525538784836	34.0	30.0	35.0	9.0	36.5
82-83	29.507212402053543	34.0	29.5	35.0	7.0	36.0
84-85	29.013778174005996	34.0	29.0	35.0	2.0	35.5
86-87	28.489646744334017	33.0	29.0	35.0	2.0	35.0
88-89	27.943049643863677	33.0	27.0	35.0	2.0	35.0
90-91	27.464087679013204	33.0	26.5	35.0	2.0	35.0
92-93	27.0174126573393	32.5	25.5	34.0	2.0	35.0
94-95	26.50808598041926	32.0	25.0	34.0	2.0	35.0
96-97	25.722419646518077	32.0	23.5	34.0	2.0	35.0
98-99	24.691859855514664	32.0	15.0	34.0	2.0	35.0
100-101	21.09676577338726	27.0	2.0	32.5	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	20696.0
3	11234.0
4	6767.0
5	5103.0
6	4725.0
7	4737.0
8	5339.0
9	6658.0
10	7831.0
11	9359.0
12	11234.0
13	13038.0
14	14439.0
15	16270.0
16	18752.0
17	21636.0
18	22486.0
19	20451.0
20	20437.0
21	21815.0
22	23568.0
23	25833.0
24	29875.0
25	35049.0
26	41571.0
27	50604.0
28	61449.0
29	74822.0
30	92363.0
31	115297.0
32	148282.0
33	197716.0
34	280866.0
35	422362.0
36	648390.0
37	780900.0
38	323537.0
39	8683.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.45088410656331	17.270730960696103	13.559824922378084	24.718560010362506
2	16.305756842800594	18.880660807124602	38.23662991898292	26.576952431091883
3	20.57790372962174	22.23953906776013	27.287883972229277	29.894673230388857
4	14.301387865084164	14.714047889213747	33.907390995856005	37.07717324984608
5	16.11462625418683	35.2127016432217	32.18800158160744	16.48467052098403
6	35.80176525742785	32.52260963006239	16.27835384002023	15.397271272489526
7	32.083505911849656	29.655063867857322	18.89107948552273	19.37035073477029
8	28.389150167534748	33.24013111102702	18.57385960778833	19.796859113649905
9	27.041072494812013	16.731522238050232	18.73159625349295	37.4958090136448
10-11	27.00064600000608	24.751632468805436	25.641753640067797	22.60596789112069
12-13	27.111043108039862	21.71000964854948	26.45336196090784	24.72558528250282
14-15	24.06369961189986	23.350671040644837	24.012331183755816	28.57329816369949
16-17	24.627171354747603	26.463236331226465	24.543302333399854	24.366289980626078
18-19	24.657995367940412	25.726190099935287	25.95824852886356	23.657566003260744
20-21	25.115964366892445	24.889258911347376	25.866888238543602	24.127888483216577
22-23	24.847144226288933	24.550955973120573	25.8755633925673	24.726336408023197
24-25	24.649813515872715	24.810276120716125	25.917408750435587	24.62250161297557
26-27	24.637356183690777	24.943941686659073	25.94704397096807	24.47165815868208
28-29	24.61640725832771	25.16309875842506	25.819178236442795	24.401315746804432
30-31	24.775614692607135	25.008640153639522	25.667071598418072	24.548673555335267
32-33	24.6049929552708	25.001062366134967	25.71091610177302	24.68302857682121
34-35	24.765594937068638	24.972001914507498	25.81873962877374	24.443663519650123
36-37	24.72476171172669	25.079512210131067	25.752725477532028	24.443000600610212
38-39	24.794639629321978	25.031694737045452	25.731890397198388	24.441775236434182
40-41	24.84039826437209	24.99080647086462	25.654272248512992	24.514523016250294
42-43	24.821656857667367	24.985989443311123	25.590906093260777	24.601447605760733
44-45	24.74357843971232	24.93821528800279	25.601548043193205	24.71665822909168
46-47	24.793393561805573	25.07544198487904	25.618894746632503	24.512269706682886
48-49	24.880528332562104	25.099149273981457	25.48091502369842	24.539407369758017
50-51	24.69437568477024	25.238632210377975	25.43649726561007	24.630494839241717
52-53	24.82929526662388	25.28012518439091	25.3139957186541	24.57658383033111
54-55	24.807693422048544	25.026565721981957	25.428038557065324	24.73770229890418
56-57	24.760472668187887	25.15725092641149	25.447808668968225	24.634467736432395
58-59	24.891474268174758	25.117679626383136	25.341939695770627	24.64890640967148
60-61	24.928938605880443	25.04985542134764	25.436902366156094	24.584303606615823
62-63	24.672884532807306	25.23041722641628	25.48416731886466	24.612530921911755
64-65	24.77426402981961	25.08403873811923	25.428913520261464	24.712783711799695
66-67	24.818606253426235	25.168516902203887	25.324709021961578	24.688167822408307
68-69	24.794605058468623	25.199424459319342	25.34416551363886	24.661804968573176
70-71	24.805459242026128	25.079348565627125	25.39625989985723	24.718932292489512
72-73	24.938928854686075	25.055364593460965	25.30091883075168	24.704787721101283
74-75	24.630355834013145	25.298778274234714	25.25880405870799	24.812061833044154
76-77	24.79130747623462	25.037996928143464	25.34393723449889	24.826758361123026
78-79	24.787493581905107	25.032288936660947	25.419162515757897	24.76105496567605
80-81	24.75684830411741	25.20138377207479	25.348251191589945	24.69351673221786
82-83	24.884927893114387	25.145321918997297	25.2790586567593	24.690691531129016
84-85	24.802531373784422	25.18760243930777	25.248676206096505	24.761189980811302
86-87	24.749800999649583	25.2248465697975	25.355662625241077	24.669689805311844
88-89	24.735752549971423	25.053050583650034	25.273823668056245	24.937373198322295
90-91	24.74640505070794	25.101513958048354	25.26383130268579	24.888249688557913
92-93	24.876173992017993	25.271326817155725	25.316944201174735	24.535554989651548
94-95	24.864196852923925	25.057262148398816	25.25591534101281	24.82262565766445
96-97	24.736302645134437	25.126535133134208	25.368988658506147	24.76817356322521
98-99	24.774551590187574	25.19111322305509	25.232574840979865	24.801760345777478
100-101	25.280083600125607	24.568878214131775	25.390130240253782	24.76090794548883
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	190.0
1	168.0
2	314.5
3	483.0
4	360.0
5	282.5
6	572.0
7	1314.5
8	1970.5
9	1886.0
10	1378.0
11	987.5
12	862.0
13	921.5
14	996.5
15	1043.0
16	1129.0
17	1193.0
18	1237.5
19	1301.5
20	1374.0
21	1467.0
22	1559.5
23	1688.0
24	1867.0
25	2156.0
26	2528.0
27	3057.5
28	3900.0
29	5282.5
30	7541.5
31	10444.0
32	14391.5
33	19469.5
34	25284.5
35	32228.5
36	40855.0
37	50154.0
38	59535.5
39	69330.5
40	79118.0
41	88688.5
42	98900.0
43	110290.0
44	122496.5
45	134676.5
46	143883.5
47	153480.5
48	166714.0
49	172536.0
50	168894.0
51	165718.5
52	165446.5
53	164901.5
54	163249.0
55	158001.5
56	147931.5
57	136610.5
58	125581.0
59	114337.0
60	100175.0
61	84547.5
62	69262.5
63	52911.0
64	38921.0
65	29456.0
66	22598.5
67	17692.0
68	13673.0
69	10479.0
70	8050.5
71	6192.5
72	4646.0
73	3435.5
74	2648.0
75	1972.0
76	1373.0
77	894.0
78	566.5
79	293.0
80	140.0
81	84.0
82	51.5
83	22.5
84	12.0
85	6.5
86	2.5
87	2.5
88	1.5
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.30760112511154264
2	0.0
3	0.005904793754383757
4	0.05695090798620596
5	3.587024243317236E-4
6	0.06252459181043735
7	0.07267862966844307
8	0.10294759578320467
9	0.09127597074533397
10-11	0.09466984752939565
12-13	0.09443531132887108
14-15	0.06344894036544603
16-17	0.034849320148535916
18-19	0.03806384572043175
20-21	0.026778515601072136
22-23	0.040781706397099035
24-25	0.0334283066982987
26-27	0.019728633338244796
28-29	0.022970751404319992
30-31	0.002938600630102197
32-33	0.005187388905720311
34-35	0.0073258072046209705
36-37	0.009919501657481126
38-39	0.015010316833573664
40-41	0.019356134666823392
42-43	0.0033386917957029657
44-45	0.013865228325130085
46-47	0.023729544994252484
48-49	0.0025661019586807918
50-51	0.006608402355957523
52-53	0.002207399534349068
54-55	0.003945726667648959
56-57	0.003021378112640287
58-59	9.243485550086722E-4
60-61	0.005380536364975853
62-63	0.014624021915062577
64-65	0.006898123544840838
66-67	0.03840875189767379
68-69	0.060386173511536696
70-71	0.0123476411452651
72-73	0.03160720208246072
74-75	0.05074259679584921
76-77	0.29590190757949264
78-79	0.07111965374730904
80-81	0.03251775439037971
82-83	0.02789601161533635
84-85	0.018956043501222625
86-87	0.11604023427131258
88-89	0.5211808263069047
90-91	0.1302641650207744
92-93	0.04556900413721857
94-95	0.007463769675517787
96-97	0.004980445199375085
98-99	0.00939524426807322
100-101	0.007063678509917019
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3624174.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.01161303153763
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.50433957371581	38.43571992589022
2	15.139263535313932	16.05113560233061
3	5.092525056216399	8.098889029006594
4	2.358720789256026	5.001583749179337
5	1.234986164974515	3.2734304338465843
6	0.8014793586261044	2.549262816735121
7	0.5361187015782755	1.9894362002926558
8	0.38811610162319815	1.645972847244633
9	0.2959275957299616	1.4118839271171029
>10	1.5231543975759552	14.459614725984594
>50	0.08941549365858979	3.2216900685589134
>100	0.034494980985989306	3.033535262543937
>500	0.001145768445674664	0.42412410431812103
>1k	3.1248229946845204E-4	0.4037213069515621
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4208	0.11610921550676098	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	2.7592494179363354E-5	0.0
5	0.0	0.0	0.0	2.7592494179363354E-5	0.0
6	0.0	0.0	0.0	2.7592494179363354E-5	0.0
7	0.0	0.0	0.0	2.7592494179363354E-5	0.0
8	0.0	0.0	0.0	2.7592494179363354E-5	2.7592494179363354E-5
9	0.0	0.0	0.0	2.7592494179363354E-5	2.7592494179363354E-5
10-11	0.0	0.0	0.0	5.518498835872671E-5	2.7592494179363354E-5
12-13	0.0	0.0	0.0	6.898123544840838E-5	9.657372962777175E-5
14-15	0.0	1.3796247089681677E-5	0.0	9.657372962777175E-5	1.3796247089681676E-4
16-17	0.0	2.7592494179363354E-5	0.0	1.5175871798649843E-4	1.6555496507618012E-4
18-19	0.0	2.7592494179363354E-5	0.0	2.2073995343490683E-4	1.9314745925554347E-4
20-21	0.0	2.7592494179363354E-5	0.0	2.345362005245885E-4	2.2073995343490683E-4
22-23	0.0	2.7592494179363354E-5	0.0	3.173136830626785E-4	2.2073995343490683E-4
24-25	0.0	2.7592494179363354E-5	0.0	3.587024243317236E-4	2.2073995343490683E-4
26-27	0.0	2.7592494179363354E-5	0.0	4.4147990686981367E-4	2.345362005245885E-4
28-29	0.0	2.7592494179363354E-5	0.0	9.38144802098354E-4	2.483324476142702E-4
30-31	0.0	2.7592494179363354E-5	0.0	0.0028972118888331523	2.483324476142702E-4
32-33	0.0	2.7592494179363354E-5	0.0	0.006567013614688477	2.483324476142702E-4
34-35	0.0	2.7592494179363354E-5	0.0	0.011740606273319108	3.0351743597299685E-4
36-37	0.0	2.7592494179363354E-5	0.0	0.01891465475995358	3.173136830626785E-4
38-39	0.0	2.7592494179363354E-5	0.0	0.030324151103120325	3.3110993015236025E-4
40-41	0.0	2.7592494179363354E-5	0.0	0.04232688607114338	3.587024243317236E-4
42-43	0.0	5.518498835872671E-5	0.0	0.05472971220476721	3.587024243317236E-4
44-45	0.0	5.518498835872671E-5	0.0	0.06775336945742672	3.7249867142140526E-4
46-47	0.0	5.518498835872671E-5	0.0	0.08234979887830993	4.000911656007686E-4
48-49	0.0	5.518498835872671E-5	0.0	0.09598049100291542	4.4147990686981367E-4
50-51	0.0	5.518498835872671E-5	0.0	0.11038377296454309	4.69072401049177E-4
52-53	0.0	5.518498835872671E-5	0.0	0.12492501739706757	5.242573894079037E-4
54-55	0.0	9.657372962777175E-5	0.0	0.14003190796026901	5.51849883587267E-4
56-57	0.0	1.1036997671745342E-4	0.0	0.15512500227638076	5.51849883587267E-4
58-59	0.0	1.3796247089681676E-4	0.0	0.17132179635966704	5.51849883587267E-4
60-61	0.0	1.3796247089681676E-4	0.0	0.18728405424242878	5.51849883587267E-4
62-63	0.0	1.3796247089681676E-4	0.0	0.20303936841884523	5.794423777666304E-4
64-65	0.0	1.3796247089681676E-4	0.0	0.22009152982169178	5.794423777666304E-4
66-67	0.0	1.3796247089681676E-4	0.0	0.23706091374200022	6.208311190356755E-4
68-69	0.0	1.3796247089681676E-4	0.0	0.25444418507499916	6.622198603047205E-4
70-71	0.0	2.2073995343490683E-4	0.0	0.2726000462450202	6.622198603047205E-4
72-73	0.0	2.759249417936335E-4	0.0	0.29060414869705486	8.415710724705822E-4
74-75	0.0	2.759249417936335E-4	0.0	0.3104431520120171	9.38144802098354E-4
76-77	0.0	3.587024243317236E-4	0.0	0.33126168887034674	9.38144802098354E-4
78-79	0.0	3.587024243317236E-4	0.0	0.3526458718593533	9.38144802098354E-4
80-81	0.0	4.0009116560076865E-4	0.0	0.37415422107216706	9.657372962777174E-4
82-83	0.0	4.4147990686981367E-4	0.0	0.3959522914738641	0.0010485147788158074
84-85	0.0	4.4147990686981367E-4	0.0	0.41859193294803176	0.0010485147788158074
86-87	0.0	4.4147990686981367E-4	0.0	0.4409556494804057	0.0011174960142642156
88-89	0.0	4.4147990686981367E-4	0.0	0.46334695850695906	0.0012002734968023059
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCTG	4600	0.0	31.05683	1
GTATCAA	9365	0.0	28.22142	1
TACCTGG	5010	0.0	27.962917	2
GTACATG	11615	0.0	23.902487	1
TCAACGC	11265	0.0	23.018534	4
TATCAAC	11430	0.0	22.809946	2
TACATGG	11900	0.0	22.587473	2
CAACGCA	11610	0.0	22.407354	5
ATCAACG	11650	0.0	22.21676	3
ACCTGGG	6215	0.0	21.930622	3
AACGCAG	12390	0.0	21.188883	6
ACATGGG	12120	0.0	21.041748	3
GAGTACA	8905	0.0	20.374395	1
GGTATCA	6650	0.0	19.621067	1
GTATACG	170	7.806448E-5	19.60843	1
AGTACAT	8475	0.0	19.388071	2
CATGGGG	9750	0.0	18.850485	4
ACGCAGA	13990	0.0	18.631115	7
AGAGTAC	10990	0.0	18.035492	10-11
CGCAGAG	14760	0.0	17.594833	8
>>END_MODULE