FastQCFastQC Report
Mon 15 Aug 2016
SRR2921466_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921466_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4350392
Sequences flagged as poor quality0
Sequence length98
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47050.10815117350344519No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA82650.036.0319631
GTACCTG53350.031.3183381
TACCTGG52400.030.7096862
TCAACGC102050.028.5721994
TATCAAC106050.027.6674332
CAACGCA105300.027.6468035
ATCAACG106550.027.2364083
AACGCAG112750.026.0167526
GGTATCA51000.024.2777611
GTACATG131550.024.142641
TACATGG138250.022.4171872
ACGCAGA130850.022.2044647
ACCTGGG69500.022.0983353
ACATGGG137850.021.4845033
CGCAGAG136800.021.1345548
CATGGGG104750.019.1261254
GTATACG3056.7539077E-918.1095621
GCAGAGT158600.017.9404479
GTGTAGC21350.017.8939721
GAGTACA104150.017.8545381
Produced by FastQC (version 0.11.5)