##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921466_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4350392
Sequences flagged as poor quality	0
Sequence length	98
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.786382928251065	31.0	31.0	34.0	28.0	34.0
2	30.896210962138586	31.0	31.0	34.0	27.0	34.0
3	31.033531690937277	33.0	31.0	34.0	28.0	34.0
4	34.551371922346306	37.0	35.0	37.0	32.0	37.0
5	34.549319463625345	37.0	35.0	37.0	32.0	37.0
6	34.64523840610225	37.0	35.0	37.0	32.0	37.0
7	34.54755617424821	37.0	35.0	37.0	32.0	37.0
8	34.53578413163687	37.0	35.0	37.0	32.0	37.0
9	36.04155073841622	39.0	37.0	39.0	32.0	39.0
10-11	35.93166535337505	39.0	37.0	39.0	31.0	39.0
12-13	35.79013121116442	39.0	37.0	39.0	30.5	39.0
14-15	36.87448993102231	40.0	37.0	41.0	31.0	41.0
16-17	36.73774995448686	40.0	37.0	41.0	30.5	41.0
18-19	36.56751506530905	40.0	37.0	41.0	30.0	41.0
20-21	36.45945365383166	40.0	37.0	41.0	30.0	41.0
22-23	36.51020816055197	40.0	37.0	41.0	30.0	41.0
24-25	36.3908301826594	40.0	37.0	41.0	30.0	41.0
26-27	36.14700916607055	39.5	36.0	41.0	29.0	41.0
28-29	35.92894192063612	39.0	36.0	41.0	28.0	41.0
30-31	35.69681651676447	39.0	35.5	40.0	27.0	41.0
32-33	35.44681904527224	39.0	35.0	40.0	26.5	41.0
34-35	35.045747026934585	38.5	35.0	40.0	25.0	41.0
36-37	34.679383145243	38.0	34.0	40.0	24.0	41.0
38-39	34.46651267288097	38.0	34.0	40.0	23.5	41.0
40-41	34.21412599140491	38.0	33.5	40.0	22.5	41.0
42-43	33.94278009889683	38.0	33.0	40.0	21.5	41.0
44-45	33.64593615931622	38.0	33.0	40.0	19.0	41.0
46-47	33.371344122552635	38.0	33.0	40.0	18.0	41.0
48-49	33.17995723144029	38.0	32.5	40.0	16.0	41.0
50-51	32.39969271734593	36.5	31.0	39.0	15.0	40.0
52-53	32.86891238766529	37.0	32.5	39.0	18.0	40.0
54-55	33.30758182251162	38.0	33.0	40.0	17.5	41.0
56-57	33.16279532970822	37.0	33.0	40.0	15.0	41.0
58-59	32.835442989965046	37.0	32.5	40.0	12.5	41.0
60-61	32.39012346473605	36.0	32.0	39.0	8.0	41.0
62-63	31.913012091783912	36.0	31.0	39.0	7.0	40.0
64-65	31.378516234858836	35.0	31.0	38.5	2.0	40.0
66-67	30.901950559857596	35.0	30.0	38.0	2.0	40.0
68-69	30.38997313345556	35.0	30.0	37.0	2.0	39.5
70-71	29.878548875595577	34.0	29.0	37.0	2.0	39.0
72-73	29.33583617292419	34.0	29.0	36.0	2.0	39.0
74-75	28.75148400420008	34.0	28.0	35.5	2.0	38.0
76-77	28.17759721422805	33.5	27.0	35.0	2.0	37.0
78-79	27.627819056305732	33.0	26.0	35.0	2.0	36.5
80-81	27.064700261493677	33.0	25.5	35.0	2.0	36.0
82-83	26.454262167639143	32.0	24.5	35.0	2.0	35.5
84-85	25.85530349908698	32.0	23.5	34.0	2.0	35.0
86-87	25.254624180993346	31.5	20.5	34.0	2.0	35.0
88-89	24.62607507553342	31.0	18.5	34.0	2.0	35.0
90-91	23.897918624344655	31.0	11.0	34.0	2.0	35.0
92-93	23.082917010696967	30.0	2.0	34.0	2.0	35.0
94-95	22.18369700937295	29.5	2.0	34.0	2.0	35.0
96-97	21.284093019663516	29.0	2.0	34.0	2.0	35.0
98	20.656311431245737	29.0	2.0	33.0	2.0	34.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	101463.0
3	18653.0
4	14828.0
5	12829.0
6	11802.0
7	12510.0
8	14396.0
9	16593.0
10	18544.0
11	20597.0
12	23443.0
13	28454.0
14	23373.0
15	22360.0
16	24896.0
17	28543.0
18	32152.0
19	31760.0
20	33153.0
21	36067.0
22	40195.0
23	44678.0
24	50062.0
25	57088.0
26	64901.0
27	74922.0
28	87236.0
29	103069.0
30	123870.0
31	151520.0
32	191697.0
33	249000.0
34	343782.0
35	488766.0
36	683102.0
37	736988.0
38	320562.0
39	12538.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.9685750294988	16.8839150295958	13.26696593890255	24.880544002002846
2	16.302959972255724	18.63939889850801	38.49224199707287	26.565399132163403
3	20.396757001171355	22.16349724272422	27.115139982028317	30.32460577407611
4	14.209131621119875	14.722648466349101	34.01980103292138	37.04841887960965
5	16.19754739092583	35.08164821402079	32.218216194396234	16.502588200657144
6	35.58009438360724	32.41354897222062	16.6769214208282	15.329435223343946
7	31.685044230102775	29.598007041034062	19.1936592519413	19.52328947692186
8	28.14765477785615	32.89911723673671	18.92310514897722	20.030122836429918
9	27.041574145191223	16.60209416741707	18.802540109625735	37.55379157776597
10-11	26.858195659728068	24.650137979178346	25.821435045527323	22.670231315566262
12-13	27.085587203092647	21.737277829553882	26.357321940649104	24.819813026704363
14-15	24.204922016422966	23.332184611479892	23.968230905507014	28.494662466590125
16-17	24.729580933641074	26.31085758084352	24.47500463529563	24.48455685021978
18-19	24.661333030358996	25.64188663163296	25.823843727798717	23.87293661020933
20-21	25.23962028843006	24.890050619160466	25.719723574246917	24.150605518162553
22-23	24.99348551852339	24.52836592184716	25.635062716768292	24.843085842861157
24-25	24.73667788068895	24.757540999651628	25.723581615566786	24.782199504092635
26-27	24.717769626027934	24.970500711456815	25.694530237202116	24.61719942531314
28-29	24.722641093370452	25.06566234216795	25.612080697646782	24.599615866814812
30-31	24.9481474053463	24.996055568869767	25.45740451371719	24.598392512066738
32-33	24.79545029677326	24.972907209866857	25.402390879806884	24.829251613552994
34-35	24.92894919581913	24.9787395685419	25.529526094886258	24.56278514075271
36-37	24.941105066710584	24.92535349103259	25.464700395084677	24.66884104717215
38-39	24.926096188812767	25.01721744377311	25.423211587877166	24.63347477953696
40-41	25.069016518654685	24.926625881691518	25.407025236628062	24.597332363025732
42-43	24.979592704588335	24.84645994669605	25.353036966093846	24.820910382621765
44-45	24.903129245695798	24.928339847227452	25.3688783558436	24.79965255123315
46-47	25.01237288621196	24.916728511843313	25.308848496723208	24.76205010522152
48-49	24.995468922352057	24.987694759810143	25.212186698650473	24.804649619187327
50-51	24.84798631180728	25.230534790341785	25.171925572185057	24.749553325665882
52-53	24.980037614614616	25.097800079225173	25.13257960731045	24.789582698849756
54-55	24.944992995745892	24.98053731685572	25.278265797774658	24.796203889623726
56-57	24.899487120590383	25.090281634794596	25.257054965989635	24.75317627862539
58-59	24.977950856892946	25.01906305090369	25.233992379862347	24.768993712341018
60-61	25.08629265806804	24.937142714545146	25.269801387960953	24.706763239425864
62-63	24.884027849030304	25.123967839768248	25.227126090625816	24.764878220575635
64-65	24.94568726426874	24.986632000200622	25.185681430539503	24.881999304991133
66-67	24.91087866887485	25.047535807765414	25.190897492465187	24.850688030894545
68-69	24.883902037722592	25.02776506800882	25.217233060188843	24.871099834079743
70-71	24.866401436630618	24.85111125225917	25.315221470929593	24.96726584018062
72-73	24.937398095461106	24.96042169441911	25.17757556884397	24.924604641275817
74-75	24.82333144699089	25.130468170799652	25.130791411854585	24.91540897035487
76-77	25.001550042829674	24.90994453213588	25.061796772701143	25.0267086523333
78-79	24.876451082825948	24.904390658068206	25.255032327358535	24.964125931747315
80-81	24.86475197486361	25.03158673467974	25.18033070018185	24.923330590274794
82-83	25.05792351544036	24.932521197437403	25.079320430289304	24.930234856832932
84-85	24.950828728835344	25.000002885807334	25.01721384074753	25.031954544609793
86-87	24.92709643771643	24.95227301428843	25.235691312314913	24.884939235680232
88-89	24.894751014269982	24.752252085812042	25.178178507822256	25.174818392095716
90-91	24.944213185118024	24.83608564449313	25.164716711269563	25.054984459119282
92-93	25.00139106720136	24.9576388290839	25.17383722366537	24.867132880049372
94-95	25.018758451982926	24.807603918475806	25.152108272203122	25.021529357338146
96-97	25.027055233776384	24.74016700704579	25.179619737448206	25.053158021729622
98	24.971301202994464	24.896933684768534	24.982591397472103	25.149173714764895
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	593.0
1	470.5
2	561.5
3	746.0
4	592.0
5	548.0
6	901.5
7	1822.0
8	2604.0
9	2486.0
10	2019.5
11	1789.0
12	1937.5
13	2257.5
14	2608.0
15	2956.0
16	3204.0
17	3349.0
18	3392.0
19	3206.5
20	2933.5
21	2710.5
22	2503.0
23	2425.5
24	2521.5
25	2737.0
26	3109.5
27	3819.5
28	4848.5
29	6440.5
30	9073.5
31	12880.5
32	18322.0
33	25060.0
34	33280.0
35	43694.5
36	55147.0
37	67389.0
38	80183.0
39	92624.5
40	105041.0
41	117978.5
42	131531.5
43	145226.5
44	158171.5
45	170636.0
46	184553.0
47	197660.0
48	204564.5
49	205258.0
50	204543.0
51	204134.0
52	204115.5
53	204744.0
54	201831.5
55	193622.0
56	181664.0
57	168216.0
58	153746.0
59	137251.5
60	120440.0
61	102234.5
62	82939.0
63	64146.5
64	47654.5
65	36081.5
66	27716.0
67	21379.0
68	16663.5
69	12924.0
70	9846.5
71	7586.0
72	5925.5
73	4414.0
74	3512.0
75	2690.0
76	1821.0
77	1141.5
78	697.5
79	429.5
80	260.5
81	171.5
82	90.5
83	53.0
84	41.5
85	29.5
86	22.0
87	19.0
88	12.5
89	9.0
90	8.5
91	6.5
92	6.0
93	5.0
94	5.0
95	5.5
96	4.0
97	2.0
98	2.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.47248615756924894
2	0.048455403559035604
3	0.05610988618956637
4	0.05369631058534495
5	0.06383332812307489
6	0.06470681262745978
7	0.043283455835704004
8	0.019952224994897013
9	0.011516203597285026
10-11	0.014745797620076537
12-13	0.010711678395877888
14-15	0.014481453625328475
16-17	0.014148150327602662
18-19	0.05491459160461862
20-21	0.08318790582549802
22-23	0.09896809299024087
24-25	0.06913170123519903
26-27	0.08452111901640127
28-29	0.08556700177823055
30-31	0.05723622147153636
32-33	0.033307343338255496
34-35	0.39594133126394127
36-37	0.6209785233146806
38-39	0.5709140693528307
40-41	0.5658340673668028
42-43	0.5403650981336854
44-45	0.5251825582614165
46-47	0.5144364002140497
48-49	0.5045867131053937
50-51	0.5189072616904408
52-53	0.5978541703828069
54-55	0.5702129831058902
56-57	0.5278030117745711
58-59	0.5055291569127564
60-61	0.5179418314487522
62-63	0.5300671755556741
64-65	0.5477322503351422
66-67	0.573936785466689
68-69	0.52896382670803
70-71	0.4785086033626395
72-73	0.460935474320475
74-75	0.44269573868285894
76-77	0.45665999753585423
78-79	0.4509363753887006
80-81	0.44731601198236853
82-83	0.4672797301944285
84-85	0.4332598073920695
86-87	0.43639745567755733
88-89	0.4639581904343333
90-91	0.4458333869683468
92-93	0.44099474254274096
94-95	0.4523155614482557
96-97	0.44678732399287235
98	0.44133953905762974
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	4350392.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.92549160485992
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.27012153275406	41.53593058271964
2	14.115885351802726	15.78875655474817
3	4.946456440661475	8.298990244380558
4	2.1896570576421146	4.898305895787454
5	1.227156394070879	3.431466230723056
6	0.7284724419546833	2.444410766214507
7	0.4879471978792335	1.9102080843026998
8	0.34092271955496245	1.5253016552301653
9	0.2478827784241976	1.2476669619376632
>10	1.3446512907408796	13.44078915627431
>50	0.07539848799133222	2.8727285344922904
>100	0.024911776849483258	2.207708585872726
>500	3.714437280209742E-4	0.14031121221819384
>1k	1.6508594606437247E-4	0.2574255350985176
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4705	0.10815117350344519	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	2.2986434325918217E-5	0.0	0.0	0.0
4	0.0	2.2986434325918217E-5	0.0	0.0	0.0
5	0.0	2.2986434325918217E-5	0.0	0.0	0.0
6	0.0	2.2986434325918217E-5	0.0	0.0	2.2986434325918217E-5
7	0.0	2.2986434325918217E-5	0.0	0.0	2.2986434325918217E-5
8	0.0	2.2986434325918217E-5	0.0	0.0	2.2986434325918217E-5
9	0.0	2.2986434325918217E-5	0.0	0.0	2.2986434325918217E-5
10-11	0.0	2.2986434325918217E-5	0.0	0.0	2.2986434325918217E-5
12-13	0.0	2.2986434325918217E-5	0.0	4.5972868651836435E-5	4.5972868651836435E-5
14-15	0.0	2.2986434325918217E-5	0.0	6.895930297775465E-5	1.2642538879255018E-4
16-17	0.0	2.2986434325918217E-5	0.0	1.0343895446663197E-4	1.379186059555093E-4
18-19	0.0	2.2986434325918217E-5	0.0	1.1493217162959108E-4	2.0687790893326395E-4
20-21	0.0	4.5972868651836435E-5	0.0	1.2642538879255018E-4	2.5285077758510036E-4
22-23	0.0	4.5972868651836435E-5	0.0	1.609050402814275E-4	2.643439947480595E-4
24-25	0.0	4.5972868651836435E-5	0.0	2.2986434325918215E-4	2.758372119110186E-4
26-27	0.0	4.5972868651836435E-5	0.0	3.5628973205173236E-4	3.103168633998959E-4
28-29	0.0	4.5972868651836435E-5	0.0	6.4362016112571E-4	3.333032977258141E-4
30-31	0.0	4.5972868651836435E-5	0.0	0.0023101366497547808	3.5628973205173236E-4
32-33	0.0	4.5972868651836435E-5	0.0	0.005976472924738736	3.792761663776506E-4
34-35	0.0	4.5972868651836435E-5	0.0	0.010654212310063093	3.9076938354060966E-4
36-37	0.0	6.895930297775465E-5	0.0	0.01817077633463835	3.9076938354060966E-4
38-39	0.0	1.0343895446663197E-4	0.0	0.031479921809345	3.9076938354060966E-4
40-41	0.0	1.1493217162959108E-4	0.0	0.04537522135936256	3.9076938354060966E-4
42-43	0.0	1.1493217162959108E-4	0.0	0.059155588737750536	4.022626007035688E-4
44-45	0.0	1.1493217162959108E-4	0.0	0.07471740477639716	4.367422521924461E-4
46-47	0.0	1.1493217162959108E-4	0.0	0.09057804446128073	4.827151208442825E-4
48-49	0.0	1.1493217162959108E-4	0.0	0.10609388763127553	5.057015551702007E-4
50-51	0.0	1.1493217162959108E-4	0.0	0.12187407479601839	5.516744238220372E-4
52-53	0.0	1.1493217162959108E-4	0.0	0.13918285984343481	6.091405096368327E-4
54-55	0.0	1.2642538879255018E-4	0.0	0.15735363617807313	7.125794641034647E-4
56-57	0.0	1.8389147460734574E-4	0.0	0.17598414119922987	7.47059115592342E-4
58-59	0.0	2.2986434325918215E-4	0.0	0.1959823390627787	7.930319842441784E-4
60-61	0.0	2.4135756042214124E-4	0.0	0.2152104913764093	8.045252014071375E-4
62-63	0.0	2.5285077758510036E-4	0.0	0.23473746733627682	8.50498070058974E-4
64-65	0.0	2.5285077758510036E-4	0.0	0.2543104161647962	8.964709387108104E-4
66-67	0.0	2.758372119110186E-4	0.0	0.27486028845216703	9.65430241688565E-4
68-69	0.0	2.758372119110186E-4	0.0	0.2960078080320118	0.0010114031103404014
70-71	0.0	2.873304290739777E-4	0.0	0.3186609390602042	0.0010803624133181563
72-73	0.0	3.103168633998959E-4	0.0	0.34065895671010793	0.001172308150621829
74-75	0.0	3.21810080562855E-4	0.0	0.3635879249502114	0.0012182810192736654
76-77	0.0	3.21810080562855E-4	0.0	0.3871720065686035	0.0012412674535995836
78-79	0.0	3.21810080562855E-4	0.0	0.411468667651099	0.0012412674535995836
80-81	0.0	3.21810080562855E-4	0.0	0.4368571843640757	0.00128724032225142
82-83	0.0	3.21810080562855E-4	0.0	0.4622457010770523	0.0013447064080662156
84-85	0.0	3.4479651488877324E-4	0.0	0.48824335829966586	0.0013561996252291748
86	0.0	3.4479651488877324E-4	0.0	0.5096782083085846	0.001379186059555093
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	8265	0.0	36.031963	1
GTACCTG	5335	0.0	31.318338	1
TACCTGG	5240	0.0	30.709686	2
TCAACGC	10205	0.0	28.572199	4
TATCAAC	10605	0.0	27.667433	2
CAACGCA	10530	0.0	27.646803	5
ATCAACG	10655	0.0	27.236408	3
AACGCAG	11275	0.0	26.016752	6
GGTATCA	5100	0.0	24.277761	1
GTACATG	13155	0.0	24.14264	1
TACATGG	13825	0.0	22.417187	2
ACGCAGA	13085	0.0	22.204464	7
ACCTGGG	6950	0.0	22.098335	3
ACATGGG	13785	0.0	21.484503	3
CGCAGAG	13680	0.0	21.134554	8
CATGGGG	10475	0.0	19.126125	4
GTATACG	305	6.7539077E-9	18.109562	1
GCAGAGT	15860	0.0	17.940447	9
GTGTAGC	2135	0.0	17.893972	1
GAGTACA	10415	0.0	17.854538	1
>>END_MODULE