FastQCFastQC Report
Mon 15 Aug 2016
SRR2921466_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921466_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4350392
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA80600.036.4020731
TCAACGC100350.028.5879784
GTACCTG54250.028.0073091
TATCAAC103700.027.5718942
CAACGCA103850.027.564485
TACCTGG54650.027.2890452
ATCAACG106150.027.0253183
AACGCAG112700.025.570476
GTACATG128200.024.7438131
GGTATCA50900.023.5810531
TACATGG132650.023.2373352
ACGCAGA128200.022.3688077
ACATGGG131850.022.2979223
GTATAGG9900.021.168881
CGCAGAG138800.020.5921158
ACCTGGG72750.019.7816963
AGAGTAC120700.018.4080410-11
CATGGGG103100.017.9206944
GCAGAGT158600.017.8109769
GAGTACA100550.017.6688041
Produced by FastQC (version 0.11.5)