##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2921466_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4350392 Sequences flagged as poor quality 0 Sequence length 101 %GC 49 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.03098594333568 33.0 31.0 34.0 30.0 34.0 2 32.151219936042544 34.0 31.0 34.0 30.0 34.0 3 32.274974301166424 34.0 31.0 34.0 30.0 34.0 4 35.80506308397037 37.0 35.0 37.0 35.0 37.0 5 35.62176098153913 37.0 35.0 37.0 35.0 37.0 6 35.65282232037941 37.0 35.0 37.0 35.0 37.0 7 35.59036289143599 37.0 35.0 37.0 33.0 37.0 8 35.54454518121585 37.0 35.0 37.0 33.0 37.0 9 37.182938457040194 39.0 37.0 39.0 34.0 39.0 10-11 37.09892234998593 39.0 37.0 39.0 34.0 39.0 12-13 36.98719736060566 39.0 37.0 39.0 33.0 39.0 14-15 38.31448074104587 40.0 38.0 41.0 34.0 41.0 16-17 38.22491007706891 40.0 38.0 41.0 33.0 41.0 18-19 38.11655271524957 40.0 38.0 41.0 33.0 41.0 20-21 38.00238576201869 40.0 38.0 41.0 33.0 41.0 22-23 37.968605357862 40.0 38.0 41.0 33.0 41.0 24-25 38.027347995307096 40.0 38.0 41.0 33.0 41.0 26-27 37.92680452704032 40.0 38.0 41.0 33.0 41.0 28-29 37.797064609350144 40.0 38.0 41.0 33.0 41.0 30-31 37.66369306490081 40.0 38.0 41.0 33.0 41.0 32-33 37.51082753002488 40.0 37.5 41.0 32.0 41.0 34-35 37.37580935235262 40.0 37.0 41.0 32.0 41.0 36-37 37.290794599659066 40.0 37.0 41.0 32.0 41.0 38-39 37.27005439969548 40.0 37.0 41.0 32.0 41.0 40-41 37.114281655538164 40.0 37.0 41.0 31.0 41.0 42-43 36.88946984547599 40.0 36.5 41.0 31.0 41.0 44-45 36.69311788454925 39.5 36.0 41.0 30.5 41.0 46-47 36.44254920016403 39.0 36.0 41.0 30.0 41.0 48-49 36.12988737566637 39.0 35.0 40.0 30.0 41.0 50-51 35.863029124731746 39.0 35.0 40.0 29.0 41.0 52-53 35.55551844523436 38.0 35.0 40.0 28.0 41.0 54-55 35.2313283492614 38.0 34.5 40.0 28.0 41.0 56-57 34.85456735852769 38.0 34.0 40.0 27.0 41.0 58-59 34.47346124211336 37.0 34.0 40.0 26.0 41.0 60-61 34.05363758024564 37.0 33.0 39.5 26.0 41.0 62-63 33.61522892649674 36.0 33.0 39.0 25.5 40.0 64-65 33.10643810948531 35.5 32.0 39.0 23.5 40.0 66-67 32.58168769618922 35.0 32.0 38.0 22.5 40.0 68-69 32.08735132374278 35.0 31.0 37.5 21.5 39.5 70-71 31.544258885176326 35.0 31.0 37.0 20.0 39.0 72-73 31.005609034772036 34.0 30.0 36.0 18.0 39.0 74-75 30.49053418634459 34.0 30.0 36.0 17.5 38.0 76-77 29.184862191728932 32.5 28.0 34.5 15.0 36.0 78-79 30.12721945516634 34.0 30.0 35.0 17.0 37.0 80-81 30.165521750683617 34.0 30.0 35.0 16.5 36.5 82-83 29.84066964540207 34.0 30.0 35.0 11.5 36.0 84-85 29.368077290506235 34.0 29.0 35.0 7.0 35.5 86-87 28.86341506696408 34.0 29.0 35.0 2.0 35.0 88-89 28.336418534237833 33.0 28.5 35.0 2.0 35.0 90-91 27.87951878819196 33.0 27.0 35.0 2.0 35.0 92-93 27.464348844885702 33.0 27.0 34.5 2.0 35.0 94-95 26.987988208878647 33.0 25.5 34.0 2.0 35.0 96-97 26.23675188350843 32.0 24.5 34.0 2.0 35.0 98-99 25.244178915371307 32.0 21.5 34.0 2.0 35.0 100-101 21.624621298494482 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 23914.0 3 12925.0 4 7711.0 5 5891.0 6 5244.0 7 5442.0 8 6147.0 9 7491.0 10 8762.0 11 10474.0 12 12451.0 13 14457.0 14 16126.0 15 18012.0 16 20251.0 17 23406.0 18 24056.0 19 22208.0 20 22702.0 21 24012.0 22 26070.0 23 28934.0 24 33210.0 25 39312.0 26 46807.0 27 57213.0 28 68988.0 29 84694.0 30 105359.0 31 131200.0 32 169976.0 33 229722.0 34 329730.0 35 503786.0 36 790183.0 37 981742.0 38 420553.0 39 11231.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.19111363429879 17.319087981412448 13.65258472753032 24.837213656758443 2 16.41810669015574 18.91650223703979 38.07776402678195 26.587627046022522 3 20.392087628350527 22.258688825242828 27.326182691474376 30.023040854932265 4 14.408320265547827 14.955327263502216 33.99012659613666 36.6462258748133 5 16.2378794820028 35.014622890783336 32.26938576043285 16.478111866781017 6 35.717683407061735 32.46787570201459 16.546980693218703 15.267460197704974 7 31.806806248396896 29.540456886130436 19.184662649369052 19.46807421610362 8 28.214930354527805 32.945580529274295 18.91505304984854 19.924436066349358 9 26.989296940753764 16.522373162604683 18.86104153618972 37.627288360451836 10-11 26.870015408136133 24.630227166754043 25.889994444419518 22.609762980690302 12-13 27.04469430307894 21.69631545693129 26.47880790648724 24.780182333502527 14-15 24.125319710448917 23.364363125974915 24.038441964826358 28.471875198749807 16-17 24.73822791644587 26.24304300971297 24.58872599366235 24.430003080178814 18-19 24.68792800383381 25.65485448904448 25.863676668484842 23.793540838636865 20-21 25.223781314438444 24.885804274527764 25.745542998202854 24.14487141283094 22-23 24.984799377073415 24.492250901803974 25.72619193810472 24.796757783017892 24-25 24.745857066926288 24.726127856093573 25.815913816000734 24.712101260979402 26-27 24.701949587900213 24.94436055098689 25.770974232957318 24.582715628155587 28-29 24.696214180484432 25.026265585042268 25.733203274762907 24.54431695971039 30-31 24.938543874869033 24.925130877295093 25.532129358764465 24.604195889071406 32-33 24.75746757575618 24.92543918126773 25.496167939422637 24.820925303553448 34-35 24.88512373487258 24.968089128142683 25.63068583920507 24.51610129777967 36-37 24.82744272468596 24.96646787047845 25.58870741097707 24.617381993858526 38-39 24.89308517046139 24.93743281182898 25.572312174289678 24.597169843419948 40-41 25.058328087560643 24.882102312569575 25.48932828206424 24.57024131780554 42-43 24.918349745371657 24.907143523769374 25.408642054202712 24.765864676656257 44-45 24.870289133645095 24.877300997834254 25.475321054294703 24.777088814225944 46-47 24.916647985606964 24.895357325562014 25.467561058325145 24.720433630505884 48-49 24.915258406117854 24.974461437460175 25.355746889168618 24.754533267253358 50-51 24.849018029562732 25.136401864276625 25.303811597212988 24.710768508947655 52-53 24.972544993741234 25.039092136070174 25.201667149583606 24.786695720604985 54-55 24.972334732496286 24.90347614603399 25.337523159748244 24.786665961721475 56-57 24.939474631526245 25.064099882259423 25.259985536436822 24.736439949777505 58-59 24.984745439923813 25.014306357999498 25.262631861865465 24.738316340211227 60-61 25.057515072994953 24.95748436150913 25.296655482414 24.688345083081924 62-63 24.89230736337734 25.111204321798187 25.271086577465645 24.725401737358823 64-65 24.943435448942367 24.925401457454548 25.246105364715927 24.885057728887155 66-67 24.942663655639073 25.02450455385155 25.202788477381016 24.830043313128357 68-69 24.894677518795202 25.029437853439685 25.24854127515385 24.827343352611262 70-71 24.899166584767716 24.873775035934962 25.312017766037968 24.915040613259354 72-73 24.99609968929728 24.928394664305976 25.201456652515947 24.874048993880795 74-75 24.83731913759853 25.119208456081598 25.122623693398623 24.920848712921245 76-77 24.968721947595817 24.92709473033552 25.14847636430582 24.955706957762843 78-79 24.85820549437607 24.938821789794478 25.275043154849534 24.92792956097992 80-81 24.81650297631879 25.09174420041792 25.214522021309925 24.877230801953363 82-83 25.02528067411452 24.963717231693757 25.151603569598997 24.859398524592727 84-85 24.95902731883764 25.043438456601606 25.065659174196288 24.931875050364468 86-87 24.87389111750225 25.01635693019486 25.238887125789915 24.870864826512978 88-89 24.831443965957995 24.884893846065502 25.207730659745526 25.075931528230978 90-91 24.8965893205536 24.952899417599617 25.18610354728293 24.96440771456385 92-93 24.957365321157482 25.06781130329824 25.182442848947495 24.792380526596784 94-95 24.982491461821844 24.9539743548335 25.1127896983474 24.950744484997255 96-97 24.939987680880318 24.90520725901656 25.184140265556454 24.970664794546668 98-99 24.811866042875348 24.981430859463273 25.154478467498198 25.052224630163185 100-101 25.352255461371982 24.49861490877915 25.21185556707132 24.937274062777544 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 240.0 1 204.5 2 388.0 3 581.5 4 408.0 5 319.5 6 636.5 7 1531.5 8 2252.5 9 2021.5 10 1443.0 11 1051.0 12 920.0 13 985.0 14 1060.5 15 1079.5 16 1149.0 17 1240.5 18 1318.0 19 1398.5 20 1451.0 21 1441.5 22 1501.5 23 1655.5 24 1800.5 25 2021.0 26 2355.5 27 2859.0 28 3741.5 29 4845.5 30 6687.0 31 9805.5 32 14210.5 33 19983.0 34 27055.5 35 35881.5 36 46542.5 37 57941.0 38 69898.0 39 81999.5 40 94466.0 41 107161.0 42 120213.5 43 133066.5 44 146270.0 45 159909.5 46 172077.5 47 184510.0 48 198049.0 49 205679.0 50 203715.5 51 202252.5 52 203569.5 53 202633.5 54 199904.0 55 193060.0 56 180974.5 57 165925.5 58 151615.0 59 136679.0 60 119344.5 61 102352.5 62 84690.0 63 65128.5 64 48082.5 65 36336.5 66 27941.5 67 21991.5 68 17082.0 69 13099.5 70 10138.5 71 7810.0 72 5804.0 73 4466.5 74 3482.0 75 2608.5 76 1857.0 77 1131.5 78 689.0 79 386.5 80 204.0 81 116.5 82 61.0 83 27.5 84 20.0 85 15.5 86 4.5 87 1.0 88 0.5 89 0.5 90 1.0 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.31551179755755343 2 0.0 3 0.006735025257494037 4 0.05861540753109145 5 4.8271512084428254E-4 6 0.0667526052824665 7 0.07716546003210745 8 0.10730067543338623 9 0.09557759392716794 10-11 0.09910601159619639 12-13 0.09861180325818913 14-15 0.06674111206530355 16-17 0.037203543956498636 18-19 0.039444721303275664 20-21 0.02942263593717532 22-23 0.04335241513868175 24-25 0.034721009049299464 26-27 0.022066976952881488 28-29 0.024583991511569533 30-31 0.0030801821996730414 32-33 0.005792581450131391 34-35 0.008861270432641472 36-37 0.01056226657275942 38-39 0.015354938129713369 40-41 0.019722360651637832 42-43 0.002988236462369368 44-45 0.014286068933558172 46-47 0.024526525425754734 48-49 0.002482534907199167 50-51 0.006585613434375568 52-53 0.002126245175147435 54-55 0.003953666704057933 56-57 0.0034479651488877323 58-59 0.0012297742364366245 60-61 0.0052409070263093535 62-63 0.0144469739738396 64-65 0.006286789788138633 66-67 0.03931829591448311 68-69 0.06145423217034235 70-71 0.011941452632314513 72-73 0.031709786152604176 74-75 0.051294228198286496 76-77 0.30078898637180285 78-79 0.07484383016518971 80-81 0.034008429585196 82-83 0.028043449877620225 84-85 0.019481003091215688 86-87 0.1181272860008937 88-89 0.5280788489864822 90-91 0.13103416887489677 92-93 0.04855884251350223 94-95 0.008539460352078618 96-97 0.005597196758361086 98-99 0.009953126063122588 100-101 0.007298192898479034 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4350392.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.69853312130276 #Duplication Level Percentage of deduplicated Percentage of total 1 71.26030888742646 37.55313748138308 2 15.602709392879325 16.444797954454252 3 5.548024429438153 8.7711824745763 4 2.5355631113296124 5.344818264142281 5 1.3414754421645014 3.534689401016012 6 0.820686987201611 2.594940022635976 7 0.5797174564931671 2.138518170740179 8 0.3800780242505274 1.6023643479716583 9 0.2900619933161807 1.3757257405803462 >10 1.5197458293936514 14.447396748626023 >50 0.08977297658150908 3.2127443432449048 >100 0.031243867258854374 2.578253729109349 >500 3.9317296447473994E-4 0.13260567398252746 >1k 2.1842930204000433E-4 0.2688256475371073 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 2.2986434325918217E-5 0.0 3 0.0 0.0 0.0 2.2986434325918217E-5 0.0 4 0.0 0.0 0.0 2.2986434325918217E-5 0.0 5 0.0 0.0 0.0 2.2986434325918217E-5 2.2986434325918217E-5 6 0.0 0.0 0.0 2.2986434325918217E-5 6.895930297775465E-5 7 0.0 0.0 0.0 2.2986434325918217E-5 6.895930297775465E-5 8 0.0 0.0 0.0 2.2986434325918217E-5 6.895930297775465E-5 9 0.0 0.0 0.0 2.2986434325918217E-5 9.194573730367287E-5 10-11 0.0 0.0 0.0 2.2986434325918217E-5 9.194573730367287E-5 12-13 0.0 0.0 0.0 3.4479651488877324E-5 1.0343895446663197E-4 14-15 0.0 2.2986434325918217E-5 0.0 4.5972868651836435E-5 1.379186059555093E-4 16-17 0.0 6.895930297775465E-5 0.0 4.5972868651836435E-5 1.609050402814275E-4 18-19 0.0 1.1493217162959108E-4 0.0 4.5972868651836435E-5 2.0687790893326395E-4 20-21 0.0 1.1493217162959108E-4 0.0 5.7466085814795545E-5 2.0687790893326395E-4 22-23 0.0 1.379186059555093E-4 0.0 6.895930297775465E-5 2.2986434325918215E-4 24-25 0.0 1.609050402814275E-4 0.0 1.379186059555093E-4 2.5285077758510036E-4 26-27 0.0 1.609050402814275E-4 0.0 2.643439947480595E-4 2.758372119110186E-4 28-29 0.0 1.609050402814275E-4 0.0 5.28687989496119E-4 2.758372119110186E-4 30-31 0.0 1.609050402814275E-4 0.0 0.0021377383923103944 2.873304290739777E-4 32-33 0.0 1.609050402814275E-4 0.0 0.005769595015805472 3.103168633998959E-4 34-35 0.0 1.609050402814275E-4 0.0 0.010504800486944625 3.677829492146915E-4 36-37 0.0 2.1837112609622306E-4 0.0 0.01794091199137917 3.677829492146915E-4 38-39 0.0 2.643439947480595E-4 0.0 0.031227071031759895 4.0226260070356883E-4 40-41 0.0 2.758372119110186E-4 0.0 0.04506490449596266 4.597286865183643E-4 42-43 0.0 2.9882364623693683E-4 0.0 0.058856765091513594 4.597286865183643E-4 44-45 0.0 2.9882364623693683E-4 0.0 0.07447604721597502 4.827151208442825E-4 46-47 0.0 2.9882364623693683E-4 0.0 0.09009532934043644 5.057015551702007E-4 48-49 0.0 2.9882364623693683E-4 0.0 0.10555370642461645 5.057015551702007E-4 50-51 0.0 2.9882364623693683E-4 0.0 0.12125344106921859 5.057015551702007E-4 52-53 0.0 2.9882364623693683E-4 0.0 0.13841281429351654 6.32126943962751E-4 54-55 0.0 3.792761663776506E-4 0.0 0.15639969915354757 6.895930297775465E-4 56-57 0.0 3.9076938354060966E-4 0.0 0.1748807923515858 7.125794641034647E-4 58-59 0.0 4.137558178665279E-4 0.0 0.194499714048757 7.35565898429383E-4 60-61 0.0 4.367422521924461E-4 0.0 0.213612934190758 7.585523327553011E-4 62-63 0.0 4.597286865183643E-4 0.0 0.23271466111559602 8.045252014071375E-4 64-65 0.0 4.827151208442825E-4 0.0 0.25189684056057476 8.045252014071375E-4 66-67 0.0 5.057015551702007E-4 0.0 0.272067436681568 8.160184185700967E-4 68-69 0.0 5.28687989496119E-4 0.0 0.2926058157517759 8.275116357330558E-4 70-71 0.0 5.401812066590781E-4 0.0 0.3148222045277759 8.390048528960148E-4 72-73 0.0 5.516744238220372E-4 0.0 0.33630302740534646 9.884166760144833E-4 74-75 0.0 5.631676409849963E-4 0.0 0.3588871991305611 0.0010803624133181563 76-77 0.0 5.861540753109146E-4 0.0 0.38219544353704216 0.0010918556304811153 78-79 0.0 6.091405096368327E-4 0.0 0.40581400480692315 0.0011033488476440744 80-81 0.0 6.4362016112571E-4 0.0 0.4304669556214704 0.0011033488476440744 82-83 0.0 6.4362016112571E-4 0.0 0.455315291127788 0.0011263352819699925 84-85 0.0 6.551133782886692E-4 0.0 0.48086471288104615 0.0011263352819699925 86-87 0.0 6.895930297775465E-4 0.0 0.5083564883348444 0.0012067878021107064 88-89 0.0 6.895930297775465E-4 0.0 0.5367217482930273 0.00128724032225142 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 8060 0.0 36.402073 1 TCAACGC 10035 0.0 28.587978 4 GTACCTG 5425 0.0 28.007309 1 TATCAAC 10370 0.0 27.571894 2 CAACGCA 10385 0.0 27.56448 5 TACCTGG 5465 0.0 27.289045 2 ATCAACG 10615 0.0 27.025318 3 AACGCAG 11270 0.0 25.57047 6 GTACATG 12820 0.0 24.743813 1 GGTATCA 5090 0.0 23.581053 1 TACATGG 13265 0.0 23.237335 2 ACGCAGA 12820 0.0 22.368807 7 ACATGGG 13185 0.0 22.297922 3 GTATAGG 990 0.0 21.16888 1 CGCAGAG 13880 0.0 20.592115 8 ACCTGGG 7275 0.0 19.781696 3 AGAGTAC 12070 0.0 18.40804 10-11 CATGGGG 10310 0.0 17.920694 4 GCAGAGT 15860 0.0 17.810976 9 GAGTACA 10055 0.0 17.668804 1 >>END_MODULE