FastQCFastQC Report
Mon 15 Aug 2016
SRR2921465_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921465_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3337930
Sequences flagged as poor quality0
Sequence length98
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34370.10296800711818402No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG45250.038.2522471
TACCTGG46700.036.226182
GTATCAA68500.033.4677471
ACCTGGG57500.028.3854123
TCAACGC80350.027.9020424
TATCAAC84750.026.886222
CAACGCA83150.026.6867775
ATCAACG86050.026.2669143
AACGCAG89350.025.0851486
GGTATCA45750.024.2501091
GTACATG92550.024.2236881
TACATGG97400.022.2175462
ACGCAGA103100.021.8223517
ACATGGG98100.020.7971613
CCTGGGG58700.020.697644
CGCAGAG109200.020.4321218
GAGTACA74650.019.3636651
TATAACG2402.0548396E-719.1030122
AGTACAT71900.018.2368662
CATGGGG74550.017.7731024
Produced by FastQC (version 0.11.5)