##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921465_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3337930
Sequences flagged as poor quality	0
Sequence length	98
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.561920411752194	31.0	31.0	34.0	27.0	34.0
2	30.677077110664396	31.0	31.0	34.0	27.0	34.0
3	30.80599683037092	31.0	31.0	34.0	27.0	34.0
4	34.32413801367914	37.0	35.0	37.0	31.0	37.0
5	34.31794645184291	37.0	35.0	37.0	31.0	37.0
6	34.41541733948885	37.0	35.0	37.0	31.0	37.0
7	34.314208506469576	37.0	35.0	37.0	31.0	37.0
8	34.296850443238775	37.0	35.0	37.0	30.0	37.0
9	35.77355187196856	39.0	37.0	39.0	30.0	39.0
10-11	35.65204078575644	39.0	37.0	39.0	30.0	39.0
12-13	35.49893646661254	39.0	35.0	39.0	30.0	39.0
14-15	36.537527599440374	40.0	37.0	41.0	30.0	41.0
16-17	36.39631028811269	40.0	36.5	41.0	30.0	41.0
18-19	36.218831431456024	40.0	36.0	41.0	29.0	41.0
20-21	36.10871408327915	39.0	36.0	41.0	29.0	41.0
22-23	36.160517296647924	39.5	36.0	41.0	29.0	41.0
24-25	36.041881795004684	39.0	36.0	41.0	28.0	41.0
26-27	35.78203047996813	39.0	36.0	41.0	27.0	41.0
28-29	35.55624159284347	39.0	35.5	40.0	27.0	41.0
30-31	35.30870269897811	39.0	35.0	40.0	26.0	41.0
32-33	35.042581330345456	38.5	35.0	40.0	25.0	41.0
34-35	34.62892915669292	38.0	34.0	40.0	23.5	41.0
36-37	34.25866210495727	38.0	34.0	40.0	22.5	41.0
38-39	34.03238204515972	38.0	33.5	40.0	20.5	41.0
40-41	33.77817674426965	38.0	33.0	40.0	18.5	41.0
42-43	33.49763026786062	38.0	33.0	40.0	17.5	41.0
44-45	33.182433723894746	38.0	32.5	40.0	15.0	41.0
46-47	32.8947966254535	38.0	32.0	40.0	13.5	41.0
48-49	32.68174137863886	37.0	32.0	40.0	9.0	41.0
50-51	31.929504812863065	36.0	30.5	39.0	10.0	40.0
52-53	32.442767074204674	37.0	31.5	39.0	9.0	40.0
54-55	32.88316831089927	38.0	33.0	40.0	8.5	41.0
56-57	32.7389756525751	37.0	32.0	40.0	8.0	41.0
58-59	32.414299131497664	37.0	32.0	40.0	6.5	41.0
60-61	31.97117210366904	36.0	31.0	39.0	2.0	41.0
62-63	31.491803602831695	35.5	31.0	39.0	2.0	40.0
64-65	30.9501018595357	35.0	30.0	38.5	2.0	40.0
66-67	30.46430302612697	35.0	29.5	38.0	2.0	40.0
68-69	29.943744026986785	34.5	29.0	37.5	2.0	39.5
70-71	29.426577998939464	34.0	28.5	37.0	2.0	39.0
72-73	28.870875063287727	34.0	28.0	36.0	2.0	39.0
74-75	28.274441944558454	34.0	26.5	35.5	2.0	38.0
76-77	27.683790103447347	33.0	26.0	35.0	2.0	37.0
78-79	27.11426497859452	33.0	25.5	35.0	2.0	37.0
80-81	26.52665229648315	32.5	24.5	35.0	2.0	36.0
82-83	25.89694691021082	32.0	22.5	34.5	2.0	35.5
84-85	25.27277908763815	31.5	20.0	34.0	2.0	35.0
86-87	24.644847105841045	31.0	18.0	34.0	2.0	35.0
88-89	23.989946014446076	31.0	11.0	34.0	2.0	35.0
90-91	23.23298286662692	30.0	4.0	34.0	2.0	35.0
92-93	22.39715032969535	30.0	2.0	34.0	2.0	35.0
94-95	21.469964319203818	29.0	2.0	34.0	2.0	35.0
96-97	20.56301195651197	29.0	2.0	33.5	2.0	34.5
98	19.927120700553935	27.0	2.0	33.0	2.0	34.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	90645.0
3	16192.0
4	12422.0
5	10651.0
6	9990.0
7	10484.0
8	11716.0
9	13772.0
10	15674.0
11	17260.0
12	19614.0
13	22776.0
14	18841.0
15	18959.0
16	21209.0
17	24633.0
18	28014.0
19	26777.0
20	27743.0
21	29711.0
22	32961.0
23	36617.0
24	41143.0
25	46633.0
26	52606.0
27	60366.0
28	70571.0
29	82946.0
30	98878.0
31	119840.0
32	151640.0
33	193649.0
34	262561.0
35	367787.0
36	501871.0
37	528674.0
38	231634.0
39	10467.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.587106418614844	17.289013767032692	13.598429136235646	24.525450678116815
2	15.820967816385487	18.725028429748697	39.45821321502242	25.9957905388434
3	20.490221675593023	22.63287690861004	27.089290440860182	29.787610974936758
4	14.08652889252322	14.965762294040205	34.78322514274203	36.16448367069454
5	15.88133843804926	35.183148321856116	32.41986258517228	16.515650654922343
6	34.57504644395353	33.058411909078075	17.004527629693072	15.362014017275321
7	31.135848195914832	29.740588297176302	19.45037795469579	19.673185552213077
8	27.607363066188306	33.618208519527	19.02976431799706	19.744664096287636
9	26.608663553378825	17.11937778986925	19.277655697850083	36.99430295890184
10-11	26.65343391185811	25.12277802156183	25.83693628516951	22.386851781410552
12-13	26.775852235742914	22.079471542005468	26.528036429013476	24.616639793238143
14-15	23.846498539513146	23.60632759281174	24.279723120996806	28.267450746678303
16-17	24.326475186684892	26.698561353275952	24.733643311254546	24.24132014878461
18-19	24.256754478193002	25.997987126904682	26.166645808525256	23.57861258637706
20-21	24.78516193063414	25.24657653362598	26.130096219869447	23.838165315870434
22-23	24.513730466073216	24.954963519476124	26.0452856918794	24.48602032257126
24-25	24.30002674216832	25.171200843277703	26.113562476540643	24.415209938013334
26-27	24.34428033503666	25.344703718518353	26.059283282529318	24.251732663915675
28-29	24.31098435561065	25.4313942350435	25.996546657233466	24.26107475211239
30-31	24.48391145462225	25.347638659031592	25.90020044921968	24.268249437126478
32-33	24.3208710543946	25.360960156939395	25.8544375731514	24.46373121551461
34-35	24.471140881659213	25.381207045729802	25.964099979591154	24.183552093019827
36-37	24.57861647734424	25.354160834896888	25.81592394603433	24.25129874172454
38-39	24.456312774284374	25.427705840592342	25.82762918259188	24.288352202531403
40-41	24.643019206304917	25.274797623000563	25.815338407849087	24.266844762845434
42-43	24.52361802687584	25.23069786216266	25.790475674079964	24.455208436881534
44-45	24.492276360201803	25.345402063271532	25.748743848751648	24.413577727775014
46-47	24.586561945690047	25.349292440755654	25.70963720084202	24.354508412712274
48-49	24.61127336598104	25.39136145637321	25.563452996425372	24.43391218122037
50-51	24.438200301465617	25.599746419531115	25.55600228282655	24.406050996176717
52-53	24.544110488344863	25.604685265256727	25.47540642656591	24.3757978198325
54-55	24.489558547750207	25.40435231387017	25.6687804140904	24.437308724289224
56-57	24.474454711467015	25.549188990889792	25.619020741099053	24.35733555654414
58-59	24.58497081072031	25.425448017125422	25.582784362483668	24.4067968096706
60-61	24.653533510959807	25.314799104097606	25.67944836409271	24.352219020849876
62-63	24.428034922041967	25.510707981434464	25.648161827770195	24.413095268753366
64-65	24.502477314194657	25.39052361985364	25.656548889618264	24.450450176333437
66-67	24.47665134000671	25.462515626367804	25.599384301898187	24.4614487317273
68-69	24.476519682100943	25.448113850427568	25.59490113356167	24.48046533390982
70-71	24.448902802820296	25.299588286709472	25.644591771965658	24.606917138504578
72-73	24.539846740239017	25.36471768814006	25.561202245907587	24.534233325713338
74-75	24.41295191414021	25.52274713060655	25.505157843911558	24.55914311134169
76-77	24.54241735341622	25.30683457952237	25.52554906983138	24.625198997230026
78-79	24.45340213513921	25.282346323757114	25.649779506985265	24.61447203411841
80-81	24.448424274643596	25.499038255072843	25.487692629240442	24.56484484104312
82-83	24.597741525150788	25.385778639846222	25.44748395812226	24.568995876880734
84-85	24.5720285732039	25.36820444955178	25.439849596345343	24.61991738089897
86-87	24.518763636823206	25.379348549904112	25.59279840325608	24.509089410016603
88-89	24.438400216231738	25.23890010162952	25.539048053620895	24.78365162851785
90-91	24.51067724093558	25.23352279583943	25.524156695233003	24.73164326799199
92-93	24.52429104262584	25.35921264290077	25.56728852702767	24.549207787445724
94-95	24.65096324813797	25.237486441814465	25.468637704817382	24.64291260523018
96-97	24.589167130240636	25.183916107381037	25.554479358406603	24.672437403971724
98	24.523755070597154	25.222534516171745	25.438508853232545	24.815201559998556
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	402.0
1	277.0
2	257.5
3	357.5
4	305.5
5	285.0
6	440.0
7	839.5
8	1250.5
9	1330.0
10	1225.5
11	1222.0
12	1394.0
13	1665.0
14	1924.5
15	2204.5
16	2452.5
17	2607.5
18	2599.5
19	2513.0
20	2415.0
21	2248.0
22	2240.0
23	2425.0
24	2661.5
25	3096.0
26	3867.5
27	4872.5
28	6179.0
29	7979.5
30	10624.5
31	14278.5
32	19339.5
33	25321.5
34	32428.5
35	41290.5
36	50930.0
37	60736.0
38	70122.5
39	78333.5
40	86687.0
41	96383.5
42	106669.5
43	116568.5
44	125367.0
45	134477.5
46	144910.5
47	154681.0
48	159114.5
49	158151.5
50	157074.0
51	155066.5
52	153427.0
53	152918.5
54	149933.5
55	142836.0
56	131607.5
57	120407.5
58	109303.0
59	97032.0
60	84377.5
61	70228.0
62	56047.5
63	42759.0
64	31048.0
65	23015.5
66	17475.0
67	13125.0
68	9827.0
69	7438.0
70	5517.0
71	4066.0
72	3142.0
73	2334.5
74	1721.0
75	1287.0
76	865.5
77	517.0
78	318.5
79	186.0
80	115.0
81	71.0
82	43.0
83	29.5
84	18.5
85	9.0
86	5.0
87	6.5
88	8.5
89	7.5
90	6.0
91	5.0
92	4.5
93	4.5
94	3.5
95	3.5
96	6.0
97	4.0
98	1.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.47742163556455647
2	0.049012411884011946
3	0.05779030716641751
4	0.05518390139996945
5	0.06563948315273238
6	0.06557956577879104
7	0.042511376811377115
8	0.020341948453083198
9	0.012223144284032319
10-11	0.015114157576701728
12-13	0.01109969352263229
14-15	0.015443703133379071
16-17	0.014619839241685714
18-19	0.05699640196169482
20-21	0.0856818447361089
22-23	0.10218908125694667
24-25	0.07112192286836452
26-27	0.08664052271917026
28-29	0.08689517155842094
30-31	0.05761055504459351
32-33	0.032969535011219527
34-35	0.4011917565676932
36-37	0.6282186864314111
38-39	0.5780229064120578
40-41	0.5729299296270444
42-43	0.545847276605561
44-45	0.5293400400847231
46-47	0.5200828058107869
48-49	0.5099867283016719
50-51	0.5238276416821204
52-53	0.6080115520696959
54-55	0.576959373024599
56-57	0.5334743388866753
58-59	0.5098668935537893
60-61	0.5236478895602964
62-63	0.5364552282402566
64-65	0.5544903577965985
66-67	0.5818126803138473
68-69	0.5338488224738086
70-71	0.48172070714484727
72-73	0.46530334668492146
74-75	0.44592007621489965
76-77	0.45937152666472936
78-79	0.45384414891864117
80-81	0.45128268118264914
82-83	0.46999188119583096
84-85	0.43759156123705406
86-87	0.43940406179877944
88-89	0.46641181810283616
90-91	0.4500094369863958
92-93	0.44529094378851564
94-95	0.45579146357173456
96-97	0.4498446642080571
98	0.44488650151441167
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	3337930.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.49835864213051
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.56701156198383	45.55611514071068
2	13.637095881670186	16.227696432094675
3	4.3116547584919145	7.696091434854016
4	1.819336839760488	4.329902231316394
5	0.9566786029588683	2.8460403312049567
6	0.5838904780348555	2.084431504190572
7	0.40467254565580624	1.6854146577837164
8	0.2859694564915981	1.3611770626425794
9	0.21420843259931183	1.1470545132266248
>10	1.1323069961670578	12.11835882897448
>50	0.06615836884554703	2.6427514334439657
>100	0.02051150945937641	1.94198299528844
>500	3.0274073086112034E-4	0.1094864443632382
>1k	2.0182715032311265E-4	0.25349698990561936
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3437	0.10296800711818402	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.995868697066745E-5
2	0.0	0.0	0.0	0.0	2.995868697066745E-5
3	0.0	2.995868697066745E-5	0.0	0.0	2.995868697066745E-5
4	0.0	2.995868697066745E-5	0.0	2.995868697066745E-5	2.995868697066745E-5
5	0.0	5.99173739413349E-5	0.0	2.995868697066745E-5	2.995868697066745E-5
6	8.987606091200235E-5	5.99173739413349E-5	0.0	2.995868697066745E-5	2.995868697066745E-5
7	8.987606091200235E-5	5.99173739413349E-5	0.0	2.995868697066745E-5	2.995868697066745E-5
8	8.987606091200235E-5	5.99173739413349E-5	0.0	5.99173739413349E-5	2.995868697066745E-5
9	8.987606091200235E-5	5.99173739413349E-5	0.0	5.99173739413349E-5	5.99173739413349E-5
10-11	8.987606091200235E-5	5.99173739413349E-5	0.0	5.99173739413349E-5	8.987606091200235E-5
12-13	8.987606091200235E-5	5.99173739413349E-5	0.0	5.99173739413349E-5	1.198347478826698E-4
14-15	8.987606091200235E-5	5.99173739413349E-5	0.0	5.99173739413349E-5	1.198347478826698E-4
16-17	8.987606091200235E-5	5.99173739413349E-5	0.0	5.99173739413349E-5	1.198347478826698E-4
18-19	8.987606091200235E-5	5.99173739413349E-5	0.0	7.489671742666862E-5	1.797521218240047E-4
20-21	8.987606091200235E-5	5.99173739413349E-5	0.0	8.987606091200235E-5	2.0971080879467216E-4
22-23	8.987606091200235E-5	5.99173739413349E-5	0.0	8.987606091200235E-5	2.546488392506733E-4
24-25	8.987606091200235E-5	5.99173739413349E-5	0.0	1.0485540439733606E-4	2.995868697066745E-4
26-27	8.987606091200235E-5	5.99173739413349E-5	0.0	1.6477277833867095E-4	2.995868697066745E-4
28-29	8.987606091200235E-5	5.99173739413349E-5	0.0	3.4452490016267565E-4	3.2954555667734196E-4
30-31	8.987606091200235E-5	5.99173739413349E-5	0.0	0.0010785127309440282	3.2954555667734196E-4
32-33	8.987606091200235E-5	5.99173739413349E-5	0.0	0.003280476223288086	3.595042436480094E-4
34-35	8.987606091200235E-5	8.987606091200235E-5	0.0	0.006081613455045492	3.595042436480094E-4
36-37	8.987606091200235E-5	1.4979343485333724E-4	0.0	0.010485540439733609	3.595042436480094E-4
38-39	8.987606091200235E-5	1.797521218240047E-4	0.0	0.01864928263924049	3.595042436480094E-4
40-41	8.987606091200235E-5	1.797521218240047E-4	0.0	0.028251041813339407	3.595042436480094E-4
42-43	8.987606091200235E-5	1.797521218240047E-4	0.0	0.037403420682878316	3.595042436480094E-4
44-45	1.0485540439733606E-4	1.797521218240047E-4	0.0	0.04799381652700925	3.7448358713334314E-4
46-47	1.198347478826698E-4	1.797521218240047E-4	0.0	0.05792512125778551	4.1942161758934426E-4
48-49	1.198347478826698E-4	1.797521218240047E-4	0.0	0.06915962887178581	4.4938030456001174E-4
50-51	1.198347478826698E-4	1.797521218240047E-4	0.0	0.07986985946379942	4.793389915306792E-4
52-53	1.3481409136800352E-4	1.797521218240047E-4	0.0	0.09068494546021036	5.692150524426816E-4
54-55	1.6477277833867095E-4	3.145662131920082E-4	0.0	0.10242875075271202	6.590911133546839E-4
56-57	1.797521218240047E-4	3.595042436480094E-4	0.0	0.11526604811964301	6.740704568400176E-4
58-59	1.797521218240047E-4	3.894629306186768E-4	0.0	0.12793857270823536	6.890498003253513E-4
60-61	1.797521218240047E-4	3.894629306186768E-4	0.0	0.1413750438145797	6.890498003253513E-4
62-63	1.797521218240047E-4	3.894629306186768E-4	0.0	0.1548864116383507	7.04029143810685E-4
64-65	1.797521218240047E-4	3.894629306186768E-4	0.0	0.16854757289697508	7.190084872960188E-4
66-67	1.797521218240047E-4	3.894629306186768E-4	0.0	0.18283786658198342	7.190084872960188E-4
68-69	2.0971080879467216E-4	4.194216175893443E-4	0.0	0.1979220654717145	7.190084872960188E-4
70-71	2.0971080879467216E-4	4.643596480453455E-4	0.0	0.21365037613131493	7.339878307813525E-4
72-73	2.0971080879467216E-4	5.092976785013466E-4	0.0	0.22952848022576866	7.939052047226875E-4
74-75	2.546488392506733E-4	5.092976785013466E-4	0.0	0.24542156366370776	8.388432351786886E-4
76-77	2.6962818273600705E-4	5.092976785013466E-4	0.0	0.26282756079366554	8.388432351786886E-4
78-79	2.6962818273600705E-4	5.242770219866803E-4	0.0	0.2811023598457727	8.388432351786886E-4
80-81	2.6962818273600705E-4	5.392563654720141E-4	0.0	0.29810391470162645	8.388432351786886E-4
82-83	2.6962818273600705E-4	5.392563654720141E-4	0.0	0.3151803662749069	9.28719296090691E-4
84-85	2.6962818273600705E-4	5.392563654720141E-4	0.0	0.3345336780579581	9.28719296090691E-4
86	2.6962818273600705E-4	5.99173739413349E-4	0.0	0.3494381248258651	9.28719296090691E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCTG	4525	0.0	38.252247	1
TACCTGG	4670	0.0	36.22618	2
GTATCAA	6850	0.0	33.467747	1
ACCTGGG	5750	0.0	28.385412	3
TCAACGC	8035	0.0	27.902042	4
TATCAAC	8475	0.0	26.88622	2
CAACGCA	8315	0.0	26.686777	5
ATCAACG	8605	0.0	26.266914	3
AACGCAG	8935	0.0	25.085148	6
GGTATCA	4575	0.0	24.250109	1
GTACATG	9255	0.0	24.223688	1
TACATGG	9740	0.0	22.217546	2
ACGCAGA	10310	0.0	21.822351	7
ACATGGG	9810	0.0	20.797161	3
CCTGGGG	5870	0.0	20.69764	4
CGCAGAG	10920	0.0	20.432121	8
GAGTACA	7465	0.0	19.363665	1
TATAACG	240	2.0548396E-7	19.103012	2
AGTACAT	7190	0.0	18.236866	2
CATGGGG	7455	0.0	17.773102	4
>>END_MODULE