FastQCFastQC Report
Mon 15 Aug 2016
SRR2921465_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921465_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3337930
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33780.10120044458691464No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG44650.035.2009661
GTATCAA71700.033.27981
TACCTGG46900.032.608562
TCAACGC82600.027.8304584
CAACGCA85550.026.9155
ACCTGGG54600.026.8794823
TATCAAC89050.026.027622
ATCAACG88950.025.7368953
AACGCAG92050.025.3250276
GGTATCA48300.024.5535531
GTACATG96500.023.8880231
TACATGG100500.022.6842122
ACATGGG99750.022.0456623
ACGCAGA106650.021.5919887
CGCAGAG112800.020.3308858
AGTACAT72000.020.119442
GAGTACA76750.019.7338071
GTGTAGC20300.019.7081261
CATGGGG76850.019.6534274
GTATAAG8950.018.6254651
Produced by FastQC (version 0.11.5)