##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921465_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3337930
Sequences flagged as poor quality	0
Sequence length	101
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.00494198500268	33.0	31.0	34.0	30.0	34.0
2	32.13125350142154	34.0	31.0	34.0	30.0	34.0
3	32.251842908629	34.0	31.0	34.0	30.0	34.0
4	35.77715260655556	37.0	35.0	37.0	35.0	37.0
5	35.59532075268205	37.0	35.0	37.0	33.0	37.0
6	35.63048655903509	37.0	35.0	37.0	35.0	37.0
7	35.56796427726166	37.0	35.0	37.0	33.0	37.0
8	35.519512092823994	37.0	35.0	37.0	33.0	37.0
9	37.15526688696288	39.0	37.0	39.0	34.0	39.0
10-11	37.06810538267729	39.0	37.0	39.0	34.0	39.0
12-13	36.955650358156106	39.0	37.0	39.0	33.0	39.0
14-15	38.27684373249289	40.0	38.0	41.0	34.0	41.0
16-17	38.191212517937764	40.0	38.0	41.0	33.0	41.0
18-19	38.089206933638515	40.0	38.0	41.0	33.0	41.0
20-21	37.978328335225726	40.0	38.0	41.0	33.0	41.0
22-23	37.94955151845605	40.0	38.0	41.0	33.0	41.0
24-25	38.01120559748107	40.0	38.0	41.0	33.0	41.0
26-27	37.915113558402965	40.0	38.0	41.0	33.0	41.0
28-29	37.7877112461915	40.0	38.0	41.0	33.0	41.0
30-31	37.65299242344807	40.0	38.0	41.0	32.5	41.0
32-33	37.498482442711506	40.0	37.5	41.0	32.0	41.0
34-35	37.366462298490376	40.0	37.0	41.0	32.0	41.0
36-37	37.27580806068431	40.0	37.0	41.0	31.5	41.0
38-39	37.24120083404985	40.0	37.0	41.0	32.0	41.0
40-41	37.08239208131986	40.0	37.0	41.0	31.0	41.0
42-43	36.858402363141224	40.0	36.5	41.0	31.0	41.0
44-45	36.662421470791784	39.5	36.0	41.0	30.0	41.0
46-47	36.40009511883113	39.0	36.0	41.0	30.0	41.0
48-49	36.086868957707324	39.0	35.0	40.0	29.5	41.0
50-51	35.819936757211806	39.0	35.0	40.0	28.5	41.0
52-53	35.5183228827447	38.5	35.0	40.0	28.0	41.0
54-55	35.20682563744596	38.0	34.5	40.0	27.5	41.0
56-57	34.84055507455219	38.0	34.0	40.0	26.5	41.0
58-59	34.46642140488267	37.5	34.0	40.0	26.0	41.0
60-61	34.053381586791815	37.0	33.0	39.5	26.0	41.0
62-63	33.6240419960874	36.0	33.0	39.0	24.5	40.5
64-65	33.11818402423059	36.0	32.0	39.0	23.0	40.0
66-67	32.60507110694353	35.0	32.0	38.5	22.0	40.0
68-69	32.109152978043284	35.0	31.0	38.0	21.0	40.0
70-71	31.56203515352329	35.0	31.0	37.0	19.5	39.0
72-73	31.021246700799598	34.0	30.0	36.5	18.0	39.0
74-75	30.499421497754597	34.0	30.0	36.0	16.0	38.5
76-77	29.200219597175497	32.5	28.5	34.5	13.0	36.0
78-79	30.126814972153397	34.0	30.0	35.0	14.5	37.0
80-81	30.16125952311762	34.0	30.0	35.0	14.0	37.0
82-83	29.82844202844278	34.0	30.0	35.0	9.0	36.0
84-85	29.345266827045503	34.0	29.0	35.0	6.5	36.0
86-87	28.839833669369938	34.0	29.0	35.0	2.0	35.0
88-89	28.304002330785845	33.0	28.5	35.0	2.0	35.0
90-91	27.845570458337953	33.0	27.0	35.0	2.0	35.0
92-93	27.425865731156733	33.0	26.5	34.5	2.0	35.0
94-95	26.952278058557248	33.0	25.5	34.0	2.0	35.0
96-97	26.20275005767047	32.0	24.5	34.0	2.0	35.0
98-99	25.209816862546546	32.0	21.0	34.0	2.0	35.0
100-101	21.579920489644778	28.0	2.0	32.5	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	19169.0
3	10063.0
4	5977.0
5	4584.0
6	3993.0
7	4068.0
8	4950.0
9	5735.0
10	6838.0
11	8263.0
12	9746.0
13	11569.0
14	12623.0
15	14362.0
16	16778.0
17	20002.0
18	20401.0
19	17719.0
20	17472.0
21	18563.0
22	20046.0
23	22233.0
24	25392.0
25	29765.0
26	35706.0
27	43222.0
28	53200.0
29	64434.0
30	79335.0
31	99948.0
32	128790.0
33	172503.0
34	245958.0
35	374307.0
36	593525.0
37	759375.0
38	345971.0
39	11345.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.78242211554558	17.725717730706616	14.003709205724475	24.488150948023335
2	15.99221673312502	18.902912883134157	39.2025297115278	25.902340672213022
3	20.578713108005594	22.637456272492926	27.36853584906565	29.41529477043583
4	14.229213585536405	15.192496138326595	34.74157139007353	35.83671888606347
5	15.959094224987755	35.06901763526034	32.51245163522738	16.45943650452453
6	34.730512354933296	33.079330761830846	16.894333671184032	15.295823212051825
7	31.276600720265655	29.70993897049741	19.3931767840846	19.620283525152335
8	27.731885301064914	33.62833211970859	18.981101043557334	19.65868153566917
9	26.663753955250197	17.10479283964774	19.222246078334944	37.009207126767116
10-11	26.702711326298832	25.113958411645925	25.890949285826636	22.292380976228607
12-13	26.823611155676698	22.00977850522137	26.627459282967592	24.539151056134344
14-15	23.829450262447168	23.585724696834475	24.380965542960904	28.203859497757456
16-17	24.34560245991917	26.64468883131362	24.852840909942323	24.156867798824884
18-19	24.26887233882179	25.97416629066122	26.255634649484755	23.501326721032235
20-21	24.749994793197022	25.16432145767708	26.297906145990364	23.78777760313553
22-23	24.51679478758632	24.862546686322016	26.21510317783939	24.405555348252275
24-25	24.29817418748301	25.08272243651853	26.2880080413311	24.33109533466736
26-27	24.32669683962982	25.264442928870995	26.22295490476459	24.1859053267346
28-29	24.27091934560478	25.42645979821081	26.145827667854377	24.156793188330035
30-31	24.467864075793802	25.2726047385956	26.036330920649263	24.223200264961335
32-33	24.31115313540371	25.23653644728548	26.03795908144164	24.41435133586917
34-35	24.42315873995554	25.323250420953602	26.14410994529101	24.109480893799848
36-37	24.51327126056302	25.29302598163704	25.98710505910256	24.206597698697383
38-39	24.436774483419985	25.3590175485066	25.996503572459634	24.20770439561378
40-41	24.57053433845006	25.238657790116587	25.99457788318702	24.196229988246337
42-43	24.494770758068935	25.158797675066918	25.95674447651631	24.389687090347838
44-45	24.473791013364014	25.276233968407162	25.89383428913265	24.356140729096175
46-47	24.50885425071309	25.28920958943169	25.915717368187867	24.28621879166736
48-49	24.516697392611363	25.36251346865931	25.730056385241777	24.390732753487544
50-51	24.436356380420907	25.580646330725052	25.64335412707482	24.33964316177922
52-53	24.545931852890444	25.542635571823503	25.57290952790754	24.338523047378516
54-55	24.509125270407097	25.350519947096757	25.741721734266505	24.398633048229645
56-57	24.491709371877626	25.53170421879049	25.683345791620543	24.29324061771134
58-59	24.61198518344534	25.42365112310539	25.647490269296036	24.316873424153236
60-61	24.65332398367815	25.359955982119008	25.728064949444	24.25865508475884
62-63	24.47252876819581	25.48713305933839	25.7104649579262	24.3298732145396
64-65	24.517230236988507	25.375457632643624	25.742687277906136	24.364624852461738
66-67	24.560939487670698	25.41564584982038	25.636200731616416	24.387213930892504
68-69	24.501033770433718	25.46650071181322	25.636442558666513	24.39602295908655
70-71	24.48216272092945	25.333123593148986	25.71615411409117	24.468559571830394
72-73	24.601258268003953	25.307726633439316	25.614606649646415	24.476408448910323
74-75	24.43568387866057	25.5525211305686	25.456302781459655	24.55549220931118
76-77	24.505998872211123	25.344643476125988	25.615562224391525	24.533795427271365
78-79	24.532681980298708	25.294640908360456	25.71418377797552	24.458493333365315
80-81	24.46687184380564	25.532626176972702	25.56888855656699	24.431613422654667
82-83	24.58524114465383	25.467726560179855	25.506309824662665	24.440722470503655
84-85	24.5421781605622	25.47777094412528	25.524606018292044	24.455444877020476
86-87	24.501273203017053	25.47643318387439	25.59782201973086	24.424471593377696
88-89	24.46241623726381	25.35313443377934	25.596943389605624	24.587505939351225
90-91	24.521987315993634	25.375467557926036	25.576642900716852	24.52590222536348
92-93	24.540116079447895	25.496234619143443	25.63595738268376	24.327691918724902
94-95	24.565267706467786	25.4138868353029	25.510346702739874	24.51049875548944
96-97	24.507969293077462	25.398000269043596	25.60366282785194	24.490367610026997
98-99	24.48823087564107	25.440280394020952	25.537775921481042	24.533712808856933
100-101	24.917487942273915	24.979596691608037	25.630854715860714	24.472060650257333
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	118.0
1	94.5
2	144.0
3	233.0
4	198.5
5	157.0
6	273.5
7	614.5
8	969.0
9	1000.5
10	760.0
11	583.0
12	567.5
13	634.5
14	725.0
15	776.5
16	854.0
17	961.0
18	1079.0
19	1196.5
20	1294.5
21	1431.0
22	1564.0
23	1784.5
24	2121.0
25	2462.0
26	2943.0
27	3806.5
28	4936.0
29	6461.0
30	8808.5
31	11707.0
32	15823.0
33	21203.5
34	27521.5
35	34886.0
36	43966.5
37	53891.5
38	62699.0
39	71167.5
40	79707.0
41	88265.5
42	97860.5
43	107625.0
44	117516.0
45	127617.0
46	136364.5
47	145182.0
48	154821.0
49	158975.5
50	155411.5
51	152519.0
52	152017.5
53	150479.0
54	147803.5
55	141489.0
56	131035.0
57	119174.5
58	107900.0
59	96718.0
60	83685.5
61	70341.0
62	57023.5
63	43199.0
64	31208.5
65	22949.0
66	17404.0
67	13310.0
68	10140.0
69	7574.0
70	5640.0
71	4111.0
72	2987.5
73	2331.0
74	1759.5
75	1232.0
76	919.5
77	577.0
78	318.0
79	173.5
80	98.0
81	48.0
82	27.0
83	16.5
84	7.5
85	4.5
86	1.0
87	0.5
88	1.0
89	1.0
90	1.0
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.31555484986204024
2	0.0
3	0.006411159011722834
4	0.05814981141006552
5	4.493803045600118E-4
6	0.06402171405631635
7	0.07471696530484462
8	0.10683267773740011
9	0.09353102072242378
10-11	0.0979798857375679
12-13	0.09793494770711189
14-15	0.06623865689214573
16-17	0.0369989784087743
18-19	0.03956044614476637
20-21	0.028445773278648744
22-23	0.04294577777245179
24-25	0.035021705068710246
26-27	0.02175000674070457
28-29	0.024970565590051317
30-31	0.0025315090490213997
32-33	0.005332646280778806
34-35	0.008088845482080212
36-37	0.010245870943968267
38-39	0.01568337262914441
40-41	0.02014721698777386
42-43	0.003400310971170755
44-45	0.01487448808093639
46-47	0.025779450138259344
48-49	0.0026513437969040692
50-51	0.00680062194234151
52-53	0.0023217982402267273
54-55	0.004149278145437442
56-57	0.003744835871333431
58-59	0.0012582648527680329
60-61	0.005856923302765486
62-63	0.015593496568232407
64-65	0.007085229468562853
66-67	0.04030941331903305
68-69	0.062403944959900294
70-71	0.014005686158787034
72-73	0.03358368809411821
74-75	0.05296695856414005
76-77	0.3049644540179093
78-79	0.07531613904425796
80-81	0.034107965116104894
82-83	0.02968905878793144
84-85	0.020596597292333874
86-87	0.12029910753071514
88-89	0.5366349803620807
90-91	0.13539828576393154
92-93	0.05034557345420665
94-95	0.00816374219950688
96-97	0.005422522341690808
98-99	0.010215912256997601
100-101	0.007070250125077518
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3337930.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.24137056558597
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.81740838281128	41.515922190488816
2	15.036825073178084	16.91383302141006
3	4.879184179671971	8.232360165200278
4	2.071478257815509	4.660111052654258
5	1.1080822821887768	3.11600331248696
6	0.6837729791340357	2.3073797701327186
7	0.45175660470030676	1.7785187427280622
8	0.3153427892393009	1.4188248531834398
9	0.24276214120483192	1.2287947988516452
>10	1.2863353709260756	13.077053899483197
>50	0.0816016662306371	3.123417122589436
>100	0.024864667968187176	2.2430976558731954
>500	3.72657684028421E-4	0.13326789261674812
>1k	2.1294724701736257E-4	0.25141552230119385
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3378	0.10120044458691464	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.995868697066745E-5
2	0.0	0.0	0.0	5.99173739413349E-5	2.995868697066745E-5
3	0.0	0.0	0.0	5.99173739413349E-5	2.995868697066745E-5
4	0.0	0.0	0.0	8.987606091200235E-5	2.995868697066745E-5
5	0.0	0.0	0.0	8.987606091200235E-5	2.995868697066745E-5
6	2.995868697066745E-5	0.0	0.0	8.987606091200235E-5	5.99173739413349E-5
7	2.995868697066745E-5	0.0	0.0	1.198347478826698E-4	5.99173739413349E-5
8	2.995868697066745E-5	2.995868697066745E-5	0.0	1.198347478826698E-4	8.987606091200235E-5
9	2.995868697066745E-5	2.995868697066745E-5	0.0	1.198347478826698E-4	8.987606091200235E-5
10-11	5.99173739413349E-5	2.995868697066745E-5	0.0	1.797521218240047E-4	1.198347478826698E-4
12-13	5.99173739413349E-5	2.995868697066745E-5	0.0	1.797521218240047E-4	2.546488392506733E-4
14-15	8.987606091200235E-5	2.995868697066745E-5	0.0	1.797521218240047E-4	2.9958686970667453E-4
16-17	8.987606091200235E-5	2.995868697066745E-5	0.0	1.9473146530933844E-4	3.595042436480094E-4
18-19	8.987606091200235E-5	2.995868697066745E-5	0.0	2.2469015228000587E-4	3.894629306186768E-4
20-21	8.987606091200235E-5	2.995868697066745E-5	0.0	2.396694957653396E-4	4.194216175893443E-4
22-23	8.987606091200235E-5	2.995868697066745E-5	0.0	2.396694957653396E-4	4.34400961074678E-4
24-25	8.987606091200235E-5	2.995868697066745E-5	0.0	2.546488392506733E-4	4.643596480453455E-4
26-27	8.987606091200235E-5	2.995868697066745E-5	0.0	2.846075262213408E-4	5.392563654720141E-4
28-29	1.198347478826698E-4	5.99173739413349E-5	0.0	4.4938030456001174E-4	5.692150524426816E-4
30-31	1.198347478826698E-4	5.99173739413349E-5	0.0	0.0011384301048853632	5.692150524426816E-4
32-33	1.198347478826698E-4	5.99173739413349E-5	0.0	0.003310434910258753	5.99173739413349E-4
34-35	1.4979343485333724E-4	1.4979343485333724E-4	0.0	0.006096592798530826	5.99173739413349E-4
36-37	1.4979343485333724E-4	1.797521218240047E-4	0.0	0.010440602409277607	5.99173739413349E-4
38-39	1.4979343485333724E-4	2.0971080879467213E-4	0.0	0.018589365265299154	6.291324263840164E-4
40-41	1.797521218240047E-4	2.846075262213408E-4	0.0	0.0280263516610594	6.590911133546839E-4
42-43	1.797521218240047E-4	3.2954555667734196E-4	0.0	0.0372236685610543	6.590911133546839E-4
44-45	1.797521218240047E-4	3.2954555667734196E-4	0.0	0.04763431228336125	6.590911133546839E-4
46-47	1.797521218240047E-4	3.2954555667734196E-4	0.0	0.05741582357928417	6.890498003253513E-4
48-49	1.797521218240047E-4	3.2954555667734196E-4	0.0	0.06845559972797513	7.190084872960188E-4
50-51	1.797521218240047E-4	3.595042436480094E-4	0.0	0.07894114016770873	7.190084872960188E-4
52-53	1.797521218240047E-4	3.894629306186768E-4	0.0	0.08957647404229568	7.939052047226874E-4
54-55	1.797521218240047E-4	4.1942161758934426E-4	0.0	0.10106563049554665	8.088845482080211E-4
56-57	1.797521218240047E-4	4.4938030456001174E-4	0.0	0.11379807245808031	8.238638916933549E-4
58-59	1.797521218240047E-4	5.092976785013466E-4	0.0	0.1264556177031873	8.538225786640223E-4
60-61	1.797521218240047E-4	5.092976785013466E-4	0.0	0.13996698552695833	8.68801922149356E-4
62-63	1.797521218240047E-4	5.242770219866803E-4	0.0	0.1533135805723907	9.28719296090691E-4
64-65	1.797521218240047E-4	5.99173739413349E-4	0.0	0.16658527890039634	9.28719296090691E-4
66-67	1.797521218240047E-4	5.99173739413349E-4	0.0	0.18053104768524206	9.28719296090691E-4
68-69	1.797521218240047E-4	6.291324263840164E-4	0.0	0.19537557707920777	9.436986395760247E-4
70-71	1.797521218240047E-4	6.441117698693502E-4	0.0	0.21069944546470418	9.88636670032026E-4
72-73	1.797521218240047E-4	6.590911133546839E-4	0.0	0.2263528594068779	0.0011833681353413642
74-75	1.797521218240047E-4	7.04029143810685E-4	0.0	0.24223096350133166	0.0012432855092826991
76-77	1.797521218240047E-4	7.190084872960188E-4	0.0	0.2591426422962735	0.0012582648527680329
78-79	1.797521218240047E-4	7.489671742666863E-4	0.0	0.2772077305395859	0.0012582648527680329
80-81	1.797521218240047E-4	7.489671742666863E-4	0.0	0.29416434736498365	0.0013331615701947016
82-83	1.797521218240047E-4	7.489671742666863E-4	0.0	0.3110161087859841	0.00152789303550404
84-85	2.6962818273600705E-4	7.489671742666863E-4	0.0	0.3301447304167553	0.00152789303550404
86-87	2.6962818273600705E-4	7.489671742666863E-4	0.0	0.34958791826071844	0.0015578517224747075
88-89	2.6962818273600705E-4	7.489671742666863E-4	0.0	0.3689711887307403	0.001632748439901376
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCTG	4465	0.0	35.200966	1
GTATCAA	7170	0.0	33.2798	1
TACCTGG	4690	0.0	32.60856	2
TCAACGC	8260	0.0	27.830458	4
CAACGCA	8555	0.0	26.915	5
ACCTGGG	5460	0.0	26.879482	3
TATCAAC	8905	0.0	26.02762	2
ATCAACG	8895	0.0	25.736895	3
AACGCAG	9205	0.0	25.325027	6
GGTATCA	4830	0.0	24.553553	1
GTACATG	9650	0.0	23.888023	1
TACATGG	10050	0.0	22.684212	2
ACATGGG	9975	0.0	22.045662	3
ACGCAGA	10665	0.0	21.591988	7
CGCAGAG	11280	0.0	20.330885	8
AGTACAT	7200	0.0	20.11944	2
GAGTACA	7675	0.0	19.733807	1
GTGTAGC	2030	0.0	19.708126	1
CATGGGG	7685	0.0	19.653427	4
GTATAAG	895	0.0	18.625465	1
>>END_MODULE