FastQCFastQC Report
Mon 15 Aug 2016
SRR2921464_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921464_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2416485
Sequences flagged as poor quality0
Sequence length98
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG28700.031.6021631
GTATCAA64350.028.1174871
TACCTGG30950.027.9988062
TCAACGC74800.023.7831024
TATCAAC75700.023.5609112
CAACGCA75550.023.4863135
GTACATG74750.023.1584321
ATCAACG76600.023.044663
AACGCAG80850.022.053276
TACATGG77450.022.0221582
ACCTGGG39550.021.5627823
ACATGGG79850.020.6137923
GGTATCA45700.020.4508971
GTGTAGC12450.019.9690271
CATGGGG58000.019.3676284
ACGCAGA92450.019.1810597
CGCAGAG96850.018.3043378
TACGGTC1550.001220712917.7483465
TATAACG1550.001220712917.7483462
GTATAGG5750.017.615191
Produced by FastQC (version 0.11.5)