##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921464_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2416485
Sequences flagged as poor quality	0
Sequence length	98
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.81401995046524	31.0	31.0	34.0	28.0	34.0
2	30.9399814192929	31.0	31.0	34.0	28.0	34.0
3	31.074815279217542	33.0	31.0	34.0	28.0	34.0
4	34.59226479783653	37.0	35.0	37.0	32.0	37.0
5	34.59870431639344	37.0	35.0	37.0	32.0	37.0
6	34.69240032526583	37.0	35.0	37.0	32.0	37.0
7	34.606814857116845	37.0	35.0	37.0	32.0	37.0
8	34.59671713252927	37.0	35.0	37.0	32.0	37.0
9	36.102691719584435	39.0	37.0	39.0	32.0	39.0
10-11	35.9953676517752	39.0	37.0	39.0	32.0	39.0
12-13	35.86415330531743	39.0	37.0	39.0	31.0	39.0
14-15	36.96020604307496	40.0	37.0	41.0	31.0	41.0
16-17	36.83784256885518	40.0	37.0	41.0	31.0	41.0
18-19	36.67407784447245	40.0	37.0	41.0	30.5	41.0
20-21	36.57180574263859	40.0	37.0	41.0	30.0	41.0
22-23	36.630974742239246	40.0	37.0	41.0	30.0	41.0
24-25	36.531101165535894	40.0	37.0	41.0	30.0	41.0
26-27	36.297637891400115	39.5	36.0	41.0	29.5	41.0
28-29	36.09986550713123	39.0	36.0	41.0	29.0	41.0
30-31	35.87949790708405	39.0	36.0	40.0	28.0	41.0
32-33	35.63923053526093	39.0	35.5	40.0	27.0	41.0
34-35	35.25105576901988	39.0	35.0	40.0	26.0	41.0
36-37	34.90274448217142	38.0	35.0	40.0	25.0	41.0
38-39	34.706589322921516	38.0	34.0	40.0	24.0	41.0
40-41	34.474621195662294	38.0	34.0	40.0	24.0	41.0
42-43	34.220798184139355	38.0	33.5	40.0	23.0	41.0
44-45	33.938504687593756	38.0	33.0	40.0	21.5	41.0
46-47	33.684859206657606	38.0	33.0	40.0	20.0	41.0
48-49	33.50468986979021	38.0	33.0	40.0	19.0	41.0
50-51	32.73741364005984	37.0	32.0	39.0	18.0	40.5
52-53	33.22156706952454	37.5	33.0	39.0	20.0	40.0
54-55	33.68729518287927	38.0	33.0	40.0	20.0	41.0
56-57	33.56975503675794	38.0	33.0	40.0	19.0	41.0
58-59	33.27305528484555	37.5	33.0	40.0	18.0	41.0
60-61	32.84807726925679	37.0	32.5	39.5	14.0	41.0
62-63	32.394479791929186	36.0	32.0	39.0	10.0	40.5
64-65	31.875504296529876	35.5	31.0	39.0	7.5	40.0
66-67	31.41168825794491	35.0	31.0	38.5	6.5	40.0
68-69	30.907986186547816	35.0	30.0	38.0	2.0	40.0
70-71	30.395985905147352	34.5	30.0	37.0	2.0	39.0
72-73	29.848519026602688	34.0	29.0	36.5	2.0	39.0
74-75	29.26258387699489	34.0	29.0	36.0	2.0	38.5
76-77	28.682798155171664	34.0	28.0	35.0	2.0	37.5
78-79	28.133078831443193	33.0	27.0	35.0	2.0	37.0
80-81	27.56400391477704	33.0	26.0	35.0	2.0	36.0
82-83	26.94856827168387	32.5	25.5	35.0	2.0	36.0
84-85	26.34816768984703	32.0	24.5	34.0	2.0	35.0
86-87	25.748818014595578	32.0	23.5	34.0	2.0	35.0
88-89	25.115791945739367	31.0	20.0	34.0	2.0	35.0
90-91	24.391262101771787	31.0	17.5	34.0	2.0	35.0
92-93	23.591098020471883	30.5	5.0	34.0	2.0	35.0
94-95	22.688905579798757	30.0	2.0	34.0	2.0	35.0
96-97	21.781106027970377	29.0	2.0	34.0	2.0	35.0
98	21.13624375901361	29.0	2.0	34.0	2.0	34.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	54809.0
3	9689.0
4	7497.0
5	6631.0
6	6008.0
7	6247.0
8	7035.0
9	8005.0
10	9461.0
11	10369.0
12	11980.0
13	14490.0
14	11830.0
15	11583.0
16	13117.0
17	15017.0
18	16955.0
19	16519.0
20	17245.0
21	18494.0
22	20390.0
23	22801.0
24	25788.0
25	29575.0
26	33900.0
27	38914.0
28	46028.0
29	54618.0
30	65707.0
31	81376.0
32	102746.0
33	136370.0
34	187840.0
35	272478.0
36	389852.0
37	435153.0
38	192435.0
39	7533.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.01687361030888	18.01912955175171	15.078629435523947	23.885367402415458
2	15.434162014006628	19.31266910530227	38.93987666051832	26.313292220172773
3	19.86629019896227	23.260462071458473	27.71393588869276	29.1593118408865
4	14.104210237348855	15.848914575032389	35.41169714181846	34.6351780458003
5	15.867579341416551	35.500107665805345	32.47979195653614	16.152521036241964
6	33.57371794871795	33.66328761677599	17.427656860796397	15.335337573709667
7	30.535651298390533	30.330555109354272	19.793065645684536	19.340727946570663
8	27.741970198675496	32.9523178807947	19.318915562913908	19.986796357615894
9	26.583533716026338	17.40804814151088	19.515936114824456	36.49248202763833
10-11	26.255747592059254	25.448047810529317	26.008719349883417	22.28748524752801
12-13	26.562020171920718	22.34021595626244	26.652305451053916	24.445458420762925
14-15	23.68934052002975	23.909506562351133	24.462022133733644	27.939130783885474
16-17	24.049732849726393	27.081085007363992	24.899556091210414	23.9696260516992
18-19	23.986897537325806	26.432222683897287	26.308129394064927	23.27275038471197
20-21	24.626991935649208	25.51978417750394	26.186908792546692	23.66631509430016
22-23	24.341059549152874	25.254506130723875	26.09670135199456	24.307732968128686
24-25	24.145767293218213	25.507826873075224	26.164340418817787	24.182065414888772
26-27	24.18628115093915	25.626705008939098	26.115129948784237	24.071883891337514
28-29	24.12933803658989	25.763832211122185	25.985941175605664	24.120888576682265
30-31	24.284141425183183	25.62594407123266	25.93516880656146	24.154745697022697
32-33	24.14701177803057	25.6387631826017	25.9725816787295	24.24164336063823
34-35	24.303593710331715	25.643719535276233	25.96135667561538	24.09133007877667
36-37	24.305963496565035	25.70879005662574	25.928750229320404	24.056496217488824
38-39	24.296745667918348	25.671535614666702	25.909405182472124	24.12231353494283
40-41	24.406108108771836	25.600728580921743	25.85361325814154	24.13955005216488
42-43	24.33278917531972	25.551984706427238	25.82581839048796	24.289407727765084
44-45	24.38847359873117	25.59734900343191	25.78347545749932	24.2307019403376
46-47	24.47258919909274	25.650969079644753	25.69735543681388	24.17908628444863
48-49	24.39401717010282	25.672373672287147	25.705007516807783	24.228601640802246
50-51	24.26687818351791	25.8120661959543	25.68725294777689	24.233802672750894
52-53	24.3933073731652	25.81346903017453	25.567843979167463	24.225379617492802
54-55	24.31392871897494	25.63198977685786	25.742795983587197	24.311285520580007
56-57	24.404477215427143	25.66435197272546	25.72206155952773	24.209109252319667
58-59	24.40463017810553	25.62207566822853	25.705687430115542	24.267606723550397
60-61	24.444966338525184	25.597573345071094	25.83123997873224	24.12622033767148
62-63	24.293109585515783	25.737982296039235	25.72004907295431	24.24885904549068
64-65	24.239362245582726	25.617048956278715	25.81726798615904	24.326320811979514
66-67	24.353031508018592	25.67713798019075	25.682112459447065	24.287718052343592
68-69	24.283521829031354	25.66200259177684	25.701717650274897	24.35275792891691
70-71	24.37578183221548	25.48315567443841	25.74583882279357	24.39522367055254
72-73	24.361571993573516	25.512214309236242	25.776616516593432	24.34959718059681
74-75	24.24564913697759	25.743241038299903	25.687473667429007	24.323636157293503
76-77	24.321359976334207	25.523899766314873	25.67738231081972	24.477357946531196
78-79	24.34837288100361	25.475704946317023	25.789363183769343	24.386558988910025
80-81	24.359729879563304	25.64612160741075	25.56831657812525	24.4258319349007
82-83	24.377150173178148	25.58676714790073	25.612069723669677	24.42401295525145
84-85	24.385214300529938	25.57024670405199	25.622163834501986	24.422375160916086
86-87	24.40765548608321	25.530875241382507	25.714378946270138	24.347090326264144
88-89	24.278493870963516	25.466338376535585	25.630019129005504	24.62514862349539
90-91	24.359332793576126	25.46629929918174	25.599956763992722	24.574411143249414
92-93	24.365572655236225	25.613278207700528	25.657987587379193	24.36316154968405
94-95	24.429266601268345	25.468571907284055	25.5915939250655	24.5105675663821
96-97	24.38325605217772	25.4931363649497	25.728523948982172	24.39508363389041
98	24.354186785215585	25.592789622923483	25.474728469740388	24.578295122120544
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	189.0
1	135.0
2	101.5
3	127.0
4	132.5
5	138.5
6	191.0
7	294.0
8	399.0
9	467.0
10	525.0
11	648.5
12	822.0
13	1000.0
14	1183.0
15	1397.0
16	1580.0
17	1660.5
18	1632.5
19	1574.5
20	1486.5
21	1401.5
22	1432.5
23	1513.5
24	1689.5
25	2017.0
26	2469.0
27	3152.0
28	4126.0
29	5393.0
30	7035.5
31	9269.5
32	12472.0
33	16481.0
34	21557.0
35	28330.0
36	36462.0
37	45230.5
38	54053.5
39	62835.0
40	71308.0
41	79108.5
42	86855.0
43	94966.5
44	101067.0
45	106179.0
46	113161.5
47	119678.0
48	121603.0
49	118715.0
50	116546.0
51	114332.0
52	111295.0
53	108835.0
54	104231.0
55	97510.0
56	89026.5
57	80305.5
58	71360.5
59	62193.0
60	53282.0
61	43960.5
62	34903.5
63	26176.5
64	18952.5
65	14038.5
66	10468.0
67	7797.0
68	5907.0
69	4489.5
70	3401.5
71	2564.0
72	1919.5
73	1373.5
74	993.5
75	728.5
76	470.5
77	288.5
78	179.5
79	113.0
80	68.0
81	40.0
82	23.0
83	17.0
84	13.5
85	10.5
86	11.0
87	9.0
88	7.0
89	6.0
90	5.0
91	5.0
92	4.5
93	4.5
94	3.0
95	2.0
96	2.0
97	3.5
98	3.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.4851675056952557
2	0.04965890539357787
3	0.058100919310486096
4	0.05578350372545247
5	0.06641878596391039
6	0.06641878596391039
7	0.04274804105963828
8	0.02007047426323772
9	0.011380165819361594
10-11	0.015083892513299276
12-13	0.010552517396135296
14-15	0.01464937709110547
16-17	0.013883802299621145
18-19	0.05654907851693679
20-21	0.08663409870121272
22-23	0.10329052321864196
24-25	0.07281237003333355
26-27	0.08655133385889008
28-29	0.08812386586302005
30-31	0.05847336110093793
32-33	0.03314731935021322
34-35	0.4058787867501764
36-37	0.6359857396176678
38-39	0.5842370219554436
40-41	0.5796228819959569
42-43	0.5538623248230384
44-45	0.5384473729404486
46-47	0.5280810764395393
48-49	0.5188941789417274
50-51	0.5335849384539941
52-53	0.6148186311936552
54-55	0.58318177021583
56-57	0.5409303182101275
58-59	0.5179837656761784
60-61	0.5316399646594123
62-63	0.5431235865316771
64-65	0.5634837377430442
66-67	0.5886028673879623
68-69	0.5427097623200641
70-71	0.49149901613293695
72-73	0.4732907508219583
74-75	0.45340649745394657
76-77	0.4686766108624717
78-79	0.4618278201602741
80-81	0.45990353757627295
82-83	0.47968433489138146
84-85	0.44395061421858606
86-87	0.4474060463855559
88-89	0.4758357697233792
90-91	0.4586206825202722
92-93	0.4531168205058173
94-95	0.46449698632517894
96-97	0.45895174188956267
98	0.45322027655872066
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	2416485.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.06800101754882
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.00985530717381	51.41013345523571
2	12.590758309668102	16.385109490103904
3	3.883142501025965	7.580049606241338
4	1.600034891635198	4.164442878281307
5	0.8390859843740776	2.7298823842531714
6	0.47959134310153684	1.8723630000563045
7	0.3243316670503771	1.4772529269160066
8	0.2515254094920121	1.30930044806125
9	0.16385573664923744	0.9595588715121414
>10	0.8107499412275448	9.026709055651349
>50	0.035817822429890005	1.5579274005694765
>100	0.010677357685354258	1.1653636316556457
>500	3.824856579076658E-4	0.1771580702115908
>1k	1.9124282895381596E-4	0.18474878125084576
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	4.1382421161314887E-5	0.0	0.0	0.0
3	0.0	4.1382421161314887E-5	0.0	0.0	0.0
4	0.0	4.1382421161314887E-5	0.0	4.1382421161314887E-5	0.0
5	0.0	4.1382421161314887E-5	0.0	4.1382421161314887E-5	0.0
6	0.0	4.1382421161314887E-5	0.0	4.1382421161314887E-5	0.0
7	0.0	4.1382421161314887E-5	0.0	4.1382421161314887E-5	0.0
8	0.0	4.1382421161314887E-5	0.0	4.1382421161314887E-5	0.0
9	0.0	4.1382421161314887E-5	0.0	4.1382421161314887E-5	0.0
10-11	0.0	4.1382421161314887E-5	0.0	4.1382421161314887E-5	0.0
12-13	0.0	4.1382421161314887E-5	0.0	4.1382421161314887E-5	2.0691210580657443E-5
14-15	4.1382421161314887E-5	6.207363174197233E-5	0.0	8.276484232262977E-5	4.1382421161314887E-5
16-17	1.0345605290328723E-4	8.276484232262977E-5	0.0	8.276484232262977E-5	4.1382421161314887E-5
18-19	1.2414726348394467E-4	8.276484232262977E-5	0.0	8.276484232262977E-5	4.1382421161314887E-5
20-21	1.2414726348394467E-4	1.2414726348394467E-4	0.0	1.6552968464525955E-4	4.1382421161314887E-5
22-23	1.2414726348394467E-4	1.2414726348394467E-4	0.0	1.6552968464525955E-4	4.1382421161314887E-5
24-25	1.2414726348394467E-4	1.2414726348394467E-4	0.0	2.0691210580657443E-4	4.1382421161314887E-5
26-27	1.2414726348394467E-4	1.2414726348394467E-4	0.0	2.0691210580657443E-4	4.1382421161314887E-5
28-29	1.2414726348394467E-4	1.2414726348394467E-4	0.0	3.7244179045183397E-4	4.1382421161314887E-5
30-31	1.2414726348394467E-4	1.2414726348394467E-4	0.0	6.621187385810382E-4	4.1382421161314887E-5
32-33	1.2414726348394467E-4	1.2414726348394467E-4	0.0	0.0014897671618073359	4.1382421161314887E-5
34-35	1.6552968464525955E-4	1.2414726348394467E-4	0.0	0.0025450189014208655	4.1382421161314887E-5
36-37	1.6552968464525955E-4	1.448384740646021E-4	0.0	0.004200315747873461	4.1382421161314887E-5
38-39	1.6552968464525955E-4	1.6552968464525955E-4	0.0	0.0071384676503268175	4.1382421161314887E-5
40-41	1.6552968464525955E-4	1.6552968464525955E-4	0.0	0.011628460346329483	4.1382421161314887E-5
42-43	2.0691210580657443E-4	1.6552968464525955E-4	0.0	0.015870158515364257	4.1382421161314887E-5
44-45	2.896769481292042E-4	1.6552968464525955E-4	0.0	0.020277386369044297	4.1382421161314887E-5
46-47	2.896769481292042E-4	1.6552968464525955E-4	0.0	0.024705305433304986	4.1382421161314887E-5
48-49	2.896769481292042E-4	2.0691210580657445E-4	0.0	0.03033331471124381	6.207363174197233E-5
50-51	2.896769481292042E-4	2.4829452696788933E-4	0.0	0.03536127888234357	8.276484232262977E-5
52-53	3.7244179045183397E-4	2.4829452696788933E-4	0.0	0.040161639737056096	8.276484232262977E-5
54-55	3.7244179045183397E-4	2.896769481292042E-4	0.0	0.045706884172672296	8.276484232262977E-5
56-57	3.931330010324914E-4	2.896769481292042E-4	0.0	0.05193493855745018	8.276484232262977E-5
58-59	4.1382421161314885E-4	2.896769481292042E-4	0.0	0.05890787652313174	8.276484232262977E-5
60-61	4.1382421161314885E-4	2.896769481292042E-4	0.0	0.06561182875126476	8.276484232262977E-5
62-63	4.1382421161314885E-4	3.1036815870986165E-4	0.0	0.07299859092855945	1.2414726348394467E-4
64-65	4.1382421161314885E-4	3.310593692905191E-4	0.0	0.0810474718444352	1.2414726348394467E-4
66-67	4.1382421161314885E-4	4.1382421161314885E-4	0.0	0.08990330997295659	1.448384740646021E-4
68-69	4.1382421161314885E-4	4.1382421161314885E-4	0.0	0.0988419129438006	1.6552968464525955E-4
70-71	4.1382421161314885E-4	4.345154221938063E-4	0.0	0.10873231160135485	1.6552968464525955E-4
72-73	4.1382421161314885E-4	4.5520663277446373E-4	0.0	0.11862271025890911	2.0691210580657443E-4
74-75	4.1382421161314885E-4	4.5520663277446373E-4	0.0	0.12774753412497905	2.0691210580657443E-4
76-77	4.1382421161314885E-4	4.5520663277446373E-4	0.0	0.13794830094124316	2.0691210580657443E-4
78-79	4.1382421161314885E-4	4.5520663277446373E-4	0.0	0.14912155465479818	2.0691210580657443E-4
80-81	4.1382421161314885E-4	4.5520663277446373E-4	0.0	0.16139144252912804	2.0691210580657443E-4
82-83	4.1382421161314885E-4	4.5520663277446373E-4	0.0	0.17455105245842617	3.310593692905191E-4
84-85	4.1382421161314885E-4	4.5520663277446373E-4	0.0	0.18684163154333672	3.310593692905191E-4
86	4.1382421161314885E-4	4.5520663277446373E-4	0.0	0.19582161693534203	3.7244179045183397E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCTG	2870	0.0	31.602163	1
GTATCAA	6435	0.0	28.117487	1
TACCTGG	3095	0.0	27.998806	2
TCAACGC	7480	0.0	23.783102	4
TATCAAC	7570	0.0	23.560911	2
CAACGCA	7555	0.0	23.486313	5
GTACATG	7475	0.0	23.158432	1
ATCAACG	7660	0.0	23.04466	3
AACGCAG	8085	0.0	22.05327	6
TACATGG	7745	0.0	22.022158	2
ACCTGGG	3955	0.0	21.562782	3
ACATGGG	7985	0.0	20.613792	3
GGTATCA	4570	0.0	20.450897	1
GTGTAGC	1245	0.0	19.969027	1
CATGGGG	5800	0.0	19.367628	4
ACGCAGA	9245	0.0	19.181059	7
CGCAGAG	9685	0.0	18.304337	8
TACGGTC	155	0.0012207129	17.748346	5
TATAACG	155	0.0012207129	17.748346	2
GTATAGG	575	0.0	17.61519	1
>>END_MODULE