FastQCFastQC Report
Mon 15 Aug 2016
SRR2921464_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921464_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2416485
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG28300.033.834531
GTATCAA69650.028.9314211
TACCTGG32100.028.5641632
TCAACGC78300.025.3023914
CAACGCA79850.024.8532145
ATCAACG81600.024.3368533
TATCAAC81900.024.3052082
AACGCAG83450.023.7830266
GTACATG78600.023.758231
ACCTGGG39900.022.5043853
TACATGG80450.022.203992
ACGCAGA97600.020.2875887
ACATGGG84000.020.2479923
TCGCGAA600.004593728619.91777482-83
CGCAGAG101150.019.5755678
GTATAGG6350.019.5051881
GGTATCA49200.019.2680841
CGTATAC1759.9276745E-519.0037023
AGAGTAC77050.017.77793710-11
GTACACG5650.017.7060381
Produced by FastQC (version 0.11.5)