##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921464_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2416485
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.01708680169751	33.0	31.0	34.0	30.0	34.0
2	32.14461335369349	34.0	31.0	34.0	30.0	34.0
3	32.267844410372916	34.0	31.0	34.0	30.0	34.0
4	35.79244522519279	37.0	35.0	37.0	35.0	37.0
5	35.604424608470566	37.0	35.0	37.0	35.0	37.0
6	35.64042524575985	37.0	35.0	37.0	35.0	37.0
7	35.58441620783907	37.0	35.0	37.0	35.0	37.0
8	35.54231869844009	37.0	35.0	37.0	33.0	37.0
9	37.180285000734536	39.0	37.0	39.0	34.0	39.0
10-11	37.09484540562015	39.0	37.0	39.0	34.0	39.0
12-13	36.98907379934078	39.0	37.0	39.0	33.0	39.0
14-15	38.317471037477986	40.0	38.0	41.0	34.0	41.0
16-17	38.23405649114313	40.0	38.0	41.0	33.5	41.0
18-19	38.136695034316375	40.0	38.0	41.0	33.5	41.0
20-21	38.03126855742949	40.0	38.0	41.0	33.0	41.0
22-23	38.004524133193456	40.0	38.0	41.0	33.5	41.0
24-25	38.070854153863976	40.0	38.0	41.0	33.5	41.0
26-27	37.977422371750706	40.0	38.0	41.0	33.0	41.0
28-29	37.85759439847547	40.0	38.0	41.0	33.0	41.0
30-31	37.73192550336543	40.0	38.0	41.0	33.0	41.0
32-33	37.58633862821412	40.0	38.0	41.0	32.5	41.0
34-35	37.45898298561754	40.0	37.5	41.0	32.0	41.0
36-37	37.37907373726715	40.0	37.0	41.0	32.0	41.0
38-39	37.362296062255716	40.0	37.0	41.0	32.0	41.0
40-41	37.21915116377714	40.0	37.0	41.0	31.5	41.0
42-43	37.009089234983875	40.0	37.0	41.0	31.0	41.0
44-45	36.8263835695235	40.0	36.5	41.0	31.0	41.0
46-47	36.58396203576683	39.0	36.0	41.0	30.0	41.0
48-49	36.282011889169596	39.0	36.0	40.0	30.0	41.0
50-51	36.03327891544951	39.0	35.0	40.0	29.5	41.0
52-53	35.75304895333511	39.0	35.0	40.0	28.5	41.0
54-55	35.45389998282629	38.0	35.0	40.0	28.0	41.0
56-57	35.10844304847744	38.0	34.0	40.0	27.5	41.0
58-59	34.75202080708136	38.0	34.0	40.0	26.5	41.0
60-61	34.354021233320296	37.0	34.0	40.0	26.0	41.0
62-63	33.940578981454465	36.5	33.0	39.0	26.0	40.5
64-65	33.4572248534545	36.0	33.0	39.0	25.0	40.0
66-67	32.94735969807385	35.5	32.0	38.5	23.0	40.0
68-69	32.46034157050427	35.0	31.5	38.0	22.0	40.0
70-71	31.916063414422187	35.0	31.0	37.0	21.5	39.0
72-73	31.371656972834508	34.5	30.5	36.5	20.0	39.0
74-75	30.851607603606062	34.0	30.0	36.0	18.0	38.5
76-77	29.50914861875824	32.5	28.5	34.5	18.0	36.5
78-79	30.43997500501762	34.0	30.0	35.0	18.0	37.0
80-81	30.47580762967699	34.0	30.0	35.0	18.0	37.0
82-83	30.14251712714956	34.0	30.0	35.0	16.5	36.0
84-85	29.667002898838604	34.0	30.0	35.0	9.0	36.0
86-87	29.16957998911642	34.0	29.0	35.0	6.0	35.5
88-89	28.644684531457884	33.5	29.0	35.0	2.0	35.0
90-91	28.196239372476967	33.0	28.0	35.0	2.0	35.0
92-93	27.791852008185444	33.0	27.0	35.0	2.0	35.0
94-95	27.33396938114658	33.0	26.5	34.0	2.0	35.0
96-97	26.603166789779372	32.0	25.0	34.0	2.0	35.0
98-99	25.62472516899546	32.0	24.0	34.0	2.0	35.0
100-101	21.936700414031122	28.0	2.0	32.5	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	14134.0
3	7252.0
4	4127.0
5	3058.0
6	2794.0
7	2809.0
8	3269.0
9	3818.0
10	4565.0
11	5498.0
12	6526.0
13	7764.0
14	8614.0
15	9609.0
16	11235.0
17	13152.0
18	13440.0
19	11728.0
20	11514.0
21	12262.0
22	13210.0
23	14844.0
24	17156.0
25	20011.0
26	24300.0
27	29298.0
28	35846.0
29	44080.0
30	54934.0
31	68684.0
32	89422.0
33	119941.0
34	174206.0
35	268360.0
36	436529.0
37	577196.0
38	263640.0
39	7660.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.43115295371919	18.496630352607973	15.42631343180102	23.64590326187182
2	15.507234681779527	19.60885335518325	38.70729592776285	26.176616035274375
3	19.884172360863435	23.468008967247695	27.897667100240902	28.75015157164797
4	14.31784651147961	16.09087457210517	35.459360394795745	34.13191852161948
5	15.899854126361745	35.489462957406964	32.55945126682461	16.051231649406677
6	33.64818106077044	33.70694067390941	17.383901108275044	15.260977157045108
7	30.669297240796094	30.340806781182785	19.763513373603647	19.226382604417477
8	27.820078062667204	33.0419584764938	19.304478371206127	19.833485089632877
9	26.628960306842238	17.406338147763094	19.492351725366262	36.47234982002841
10-11	26.289357258412437	25.41630602793275	26.12753842550048	22.166798288154336
12-13	26.65656512100315	22.33098827248657	26.742205958862574	24.270240647647704
14-15	23.691375411452384	23.917428354799185	24.596498187725395	27.794698046023036
16-17	24.005362578442302	27.057321302236392	25.06610079610735	23.871215323213953
18-19	23.98279154662879	26.44369393891347	26.46898831201251	23.104526202445232
20-21	24.625672206721458	25.509012325161056	26.35315267358368	23.512162794533804
22-23	24.283529293168073	25.18427317534419	26.34647393390239	24.185723597585348
24-25	24.115672701411363	25.510643388729786	26.327432712472444	24.046251197386407
26-27	24.120747363447602	25.547783483061682	26.377651530751518	23.953817622739198
28-29	24.0870212728969	25.797495631096496	26.200030795492268	23.915452300514335
30-31	24.293052796068217	25.6393262569985	26.100071552397335	23.967549394535947
32-33	24.132590287626723	25.568261530458404	26.14520346918362	24.153944712731253
34-35	24.29522913032925	25.624844933337116	26.142309759199968	23.937616177133673
36-37	24.26073424757317	25.713440236071705	26.101423093557486	23.924402422797638
38-39	24.249313042121415	25.678773156761324	26.10876595089498	23.96314785022228
40-41	24.34169121672032	25.55893927211807	26.08760618092225	24.011763330239354
42-43	24.326482995479022	25.48800354576837	26.056058978609308	24.129454480143302
44-45	24.28884945496686	25.532570062755124	26.01904899866005	24.159531483617965
46-47	24.38615764217644	25.609247775870497	25.98000156464142	24.02459301731164
48-49	24.357133590200558	25.6786527692683	25.882900907063682	24.08131273346746
50-51	24.3166870558321	25.765338639482582	25.839335583590888	24.078638721094425
52-53	24.37469182307691	25.844505641280097	25.706947257077957	24.07385527856504
54-55	24.356003734487228	25.637602445956702	25.817808835968936	24.188584983587127
56-57	24.403821287815127	25.72483028028557	25.764496553876093	24.106851878023207
58-59	24.464698101171404	25.61437406358507	25.76124158299929	24.15968625224424
60-61	24.485609736313688	25.64332407972997	25.8869140143257	23.984152169630633
62-63	24.270876855776272	25.709867211668534	25.883077480864614	24.13617845169058
64-65	24.28531018674959	25.639730489934966	25.90548689831046	24.169472425004983
66-67	24.350921801782707	25.673151480351606	25.82427704601164	24.15164967185405
68-69	24.351628212045412	25.644500169978524	25.78284322514473	24.221028392831336
70-71	24.395181149733176	25.522788470054774	25.83627944629907	24.245750933912984
72-73	24.429269156949147	25.485693256582522	25.820213756156868	24.264823830311467
74-75	24.26567308659723	25.780347203734287	25.68205465380445	24.271925055864035
76-77	24.38194938535325	25.55080351976276	25.75192214612877	24.315324948755222
78-79	24.30546956564593	25.572071600110824	25.859437799236257	24.263021035006993
80-81	24.327370874333003	25.7609897047196	25.685586193478414	24.226053227468984
82-83	24.441588250695162	25.62072023685711	25.69860325916543	24.239088253282297
84-85	24.35493824733269	25.6252977534438	25.7350241658868	24.28473983333671
86-87	24.398941929811805	25.627315098215785	25.76130362467137	24.212439347301036
88-89	24.301693409084102	25.566778661980504	25.709976236358372	24.421551692577022
90-91	24.321907495612262	25.634075255510343	25.69370417632425	24.35031307255315
92-93	24.409161123271826	25.748560138812927	25.74810471582226	24.094174022092986
94-95	24.372002378852546	25.64101343753712	25.70036059398475	24.286623589625584
96-97	24.352120929701293	25.610156823567486	25.80288584499074	24.234836401740477
98-99	24.353290578057074	25.657925232345242	25.668768561959283	24.320015627638405
100-101	24.753339436388888	25.111017946625303	25.848061231415613	24.287581385570192
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	11.0
1	11.5
2	29.0
3	45.5
4	38.0
5	37.0
6	68.5
7	148.0
8	244.0
9	274.5
10	252.0
11	250.5
12	264.5
13	295.5
14	349.0
15	409.0
16	466.5
17	549.0
18	646.5
19	715.5
20	782.5
21	849.5
22	964.0
23	1192.5
24	1402.5
25	1621.5
26	2043.0
27	2646.0
28	3483.0
29	4590.5
30	6009.5
31	7784.0
32	10371.5
33	13902.5
34	18222.0
35	24141.0
36	31717.0
37	39700.5
38	48173.5
39	56400.0
40	65054.5
41	73019.5
42	80123.0
43	87757.5
44	95070.5
45	101488.0
46	106335.5
47	112464.5
48	119090.0
49	120679.5
50	116289.5
51	111899.5
52	109375.5
53	106144.5
54	101960.0
55	96406.0
56	88238.5
57	78447.5
58	69904.0
59	61898.0
60	52856.0
61	43970.0
62	35354.5
63	26580.0
64	19162.0
65	14084.5
66	10582.5
67	8105.0
68	6144.0
69	4605.0
70	3566.5
71	2630.5
72	1865.5
73	1361.0
74	1017.5
75	720.5
76	492.5
77	289.5
78	158.0
79	96.5
80	57.0
81	26.0
82	9.0
83	3.5
84	3.5
85	1.5
86	0.5
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.32178970695038456
2	0.0
3	0.005628009277938824
4	0.060583864580164994
5	4.1382421161314885E-4
6	0.06459795943281253
7	0.07461250535385074
8	0.10457337827464272
9	0.09166206287231247
10-11	0.09789011725709036
12-13	0.09654518856934763
14-15	0.06472210669629648
16-17	0.03542335251408554
18-19	0.03840288683770022
20-21	0.02708479465008059
22-23	0.04061684636983056
24-25	0.03339561387718111
26-27	0.02062913694891547
28-29	0.022636184375239243
30-31	0.0030622991659373018
32-33	0.0051314202240030455
34-35	0.007676439125423912
36-37	0.011173253713555018
38-39	0.016470203622203323
40-41	0.02079466663356073
42-43	0.0034554321669697926
44-45	0.014380391353556923
46-47	0.024663923012143672
48-49	0.0025450189014208655
50-51	0.006993629176262216
52-53	0.002338106795614291
54-55	0.0036830354833570244
56-57	0.003082990376517959
58-59	8.8972205496827E-4
60-61	0.00533833232980962
62-63	0.014339008932395607
64-65	0.0072212324926494475
66-67	0.03906500557628125
68-69	0.060997688791778144
70-71	0.01270440329652367
72-73	0.03263003908569678
74-75	0.051562496766998346
76-77	0.30867147944224776
78-79	0.07432282840572153
80-81	0.033726673246471627
82-83	0.02824350244259741
84-85	0.019698032472785888
86-87	0.11775367941452151
88-89	0.5302329623399277
90-91	0.13352038187698248
92-93	0.04750701949318949
94-95	0.008028189705295088
96-97	0.005752156541422769
98-99	0.010469752553812666
100-101	0.00864892602271481
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2416485.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.66983413920671
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.02492463899843	48.27139251710928
2	13.760536140124913	17.24741035136376
3	4.169068892268244	7.8382456798013145
4	1.7832632930158465	4.470272591993547
5	0.9359451906563818	2.9327764930911826
6	0.5488159895431796	2.06365242225694
7	0.35995932414531473	1.5791013798733475
8	0.2663379724667975	1.3353085247573444
9	0.19223413531427702	1.0842553238435604
>10	0.9065867494376729	9.80423367346336
>50	0.0397974229003986	1.7008080932394638
>100	0.01167072693874275	1.1978156464934842
>500	6.61172453725978E-4	0.2755879628493852
>1k	1.9835173611406425E-4	0.19913933986394355
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	4.1382421161314887E-5	0.0
5	0.0	0.0	0.0	4.1382421161314887E-5	0.0
6	0.0	0.0	0.0	4.1382421161314887E-5	0.0
7	0.0	0.0	0.0	4.1382421161314887E-5	0.0
8	0.0	0.0	0.0	4.1382421161314887E-5	0.0
9	0.0	0.0	0.0	1.2414726348394467E-4	0.0
10-11	0.0	0.0	0.0	1.2414726348394467E-4	0.0
12-13	0.0	0.0	0.0	1.2414726348394467E-4	2.0691210580657443E-5
14-15	0.0	0.0	0.0	2.0691210580657443E-4	6.207363174197233E-5
16-17	8.276484232262977E-5	0.0	0.0	2.4829452696788933E-4	8.276484232262977E-5
18-19	8.276484232262977E-5	0.0	0.0	2.896769481292042E-4	8.276484232262977E-5
20-21	8.276484232262977E-5	0.0	0.0	3.310593692905191E-4	1.2414726348394467E-4
22-23	8.276484232262977E-5	0.0	0.0	3.5175057987117653E-4	1.448384740646021E-4
24-25	8.276484232262977E-5	0.0	0.0	4.345154221938063E-4	1.6552968464525955E-4
26-27	8.276484232262977E-5	0.0	0.0	4.5520663277446373E-4	1.6552968464525955E-4
28-29	8.276484232262977E-5	4.1382421161314887E-5	0.0	5.58662685677751E-4	1.6552968464525955E-4
30-31	1.0345605290328723E-4	4.1382421161314887E-5	0.0	8.069572126456403E-4	1.6552968464525955E-4
32-33	1.2414726348394467E-4	4.1382421161314887E-5	0.0	0.0015518407935493082	1.6552968464525955E-4
34-35	1.2414726348394467E-4	4.1382421161314887E-5	0.0	0.0025450189014208655	1.6552968464525955E-4
36-37	1.448384740646021E-4	8.276484232262977E-5	0.0	0.004138242116131488	1.6552968464525955E-4
38-39	1.6552968464525955E-4	1.2414726348394467E-4	0.0	0.007014320386842873	1.6552968464525955E-4
40-41	1.6552968464525955E-4	1.2414726348394467E-4	0.0	0.011442239451103566	1.6552968464525955E-4
42-43	1.6552968464525955E-4	1.2414726348394467E-4	0.0	0.015663246409557685	1.6552968464525955E-4
44-45	1.8622089522591699E-4	1.2414726348394467E-4	0.0	0.020111856684399035	1.6552968464525955E-4
46-47	2.0691210580657443E-4	1.2414726348394467E-4	0.0	0.02458115816982104	1.6552968464525955E-4
48-49	2.0691210580657443E-4	1.2414726348394467E-4	0.0	0.030354005921824467	1.6552968464525955E-4
50-51	2.0691210580657443E-4	1.6552968464525955E-4	0.0	0.03525782282944028	3.310593692905191E-4
52-53	2.0691210580657443E-4	1.6552968464525955E-4	0.0	0.03995472763124952	3.7244179045183397E-4
54-55	2.276033163872319E-4	1.6552968464525955E-4	0.0	0.045251677539897825	4.5520663277446373E-4
56-57	2.4829452696788933E-4	2.0691210580657443E-4	0.0	0.051148672555385195	4.5520663277446373E-4
58-59	2.4829452696788933E-4	3.310593692905191E-4	0.0	0.05793538962584084	4.5520663277446373E-4
60-61	2.4829452696788933E-4	3.310593692905191E-4	0.0	0.06463934185397385	4.5520663277446373E-4
62-63	2.4829452696788933E-4	3.310593692905191E-4	0.0	0.07194333918894592	5.379714750970935E-4
64-65	2.896769481292042E-4	3.5175057987117653E-4	0.0	0.07997152889424101	5.379714750970935E-4
66-67	2.896769481292042E-4	4.1382421161314885E-4	0.0	0.0888273670227624	5.379714750970935E-4
68-69	2.896769481292042E-4	4.1382421161314885E-4	0.0	0.09755905788779984	5.379714750970935E-4
70-71	2.896769481292042E-4	4.1382421161314885E-4	0.0	0.10724254443954753	5.58662685677751E-4
72-73	2.896769481292042E-4	4.1382421161314885E-4	0.0	0.1167811925172306	6.207363174197233E-4
74-75	3.310593692905191E-4	4.1382421161314885E-4	0.0	0.1256784130669133	6.207363174197233E-4
76-77	4.1382421161314885E-4	4.1382421161314885E-4	0.0	0.13561019414562886	6.207363174197233E-4
78-79	4.1382421161314885E-4	4.1382421161314885E-4	0.0	0.1466386093851193	6.207363174197233E-4
80-81	4.1382421161314885E-4	4.1382421161314885E-4	0.0	0.15872227636422326	6.207363174197233E-4
82-83	4.345154221938063E-4	4.1382421161314885E-4	0.0	0.17163359176655346	6.621187385810382E-4
84-85	4.5520663277446373E-4	4.5520663277446373E-4	0.0	0.18357242027159282	6.621187385810382E-4
86-87	4.5520663277446373E-4	4.5520663277446373E-4	0.0	0.19561470482953547	6.621187385810382E-4
88-89	4.5520663277446373E-4	4.5520663277446373E-4	0.0	0.20887777081173686	7.241923703230106E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCTG	2830	0.0	33.83453	1
GTATCAA	6965	0.0	28.931421	1
TACCTGG	3210	0.0	28.564163	2
TCAACGC	7830	0.0	25.302391	4
CAACGCA	7985	0.0	24.853214	5
ATCAACG	8160	0.0	24.336853	3
TATCAAC	8190	0.0	24.305208	2
AACGCAG	8345	0.0	23.783026	6
GTACATG	7860	0.0	23.75823	1
ACCTGGG	3990	0.0	22.504385	3
TACATGG	8045	0.0	22.20399	2
ACGCAGA	9760	0.0	20.287588	7
ACATGGG	8400	0.0	20.247992	3
TCGCGAA	60	0.0045937286	19.917774	82-83
CGCAGAG	10115	0.0	19.575567	8
GTATAGG	635	0.0	19.505188	1
GGTATCA	4920	0.0	19.268084	1
CGTATAC	175	9.9276745E-5	19.003702	3
AGAGTAC	7705	0.0	17.777937	10-11
GTACACG	565	0.0	17.706038	1
>>END_MODULE