FastQCFastQC Report
Mon 15 Aug 2016
SRR2921463_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921463_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4681743
Sequences flagged as poor quality0
Sequence length98
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49120.10491818965714264No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA107150.033.8088951
GTACCTG64250.033.3141141
TACCTGG67050.031.387052
TCAACGC131350.027.0525514
TATCAAC133350.026.9218792
CAACGCA134650.026.3554955
ATCAACG136550.026.089513
GTACATG145250.025.2892061
AACGCAG142900.025.148866
ACCTGGG83750.024.964143
GGTATCA68800.023.8182581
TACATGG152700.022.9999182
ACATGGG154800.022.2140063
ACGCAGA162850.021.8655787
CGCAGAG172600.020.5726958
TATAACG3206.384653E-1018.6264462
CATGGGG120050.018.3704384
AGAGTAC144050.018.06662210-11
GCAGAGT193050.017.8714629
CCTGGGG86850.017.6852654
Produced by FastQC (version 0.11.5)