##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921463_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4681743
Sequences flagged as poor quality	0
Sequence length	98
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.429029103049867	31.0	31.0	34.0	27.0	34.0
2	30.539389496604148	31.0	31.0	34.0	27.0	34.0
3	30.66933362211467	31.0	31.0	34.0	27.0	34.0
4	34.18461308106831	37.0	35.0	37.0	30.0	37.0
5	34.174729155359444	37.0	35.0	37.0	30.0	37.0
6	34.26694182059972	37.0	35.0	37.0	30.0	37.0
7	34.15999959844015	37.0	35.0	37.0	30.0	37.0
8	34.14448294150277	37.0	35.0	37.0	30.0	37.0
9	35.60716916755149	39.0	37.0	39.0	30.0	39.0
10-11	35.476808316902485	39.0	35.0	39.0	30.0	39.0
12-13	35.32401490214222	39.0	35.0	39.0	30.0	39.0
14-15	36.338220829293704	40.0	36.0	41.0	29.5	41.0
16-17	36.189236252395744	40.0	36.0	41.0	29.0	41.0
18-19	36.01076468742518	39.0	36.0	41.0	28.0	41.0
20-21	35.895687033653914	39.0	36.0	41.0	27.0	41.0
22-23	35.94038149894174	39.0	36.0	41.0	27.5	41.0
24-25	35.80685430618468	39.0	36.0	41.0	27.0	41.0
26-27	35.53785513215911	39.0	36.0	40.5	27.0	41.0
28-29	35.30662906955807	39.0	35.0	40.0	25.5	41.0
30-31	35.053657046104405	39.0	35.0	40.0	25.0	41.0
32-33	34.785209162484996	38.0	34.5	40.0	24.0	41.0
34-35	34.36016158939096	38.0	34.0	40.0	22.5	41.0
36-37	33.975174096485006	38.0	33.5	40.0	19.0	41.0
38-39	33.74843578556106	38.0	33.0	40.0	18.0	41.0
40-41	33.485971464046614	38.0	33.0	40.0	16.5	41.0
42-43	33.19899426346127	38.0	32.5	40.0	15.0	41.0
44-45	32.883371321321995	38.0	32.0	40.0	13.0	41.0
46-47	32.59053903642297	37.0	31.0	40.0	9.0	41.0
48-49	32.37764097687549	37.0	31.0	40.0	8.5	41.0
50-51	31.64018731912452	36.0	30.5	39.0	8.0	40.0
52-53	32.169170007836826	37.0	31.5	39.0	8.0	40.0
54-55	32.605896991782764	37.0	32.0	40.0	7.0	40.5
56-57	32.45750685161488	37.0	32.0	40.0	4.0	41.0
58-59	32.123279940825455	36.5	31.5	40.0	2.0	41.0
60-61	31.67265834540683	36.0	31.0	39.0	2.0	40.5
62-63	31.18082336001784	35.5	30.5	39.0	2.0	40.0
64-65	30.637537985318716	35.0	29.5	38.5	2.0	40.0
66-67	30.14642580765326	35.0	29.0	38.0	2.0	40.0
68-69	29.625859001658142	34.0	28.5	37.0	2.0	39.5
70-71	29.094931631232214	34.0	28.0	36.5	2.0	39.0
72-73	28.531206433159618	34.0	26.5	36.0	2.0	39.0
74-75	27.920687231229905	33.5	26.0	35.5	2.0	38.0
76-77	27.31912067791846	33.0	25.5	35.0	2.0	37.0
78-79	26.737695982030623	33.0	24.5	35.0	2.0	36.5
80-81	26.13323969299468	32.0	23.5	35.0	2.0	36.0
82-83	25.486876468870676	32.0	20.5	34.5	2.0	35.5
84-85	24.852207714092806	31.0	18.5	34.0	2.0	35.0
86-87	24.21172648733602	31.0	14.0	34.0	2.0	35.0
88-89	23.543654681600422	30.5	4.5	34.0	2.0	35.0
90-91	22.7794669634792	30.0	2.0	34.0	2.0	35.0
92-93	21.936151343634197	29.0	2.0	34.0	2.0	35.0
94-95	21.001354410098973	29.0	2.0	34.0	2.0	35.0
96-97	20.085230329815197	28.0	2.0	33.0	2.0	34.0
98	19.4516012519269	27.0	2.0	33.0	2.0	34.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	139070.0
3	24296.0
4	18612.0
5	16190.0
6	15204.0
7	15624.0
8	18071.0
9	20710.0
10	23389.0
11	25811.0
12	28817.0
13	32785.0
14	27865.0
15	27989.0
16	30530.0
17	35340.0
18	39471.0
19	39417.0
20	40782.0
21	44400.0
22	48852.0
23	54461.0
24	60736.0
25	68759.0
26	77049.0
27	88567.0
28	101940.0
29	119490.0
30	142376.0
31	171551.0
32	212357.0
33	276349.0
34	368623.0
35	511066.0
36	682667.0
37	710367.0
38	309216.0
39	12944.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.97691973697738	17.63447908329701	14.191004973151081	24.197596206574534
2	15.674811154770918	19.306824158255793	39.05067379926447	25.967690887708812
3	19.7147662840326	23.50032165198751	27.576026347679083	29.20888571630081
4	13.973365442010435	15.603120166681306	35.054387491293745	35.369126900014514
5	15.906806827474561	35.41026788634099	32.40090146124097	16.282023824943476
6	34.02973066960372	33.31558587039764	17.4135099729556	15.241173487043033
7	30.767031400751975	30.084332959731093	20.033506671951944	19.11512896756499
8	27.801474077174237	32.82937660323892	19.52973865990537	19.839410659681477
9	27.07889617727746	16.86530172091515	19.386774173839814	36.669027927967576
10-11	26.316015471495323	25.110566758716175	26.248978449135446	22.324439320653052
12-13	26.656555293811042	22.282546292909025	26.83517411944273	24.22572429383721
14-15	23.968289081685874	23.74085780642471	24.452664717571096	27.83818839431832
16-17	24.230532554541874	26.694133652893758	24.98213260867545	24.093201183888915
18-19	24.205814145090113	26.072758133778386	26.37043690827835	23.350990812853155
20-21	24.87933975251301	25.31626383518996	26.089015318530972	23.715381093766048
22-23	24.600968750587988	24.97945242970208	25.998909118588347	24.420669701121586
24-25	24.36059461555948	25.20897055489964	26.068739398640613	24.36169543090027
26-27	24.414518416852925	25.372479100059962	26.023330115902336	24.18967236718477
28-29	24.381613732576987	25.47341808089571	25.92367246468151	24.22129572184579
30-31	24.601365428132503	25.330596122396116	25.827639062597513	24.240399386873868
32-33	24.460478154550735	25.33382819037766	25.776977374285533	24.428716280786077
34-35	24.586408471576053	25.35292157255496	25.851073594436464	24.209596361432524
36-37	24.65272012215129	25.34776888712344	25.734419385316414	24.265091605408852
38-39	24.628908341759566	25.373288404413557	25.715626320263247	24.28217693356363
40-41	24.73661794900301	25.247565325225718	25.727137043738484	24.28867968203279
42-43	24.648284953565344	25.204263728135462	25.706946974869382	24.440504343429808
44-45	24.662932284319126	25.320795869882357	25.632596860967144	24.383674984831373
46-47	24.762068152179758	25.27320466921759	25.593478237297244	24.371248941305403
48-49	24.717006287747257	25.35616147489721	25.474608231502117	24.45222400585342
50-51	24.602860096577672	25.52776358493266	25.453231912138946	24.416144406350718
52-53	24.692696130287363	25.508759733111408	25.381590618604942	24.416953517996284
54-55	24.594962403810115	25.307839315026303	25.580608940222387	24.51658934094119
56-57	24.66033903444428	25.41896127175121	25.520847481958924	24.39985221184558
58-59	24.705872880919255	25.3290157331547	25.540172360488484	24.424939025437556
60-61	24.742618351325238	25.295446851989972	25.526210772468644	24.435724024216146
62-63	24.608292347913704	25.426937718053814	25.51556586937838	24.449204064654097
64-65	24.624986226350938	25.21590530516929	25.613832201835667	24.5452762666441
66-67	24.6440584124807	25.3744883525993	25.490334465905267	24.49111876901473
68-69	24.62364780895282	25.332972985277486	25.47388024203879	24.569498963730904
70-71	24.594280236720124	25.176808176168127	25.564659313449827	24.664252273661923
72-73	24.705277682995657	25.250042383702343	25.44450310205781	24.60017683124419
74-75	24.54213633094575	25.425658731507372	25.39989049033895	24.632314447207932
76-77	24.68288030008133	25.194106526296817	25.444360277849903	24.67865289577195
78-79	24.671743053692165	25.152502432496544	25.522990780384802	24.65276373342649
80-81	24.605559137082057	25.31322085373221	25.407470610115094	24.673749399070637
82-83	24.73885692057999	25.196238917280322	25.36821858275321	24.696685579386475
84-85	24.681213996656226	25.241338119226402	25.379274731673117	24.69817315244425
86-87	24.69576744975871	25.201352962321756	25.463226326618905	24.639653261300637
88-89	24.669333615465355	25.066425137619834	25.437088243013527	24.827153003901277
90-91	24.671712455074054	25.098976276912786	25.415953550052123	24.813357717961033
92-93	24.69862444097532	25.21763449848481	25.452315441396706	24.63142561914317
94-95	24.72399509172018	25.057064600185157	25.433296672512874	24.78564363558179
96-97	24.73002551424912	25.06284152477839	25.43639321940216	24.77073974157033
98	24.79658190662464	25.031453530733916	25.32571354029442	24.846251022347026
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	616.0
1	485.5
2	624.0
3	825.5
4	588.0
5	477.5
6	800.5
7	1580.0
8	2263.5
9	2253.5
10	1901.0
11	1768.0
12	1938.0
13	2288.0
14	2708.5
15	3023.0
16	3208.0
17	3343.0
18	3409.0
19	3245.5
20	3028.0
21	2864.0
22	2721.0
23	2748.5
24	2915.0
25	3299.5
26	3982.0
27	4994.5
28	6560.0
29	8971.0
30	12508.0
31	17580.0
32	24809.0
33	33830.5
34	44921.5
35	58423.0
36	72794.0
37	86835.5
38	100626.5
39	113330.0
40	125868.0
41	139053.5
42	152496.5
43	166184.5
44	178567.5
45	190654.0
46	203963.0
47	216920.0
48	223260.0
49	221203.0
50	218825.0
51	217303.0
52	214310.5
53	211045.5
54	205216.5
55	195108.5
56	180693.5
57	166224.5
58	151615.0
59	135247.5
60	118266.0
61	98995.0
62	79941.0
63	61598.0
64	45391.5
65	34188.5
66	25996.5
67	19707.0
68	14945.0
69	11197.0
70	8423.0
71	6364.0
72	4808.0
73	3624.0
74	2729.5
75	2016.5
76	1406.0
77	881.0
78	553.0
79	335.5
80	209.5
81	133.0
82	80.5
83	49.5
84	34.5
85	22.5
86	18.0
87	18.5
88	14.5
89	10.5
90	11.0
91	15.0
92	12.0
93	6.5
94	6.0
95	6.5
96	6.0
97	3.5
98	3.5
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.48110287130241874
2	0.05087848692249873
3	0.059230077345125524
4	0.05626109762966485
5	0.06657776815173323
6	0.06706903817659363
7	0.04438517876782216
8	0.021017813237505775
9	0.013435167201617004
10-11	0.015966275807962976
12-13	0.011651643415710773
14-15	0.016414826700226818
16-17	0.015581803614593966
18-19	0.05779898640314088
20-21	0.08634604676078973
22-23	0.10233368213505098
24-25	0.07246232866690888
26-27	0.08753150269034417
28-29	0.0889625936323288
30-31	0.05918735821252896
32-33	0.03425006455928913
34-35	0.4017520825043152
36-37	0.629669334690093
38-39	0.5795170730217357
40-41	0.5737286305549023
42-43	0.5474456842248709
44-45	0.5324192293340322
46-47	0.5227647053672104
48-49	0.5116470511089566
50-51	0.5267803038312867
52-53	0.6085447236210958
54-55	0.57847045427312
56-57	0.5356445238450722
58-59	0.5139752438354689
60-61	0.5254987298533901
62-63	0.5384533068132958
64-65	0.5577837143132376
66-67	0.5829559631957585
68-69	0.536851339340925
70-71	0.48569517805654866
72-73	0.46847936762013637
74-75	0.4478460265759996
76-77	0.462925880382584
78-79	0.45725491553039116
80-81	0.4539655423204563
82-83	0.47376586027896017
84-85	0.4388109300318279
86-87	0.4423886573867895
88-89	0.4696114246339451
90-91	0.45189366438952333
92-93	0.447418835250034
94-95	0.4582588151464102
96-97	0.45303640118648114
98	0.4463935760677167
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	4681743.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.77497730608858
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.6244099840691	42.93574160631862
2	13.48945744919299	15.317272810987593
3	4.60288305526708	7.839857430161046
4	2.049527158232226	4.654474315873873
5	1.1407406011983667	3.2382760872585563
6	0.6634689525877981	2.260106083587996
7	0.4579436667038929	1.8199818899206361
8	0.31978225839117963	1.4524504370439184
9	0.25024559426001564	1.2786919141554938
>10	1.2970704472160193	13.302936784710317
>50	0.07646953668574111	2.931914922446243
>100	0.027246518986822375	2.476456733106137
>500	5.660829941238816E-4	0.21396234367656888
>1k	1.88694214609115E-4	0.27787664075315227
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4912	0.10491818965714264	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.407869889483468E-5
2	0.0	0.0	0.0	0.0	6.407869889483468E-5
3	0.0	0.0	0.0	0.0	6.407869889483468E-5
4	0.0	0.0	0.0	0.0	6.407869889483468E-5
5	0.0	0.0	0.0	0.0	8.54382651931129E-5
6	0.0	0.0	0.0	0.0	1.0679783149139114E-4
7	0.0	0.0	0.0	2.1359566298278226E-5	1.0679783149139114E-4
8	0.0	0.0	0.0	2.1359566298278226E-5	1.0679783149139114E-4
9	0.0	0.0	0.0	2.1359566298278226E-5	1.0679783149139114E-4
10-11	0.0	0.0	0.0	4.271913259655645E-5	1.0679783149139114E-4
12-13	0.0	0.0	0.0	1.0679783149139114E-4	2.0291587983364316E-4
14-15	0.0	2.1359566298278226E-5	0.0	1.0679783149139114E-4	2.7767436187761695E-4
16-17	0.0	4.271913259655645E-5	0.0	2.0291587983364316E-4	2.990339281758952E-4
18-19	0.0	4.271913259655645E-5	0.0	3.417530607724516E-4	2.990339281758952E-4
20-21	0.0	4.271913259655645E-5	0.0	3.951519765181472E-4	3.6311262707072983E-4
22-23	1.0679783149139113E-5	4.271913259655645E-5	0.0	5.980678563517903E-4	4.058317596672863E-4
24-25	4.271913259655645E-5	4.271913259655645E-5	0.0	6.835061215449032E-4	4.058317596672863E-4
26-27	4.271913259655645E-5	4.271913259655645E-5	0.0	7.369050372905988E-4	4.4855089226384276E-4
28-29	4.271913259655645E-5	4.271913259655645E-5	0.0	9.291411339751029E-4	4.4855089226384276E-4
30-31	4.271913259655645E-5	4.271913259655645E-5	0.0	0.0017942035690553708	4.69910458562121E-4
32-33	4.271913259655645E-5	4.271913259655645E-5	0.0	0.003940839982032333	4.69910458562121E-4
34-35	4.271913259655645E-5	4.271913259655645E-5	0.0	0.006439909238930885	4.69910458562121E-4
36-37	4.271913259655645E-5	6.407869889483468E-5	0.0	0.010071035509638185	4.69910458562121E-4
38-39	4.271913259655645E-5	9.611804834225201E-5	0.0	0.016948815857683774	5.126295911586775E-4
40-41	4.271913259655645E-5	1.0679783149139114E-4	0.0	0.02456350124301996	5.126295911586775E-4
42-43	4.271913259655645E-5	1.0679783149139114E-4	0.0	0.03212478771261045	5.126295911586775E-4
44-45	5.339891574569557E-5	1.0679783149139114E-4	0.0	0.040326861171149295	5.233093743078165E-4
46-47	6.407869889483468E-5	1.2815739778966937E-4	0.0	0.048934766389355414	5.339891574569557E-4
48-49	6.407869889483468E-5	1.2815739778966937E-4	0.0	0.05774558748739518	5.339891574569557E-4
50-51	6.407869889483468E-5	1.2815739778966937E-4	0.0	0.06707971795974277	5.339891574569557E-4
52-53	6.407869889483468E-5	1.2815739778966937E-4	0.0	0.0763177303837481	5.66028506904373E-4
54-55	6.407869889483468E-5	1.495169640879476E-4	0.0	0.08650624350802683	6.301072057992077E-4
56-57	6.407869889483468E-5	1.495169640879476E-4	0.0	0.0967695151143495	6.407869889483468E-4
58-59	6.407869889483468E-5	1.495169640879476E-4	0.0	0.10805804590298955	6.407869889483468E-4
60-61	6.407869889483468E-5	1.495169640879476E-4	0.0	0.11991260519853397	6.728263383957642E-4
62-63	6.407869889483468E-5	1.495169640879476E-4	0.0	0.13225843451893876	7.048656878431814E-4
64-65	6.407869889483468E-5	1.495169640879476E-4	0.0	0.14430522991116768	7.048656878431814E-4
66-67	7.47584820439738E-5	1.495169640879476E-4	0.0	0.1567044581473182	7.155454709923206E-4
68-69	8.54382651931129E-5	1.495169640879476E-4	0.0	0.16978719250501362	7.262252541414597E-4
70-71	8.54382651931129E-5	1.601967472370867E-4	0.0	0.18368159038204362	7.796241698871554E-4
72-73	1.0679783149139114E-4	1.708765303862258E-4	0.0	0.19715947671625716	8.543826519311291E-4
74-75	1.2815739778966937E-4	1.708765303862258E-4	0.0	0.2114810659192527	8.757422182294073E-4
76-77	1.495169640879476E-4	1.708765303862258E-4	0.0	0.2260055710020819	8.864220013785464E-4
78-79	1.495169640879476E-4	1.708765303862258E-4	0.0	0.2418650489785535	8.971017845276855E-4
80-81	1.495169640879476E-4	1.9223609668450405E-4	0.0	0.2591128987644132	9.077815676768247E-4
82-83	1.495169640879476E-4	2.0291587983364316E-4	0.0	0.2763287092008254	9.825400497207985E-4
84-85	1.495169640879476E-4	2.1359566298278227E-4	0.0	0.2926153785032626	9.932198328699376E-4
86	1.495169640879476E-4	2.1359566298278227E-4	0.0	0.3059757872228356	0.0010038996160190766
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	10715	0.0	33.808895	1
GTACCTG	6425	0.0	33.314114	1
TACCTGG	6705	0.0	31.38705	2
TCAACGC	13135	0.0	27.052551	4
TATCAAC	13335	0.0	26.921879	2
CAACGCA	13465	0.0	26.355495	5
ATCAACG	13655	0.0	26.08951	3
GTACATG	14525	0.0	25.289206	1
AACGCAG	14290	0.0	25.14886	6
ACCTGGG	8375	0.0	24.96414	3
GGTATCA	6880	0.0	23.818258	1
TACATGG	15270	0.0	22.999918	2
ACATGGG	15480	0.0	22.214006	3
ACGCAGA	16285	0.0	21.865578	7
CGCAGAG	17260	0.0	20.572695	8
TATAACG	320	6.384653E-10	18.626446	2
CATGGGG	12005	0.0	18.370438	4
AGAGTAC	14405	0.0	18.066622	10-11
GCAGAGT	19305	0.0	17.871462	9
CCTGGGG	8685	0.0	17.685265	4
>>END_MODULE