FastQCFastQC Report
Mon 15 Aug 2016
SRR2921463_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921463_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4681743
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47430.10130842295273362No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG67200.034.585541
GTATCAA102300.033.7525331
TACCTGG70650.032.340122
TCAACGC126500.026.7378224
TATCAAC129000.026.2179642
CAACGCA130600.025.921215
ATCAACG132450.025.4994583
GTACATG143350.024.8844551
ACCTGGG89500.024.7860413
AACGCAG138600.024.6311046
TACATGG149500.023.1312372
GGTATCA66100.022.1197851
ACATGGG154250.021.4645083
ACGCAGA162400.020.9345557
TATAACG4350.020.7478472
CGCAGAG172100.019.6718628
GTATAGG11350.019.3021621
CATGGGG120400.018.7809284
TATCACG4900.018.4190062
GAGTACA118550.018.2388841
Produced by FastQC (version 0.11.5)