##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921463_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4681743
Sequences flagged as poor quality	0
Sequence length	101
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.946785203715795	33.0	31.0	34.0	30.0	34.0
2	32.0654256331456	34.0	31.0	34.0	30.0	34.0
3	32.190739431873986	34.0	31.0	34.0	30.0	34.0
4	35.73018467694617	37.0	35.0	37.0	35.0	37.0
5	35.53597004363546	37.0	35.0	37.0	33.0	37.0
6	35.568262290347846	37.0	35.0	37.0	33.0	37.0
7	35.50299877631045	37.0	35.0	37.0	33.0	37.0
8	35.4561042756939	37.0	35.0	37.0	33.0	37.0
9	37.07963615260385	39.0	37.0	39.0	34.0	39.0
10-11	36.98920241884272	39.0	37.0	39.0	33.5	39.0
12-13	36.878459582253875	39.0	37.0	39.0	33.0	39.0
14-15	38.182445619078194	40.0	38.0	41.0	33.0	41.0
16-17	38.08975204320271	40.0	38.0	41.0	33.0	41.0
18-19	37.983494822334336	40.0	38.0	41.0	33.0	41.0
20-21	37.871610210129006	40.0	38.0	41.0	33.0	41.0
22-23	37.83556989352043	40.0	38.0	41.0	33.0	41.0
24-25	37.89541587395976	40.0	38.0	41.0	33.0	41.0
26-27	37.793335943472336	40.0	38.0	41.0	33.0	41.0
28-29	37.65765538603892	40.0	38.0	41.0	32.5	41.0
30-31	37.52295170837015	40.0	38.0	41.0	32.0	41.0
32-33	37.366964718054795	40.0	37.0	41.0	32.0	41.0
34-35	37.22734470901115	40.0	37.0	41.0	31.0	41.0
36-37	37.13431899187974	40.0	37.0	41.0	31.0	41.0
38-39	37.1064105825544	40.0	37.0	41.0	31.0	41.0
40-41	36.94801882546735	40.0	37.0	41.0	31.0	41.0
42-43	36.72009367024204	40.0	36.0	41.0	30.0	41.0
44-45	36.52364973899678	39.0	36.0	41.0	30.0	41.0
46-47	36.264487179240724	39.0	35.5	40.5	30.0	41.0
48-49	35.94318825275117	39.0	35.0	40.0	29.0	41.0
50-51	35.677845516082364	39.0	35.0	40.0	28.0	41.0
52-53	35.37328960602921	38.0	35.0	40.0	28.0	41.0
54-55	35.053974769653095	38.0	34.0	40.0	27.0	41.0
56-57	34.68130726099233	38.0	34.0	40.0	26.0	41.0
58-59	34.29845390915307	37.0	33.5	40.0	26.0	41.0
60-61	33.880527295069385	37.0	33.0	39.5	25.0	41.0
62-63	33.44281168359733	36.0	32.5	39.0	23.5	40.0
64-65	32.93019234503047	35.5	32.0	39.0	22.5	40.0
66-67	32.40543650089293	35.0	31.0	38.0	21.5	40.0
68-69	31.9059874709056	35.0	31.0	37.5	20.0	40.0
70-71	31.352105188174576	34.5	30.5	37.0	18.5	39.0
72-73	30.795156953297095	34.0	30.0	36.0	17.0	39.0
74-75	30.267006540085603	34.0	29.0	36.0	13.0	38.5
76-77	28.97547750912427	32.5	28.0	34.5	11.5	36.0
78-79	29.912239843152435	34.0	29.5	35.0	11.0	37.0
80-81	29.940068154104146	34.0	30.0	35.0	9.5	36.5
82-83	29.6011981007928	34.0	29.5	35.0	7.0	36.0
84-85	29.10309557786491	34.0	29.0	35.0	3.5	36.0
86-87	28.575868645502325	33.5	29.0	35.0	2.0	35.0
88-89	28.029704321659686	33.0	27.5	35.0	2.0	35.0
90-91	27.55231651972353	33.0	26.5	35.0	2.0	35.0
92-93	27.114220921567032	33.0	26.0	34.0	2.0	35.0
94-95	26.618683682551563	32.0	25.0	34.0	2.0	35.0
96-97	25.843783180751274	32.0	24.0	34.0	2.0	35.0
98-99	24.813638745228005	32.0	18.5	34.0	2.0	35.0
100-101	21.192541431684738	27.5	2.0	32.5	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	30005.0
3	15354.0
4	8951.0
5	6868.0
6	6071.0
7	6179.0
8	7088.0
9	8738.0
10	10254.0
11	12196.0
12	14268.0
13	16853.0
14	18792.0
15	20875.0
16	23473.0
17	27563.0
18	27819.0
19	25728.0
20	25956.0
21	27449.0
22	29561.0
23	32778.0
24	37463.0
25	44084.0
26	52481.0
27	63380.0
28	77195.0
29	94681.0
30	115598.0
31	145063.0
32	187499.0
33	250856.0
34	356307.0
35	536280.0
36	832325.0
37	1026939.0
38	446166.0
39	12607.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.17868392294671	18.12303670530759	14.571642846429105	24.12663652531659
2	15.79550607540824	19.61681792443541	38.65158766724273	25.93608833291362
3	19.69221981698864	23.718166434474046	27.8114425035961	28.778171244941213
4	14.225391727190228	15.833485434805747	35.113917947275574	34.82720489072845
5	15.928241787957509	35.411927491636625	32.471855441224406	16.18797527918146
6	34.0417050764623	33.41031322515967	17.39455589370662	15.153425804671414
7	30.797561958897706	30.087307419848138	20.04216595250035	19.0729646687538
8	27.76704389218389	32.95189551439046	19.537168537826684	19.74389205559896
9	26.919092892158275	16.895440494111273	19.4840557438041	36.70141086992635
10-11	26.26455795472816	25.189345347441016	26.339282659934387	22.206814037896436
12-13	26.60700289107436	22.290371452383223	26.926117891350167	24.17650776519225
14-15	23.892032345027303	23.744918170278407	24.597926038524978	27.765123446169316
16-17	24.169740613285068	26.739793957703732	25.108906224142284	23.981559204868912
18-19	24.207460466374503	26.14201752021434	26.407909470879616	23.242612542531546
20-21	24.833205548302477	25.31891329914741	26.207301469654272	23.640579682895847
22-23	24.564102525748485	24.98346329599616	26.150343988017287	24.302090190238072
24-25	24.262250806895235	25.276998152081443	26.21200413108139	24.248746909941936
26-27	24.34725501326395	25.376922802475594	26.152185993091255	24.123636191169204
28-29	24.32492253918708	25.471978173703434	26.093384544798546	24.109714742310935
30-31	24.51650152838309	25.342718053717533	25.977930748402013	24.16284966949737
32-33	24.366060819669066	25.306600234435283	25.955542739047893	24.371796206847755
34-35	24.500969063659994	25.356977076779362	26.017175059272773	24.124878800287867
36-37	24.531313335223878	25.38450405532351	25.909797556925934	24.17438505252668
38-39	24.556388410650566	25.396046488823963	25.889685284171833	24.157879816353635
40-41	24.620196962088436	25.28013414948485	25.877527921015197	24.222140967411516
42-43	24.592727874700287	25.274015689164436	25.842414144812377	24.290842291322896
44-45	24.530872249380003	25.2411462224217	25.826246117082786	24.40173541111551
46-47	24.596607194342667	25.309681138910012	25.769933878928054	24.323777787819267
48-49	24.581079929366346	25.431682744075186	25.635853478955262	24.351383847603213
50-51	24.536019271940603	25.553285943840727	25.560805033945382	24.349889750273288
52-53	24.62756003415052	25.513309795932056	25.51638564968973	24.34274452022769
54-55	24.590998392093223	25.35887400600549	25.61955676028412	24.43057084161718
56-57	24.624730111561007	25.431778781620846	25.559909038945104	24.383582067873043
58-59	24.644385515044263	25.392289314637782	25.556396895562173	24.406928274755778
60-61	24.75286968084396	25.323211906472036	25.594664089138337	24.32925432354567
62-63	24.56588987728479	25.499378717321775	25.57110420832629	24.363627197067146
64-65	24.660036704639797	25.277156557795603	25.615184010764246	24.447622726800358
66-67	24.610976256004378	25.415049829911624	25.546515752406023	24.42745816167798
68-69	24.643054422051495	25.36961434478431	25.544871853099156	24.442459380065035
70-71	24.62587870679311	25.232660901582488	25.625575577382488	24.51588481424191
72-73	24.745974673959168	25.290134569964547	25.461578696144343	24.502312059931945
74-75	24.576405477069088	25.463060664165948	25.395518907364362	24.565014951400602
76-77	24.658148526894017	25.233644659579713	25.537750690901735	24.570456122624535
78-79	24.61094216282919	25.265316016175998	25.612013280923858	24.511728540070955
80-81	24.568532929698424	25.432524731647817	25.496080563453592	24.50286177520017
82-83	24.659736951126	25.38470347658748	25.410993835386247	24.544565736900275
84-85	24.67074883210289	25.35553913403316	25.41160802239081	24.56210401147313
86-87	24.63184626200577	25.381139883076575	25.487295871860056	24.499717983057597
88-89	24.631268404907317	25.19766822144338	25.50916396359409	24.661899410055216
90-91	24.61672679550236	25.298009888106463	25.44251800229108	24.64274531410009
92-93	24.6881067425617	25.4313785682513	25.48664152622357	24.393873162963434
94-95	24.66705957595703	25.2690588419757	25.44509900844164	24.618782573625634
96-97	24.650418587721425	25.302875857425555	25.515160240745615	24.531545314107404
98-99	24.66669821107933	25.275961096126327	25.433376972144394	24.623963720649943
100-101	25.117129298826303	24.78471556907013	25.5624801740518	24.535674958051764
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	251.0
1	228.0
2	423.5
3	633.5
4	449.0
5	313.0
6	563.0
7	1280.0
8	1930.5
9	1868.5
10	1333.5
11	943.5
12	902.5
13	950.5
14	978.0
15	1055.5
16	1177.5
17	1273.5
18	1389.0
19	1491.5
20	1573.5
21	1708.5
22	1848.0
23	2049.0
24	2325.0
25	2667.0
26	3268.5
27	4124.0
28	5347.5
29	7210.0
30	10168.0
31	14520.0
32	20781.0
33	28627.5
34	37910.5
35	49270.0
36	62485.5
37	76734.5
38	90489.0
39	103070.0
40	116206.0
41	128363.0
42	140565.0
43	154571.0
44	167965.5
45	180494.0
46	192064.0
47	204107.0
48	217844.5
49	223906.5
50	218465.5
51	213912.0
52	211050.5
53	206908.0
54	202355.0
55	194011.0
56	180255.5
57	163404.0
58	147858.5
59	133001.0
60	116133.5
61	98225.5
62	80433.0
63	61604.0
64	45201.0
65	34064.0
66	25783.5
67	19692.0
68	15082.0
69	11325.5
70	8518.5
71	6480.5
72	4849.0
73	3620.5
74	2731.0
75	1940.0
76	1291.0
77	809.0
78	528.5
79	293.5
80	152.0
81	88.5
82	52.0
83	24.5
84	13.5
85	4.5
86	3.0
87	3.5
88	1.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.31704004256534374
2	0.0
3	0.006087476395009294
4	0.06085340438379467
5	4.4855089226384276E-4
6	0.06476220501637958
7	0.07606141558816876
8	0.10605024667095139
9	0.09432384477319665
10-11	0.09772001581462289
12-13	0.09801904974279878
14-15	0.0654457111379245
16-17	0.03598018942944967
18-19	0.03918412437419141
20-21	0.028974251683614415
22-23	0.04275117194600387
24-25	0.034442300655973646
26-27	0.02157316196126101
28-29	0.024574181026169102
30-31	0.0034495699571719333
32-33	0.005991358346667043
34-35	0.008971017845276855
36-37	0.011758441247202165
38-39	0.0168740573756398
40-41	0.021242088683637694
42-43	0.003588407138110742
44-45	0.014140032889460186
46-47	0.024830495821748438
48-49	0.002605867088389944
50-51	0.0069525388300895625
52-53	0.0024777096906002743
54-55	0.004068997379822002
56-57	0.003823362367391802
58-59	0.0012388548453001371
60-61	0.005457369189210087
62-63	0.014983735758242175
64-65	0.006803021866001615
66-67	0.03893848936176121
68-69	0.06223109641003362
70-71	0.012260391055211702
72-73	0.03206070901371562
74-75	0.05159403239349106
76-77	0.3052709215349924
78-79	0.073775941994253
80-81	0.03430346347503484
82-83	0.02819462751372726
84-85	0.019095452270660736
86-87	0.1197310488849986
88-89	0.5275172088685773
90-91	0.13300601933937853
92-93	0.04907360357029423
94-95	0.009077815676768245
96-97	0.006130195527605851
98-99	0.01094677772786759
100-101	0.00807391606074917
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4681743.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.021998521601276
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.26482594108975	38.31625494212234
2	15.234585658023539	16.155363564738643
3	5.292982601078744	8.419335469477755
4	2.3430600113861577	4.969348978389598
5	1.2695608398602516	3.365732648707657
6	0.7700257633546166	2.4496982931710027
7	0.527081963569257	1.9562857365192292
8	0.3803713888173758	1.6134440972427457
9	0.2691522324831212	1.2843890345525162
>10	1.5185541299773297	14.590054016013173
>50	0.09547060222899947	3.4540303905339713
>100	0.03332028155740626	2.860679952221296
>500	7.665260359286931E-4	0.25964289835386106
>1k	2.420605375118199E-4	0.3057399779560324
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4743	0.10130842295273362	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.271913259655645E-5
2	0.0	0.0	0.0	0.0	4.271913259655645E-5
3	0.0	0.0	0.0	0.0	4.271913259655645E-5
4	0.0	0.0	0.0	0.0	4.271913259655645E-5
5	0.0	2.1359566298278226E-5	0.0	0.0	6.407869889483468E-5
6	0.0	2.1359566298278226E-5	0.0	0.0	8.54382651931129E-5
7	0.0	2.1359566298278226E-5	0.0	0.0	8.54382651931129E-5
8	0.0	2.1359566298278226E-5	0.0	0.0	8.54382651931129E-5
9	0.0	2.1359566298278226E-5	0.0	8.54382651931129E-5	8.54382651931129E-5
10-11	0.0	2.1359566298278226E-5	0.0	1.0679783149139114E-4	1.0679783149139114E-4
12-13	0.0	2.1359566298278226E-5	0.0	1.8155631353536492E-4	1.495169640879476E-4
14-15	0.0	2.1359566298278226E-5	0.0	2.1359566298278227E-4	1.9223609668450402E-4
16-17	1.0679783149139113E-5	2.1359566298278226E-5	0.0	2.7767436187761695E-4	2.349552292810605E-4
18-19	2.1359566298278226E-5	2.1359566298278226E-5	0.0	4.058317596672863E-4	2.5631479557933874E-4
20-21	2.1359566298278226E-5	2.1359566298278226E-5	0.0	4.3787110911470365E-4	2.5631479557933874E-4
22-23	2.1359566298278226E-5	2.1359566298278226E-5	0.0	5.446689406060949E-4	2.5631479557933874E-4
24-25	2.1359566298278226E-5	2.1359566298278226E-5	0.0	6.62146555246625E-4	2.6699457872847785E-4
26-27	2.1359566298278226E-5	2.1359566298278226E-5	0.0	7.262252541414597E-4	2.7767436187761695E-4
28-29	2.1359566298278226E-5	2.1359566298278226E-5	0.0	9.291411339751029E-4	2.7767436187761695E-4
30-31	2.1359566298278226E-5	2.1359566298278226E-5	0.0	0.00168740573756398	2.7767436187761695E-4
32-33	2.1359566298278226E-5	3.203934944741734E-5	0.0	0.0037913230179443856	2.7767436187761695E-4
34-35	2.1359566298278226E-5	6.407869889483468E-5	0.0	0.006279712491693798	2.7767436187761695E-4
36-37	2.1359566298278226E-5	9.611804834225203E-5	0.0	0.009889479196102818	2.8835414502675606E-4
38-39	2.1359566298278226E-5	1.495169640879476E-4	0.0	0.016671141495806157	3.203934944741734E-4
40-41	2.1359566298278226E-5	1.708765303862258E-4	0.0	0.024168349266501812	3.203934944741734E-4
42-43	2.1359566298278226E-5	1.9223609668450405E-4	0.0	0.03175099530239058	3.203934944741734E-4
44-45	2.1359566298278226E-5	1.9223609668450405E-4	0.0	0.039921029411482005	3.524328439215908E-4
46-47	2.1359566298278226E-5	1.9223609668450405E-4	0.0	0.048422136798196735	4.058317596672863E-4
48-49	2.1359566298278226E-5	1.9223609668450405E-4	0.0	0.05720091854678909	4.2719132596556454E-4
50-51	2.1359566298278226E-5	2.1359566298278227E-4	0.0	0.06621465552466249	4.4855089226384276E-4
52-53	2.1359566298278226E-5	2.349552292810605E-4	0.0	0.07540994881607128	5.019498080095384E-4
54-55	2.1359566298278226E-5	2.7767436187761695E-4	0.0	0.08536350671106893	5.446689406060949E-4
56-57	2.1359566298278226E-5	2.7767436187761695E-4	0.0	0.09531706460606659	5.66028506904373E-4
58-59	2.1359566298278226E-5	2.7767436187761695E-4	0.0	0.10626384233393418	5.767082900535121E-4
60-61	4.271913259655645E-5	2.990339281758952E-4	0.0	0.11789412618334667	5.873880732026513E-4
62-63	4.271913259655645E-5	2.990339281758952E-4	0.0	0.1300263598407687	6.62146555246625E-4
64-65	4.271913259655645E-5	3.097137113250343E-4	0.0	0.14157120542498808	7.689443867380162E-4
66-67	4.271913259655645E-5	3.417530607724516E-4	0.0	0.15355392211832217	7.689443867380162E-4
68-69	4.271913259655645E-5	3.417530607724516E-4	0.0	0.16634830233099085	7.689443867380162E-4
70-71	4.271913259655645E-5	3.417530607724516E-4	0.0	0.17984754823150267	7.903039530362944E-4
72-73	4.271913259655645E-5	3.417530607724516E-4	0.0	0.19301572085439117	9.291411339751029E-4
74-75	4.271913259655645E-5	3.417530607724516E-4	0.0	0.20712371439440397	9.825400497207985E-4
76-77	4.271913259655645E-5	3.417530607724516E-4	0.0	0.22124238771756588	9.932198328699376E-4
78-79	4.271913259655645E-5	3.524328439215907E-4	0.0	0.23693098916365124	0.0010145793991682157
80-81	4.271913259655645E-5	3.6311262707072983E-4	0.0	0.2536341700089048	0.001025259182317355
82-83	4.271913259655645E-5	3.6311262707072983E-4	0.0	0.2705509465171412	0.0011106974475104678
84-85	4.271913259655645E-5	3.6311262707072983E-4	0.0	0.28682693603642917	0.001132057013808746
86-87	5.339891574569557E-5	3.6311262707072983E-4	0.0	0.304395179316763	0.001153416580107024
88-89	6.407869889483468E-5	3.6311262707072983E-4	0.0	0.3226255691523435	0.0011747761464053024
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCTG	6720	0.0	34.58554	1
GTATCAA	10230	0.0	33.752533	1
TACCTGG	7065	0.0	32.34012	2
TCAACGC	12650	0.0	26.737822	4
TATCAAC	12900	0.0	26.217964	2
CAACGCA	13060	0.0	25.92121	5
ATCAACG	13245	0.0	25.499458	3
GTACATG	14335	0.0	24.884455	1
ACCTGGG	8950	0.0	24.786041	3
AACGCAG	13860	0.0	24.631104	6
TACATGG	14950	0.0	23.131237	2
GGTATCA	6610	0.0	22.119785	1
ACATGGG	15425	0.0	21.464508	3
ACGCAGA	16240	0.0	20.934555	7
TATAACG	435	0.0	20.747847	2
CGCAGAG	17210	0.0	19.671862	8
GTATAGG	1135	0.0	19.302162	1
CATGGGG	12040	0.0	18.780928	4
TATCACG	490	0.0	18.419006	2
GAGTACA	11855	0.0	18.238884	1
>>END_MODULE