FastQCFastQC Report
Mon 15 Aug 2016
SRR2921462_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921462_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3695185
Sequences flagged as poor quality0
Sequence length98
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT81840.2214774091148346No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53290.14421470102308817No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG115800.025.3614561
TACATGG118450.024.5351662
GTACCTG36750.022.6716581
ACATGGG123700.022.5674463
GAGTACA88400.021.14151
AGTACAT83750.020.6343772
TACCTGG38200.020.399542
GTGTAGC19550.019.0721781
CATGGGG94050.018.6670114
GTATCAA162650.018.339311
TCGCGTA1550.001228061417.7328289
AGAGTAC112100.017.63306210-11
GTACTTT60550.016.73042714-15
GAGTACT62100.016.27053612-13
TATCAAC181750.016.2674752
TCAACGC181950.016.2495944
GTATAGG8000.016.1112821
CAACGCA183550.016.1079445
ATCAACG184950.016.0603683
GTACACG8650.015.9649371
Produced by FastQC (version 0.11.5)