##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921462_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3695185
Sequences flagged as poor quality	0
Sequence length	98
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.121426126161477	31.0	31.0	34.0	26.0	34.0
2	30.21342936821837	31.0	31.0	34.0	26.0	34.0
3	30.347585303577493	31.0	31.0	34.0	26.0	34.0
4	33.84602584173729	37.0	35.0	37.0	30.0	37.0
5	33.822591291099094	37.0	35.0	37.0	30.0	37.0
6	33.92499590683552	37.0	35.0	37.0	30.0	37.0
7	33.803785466762825	37.0	35.0	37.0	30.0	37.0
8	33.78092680068792	37.0	35.0	37.0	29.0	37.0
9	35.201326320603705	39.0	35.0	39.0	29.0	39.0
10-11	35.065510387166	39.0	35.0	39.0	29.0	39.0
12-13	34.894213550877694	39.0	35.0	39.0	27.5	39.0
14-15	35.85595741485203	39.5	36.0	41.0	27.0	41.0
16-17	35.70098574225648	39.0	36.0	41.0	27.0	41.0
18-19	35.52076269523718	39.0	36.0	40.5	27.0	41.0
20-21	35.397637466053794	39.0	36.0	40.0	27.0	41.0
22-23	35.438622829438856	39.0	36.0	41.0	26.5	41.0
24-25	35.29002891600827	39.0	35.5	41.0	25.5	41.0
26-27	34.999026976998444	39.0	35.0	40.0	24.5	41.0
28-29	34.75179578289044	39.0	34.5	40.0	24.0	41.0
30-31	34.48383734508556	38.0	34.0	40.0	22.5	41.0
32-33	34.18775825838219	38.0	34.0	40.0	20.0	41.0
34-35	33.746922278586865	38.0	33.0	40.0	17.0	41.0
36-37	33.34507473915379	38.0	33.0	40.0	15.0	41.0
38-39	33.09049979906284	38.0	32.5	40.0	11.5	41.0
40-41	32.81603695078866	38.0	32.0	40.0	9.0	41.0
42-43	32.50849740946664	37.5	31.0	40.0	8.5	41.0
44-45	32.17863354608768	37.0	31.0	40.0	8.0	41.0
46-47	31.856597166312376	37.0	30.5	40.0	6.5	41.0
48-49	31.610740192980863	37.0	30.0	40.0	2.0	41.0
50-51	30.895242457414177	36.0	29.5	38.5	2.0	39.5
52-53	31.45374602354145	36.5	30.5	39.0	2.0	40.0
54-55	31.881132203123798	37.0	31.0	39.5	2.0	40.5
56-57	31.722719972071765	37.0	31.0	40.0	2.0	41.0
58-59	31.382748766299926	36.0	31.0	39.0	2.0	41.0
60-61	30.902829763597765	36.0	30.0	39.0	2.0	40.0
62-63	30.407717746202152	35.0	29.0	39.0	2.0	40.0
64-65	29.85521983337776	35.0	28.5	38.0	2.0	40.0
66-67	29.346532852888288	34.5	28.0	38.0	2.0	40.0
68-69	28.807521003684524	34.0	26.5	37.0	2.0	39.5
70-71	28.25842062034783	34.0	26.0	36.5	2.0	39.0
72-73	27.680259445738173	33.5	26.0	36.0	2.0	39.0
74-75	27.06122291576741	33.0	24.5	35.0	2.0	38.0
76-77	26.450951711484	32.5	23.5	35.0	2.0	37.0
78-79	25.852892886283094	32.0	21.0	35.0	2.0	36.5
80-81	25.22838897105287	32.0	19.0	35.0	2.0	36.0
82-83	24.559490120251084	31.0	14.5	34.0	2.0	35.5
84-85	23.905293645649678	31.0	7.0	34.0	2.0	35.0
86-87	23.255295201728735	30.0	2.0	34.0	2.0	35.0
88-89	22.559543297561554	30.0	2.0	34.0	2.0	35.0
90-91	21.768840125731188	29.0	2.0	34.0	2.0	35.0
92-93	20.91265457615789	29.0	2.0	34.0	2.0	35.0
94-95	19.972951286606758	27.5	2.0	33.0	2.0	34.5
96-97	19.059109489781974	26.0	2.0	33.0	2.0	34.0
98	18.43079954048309	25.0	2.0	32.0	2.0	34.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	133995.0
3	22587.0
4	17034.0
5	14470.0
6	13540.0
7	14253.0
8	16117.0
9	18704.0
10	20981.0
11	22948.0
12	24977.0
13	27885.0
14	24208.0
15	24643.0
16	27652.0
17	31889.0
18	35515.0
19	34810.0
20	35779.0
21	39215.0
22	42734.0
23	47530.0
24	52401.0
25	58347.0
26	65906.0
27	74969.0
28	85573.0
29	99836.0
30	117682.0
31	140390.0
32	172244.0
33	219083.0
34	287432.0
35	389615.0
36	504576.0
37	509513.0
38	216758.0
39	9394.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.16939235975613	17.091929901658585	13.053930189370384	24.684747549214897
2	15.908821994837682	18.754567388594978	39.1268195433735	26.209791073193834
3	21.054176462209917	21.849355240690105	27.401471863300518	29.69499643379946
4	13.752604854886297	14.340673861337239	35.099796810487874	36.806924473288596
5	15.330264938790078	35.920580647991635	32.958440729850395	15.790713683367896
6	35.19719017373208	33.224137534171646	16.368023332173404	15.210648959922876
7	31.416025999932856	30.257383957105745	18.922726873997576	19.403863168963824
8	27.771380746433405	33.73783503887753	18.58786029316629	19.90292392152277
9	26.591826493929982	17.027280566473543	19.06375533937908	37.31713760021739
10-11	26.793453827555062	25.012809176160882	25.80838555119516	22.385351445088897
12-13	27.051895413179	21.950790425924797	26.463113795160886	24.534200365735312
14-15	23.73969311019061	23.703803047461495	24.40252248136207	28.153981360985824
16-17	24.021857515286943	26.9704676159296	24.92154885326266	24.086126015520794
18-19	24.01276540495109	26.273030577374364	26.422010846424275	23.292193171250275
20-21	24.560692637268865	25.287216380891454	26.352902810876444	23.799188170963237
22-23	24.219083015218967	25.018370334290648	26.359076272544378	24.40347037794601
24-25	24.10956073527056	25.251726755973625	26.469084808839867	24.169627699915953
26-27	24.13470687768032	25.295012915914246	26.623170313197036	23.9471098932084
28-29	23.95345482912242	25.77285799510956	26.33802807363964	23.935659102128376
30-31	24.245586666939257	25.39052752847256	26.25339308805843	24.110492716529755
32-33	24.01873033667258	25.50045676649926	26.276560508329883	24.204252388498283
34-35	24.330681306683452	25.443002667152882	26.371961512892916	23.854354513270753
36-37	24.295004081308043	25.363484263200654	26.357133655280496	23.984378000210807
38-39	24.155019927921384	25.577563999783315	26.348574883441874	23.91884118885343
40-41	24.269913594136064	25.586661872238658	26.20550511758008	23.9379194160452
42-43	24.161918074349494	25.471553960196047	26.07865353375139	24.287874431703067
44-45	24.233871077456374	25.3586034071337	26.14001028507059	24.267515230339328
46-47	24.4192419453067	25.576439038411497	26.02794287907122	23.97637613721059
48-49	24.366775721826777	25.615807099543968	25.931057352640536	24.086359825988715
50-51	24.03154125335453	25.74558069978279	26.079581481457804	24.14329656540488
52-53	24.23188884091931	25.927166388039208	25.818942119689858	24.022002651351617
54-55	24.063023887322363	25.539956546232613	26.06674806818836	24.330271498256664
56-57	24.24047956708138	25.592942107365474	25.967892993697124	24.198685331856023
58-59	24.487689588176696	25.5900172851207	25.89611136470843	24.02618176199417
60-61	24.349092652345668	25.553443502598515	26.1284863101014	23.968977534954416
62-63	24.084666876824567	25.725313464749593	26.078142004609777	24.11187765381606
64-65	24.0849874917537	25.730001502295902	26.010647794346575	24.17436321160382
66-67	24.183251807899154	25.65311883956892	25.84506669378056	24.318562658751368
68-69	24.0571106945208	25.683295857892368	25.937331593504187	24.322261854082647
70-71	24.257996316055564	25.478870623276293	25.988764866929543	24.2743681937386
72-73	24.347890754018714	25.39491728595083	26.040035070827543	24.217156889202915
74-75	24.064664166429814	25.87867476300829	25.856274032700988	24.200387037860903
76-77	24.162371624553682	25.479881910359325	25.900558582054245	24.457187883032745
78-79	24.173317819979573	25.400955351801187	26.05822020833189	24.36750661988735
80-81	24.273723184866988	25.590091628835182	25.857921815552366	24.278263370745464
82-83	24.345864089180512	25.49575321177381	25.88104370242163	24.27733899662405
84-85	24.252619572577526	25.53370724667543	25.881801123153554	24.331872057593493
86-87	24.217219958041873	25.629443269879648	25.885011352616132	24.268325419462347
88-89	24.18927559565333	25.398997694569708	25.79834547954432	24.61338123023264
90-91	24.281515136356617	25.268629822069784	25.854308625515728	24.59554641605787
92-93	24.38498850398164	25.537635102852498	25.900940546249473	24.176435846916398
94-95	24.46758573569273	25.31264329158503	25.854400184228883	24.36537078849336
96-97	24.18622461671384	25.40809410544179	26.020870754816748	24.38481052302762
98	24.37915303672737	25.522260127340534	25.59117440531871	24.507412430613382
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	642.0
1	453.0
2	447.0
3	650.5
4	575.5
5	555.5
6	941.5
7	1860.5
8	2641.5
9	2606.5
10	2069.5
11	1743.5
12	1862.5
13	2066.5
14	2328.5
15	2668.5
16	2943.5
17	3047.5
18	3071.5
19	3064.0
20	2949.0
21	2816.5
22	2854.5
23	3131.0
24	3460.0
25	4162.5
26	5429.0
27	7172.0
28	9392.0
29	12368.0
30	16347.0
31	21730.0
32	28455.5
33	35710.5
34	43755.5
35	52664.0
36	61752.5
37	70690.0
38	79629.5
39	87764.5
40	96134.0
41	105967.5
42	116257.5
43	127189.0
44	137045.5
45	146256.5
46	161037.5
47	176645.0
48	180194.5
49	172378.0
50	166931.0
51	164241.0
52	160747.0
53	159542.5
54	156225.0
55	148134.0
56	137146.5
57	126126.0
58	115283.0
59	103387.0
60	89748.0
61	75002.5
62	60662.0
63	46753.0
64	34877.0
65	26533.5
66	20368.0
67	15518.5
68	11889.0
69	9205.0
70	6909.0
71	5251.5
72	4081.5
73	3000.5
74	2280.0
75	1744.5
76	1237.5
77	768.5
78	475.5
79	293.5
80	171.0
81	103.0
82	59.5
83	35.0
84	25.5
85	19.5
86	13.5
87	11.0
88	10.0
89	10.0
90	10.0
91	5.0
92	4.5
93	4.5
94	4.5
95	4.5
96	3.0
97	2.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.4842518033603189
2	0.050931144178167
3	0.05896863079926986
4	0.05631653083675107
5	0.06719555313198122
6	0.06576125417265982
7	0.04430089427187001
8	0.020648492565324875
9	0.012638068188737507
10-11	0.01537135488480279
12-13	0.011812669731014821
14-15	0.01539841712931829
16-17	0.014315927348698373
18-19	0.056248875225462326
20-21	0.08528666359059153
22-23	0.10206525519020021
24-25	0.07143079439865663
26-27	0.08735692529602712
28-29	0.08896712884469925
30-31	0.05952340681183757
32-33	0.03476145308015702
34-35	0.40711087536889223
36-37	0.6378029787412538
38-39	0.5871424570082417
40-41	0.5811887632148323
42-43	0.5547624814454486
44-45	0.54002708930676
46-47	0.5298652164911906
48-49	0.5200416217320649
50-51	0.5343304868362477
52-53	0.6148136020253383
54-55	0.5852345687698992
56-57	0.5422461933570308
58-59	0.5193515344969196
60-61	0.5336539307233603
62-63	0.5458590029998498
64-65	0.5631382461229951
66-67	0.5897269013594718
68-69	0.5442623305734354
70-71	0.49112561346725536
72-73	0.4731698142312225
74-75	0.45284606860008364
76-77	0.4682038923626287
78-79	0.4617766092901979
80-81	0.4579337705689972
82-83	0.47900172792431234
84-85	0.444795050856723
86-87	0.4470006237847361
88-89	0.47530773154794687
90-91	0.4570542476222435
92-93	0.4529543175781456
94-95	0.46415808680756176
96-97	0.45810967515834794
98	0.45199360789784543
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	3695185.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.32956958940273
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.4142426703013	43.234660731991426
2	13.751220366843725	15.767030899203588
3	4.747792826041441	8.165667576498295
4	2.089907440782676	4.7925397624704384
5	1.099031399056314	3.1503498536568793
6	0.6644226344184868	2.285463819400136
7	0.43890417488825406	1.7613531206134883
8	0.3082049984695871	1.4135407926051162
9	0.21616166018613384	1.1153209446181607
>10	1.1793751448651169	12.010616145610168
>50	0.06613539721765858	2.579862654575894
>100	0.022892199735352753	2.1413873132525527
>500	9.020182504580354E-4	0.3627483009067798
>1k	7.121196560565382E-4	0.8516746430803057
>5k	9.494928747420494E-5	0.3677834415168145
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	8184	0.2214774091148346	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5329	0.14421470102308817	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.7062244515497872E-5
2	0.0	0.0	0.0	2.7062244515497872E-5	2.7062244515497872E-5
3	0.0	0.0	0.0	2.7062244515497872E-5	2.7062244515497872E-5
4	0.0	0.0	0.0	2.7062244515497872E-5	2.7062244515497872E-5
5	0.0	0.0	0.0	2.7062244515497872E-5	2.7062244515497872E-5
6	0.0	0.0	0.0	2.7062244515497872E-5	2.7062244515497872E-5
7	0.0	0.0	0.0	2.7062244515497872E-5	2.7062244515497872E-5
8	0.0	0.0	0.0	2.7062244515497872E-5	5.4124489030995744E-5
9	0.0	0.0	0.0	2.7062244515497872E-5	5.4124489030995744E-5
10-11	0.0	2.7062244515497872E-5	0.0	8.118673354649361E-5	5.4124489030995744E-5
12-13	0.0	2.7062244515497872E-5	0.0	8.118673354649361E-5	2.4356020063948087E-4
14-15	0.0	5.4124489030995744E-5	0.0	8.118673354649361E-5	3.2474693418597445E-4
16-17	0.0	8.118673354649361E-5	0.0	8.118673354649361E-5	3.653403009592212E-4
18-19	0.0	8.118673354649361E-5	0.0	8.118673354649361E-5	4.0593366773246804E-4
20-21	0.0	8.118673354649361E-5	0.0	1.3531122257748936E-4	4.0593366773246804E-4
22-23	0.0	1.0824897806199149E-4	0.0	1.894357116084851E-4	4.329959122479659E-4
24-25	0.0	1.0824897806199149E-4	0.0	2.4356020063948084E-4	4.600581567634638E-4
26-27	0.0	1.0824897806199149E-4	0.0	3.382780564437234E-4	4.871204012789617E-4
28-29	0.0	1.0824897806199149E-4	0.0	7.712739686916893E-4	4.871204012789617E-4
30-31	0.0	1.0824897806199149E-4	0.0	0.002300290783817319	4.871204012789617E-4
32-33	0.0	1.0824897806199149E-4	0.0	0.00610253613824477	4.871204012789617E-4
34-35	2.7062244515497872E-5	1.0824897806199149E-4	0.0	0.010906084539745642	5.141826457944595E-4
36-37	2.7062244515497872E-5	1.2178010031974043E-4	0.0	0.018497044126342795	5.277137680522085E-4
38-39	2.7062244515497872E-5	1.894357116084851E-4	0.0	0.030093215901233632	5.683071348254554E-4
40-41	2.7062244515497872E-5	2.1649795612398298E-4	0.0	0.04256891062287815	5.953693793409532E-4
42-43	2.7062244515497872E-5	2.1649795612398298E-4	0.0	0.05476045177710994	6.089005015987021E-4
44-45	2.7062244515497872E-5	2.1649795612398298E-4	0.0	0.06826451179034337	6.359627461141999E-4
46-47	2.7062244515497872E-5	2.1649795612398298E-4	0.0	0.08260750138355724	6.630249906296979E-4
48-49	2.7062244515497872E-5	2.1649795612398298E-4	0.0	0.0958950634406667	7.036183574029446E-4
50-51	2.7062244515497872E-5	2.1649795612398298E-4	0.0	0.10989977497743686	7.306806019184425E-4
52-53	2.7062244515497872E-5	2.1649795612398298E-4	0.0	0.12349855284647454	7.712739686916893E-4
54-55	2.7062244515497872E-5	2.300290783817319E-4	0.0	0.13861281640838008	7.848050909494383E-4
56-57	2.7062244515497872E-5	2.7062244515497873E-4	0.0	0.1561762130989382	7.848050909494383E-4
58-59	2.7062244515497872E-5	2.7062244515497873E-4	0.0	0.17250827766404117	7.848050909494383E-4
60-61	2.7062244515497872E-5	2.976846896704766E-4	0.0	0.18783903918207073	7.848050909494383E-4
62-63	2.7062244515497872E-5	2.976846896704766E-4	0.0	0.20427935272523567	8.659918244959319E-4
64-65	2.7062244515497872E-5	2.976846896704766E-4	0.0	0.22017842137809068	9.201163135269276E-4
66-67	2.7062244515497872E-5	2.976846896704766E-4	0.0	0.23747119562349384	9.201163135269276E-4
68-69	2.7062244515497872E-5	2.976846896704766E-4	0.0	0.2549939989472787	9.201163135269276E-4
70-71	2.7062244515497872E-5	3.518091787014723E-4	0.0	0.272922735938796	9.607096803001744E-4
72-73	2.7062244515497872E-5	4.0593366773246804E-4	0.0	0.29089206629708664	0.0011772076364241573
74-75	2.7062244515497872E-5	4.0593366773246804E-4	0.0	0.3105392558153381	0.0012178010031974043
76-77	2.7062244515497872E-5	4.3299591224796595E-4	0.0	0.33044353665648674	0.0012178010031974043
78-79	2.7062244515497872E-5	4.3299591224796595E-4	1.3531122257748936E-5	0.35120027819987365	0.001244863247712902
80-81	2.7062244515497872E-5	4.4652703450571486E-4	4.0593366773246806E-5	0.37276888707872546	0.0012719254922283999
82-83	2.7062244515497872E-5	4.871204012789617E-4	5.4124489030995744E-5	0.39417512249048425	0.0013531122257748936
84-85	2.7062244515497872E-5	4.871204012789617E-4	6.765561128874468E-5	0.4162037895260995	0.0013531122257748936
86	2.7062244515497872E-5	4.871204012789617E-4	8.118673354649361E-5	0.4334559704047294	0.0013531122257748936
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACATG	11580	0.0	25.361456	1
TACATGG	11845	0.0	24.535166	2
GTACCTG	3675	0.0	22.671658	1
ACATGGG	12370	0.0	22.567446	3
GAGTACA	8840	0.0	21.1415	1
AGTACAT	8375	0.0	20.634377	2
TACCTGG	3820	0.0	20.39954	2
GTGTAGC	1955	0.0	19.072178	1
CATGGGG	9405	0.0	18.667011	4
GTATCAA	16265	0.0	18.33931	1
TCGCGTA	155	0.0012280614	17.732828	9
AGAGTAC	11210	0.0	17.633062	10-11
GTACTTT	6055	0.0	16.730427	14-15
GAGTACT	6210	0.0	16.270536	12-13
TATCAAC	18175	0.0	16.267475	2
TCAACGC	18195	0.0	16.249594	4
GTATAGG	800	0.0	16.111282	1
CAACGCA	18355	0.0	16.107944	5
ATCAACG	18495	0.0	16.060368	3
GTACACG	865	0.0	15.964937	1
>>END_MODULE