FastQCFastQC Report
Mon 15 Aug 2016
SRR2921462_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921462_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3695185
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT86290.23352010792423114No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51790.14015536434576348No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG118500.024.7164361
TACATGG120200.023.8260152
GTACCTG34250.023.7774121
ACATGGG120700.022.9013043
GAGTACA90000.021.9072021
TACCTGG36450.020.9781382
AGTACAT86900.020.8776782
CATGGGG90800.019.6150724
CGTACAC5350.018.6427523
AGAGTAC112800.018.03241210-11
GTATCAA178250.017.7138561
TACACCG4600.017.5428525
GAGTACT60450.016.3310612-13
GTACTTT59450.016.32247214-15
TCAACGC192150.016.1157934
CAACGCA192700.016.0610895
GTGTAGC20550.015.9906581
ATCAACG198350.015.6359933
AACGCAG199000.015.5530456
GTATACG2457.1763716E-515.5508091
Produced by FastQC (version 0.11.5)