FastQCFastQC Report
Mon 15 Aug 2016
SRR2921461_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921461_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4024193
Sequences flagged as poor quality0
Sequence length98
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44770.11125211936902628No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG47150.032.0226171
TACCTGG48150.029.7084662
GTATCAA88850.029.5830761
GTACATG124100.025.557131
TCAACGC104950.024.6824324
TACATGG128400.024.0310062
CAACGCA108950.023.7762365
ATCAACG109250.023.5850493
TATCAAC110300.023.2358322
AACGCAG113950.022.8893496
ACATGGG130750.022.6172563
ACCTGGG66350.021.6284473
GAGTACA97400.021.2675881
AGTACAT90600.021.203512
GGTATCA64300.021.0475251
ACGCAGA131800.019.5396167
CATGGGG99600.019.10344
GTATAGG12100.019.0216981
GTGTAGC23400.018.8851341
CGCAGAG137750.018.5242218
Produced by FastQC (version 0.11.5)