##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921461_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4024193
Sequences flagged as poor quality	0
Sequence length	98
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.108566114994982	31.0	31.0	34.0	26.0	34.0
2	30.192636635469523	31.0	31.0	34.0	26.0	34.0
3	30.328798593904416	31.0	31.0	34.0	26.0	34.0
4	33.82289691374147	37.0	35.0	37.0	30.0	37.0
5	33.800687491877255	37.0	35.0	37.0	30.0	37.0
6	33.90278696871646	37.0	35.0	37.0	30.0	37.0
7	33.78024885983351	37.0	35.0	37.0	29.0	37.0
8	33.754306267119894	37.0	35.0	37.0	29.0	37.0
9	35.17741271355524	39.0	35.0	39.0	29.0	39.0
10-11	35.042962899642234	39.0	35.0	39.0	28.5	39.0
12-13	34.866180498798144	39.0	35.0	39.0	27.0	39.0
14-15	35.83987870363076	39.5	36.0	41.0	27.0	41.0
16-17	35.6823570589184	39.0	36.0	41.0	27.0	41.0
18-19	35.49745849664765	39.0	36.0	40.5	27.0	41.0
20-21	35.37495343786941	39.0	36.0	40.0	26.5	41.0
22-23	35.40672825582669	39.0	36.0	41.0	26.5	41.0
24-25	35.26473531463327	39.0	35.5	41.0	25.5	41.0
26-27	34.98201465486372	39.0	35.0	40.0	24.5	41.0
28-29	34.73751557144501	39.0	34.5	40.0	24.0	41.0
30-31	34.47115123454566	38.0	34.0	40.0	22.5	41.0
32-33	34.18860477119263	38.0	34.0	40.0	20.0	41.0
34-35	33.75654423135272	38.0	33.0	40.0	17.0	41.0
36-37	33.361964995217676	38.0	33.0	40.0	15.0	41.0
38-39	33.116915987876325	38.0	32.5	40.0	11.0	41.0
40-41	32.836636314411365	38.0	32.0	40.0	9.0	41.0
42-43	32.53708271447219	38.0	31.0	40.0	8.5	41.0
44-45	32.20440197575017	37.0	31.0	40.0	8.0	41.0
46-47	31.901442351298755	37.0	30.5	40.0	6.5	41.0
48-49	31.669524423903127	37.0	30.0	40.0	2.0	41.0
50-51	30.945232000552657	36.0	29.5	39.0	2.0	40.0
52-53	31.479721400042195	36.5	30.5	39.0	2.0	40.0
54-55	31.899448783892822	37.0	31.0	40.0	2.0	40.5
56-57	31.737010376987385	37.0	31.0	40.0	2.0	41.0
58-59	31.39469677522922	36.0	31.0	39.0	2.0	41.0
60-61	30.926096114177426	36.0	30.0	39.0	2.0	40.0
62-63	30.42886946525676	35.0	29.0	39.0	2.0	40.0
64-65	29.8723088082505	35.0	28.5	38.0	2.0	40.0
66-67	29.37034269976614	34.5	28.0	38.0	2.0	40.0
68-69	28.837182635127093	34.0	27.5	37.0	2.0	39.0
70-71	28.30018540363248	34.0	26.0	36.5	2.0	39.0
72-73	27.7318784163682	33.5	26.0	36.0	2.0	39.0
74-75	27.117014641196384	33.0	25.0	35.0	2.0	38.0
76-77	26.51461050700103	33.0	23.5	35.0	2.0	37.0
78-79	25.926041941825353	32.0	21.5	35.0	2.0	36.5
80-81	25.327913944485267	32.0	19.0	35.0	2.0	36.0
82-83	24.67739979170979	31.0	16.5	34.0	2.0	35.5
84-85	24.0336494795354	31.0	8.5	34.0	2.0	35.0
86-87	23.38070949877404	30.5	4.0	34.0	2.0	35.0
88-89	22.703049406427574	30.0	2.0	34.0	2.0	35.0
90-91	21.937285811093055	29.0	2.0	34.0	2.0	35.0
92-93	21.095503247483407	29.0	2.0	34.0	2.0	35.0
94-95	20.16412209851764	28.0	2.0	33.0	2.0	34.5
96-97	19.260169927237584	26.5	2.0	33.0	2.0	34.0
98	18.63552742127428	25.0	2.0	33.0	2.0	34.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	147164.0
3	24919.0
4	19118.0
5	15868.0
6	14847.0
7	15837.0
8	17637.0
9	20318.0
10	22865.0
11	24817.0
12	27197.0
13	30505.0
14	26419.0
15	26480.0
16	29603.0
17	33603.0
18	37640.0
19	37141.0
20	38653.0
21	41903.0
22	46049.0
23	50445.0
24	56119.0
25	62894.0
26	70597.0
27	79966.0
28	92428.0
29	106011.0
30	125100.0
31	150698.0
32	184709.0
33	235590.0
34	311439.0
35	425546.0
36	560133.0
37	569260.0
38	234696.0
39	9979.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.844098489287354	16.648221624114083	12.418785433267933	25.088894453330628
2	16.369058865881573	18.379850712130587	38.74978370562484	26.501306716362993
3	20.909755442620014	21.644198351052722	27.099324707356615	30.346721498970652
4	13.779048214809167	14.066413181494172	34.34302247379019	37.81151612990647
5	15.58133648161557	35.51097257254708	32.716532261272945	16.1911586845644
6	35.932099123066365	32.67550670412746	16.32689953593409	15.065494636872092
7	32.17222146951084	29.657140655240095	18.682509238669034	19.488128636580033
8	28.382453895569032	33.752261166396494	18.35333897040438	19.51194596763009
9	26.924559499421047	16.485931593143658	18.5937383889945	37.99577051844079
10-11	27.207203064943652	24.76984962340611	25.6346796932571	22.38826761839314
12-13	27.142997560339445	21.638421006654173	26.405880336221582	24.8127010967848
14-15	23.984474890497722	23.332649442475688	23.958801022928405	28.724074644098184
16-17	24.63530242457358	26.490208268843602	24.471132439857257	24.403356866725563
18-19	24.581423820378483	25.81388017010578	25.85630964741639	23.74838636209935
20-21	25.0250751258979	25.029601456583162	25.96154061374557	23.98378280377337
22-23	24.75536011394183	24.736841581604544	25.82793012556038	24.67986817889325
24-25	24.52504341410485	24.905727329705865	25.987738491609925	24.58149076457936
26-27	24.528851141022404	25.10050325929126	26.003039874634702	24.367605725051632
28-29	24.44630946135181	25.308211729685187	25.888603941165666	24.35687486779734
30-31	24.63182253238767	25.195232300237237	25.783468398766317	24.389476768608777
32-33	24.484264535916694	25.21939863105247	25.759696773809456	24.53664005922138
34-35	24.6717132628004	25.211418567164678	25.843638009652555	24.27323016038237
36-37	24.69295458147967	25.11535767128514	25.781989502301904	24.40969824493329
38-39	24.62099048320176	25.236539297478444	25.805283161825553	24.337187057494248
40-41	24.782367072621607	25.14324367141588	25.736308703667888	24.33808055229462
42-43	24.662494892910054	25.05004042579422	25.718249513772562	24.56921516752317
44-45	24.623421770863697	25.18824852627012	25.692155368508026	24.496174334358155
46-47	24.68123110950605	25.189448146577497	25.681006833669745	24.448313910246707
48-49	24.70633509090264	25.267818301595696	25.522313738358417	24.503532869143246
50-51	24.486894598066293	25.44666863312842	25.58980740080628	24.476629367999006
52-53	24.604533617189016	25.412107534711144	25.529764165569652	24.453594682530188
54-55	24.551575893999487	25.242179003806292	25.669425097556292	24.53682000463792
56-57	24.535038672363342	25.35003703005242	25.6520723715844	24.462851925999843
58-59	24.665374158994926	25.286184897688408	25.621719123440563	24.426721819876107
60-61	24.688204871784052	25.250953399381743	25.692405598538	24.36843613029621
62-63	24.52421543538075	25.39401523979382	25.643557184197675	24.43821214062775
64-65	24.55829457321733	25.26985510208212	25.667358131979352	24.5044921927212
66-67	24.580792263886707	25.329368581935807	25.55621556998757	24.533623584189908
68-69	24.47364828738753	25.323931563193113	25.657070854608317	24.545349294811036
70-71	24.53014796265026	25.146585147461458	25.66675317409226	24.65651371579602
72-73	24.615276702852793	25.209371673426993	25.599939192632426	24.57541243108779
74-75	24.44140877061824	25.445976382391077	25.574869811895955	24.537745035094726
76-77	24.664898863424906	25.176219747419715	25.50792310243104	24.65095828672434
78-79	24.535323454361368	25.190055667962515	25.649818352494375	24.624802525181742
80-81	24.556772371408204	25.333384748087674	25.54520854423366	24.564634336270462
82-83	24.704447553201685	25.22755254265721	25.514729817708332	24.55327008643277
84-85	24.601455848762225	25.295091495270828	25.44533554671191	24.658117109255038
86-87	24.615727858845847	25.239432711088416	25.60405497996206	24.540784450103676
88-89	24.569074404871856	25.07299000406388	25.567382454640857	24.79055313642341
90-91	24.558005003165974	25.09882912538439	25.594902790676432	24.748263080773203
92-93	24.693463231872563	25.194059777337113	25.603486755517686	24.50899023527264
94-95	24.744695829583645	25.076785625366522	25.529818007054384	24.648700537995452
96-97	24.646911832534325	25.043153279249886	25.59999031609465	24.709944572121135
98	24.723641944081248	25.097027614467127	25.382438918961007	24.796891522490615
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	547.0
1	393.0
2	383.0
3	555.0
4	464.0
5	416.5
6	700.0
7	1341.0
8	1989.0
9	2037.0
10	1669.5
11	1454.0
12	1604.0
13	1879.0
14	2126.0
15	2444.5
16	2740.5
17	2893.0
18	2978.5
19	2887.0
20	2755.5
21	2690.0
22	2649.0
23	2824.0
24	3149.5
25	3800.0
26	4837.5
27	6447.0
28	8670.0
29	11546.5
30	15142.5
31	19890.0
32	26015.0
33	32615.0
34	40227.0
35	49072.5
36	58707.0
37	68633.0
38	78515.0
39	88751.0
40	99743.5
41	111347.5
42	122775.0
43	134321.0
44	145789.5
45	156210.0
46	167858.0
47	179598.0
48	184612.5
49	184574.5
50	184491.0
51	183900.5
52	183634.0
53	182956.5
54	179862.0
55	172636.5
56	161610.5
57	149725.5
58	137209.0
59	122366.0
60	106270.5
61	89769.5
62	72949.0
63	56744.0
64	42715.5
65	32619.0
66	25049.0
67	19269.5
68	14849.0
69	11418.5
70	8702.0
71	6660.0
72	5199.5
73	3859.0
74	2923.0
75	2238.0
76	1528.0
77	966.0
78	591.5
79	335.0
80	200.5
81	125.5
82	78.0
83	49.5
84	39.5
85	29.5
86	21.5
87	18.5
88	17.5
89	15.0
90	8.5
91	5.5
92	7.0
93	6.5
94	5.5
95	5.0
96	3.5
97	2.5
98	2.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.462204471803415
2	0.0471398861833913
3	0.055489386319194936
4	0.052929866932326555
5	0.06284489834359336
6	0.06376433734664316
7	0.042194795329150464
8	0.020550704203302378
9	0.013220041881689074
10-11	0.015605613349061537
12-13	0.011865733079899498
14-15	0.01606533285058644
16-17	0.015394390875387937
18-19	0.05529058869691389
20-21	0.08140762632408535
22-23	0.09681444205086585
24-25	0.0682497086993591
26-27	0.08209099315067642
28-29	0.08392987115677603
30-31	0.05599880522629009
32-33	0.03283888223054908
34-35	0.3880653835439801
36-37	0.6077988804214907
38-39	0.5586088937583262
40-41	0.5534277307276266
42-43	0.5263042801376574
44-45	0.5113074845068315
46-47	0.5018397477456971
48-49	0.4919992654427856
50-51	0.5065860409776569
52-53	0.5856702200913326
54-55	0.5566581920896935
56-57	0.5140906512187661
58-59	0.4928317304860875
60-61	0.5059896481108138
62-63	0.5183151006922382
64-65	0.5363062855086722
66-67	0.561528733835579
68-69	0.5159419540762582
70-71	0.4660934502892878
72-73	0.4497671955594575
74-75	0.43224815509594094
76-77	0.444871804110787
78-79	0.4390818233618517
80-81	0.43638563060966507
82-83	0.4548738094818017
84-85	0.4235880336753232
86-87	0.42665697196928676
88-89	0.45139485109188354
90-91	0.4356152898233261
92-93	0.43172631133745326
94-95	0.4409952504763066
96-97	0.43598803536510305
98	0.43099324510529186
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	4024193.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.01855220741684
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.2009371084571	43.63049495673688
2	13.801602993858255	16.014980476904114
3	4.775749025396554	8.312461324784701
4	2.0855780696021555	4.840088804554251
5	1.1308650139620768	3.2805575426049964
6	0.7196699103579502	2.5052523759725838
7	0.43602030571558503	1.7708086809457464
8	0.328436484108215	1.5244327440042325
9	0.23039311425549597	1.203036743489566
>10	1.2107482627897437	12.312724670305313
>50	0.06008473067636928	2.352535780361031
>100	0.019312860519426118	1.7922936871882542
>500	4.300859591425846E-4	0.16900641987167087
>1k	1.7203434214666514E-4	0.2913257922768368
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4477	0.11125211936902628	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.4849702785129837E-5
2	0.0	0.0	0.0	2.4849702785129837E-5	2.4849702785129837E-5
3	0.0	0.0	0.0	4.9699405570259674E-5	2.4849702785129837E-5
4	0.0	0.0	0.0	4.9699405570259674E-5	2.4849702785129837E-5
5	0.0	0.0	0.0	4.9699405570259674E-5	2.4849702785129837E-5
6	0.0	0.0	0.0	4.9699405570259674E-5	2.4849702785129837E-5
7	0.0	0.0	0.0	9.939881114051935E-5	2.4849702785129837E-5
8	0.0	0.0	0.0	1.242485139256492E-4	2.4849702785129837E-5
9	0.0	0.0	0.0	1.4909821671077903E-4	2.4849702785129837E-5
10-11	0.0	0.0	0.0	1.7394791949590886E-4	2.4849702785129837E-5
12-13	0.0	2.4849702785129837E-5	0.0	1.7394791949590886E-4	1.3667336531821413E-4
14-15	0.0	2.4849702785129837E-5	0.0	2.2364732506616856E-4	1.7394791949590886E-4
16-17	0.0	2.4849702785129837E-5	0.0	2.3607217645873346E-4	1.987976222810387E-4
18-19	0.0	2.4849702785129837E-5	0.0	2.7334673063642825E-4	2.2364732506616856E-4
20-21	0.0	2.4849702785129837E-5	0.0	3.230461362066879E-4	3.230461362066879E-4
22-23	0.0	2.4849702785129837E-5	0.0	3.3547098759925285E-4	3.478958389918177E-4
24-25	0.0	2.4849702785129837E-5	0.0	3.478958389918177E-4	3.727455417769476E-4
26-27	0.0	2.4849702785129837E-5	0.0	4.2244494734720726E-4	3.727455417769476E-4
28-29	0.0	2.4849702785129837E-5	0.0	8.697395974795444E-4	3.727455417769476E-4
30-31	0.0	2.4849702785129837E-5	0.0	0.0028452909688973663	3.727455417769476E-4
32-33	0.0	2.4849702785129837E-5	0.0	0.0070945901451545685	3.727455417769476E-4
34-35	0.0	2.4849702785129837E-5	0.0	0.012263328324461576	3.975952445620774E-4
36-37	0.0	3.727455417769476E-5	0.0	0.020115834404562605	3.975952445620774E-4
38-39	0.0	4.9699405570259674E-5	0.0	0.03150942313154463	4.2244494734720726E-4
40-41	0.0	4.9699405570259674E-5	0.0	0.043188783440555656	4.472946501323371E-4
42-43	0.0	4.9699405570259674E-5	0.0	0.05486814374956668	4.472946501323371E-4
44-45	0.0	6.21242569628246E-5	0.0	0.0673302696963093	4.721443529174669E-4
46-47	0.0	7.454910835538951E-5	0.0	0.08004089267090321	5.342686098802915E-4
48-49	0.0	7.454910835538951E-5	0.0	0.09342245762069562	5.963928668431161E-4
50-51	0.0	7.454910835538951E-5	0.0	0.10723889236922782	6.460922724133758E-4
52-53	0.0	8.697395974795443E-5	0.0	0.11945252128811913	6.833668265910706E-4
54-55	0.0	9.939881114051935E-5	0.0	0.13360442702425057	7.206413807687653E-4
56-57	0.0	9.939881114051935E-5	0.0	0.14886214453432028	7.454910835538952E-4
58-59	0.0	9.939881114051935E-5	0.0	0.16332467155526587	7.70340786339025E-4
60-61	0.0	1.242485139256492E-4	0.0	0.17856996421394303	8.200401919092846E-4
62-63	0.0	1.242485139256492E-4	0.0	0.1948713692409882	8.821644488721094E-4
64-65	0.0	1.242485139256492E-4	0.0	0.21142127129588467	8.945893002646742E-4
66-67	0.0	1.242485139256492E-4	0.0	0.22821967037863244	9.194390030498041E-4
68-69	1.2424851392564919E-5	1.242485139256492E-4	0.0	0.24645935222291773	9.442887058349338E-4
70-71	2.4849702785129837E-5	1.242485139256492E-4	0.0	0.2646244849588476	9.442887058349338E-4
72-73	3.727455417769476E-5	1.242485139256492E-4	0.0	0.28256597036971143	0.001043687516975453
74-75	4.9699405570259674E-5	1.242485139256492E-4	0.0	0.29936436945245914	0.0011182366253308428
76-77	4.9699405570259674E-5	1.242485139256492E-4	0.0	0.3177904240676329	0.0011182366253308428
78-79	4.9699405570259674E-5	1.242485139256492E-4	0.0	0.3367258975899019	0.001217635436471362
80-81	4.9699405570259674E-5	1.3667336531821413E-4	0.0	0.3561583651678734	0.001254909990649057
82-83	4.9699405570259674E-5	1.4909821671077903E-4	0.0	0.37623692501825834	0.0013543088017895762
84-85	4.9699405570259674E-5	1.4909821671077903E-4	0.0	0.3963030600172507	0.001379158504574706
86	4.9699405570259674E-5	1.7394791949590886E-4	0.0	0.41138682960782447	0.0014164330587524009
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCTG	4715	0.0	32.022617	1
TACCTGG	4815	0.0	29.708466	2
GTATCAA	8885	0.0	29.583076	1
GTACATG	12410	0.0	25.55713	1
TCAACGC	10495	0.0	24.682432	4
TACATGG	12840	0.0	24.031006	2
CAACGCA	10895	0.0	23.776236	5
ATCAACG	10925	0.0	23.585049	3
TATCAAC	11030	0.0	23.235832	2
AACGCAG	11395	0.0	22.889349	6
ACATGGG	13075	0.0	22.617256	3
ACCTGGG	6635	0.0	21.628447	3
GAGTACA	9740	0.0	21.267588	1
AGTACAT	9060	0.0	21.20351	2
GGTATCA	6430	0.0	21.047525	1
ACGCAGA	13180	0.0	19.539616	7
CATGGGG	9960	0.0	19.1034	4
GTATAGG	1210	0.0	19.021698	1
GTGTAGC	2340	0.0	18.885134	1
CGCAGAG	13775	0.0	18.524221	8
>>END_MODULE