FastQCFastQC Report
Mon 15 Aug 2016
SRR2921461_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921461_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4024193
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43760.10874229938772817No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG45150.034.3842351
TACCTGG47450.031.7244432
GTATCAA90600.028.3834421
GTACATG121500.026.1426161
TACATGG127850.024.4397162
TCAACGC104800.024.1535054
CAACGCA107450.023.6803935
ACATGGG127850.023.0665953
ATCAACG110100.023.0327913
TATCAAC110950.023.0263962
ACCTGGG62400.022.679023
AACGCAG114800.022.2064486
GTATAGG9250.022.1373791
GGTATCA66000.021.8624171
GAGTACA98550.021.8414421
AGTACAT90350.020.6554852
CATGGGG97300.020.2558044
TATCACG3655.456968E-1219.515042
ACGCAGA134300.018.7707427
CGCAGAG142500.017.7574548
Produced by FastQC (version 0.11.5)