##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921461_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4024193
Sequences flagged as poor quality	0
Sequence length	101
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.868173320712998	33.0	31.0	34.0	30.0	34.0
2	31.979536766750503	34.0	31.0	34.0	30.0	34.0
3	32.10713750558187	34.0	31.0	34.0	30.0	34.0
4	35.656385267804005	37.0	35.0	37.0	35.0	37.0
5	35.45156606554407	37.0	35.0	37.0	33.0	37.0
6	35.490278672021944	37.0	35.0	37.0	33.0	37.0
7	35.419382469975965	37.0	35.0	37.0	33.0	37.0
8	35.37137483217132	37.0	35.0	37.0	33.0	37.0
9	36.982720510671335	39.0	37.0	39.0	33.0	39.0
10-11	36.88938477851336	39.0	37.0	39.0	33.0	39.0
12-13	36.76267067707737	39.0	37.0	39.0	33.0	39.0
14-15	38.044874711526006	40.0	38.0	41.0	33.0	41.0
16-17	37.94503581214917	40.0	38.0	41.0	33.0	41.0
18-19	37.83243808137433	40.0	38.0	41.0	33.0	41.0
20-21	37.71313465830292	40.0	38.0	41.0	32.0	41.0
22-23	37.67537864113376	40.0	38.0	41.0	32.5	41.0
24-25	37.729967474224026	40.0	38.0	41.0	32.5	41.0
26-27	37.616688985841385	40.0	38.0	41.0	32.0	41.0
28-29	37.47721319529158	40.0	38.0	41.0	32.0	41.0
30-31	37.33570271107773	40.0	37.0	41.0	32.0	41.0
32-33	37.167616463723284	40.0	37.0	41.0	31.0	41.0
34-35	37.015671465061445	40.0	37.0	41.0	31.0	41.0
36-37	36.911025514929335	40.0	37.0	41.0	31.0	41.0
38-39	36.874777253476665	40.0	37.0	41.0	31.0	41.0
40-41	36.70895928699245	40.0	36.0	41.0	30.0	41.0
42-43	36.46085823915503	39.0	36.0	41.0	30.0	41.0
44-45	36.243527460039815	39.0	35.5	41.0	30.0	41.0
46-47	35.96110338147301	39.0	35.0	40.0	29.0	41.0
48-49	35.61793942288553	39.0	35.0	40.0	28.0	41.0
50-51	35.33109333970811	38.0	35.0	40.0	27.5	41.0
52-53	35.00706228553153	38.0	34.0	40.0	26.5	41.0
54-55	34.66523635919052	38.0	34.0	40.0	26.0	41.0
56-57	34.268927956487175	37.5	33.5	40.0	25.5	41.0
58-59	33.868779280715415	37.0	33.0	40.0	24.0	41.0
60-61	33.42636809417441	36.5	32.5	39.0	23.0	40.5
62-63	32.967567410409984	36.0	32.0	39.0	22.0	40.0
64-65	32.42799115251182	35.0	31.0	38.5	20.5	40.0
66-67	31.882748416887562	35.0	31.0	38.0	18.5	40.0
68-69	31.3583918564542	35.0	30.5	37.5	17.5	39.5
70-71	30.787173726508644	34.0	30.0	37.0	12.5	39.0
72-73	30.220105869673745	34.0	29.0	36.0	8.0	39.0
74-75	29.678492805886794	34.0	29.0	35.5	7.0	38.0
76-77	28.44547962784091	32.0	27.0	34.5	7.0	36.0
78-79	29.371625938417964	34.0	29.0	35.0	7.0	37.0
80-81	29.388865916719205	34.0	29.0	35.0	2.0	36.5
82-83	29.027690769304552	34.0	29.0	35.0	2.0	36.0
84-85	28.504837988635238	33.5	29.0	35.0	2.0	35.5
86-87	27.956939441025817	33.0	27.0	35.0	2.0	35.0
88-89	27.385289174748827	33.0	26.0	35.0	2.0	35.0
90-91	26.87020068371472	32.5	25.0	34.5	2.0	35.0
92-93	26.397292575182156	32.0	25.0	34.0	2.0	35.0
94-95	25.851528989787518	32.0	24.0	34.0	2.0	35.0
96-97	25.02824901787762	32.0	19.5	34.0	2.0	35.0
98-99	23.96359220345545	31.0	6.5	34.0	2.0	35.0
100-101	20.407738271996397	27.0	2.0	32.0	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	28395.0
3	14630.0
4	8637.0
5	6327.0
6	5638.0
7	5794.0
8	6877.0
9	8198.0
10	9718.0
11	11513.0
12	13591.0
13	15818.0
14	17989.0
15	20453.0
16	22951.0
17	26709.0
18	27390.0
19	25130.0
20	25636.0
21	27258.0
22	29071.0
23	32050.0
24	36410.0
25	42410.0
26	49923.0
27	60209.0
28	73346.0
29	88613.0
30	108001.0
31	133885.0
32	170672.0
33	225858.0
34	314859.0
35	462716.0
36	694246.0
37	820763.0
38	342315.0
39	10194.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.993391924438114	17.134408406489126	12.820887999724809	25.051311669347953
2	16.47050725449798	18.646272681255596	38.388790000877194	26.49443006336923
3	21.00405125526288	21.77839221180843	27.222039703395033	29.99551682953366
4	14.065257935457145	14.365487900421614	34.245609742816676	37.32364442130457
5	15.660165619163397	35.44110440607409	32.740027016737855	16.158702958024655
6	36.072887913274904	32.77526732339921	16.107933430922362	15.043911332403528
7	32.226269376334024	29.6854899536383	18.627068937261875	19.46117173276581
8	28.40369689151264	33.77376166223801	18.265603257292366	19.556938188956988
9	26.812472127340836	16.519417776370553	18.575894592660596	38.09221550362802
10-11	27.176949374971006	24.845525874905654	25.618670689881824	22.35885406024152
12-13	27.148434779494142	21.620752404243166	26.453191629074972	24.77762118718772
14-15	23.878959044099155	23.380591528734428	23.983978637520277	28.756470789646137
16-17	24.597705235366067	26.507809117881713	24.57773141387436	24.316754232877855
18-19	24.511321670988227	25.87216200020807	25.93454607105271	23.681970257750994
20-21	24.9718964237337	24.995211983302777	26.0423366316896	23.990554961273926
22-23	24.654130969139313	24.741887066851874	25.97362966553864	24.630352298470175
24-25	24.477631084888934	24.926882458961767	26.109950436306423	24.48553601984288
26-27	24.47903973725153	25.120016066351248	26.109622772561586	24.29132142383564
28-29	24.389563706167362	25.276960371880396	26.043285132414784	24.29019078953746
30-31	24.558278443655055	25.17351582911423	25.90594838815012	24.3622573390806
32-33	24.359681820616103	25.204534392953796	25.86679304311565	24.568990743314448
34-35	24.560961626064184	25.198684046700535	26.00636802903487	24.233986298200406
36-37	24.527061375435334	25.166986579563872	25.963767891729894	24.342184153270896
38-39	24.47466250117279	25.25456175636707	25.98418181038677	24.286593932073373
40-41	24.657472109432952	25.15839689652314	25.93386843354874	24.250262560495166
42-43	24.556994928742203	25.128943445299278	25.827803872267026	24.486257753691493
44-45	24.48431807260536	25.18729052644895	25.86701282188453	24.46137857906116
46-47	24.556818082960604	25.214472930365737	25.829686806761227	24.399022179912432
48-49	24.569430604186287	25.33458496206596	25.684266504973795	24.41171792877396
50-51	24.414787780074747	25.475076626464826	25.67569028432218	24.434445309138255
52-53	24.55816795668693	25.414248215189644	25.602004623646003	24.425579204477426
54-55	24.525597853996675	25.249447787341	25.698387329047673	24.52656702961465
56-57	24.490817835062227	25.418117215711234	25.681496084418416	24.409568864808126
58-59	24.63328577461791	25.30083072232548	25.658285224812154	24.407598278244453
60-61	24.63792102553092	25.346025232704704	25.677699016695893	24.33835472506849
62-63	24.43790802159505	25.423441526043483	25.705017201603287	24.433633250758184
64-65	24.480108431686944	25.324062430726684	25.70754459089106	24.488284546695308
66-67	24.5660200953071	25.356401036572507	25.60481748426205	24.472761383858348
68-69	24.45516080802967	25.39289166024124	25.650687767092357	24.50125976463673
70-71	24.467851517721506	25.224428932236087	25.68783182198032	24.619887728062082
72-73	24.63149704848391	25.238902218717318	25.594115735225486	24.53548499757329
74-75	24.40421812412043	25.470844481317446	25.54346616770438	24.581471226857744
76-77	24.568517974824626	25.288373300926594	25.5585527027411	24.58455602150768
78-79	24.472115674062227	25.256756923102174	25.74031128765591	24.53081611517969
80-81	24.460644646169463	25.45037632449368	25.60561421755379	24.483364811783066
82-83	24.615647138203474	25.35941558405242	25.597192806978015	24.42774447076609
84-85	24.575521658899472	25.406589413507874	25.478443376231546	24.539445551361105
86-87	24.4834335635238	25.41438326556989	25.633689285787586	24.468493885118725
88-89	24.48717851044939	25.23612650248269	25.630689594922135	24.64600539214578
90-91	24.474556348910433	25.328112642707772	25.63552677233701	24.561804236044786
92-93	24.576498731323507	25.428349238818964	25.652300598015763	24.342851431841765
94-95	24.57647610825233	25.31754351172432	25.56362575418914	24.54235462583421
96-97	24.460137462254046	25.36006367886607	25.60305928938819	24.5767395694917
98-99	24.506937978509395	25.314541118707794	25.553347697184115	24.625173205598696
100-101	24.98391167831359	24.759923968933975	25.67327094498185	24.58289340777058
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	199.0
1	167.0
2	261.5
3	401.5
4	316.5
5	253.5
6	527.0
7	1185.0
8	1751.5
9	1666.0
10	1164.5
11	814.0
12	776.0
13	817.0
14	867.0
15	957.0
16	1030.5
17	1128.5
18	1246.0
19	1319.0
20	1436.0
21	1624.0
22	1820.5
23	2072.5
24	2466.5
25	3128.5
26	4051.0
27	5227.0
28	6929.0
29	9432.5
30	12751.5
31	16722.0
32	21683.0
33	27970.0
34	34855.5
35	42279.5
36	51684.5
37	61737.0
38	70783.0
39	80522.5
40	91494.5
41	102490.5
42	112927.0
43	124106.5
44	135916.0
45	146946.0
46	157189.5
47	167693.5
48	179619.0
49	184912.5
50	183084.5
51	182247.5
52	181535.5
53	180467.0
54	178779.0
55	172082.5
56	160974.0
57	148316.0
58	135269.5
59	120882.5
60	104712.5
61	89171.0
62	73381.0
63	56862.0
64	42254.0
65	31822.5
66	24440.5
67	19149.5
68	14945.0
69	11356.0
70	8781.5
71	6784.0
72	5062.0
73	3870.5
74	2909.0
75	2152.0
76	1530.0
77	896.5
78	528.5
79	320.0
80	190.5
81	107.0
82	51.5
83	23.0
84	12.0
85	9.0
86	6.5
87	3.0
88	1.5
89	1.0
90	0.5
91	1.0
92	1.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.30900605413308957
2	0.0
3	0.006336674210208108
4	0.056557923538955515
5	5.218437584877266E-4
6	0.06376433734664316
7	0.07420121251639769
8	0.10354871150563603
9	0.09134750743813728
10-11	0.09590742789920861
12-13	0.0944537202862785
14-15	0.06367736338689521
16-17	0.03547295072577285
18-19	0.03805731981542635
20-21	0.02804288959301902
22-23	0.0417599255304107
24-25	0.03452866201993791
26-27	0.021805614193951436
28-29	0.023955113484865165
30-31	0.0033919844301702226
32-33	0.005876954708683207
34-35	0.008498598352514404
36-37	0.010971143779634825
38-39	0.01641322868957826
40-41	0.02073707697419085
42-43	0.003528657795488437
44-45	0.014027657222205793
46-47	0.023806015268154384
48-49	0.0025595193868683734
50-51	0.0071939889562950875
52-53	0.0023855714673724645
54-55	0.0038392790803025603
56-57	0.003528657795488437
58-59	0.0011058117739382778
60-61	0.005193587882092136
62-63	0.01454950098069352
64-65	0.006883367671480964
66-67	0.037585175462508884
68-69	0.05988778371216291
70-71	0.011940282188254887
72-73	0.03132305036065616
74-75	0.049165136960379385
76-77	0.29469262532885476
78-79	0.07277235460625274
80-81	0.033174353218148336
82-83	0.027322248212250256
84-85	0.018749100751380463
86-87	0.11668177942757715
88-89	0.5163146996180352
90-91	0.12883328408950565
92-93	0.046990787966680526
94-95	0.008324650433018495
96-97	0.006262125101852719
98-99	0.010797195860138914
100-101	0.007740682417567944
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4024193.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.513754760176845
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.12883325853291	38.06370939374641
2	15.82910528199637	16.941497162675475
3	5.57781077762133	8.954687941568976
4	2.5365106714576413	5.42952840075823
5	1.3448081341503524	3.5982866345206483
6	0.8325689668016713	2.673233490621507
7	0.5448741678901385	2.0410783816939815
8	0.37387561980961015	1.6005990583440477
9	0.275166909863153	1.3252693079279212
>10	1.4498987978413127	13.76506172742443
>50	0.0795547991993151	2.8828125535446802
>100	0.02629545578795502	2.237471296105836
>500	5.112499815808792E-4	0.19467104810349095
>1k	1.859090666249134E-4	0.2920936029643721
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4376	0.10874229938772817	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.4849702785129837E-5
2	0.0	0.0	0.0	2.4849702785129837E-5	2.4849702785129837E-5
3	0.0	0.0	0.0	2.4849702785129837E-5	2.4849702785129837E-5
4	0.0	0.0	0.0	2.4849702785129837E-5	2.4849702785129837E-5
5	0.0	2.4849702785129837E-5	0.0	2.4849702785129837E-5	4.9699405570259674E-5
6	0.0	2.4849702785129837E-5	0.0	2.4849702785129837E-5	4.9699405570259674E-5
7	0.0	2.4849702785129837E-5	0.0	2.4849702785129837E-5	7.454910835538951E-5
8	0.0	2.4849702785129837E-5	0.0	4.9699405570259674E-5	7.454910835538951E-5
9	0.0	2.4849702785129837E-5	0.0	1.4909821671077903E-4	9.939881114051935E-5
10-11	0.0	2.4849702785129837E-5	0.0	1.4909821671077903E-4	1.242485139256492E-4
12-13	0.0	2.4849702785129837E-5	0.0	1.4909821671077903E-4	2.1122247367360363E-4
14-15	0.0	2.4849702785129837E-5	0.0	1.6152306810334393E-4	2.9819643342155806E-4
16-17	0.0	2.4849702785129837E-5	0.0	1.7394791949590886E-4	3.975952445620774E-4
18-19	0.0	2.4849702785129837E-5	0.0	2.1122247367360363E-4	4.721443529174669E-4
20-21	0.0	2.4849702785129837E-5	0.0	2.484970278512984E-4	4.969940557025968E-4
22-23	0.0	2.4849702785129837E-5	0.0	2.6092187924386327E-4	6.08817718235681E-4
24-25	0.0	2.4849702785129837E-5	0.0	2.733467306364282E-4	6.460922724133758E-4
26-27	0.0	2.4849702785129837E-5	0.0	3.2304613620668787E-4	6.460922724133758E-4
28-29	0.0	2.4849702785129837E-5	0.0	7.330662321613302E-4	6.460922724133758E-4
30-31	0.0	2.4849702785129837E-5	0.0	0.002658918198008893	6.460922724133758E-4
32-33	0.0	2.4849702785129837E-5	0.0	0.006858517968695835	6.833668265910705E-4
34-35	0.0	2.4849702785129837E-5	0.0	0.011915432485469758	7.206413807687653E-4
36-37	0.0	2.4849702785129837E-5	0.0	0.019631265200252573	7.206413807687653E-4
38-39	0.0	2.4849702785129837E-5	0.0	0.030975154521664345	7.454910835538952E-4
40-41	0.0	2.4849702785129837E-5	0.0	0.04260481542510511	7.454910835538952E-4
42-43	0.0	2.4849702785129837E-5	0.0	0.05424690117993844	7.579159349464602E-4
44-45	0.0	2.4849702785129837E-5	0.0	0.06665932772111079	7.827656377315899E-4
46-47	0.0	2.4849702785129837E-5	0.0	0.07923327733038649	7.951904891241548E-4
48-49	0.0	2.4849702785129837E-5	0.0	0.09247816891486069	8.076153405167197E-4
50-51	0.0	2.4849702785129837E-5	0.0	0.10617035514946724	8.448898946944145E-4
52-53	0.0	2.4849702785129837E-5	0.0	0.11817276159468496	9.194390030498041E-4
54-55	0.0	4.9699405570259674E-5	1.2424851392564919E-5	0.13212586970853535	0.0010312626655828883
56-57	0.0	4.9699405570259674E-5	2.4849702785129837E-5	0.14704811623100583	0.0011058117739382778
58-59	0.0	7.454910835538951E-5	2.4849702785129837E-5	0.16147336869777368	0.0011182366253308428
60-61	0.0	7.454910835538951E-5	2.4849702785129837E-5	0.1765944128425252	0.0011182366253308428
62-63	0.0	7.454910835538951E-5	2.4849702785129837E-5	0.19233669955690497	0.0012673348420416217
64-65	0.0	7.454910835538951E-5	2.4849702785129837E-5	0.20843930696166907	0.0012921845448267515
66-67	0.0	7.454910835538951E-5	2.4849702785129837E-5	0.22465373802896632	0.0013294590990044464
68-69	0.0	7.454910835538951E-5	2.4849702785129837E-5	0.24252067433147467	0.0013418839503970112
70-71	0.0	7.454910835538951E-5	2.4849702785129837E-5	0.2604248851881607	0.001391583355967271
72-73	0.0	7.454910835538951E-5	2.4849702785129837E-5	0.277894226246107	0.0014909821671077904
74-75	0.0	8.697395974795443E-5	2.4849702785129837E-5	0.2943447294898629	0.0015158318698929203
76-77	0.0	9.939881114051935E-5	2.4849702785129837E-5	0.31239803856325976	0.00154068157267805
78-79	0.0	9.939881114051935E-5	2.4849702785129837E-5	0.33103531565210714	0.0015655312754631798
80-81	0.0	1.1182366253308427E-4	2.4849702785129837E-5	0.3502565607564051	0.0015903809782483098
82-83	0.0	1.242485139256492E-4	2.4849702785129837E-5	0.3698008519969097	0.001689779789388829
84-85	0.0	1.242485139256492E-4	2.4849702785129837E-5	0.3896184899680507	0.001689779789388829
86-87	0.0	1.242485139256492E-4	2.4849702785129837E-5	0.4094237030877992	0.0017022046407813938
88-89	0.0	1.4909821671077903E-4	2.4849702785129837E-5	0.43132871609289114	0.0017146294921739588
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCTG	4515	0.0	34.384235	1
TACCTGG	4745	0.0	31.724443	2
GTATCAA	9060	0.0	28.383442	1
GTACATG	12150	0.0	26.142616	1
TACATGG	12785	0.0	24.439716	2
TCAACGC	10480	0.0	24.153505	4
CAACGCA	10745	0.0	23.680393	5
ACATGGG	12785	0.0	23.066595	3
ATCAACG	11010	0.0	23.032791	3
TATCAAC	11095	0.0	23.026396	2
ACCTGGG	6240	0.0	22.67902	3
AACGCAG	11480	0.0	22.206448	6
GTATAGG	925	0.0	22.137379	1
GGTATCA	6600	0.0	21.862417	1
GAGTACA	9855	0.0	21.841442	1
AGTACAT	9035	0.0	20.655485	2
CATGGGG	9730	0.0	20.255804	4
TATCACG	365	5.456968E-12	19.51504	2
ACGCAGA	13430	0.0	18.770742	7
CGCAGAG	14250	0.0	17.757454	8
>>END_MODULE