FastQCFastQC Report
Mon 15 Aug 2016
SRR2921460_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921460_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6098260
Sequences flagged as poor quality0
Sequence length98
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT104730.171737512011623No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG65500.025.7912351
GTACATG186250.025.6783221
TACATGG190300.024.2134532
TACCTGG70150.023.5944042
ACATGGG197050.022.8953933
GAGTACA144550.020.634991
CATGGGG151800.020.4477884
AGTACAT135850.019.8785692
GTATCAA227600.018.869341
ACCTGGG89150.017.3315733
AGAGTAC167600.017.2343510-11
TCAACGC255150.016.6037394
TATCAAC257700.016.439442
CAACGCA259300.016.3026375
ATCAACG261950.016.1902223
GTATAGG13450.016.085071
AACGCAG265850.016.0001166
GTGTAGC33050.015.877451
GTATATA14450.015.6090191
TATACCG4803.8198777E-1115.2829875
Produced by FastQC (version 0.11.5)