##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921460_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6098260
Sequences flagged as poor quality	0
Sequence length	98
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.61331494557464	31.0	31.0	34.0	28.0	34.0
2	30.72570798883616	31.0	31.0	34.0	27.0	34.0
3	30.857987360329012	31.0	31.0	34.0	27.0	34.0
4	34.38443359253295	37.0	35.0	37.0	31.0	37.0
5	34.37459750814167	37.0	35.0	37.0	31.0	37.0
6	34.46987878509608	37.0	35.0	37.0	32.0	37.0
7	34.36041264229469	37.0	35.0	37.0	31.0	37.0
8	34.346560986248534	37.0	35.0	37.0	31.0	37.0
9	35.82574258886961	39.0	37.0	39.0	31.0	39.0
10-11	35.7084386365947	39.0	37.0	39.0	30.0	39.0
12-13	35.5564077294179	39.0	36.0	39.0	30.0	39.0
14-15	36.597486578138685	40.0	37.0	41.0	30.0	41.0
16-17	36.46436877076412	40.0	36.5	41.0	30.0	41.0
18-19	36.288895521017466	40.0	36.0	41.0	29.5	41.0
20-21	36.17347013738345	39.0	36.0	41.0	29.0	41.0
22-23	36.208157654806456	39.5	36.5	41.0	29.0	41.0
24-25	36.07711084801238	39.0	36.0	41.0	28.5	41.0
26-27	35.818323423402745	39.0	36.0	41.0	27.0	41.0
28-29	35.59201534536081	39.0	36.0	40.0	27.0	41.0
30-31	35.348827534411456	39.0	35.0	40.0	26.5	41.0
32-33	35.08488413088324	38.5	35.0	40.0	25.0	41.0
34-35	34.66808737246362	38.0	34.5	40.0	24.5	41.0
36-37	34.286422274550446	38.0	34.0	40.0	22.5	41.0
38-39	34.05621046003286	38.0	33.5	40.0	21.0	41.0
40-41	33.795206501526664	38.0	33.0	40.0	19.0	41.0
42-43	33.5085197252987	38.0	33.0	40.0	17.5	41.0
44-45	33.19376658259897	38.0	32.5	40.0	15.5	41.0
46-47	32.906785952058456	38.0	32.0	40.0	14.5	41.0
48-49	32.7004541459367	37.0	32.0	40.0	10.5	41.0
50-51	31.94614562186591	36.0	30.5	39.0	10.5	40.0
52-53	32.469539999934405	37.0	31.5	39.0	12.0	40.0
54-55	32.916444936752455	37.0	33.0	40.0	10.0	41.0
56-57	32.75993603093342	37.0	32.0	40.0	8.0	41.0
58-59	32.432215418824384	37.0	32.0	40.0	7.0	41.0
60-61	31.968737721907562	36.0	31.0	39.0	3.5	40.5
62-63	31.489221187683043	35.5	31.0	39.0	2.0	40.0
64-65	30.942708739870064	35.0	30.0	38.5	2.0	40.0
66-67	30.450698969870096	35.0	29.5	38.0	2.0	40.0
68-69	29.927839744451695	34.5	29.0	37.0	2.0	39.5
70-71	29.398917724072113	34.0	28.5	36.5	2.0	39.0
72-73	28.842786302978226	34.0	28.0	36.0	2.0	39.0
74-75	28.24396663966443	34.0	26.5	35.5	2.0	38.0
76-77	27.658879254082315	33.0	26.0	35.0	2.0	37.0
78-79	27.085974933833583	33.0	25.5	35.0	2.0	36.5
80-81	26.49195229458895	32.5	24.5	35.0	2.0	36.0
82-83	25.850903618409184	32.0	22.5	34.5	2.0	35.5
84-85	25.22477206613034	31.5	20.0	34.0	2.0	35.0
86-87	24.592090694722756	31.0	18.0	34.0	2.0	35.0
88-89	23.925379206527765	31.0	10.0	34.0	2.0	35.0
90-91	23.167340356101576	30.0	3.5	34.0	2.0	35.0
92-93	22.33290454982241	29.5	2.0	34.0	2.0	35.0
94-95	21.40818577758246	29.0	2.0	34.0	2.0	35.0
96-97	20.49850703315372	29.0	2.0	33.5	2.0	34.5
98	19.862563747691965	27.0	2.0	33.0	2.0	34.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	162827.0
3	28796.0
4	22212.0
5	19251.0
6	18366.0
7	19035.0
8	21484.0
9	25146.0
10	28391.0
11	31239.0
12	35161.0
13	40797.0
14	34551.0
15	34314.0
16	37823.0
17	43546.0
18	48855.0
19	47986.0
20	50582.0
21	55772.0
22	61605.0
23	67984.0
24	76250.0
25	85675.0
26	97921.0
27	112205.0
28	130836.0
29	153979.0
30	183207.0
31	222665.0
32	278202.0
33	358810.0
34	486256.0
35	677248.0
36	914143.0
37	953137.0
38	413459.0
39	18544.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.56584304447085	17.335373031894616	13.189794434364199	24.90898948927033
2	16.136984377604442	18.916758211830917	38.358883949606195	26.587373460958446
3	20.21720764853655	22.219024603379957	27.690168672935496	29.873599075148
4	13.836991452475406	14.555566292987624	34.639500927074266	36.96794132746271
5	15.678059269187072	35.6802284865164	32.67965287271978	15.962059371576757
6	35.387067821167264	33.01683017716235	16.402293249059383	15.193808752610998
7	31.307126559876213	30.058077233763736	19.490619252073106	19.14417695428694
8	28.050141788486677	33.19479228683753	18.990390529283545	19.764675395392242
9	26.84236749048226	16.403178844265952	19.172580779006427	37.58187288624536
10-11	26.817298540365236	24.792298136503348	26.010640797201223	22.37976252593019
12-13	26.909274259180766	21.87574029853757	26.634659120121285	24.580326322160374
14-15	23.881783360517204	23.610354636798025	24.226219030679648	28.281642972005123
16-17	24.39126692642689	26.527966427756798	24.820022179880073	24.260744465936238
18-19	24.392081493213137	26.077020976590898	25.99150737735271	23.539390152843254
20-21	24.959892208901053	25.135692795368	25.911163427504206	23.993251568226736
22-23	24.690845331895005	24.79006962607296	25.84636927720395	24.672715764828084
24-25	24.5623432969899	24.981607305323163	25.968113750088918	24.48793564759802
26-27	24.495067091478294	25.058628780344943	26.111095292046777	24.335208836129986
28-29	24.415218726918095	25.47170686110044	25.854893677885265	24.2581807340962
30-31	24.623326647072485	25.21788730509834	25.706514030359962	24.452272017469216
32-33	24.45964759902384	25.295643398130597	25.70559546349459	24.539113539350975
34-35	24.75853429067416	25.160709626279846	25.81114550828756	24.269610574758435
36-37	24.68690181450768	25.10320014492567	25.864340398459802	24.345557642106847
38-39	24.62198582408191	25.335679358591857	25.74203423971096	24.300300577615275
40-41	24.74507761819496	25.304283474315163	25.680374270363576	24.270264637126303
42-43	24.61936345983179	25.212056830711354	25.600921445440882	24.56765826401598
44-45	24.678607901970054	25.159204383551533	25.560091537163732	24.602096177314685
46-47	24.840632484612062	25.260852314093206	25.594366524478353	24.304148676816382
48-49	24.779829585248077	25.305038319094752	25.509396936911344	24.405735158745827
50-51	24.441483257827194	25.510452398169726	25.63909920043841	24.40896514356467
52-53	24.671380442555876	25.496080911929326	25.377020008516364	24.45551863699843
54-55	24.59449933112229	25.26875279557902	25.570141464027984	24.566606409270708
56-57	24.619279048665327	25.330431220840488	25.547966035064352	24.50232369542983
58-59	24.866525537548025	25.252257587997235	25.453429203576285	24.42778767087846
60-61	24.686108268285764	25.32305955160337	25.69250827184576	24.298323908265104
62-63	24.44114646824744	25.471495257664255	25.624231097600525	24.463127176487777
64-65	24.587252690002416	25.412434063719243	25.511485834261393	24.48882741201695
66-67	24.644221234637286	25.34535651755761	25.425642456543596	24.584779791261504
68-69	24.520436423623394	25.358288089568642	25.50985978361453	24.611415703193433
70-71	24.66455339492805	25.171028550256157	25.584001233629657	24.58041682118614
72-73	24.692820379523358	25.172890972277816	25.57907604138973	24.5552126068091
74-75	24.47302760171431	25.615422487411006	25.410722886278375	24.500827024596315
76-77	24.665945722650022	25.225801494315835	25.379337698363578	24.728915084670568
78-79	24.596853522643638	25.202725121049557	25.57616970926942	24.624251647037383
80-81	24.67521922635313	25.30741757379813	25.464637498631987	24.552725701216758
82-83	24.784102832358997	25.142991797847326	25.524852358698535	24.548053011095142
84-85	24.64494868978932	25.31587516527179	25.41998260045618	24.61919354448271
86-87	24.61731883411188	25.37966753935582	25.464436078017027	24.53857754851527
88-89	24.542249864165495	25.206650879088304	25.40378284170316	24.847316415043043
90-91	24.656987365171513	25.0726886380091	25.470182753416292	24.800141243403097
92-93	24.8396968489757	25.264081274171048	25.41874797948449	24.477473897368764
94-95	24.818941999674866	25.107112909928308	25.459412388944425	24.6145327014524
96-97	24.61469035978593	25.053489036620906	25.639006852163398	24.692813751429764
98	24.760733440072936	25.229928765971827	25.207547704269594	24.801790089685642
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	646.0
1	493.0
2	560.0
3	778.0
4	632.5
5	589.5
6	977.5
7	2065.5
8	3047.0
9	2969.5
10	2388.0
11	2163.0
12	2455.0
13	2776.5
14	3174.0
15	3689.5
16	4081.5
17	4217.5
18	4298.0
19	4272.5
20	4038.5
21	3813.0
22	3698.5
23	3876.0
24	4353.5
25	5264.5
26	6731.5
27	9202.0
28	12964.5
29	18050.5
30	24754.5
31	33324.0
32	43454.5
33	54004.5
34	65893.0
35	78954.0
36	93038.5
37	107911.5
38	123268.0
39	138244.5
40	152967.0
41	169043.5
42	185544.0
43	201745.5
44	216696.5
45	233453.5
46	255802.0
47	276446.5
48	282527.5
49	277133.0
50	273625.0
51	272021.0
52	270711.0
53	271552.5
54	267441.5
55	257336.5
56	242275.0
57	224909.5
58	207135.0
59	185047.0
60	161705.0
61	137340.5
62	111901.0
63	86527.0
64	64386.5
65	48956.0
66	37649.5
67	28899.0
68	22224.5
69	17119.0
70	12997.0
71	9861.5
72	7753.0
73	5944.0
74	4552.0
75	3511.5
76	2440.0
77	1542.5
78	930.0
79	540.5
80	299.5
81	176.5
82	114.0
83	68.5
84	48.5
85	39.0
86	29.5
87	20.5
88	17.0
89	20.5
90	15.5
91	11.5
92	11.0
93	6.0
94	4.0
95	4.0
96	6.5
97	6.5
98	2.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.4604920091960658
2	0.047685733307533625
3	0.05652432005194925
4	0.053769435871871656
5	0.06388707598560901
6	0.06313276246011157
7	0.04237274238881255
8	0.018857838137435926
9	0.011445887843417631
10-11	0.014405748524989095
12-13	0.010421005335948287
14-15	0.014676317506961002
16-17	0.013807217140627
18-19	0.05449095315713007
20-21	0.08186761469665117
22-23	0.09842151695729602
24-25	0.06769963891339496
26-27	0.08280230754346321
28-29	0.08442572143529466
30-31	0.05613896422914077
32-33	0.031771357731549654
34-35	0.3901932682437285
36-37	0.6103708270883826
38-39	0.5592168913755727
40-41	0.5537153220754772
42-43	0.5270109834608561
44-45	0.5118837176506085
46-47	0.5018234709572894
48-49	0.49204199230600204
50-51	0.506250963389557
52-53	0.587323269260412
54-55	0.5574950887630242
56-57	0.515146943554391
58-59	0.4928946945522165
60-61	0.5057426216658523
62-63	0.5177952399536917
64-65	0.5369564433133386
66-67	0.5626031031802514
68-69	0.5164423950438322
70-71	0.46516547343012593
72-73	0.44901332511240916
74-75	0.4294913631101331
76-77	0.44362654265315027
78-79	0.4374444513680952
80-81	0.43472236342825654
82-83	0.45454769065274353
84-85	0.4212185115098405
86-87	0.4237602201283645
88-89	0.450185790700954
90-91	0.43409103580365543
92-93	0.42915520164768306
94-95	0.44050270077038367
96-97	0.4338122677616238
98	0.42900761856660746
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	6098260.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.89176623289333
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.89221570710077	37.30614051437615
2	14.690879061977814	15.246713240797199
3	5.548303871557055	8.637338624756872
4	2.5488176597892207	5.290506006882099
5	1.4485741468842919	3.7584535500566276
6	0.8618643703159132	2.6834198657335904
7	0.6012644204503171	2.1840470925114763
8	0.41307257595734675	1.7148052439038168
9	0.30556744643111955	1.427079104872723
>10	1.5641677039123596	14.55452251041688
>50	0.09102535698684873	3.2453318416582326
>100	0.033043231229903144	2.809791979139149
>500	6.022298292113022E-4	0.22977415278029886
>1k	5.388262540031229E-4	0.6450521030454158
>5k	3.1695661958229266E-5	0.09477014241937347
>10k+	3.1695661958229266E-5	0.17225402665013856
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	10473	0.171737512011623	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.63981201195095E-5
2	0.0	0.0	0.0	0.0	1.63981201195095E-5
3	0.0	0.0	0.0	0.0	1.63981201195095E-5
4	0.0	0.0	0.0	0.0	3.2796240239019E-5
5	0.0	0.0	0.0	0.0	3.2796240239019E-5
6	1.63981201195095E-5	0.0	0.0	0.0	4.9194360358528496E-5
7	1.63981201195095E-5	0.0	0.0	0.0	4.9194360358528496E-5
8	1.63981201195095E-5	0.0	0.0	0.0	4.9194360358528496E-5
9	1.63981201195095E-5	0.0	0.0	0.0	4.9194360358528496E-5
10-11	1.63981201195095E-5	0.0	0.0	0.0	8.199060059754749E-5
12-13	1.63981201195095E-5	0.0	0.0	0.0	1.7218026125484975E-4
14-15	1.63981201195095E-5	1.63981201195095E-5	0.0	1.63981201195095E-5	2.5417086185239724E-4
16-17	1.63981201195095E-5	1.63981201195095E-5	0.0	4.0995300298773745E-5	3.3616146244994473E-4
18-19	1.63981201195095E-5	1.63981201195095E-5	0.0	5.7393420418283246E-5	4.181520630474922E-4
20-21	1.63981201195095E-5	3.2796240239019E-5	0.0	6.5592480478038E-5	4.5914736334626597E-4
22-23	1.63981201195095E-5	3.2796240239019E-5	0.0	9.838872071705699E-5	4.919436035852849E-4
24-25	1.63981201195095E-5	4.9194360358528496E-5	0.0	1.6398120119509498E-4	5.24739843824304E-4
26-27	1.63981201195095E-5	4.9194360358528496E-5	8.19906005975475E-6	2.62369921912152E-4	5.739342041828324E-4
28-29	1.63981201195095E-5	4.9194360358528496E-5	1.63981201195095E-5	6.723229248998895E-4	5.985313843620967E-4
30-31	1.63981201195095E-5	4.9194360358528496E-5	1.63981201195095E-5	0.0022711396365520655	6.067304444218515E-4
32-33	1.63981201195095E-5	4.9194360358528496E-5	1.63981201195095E-5	0.006026309143919741	7.051191651389084E-4
34-35	1.63981201195095E-5	8.199060059754749E-5	1.63981201195095E-5	0.011216314161744498	7.21517285258418E-4
36-37	1.63981201195095E-5	9.838872071705699E-5	1.63981201195095E-5	0.01880044471701764	7.21517285258418E-4
38-39	1.63981201195095E-5	1.2298590089632124E-4	1.63981201195095E-5	0.030992447025872956	7.789107056767012E-4
40-41	1.63981201195095E-5	1.475830810755855E-4	1.63981201195095E-5	0.044340516803153686	8.035078858559655E-4
42-43	1.63981201195095E-5	1.803793213146045E-4	1.63981201195095E-5	0.05797555368252584	8.199060059754749E-4
44-45	3.2796240239019E-5	1.803793213146045E-4	1.63981201195095E-5	0.07261907494924782	8.936975465132677E-4
46-47	4.9194360358528496E-5	1.803793213146045E-4	1.63981201195095E-5	0.08797591444116847	9.838872071705699E-4
48-49	4.9194360358528496E-5	1.803793213146045E-4	1.63981201195095E-5	0.10266863006824897	0.001049479687648608
50-51	4.9194360358528496E-5	1.803793213146045E-4	1.63981201195095E-5	0.11802546956016963	0.0010658778077681174
52-53	4.9194360358528496E-5	1.9677744143411398E-4	1.63981201195095E-5	0.13364467897400242	0.0011314702882461555
54-55	4.9194360358528496E-5	1.9677744143411398E-4	1.63981201195095E-5	0.1497886282316595	0.0012626552492022314
56-57	4.9194360358528496E-5	1.9677744143411398E-4	1.63981201195095E-5	0.1679823425042553	0.0013036505495010052
58-59	4.9194360358528496E-5	1.9677744143411398E-4	1.63981201195095E-5	0.1861432605366121	0.001336446789740024
60-61	9.838872071705699E-5	2.131755615536235E-4	1.63981201195095E-5	0.2036810500044275	0.0013856411500985527
62-63	9.838872071705699E-5	2.2957368167313299E-4	1.63981201195095E-5	0.22115324699176486	0.001443034570516836
64-65	9.838872071705699E-5	2.2957368167313299E-4	1.63981201195095E-5	0.2394289518649582	0.0014594326906363455
66-67	9.838872071705699E-5	2.4597180179264247E-4	3.2796240239019E-5	0.25872134018556114	0.0015168261110546288
68-69	1.0658778077681175E-4	2.4597180179264247E-4	3.2796240239019E-5	0.2797519292388321	0.0015742195314729119
70-71	1.1478684083656649E-4	2.5417086185239724E-4	3.2796240239019E-5	0.3003643662290555	0.0016152148317716856
72-73	1.1478684083656649E-4	2.62369921912152E-4	3.2796240239019E-5	0.3201896934535425	0.0018775847536838376
74-75	1.1478684083656649E-4	2.62369921912152E-4	3.2796240239019E-5	0.3412612778071122	0.002000570654580159
76-77	1.1478684083656649E-4	2.62369921912152E-4	3.2796240239019E-5	0.36266902362313186	0.0020415659548789324
78-79	1.1478684083656649E-4	2.62369921912152E-4	3.2796240239019E-5	0.38676606113875106	0.002074362195117952
80-81	1.1478684083656649E-4	2.787680420316615E-4	3.2796240239019E-5	0.41251110972638094	0.0020907603152374612
82-83	1.31184960956076E-4	2.95166162151171E-4	3.2796240239019E-5	0.43699350306480866	0.0021399546755959896
84-85	1.475830810755855E-4	2.95166162151171E-4	3.2796240239019E-5	0.4610167490398901	0.0021481537356557445
86	1.475830810755855E-4	2.95166162151171E-4	3.2796240239019E-5	0.47966141161577236	0.0021481537356557445
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCTG	6550	0.0	25.791235	1
GTACATG	18625	0.0	25.678322	1
TACATGG	19030	0.0	24.213453	2
TACCTGG	7015	0.0	23.594404	2
ACATGGG	19705	0.0	22.895393	3
GAGTACA	14455	0.0	20.63499	1
CATGGGG	15180	0.0	20.447788	4
AGTACAT	13585	0.0	19.878569	2
GTATCAA	22760	0.0	18.86934	1
ACCTGGG	8915	0.0	17.331573	3
AGAGTAC	16760	0.0	17.23435	10-11
TCAACGC	25515	0.0	16.603739	4
TATCAAC	25770	0.0	16.43944	2
CAACGCA	25930	0.0	16.302637	5
ATCAACG	26195	0.0	16.190222	3
GTATAGG	1345	0.0	16.08507	1
AACGCAG	26585	0.0	16.000116	6
GTGTAGC	3305	0.0	15.87745	1
GTATATA	1445	0.0	15.609019	1
TATACCG	480	3.8198777E-11	15.282987	5
>>END_MODULE