FastQCFastQC Report
Mon 15 Aug 2016
SRR2921460_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921460_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6098260
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT109800.1800513589122143No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66810.10955584051844296No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG67150.026.5260331
GTACATG188000.024.9278011
TACCTGG70500.024.792462
TACATGG193100.023.5883332
ACATGGG199250.022.2890743
GAGTACA146350.020.8273491
AGTACAT140050.019.7378922
CATGGGG148250.019.7364164
GTATCAA239900.019.1378671
GTATAGG15950.018.8116381
ACCTGGG92400.018.4030513
AGAGTAC177450.017.91290710-11
GTGTAGC33500.016.9179941
TCAACGC270250.016.644344
ATGGGGG72050.016.605125
CAACGCA272800.016.4635035
TATCAAC279100.016.3372
ATCAACG280150.016.0899353
AACGCAG282550.016.0306056
CGTATAC4101.0713848E-815.0604593
Produced by FastQC (version 0.11.5)